EC: 2.6.1.42

branched-chain-amino-acid transaminase (branched-chain-amino-acid:2-oxoglutarate aminotransferase)

enzyme lambda metabolic reaction experiment
uniprot:O35854 [branched-chain-amino-acid transaminase(Enzyme) wildtype]

2_Oxoglutarate + L_Leucine -> 4_Methyl_2_oxopentanoate + L_Glutamate		 (( (V * A) ) / (Km + A)) buffer: -
PH: 8.2
Temperature: 37
uniprot:O35854 [branched-chain-amino-acid transaminase(Enzyme) wildtype]

L_Leucine + 2_Oxoglutarate -> 4_Methyl_2_oxopentanoate + L_Glutamate		 (( (V * A) ) / (Km + A)) buffer: -
PH: 8.2
Temperature: 37
uniprot:Q9LE06 [Pyridoxal phosphate]

2_Oxoglutarate + L_Leucine -> 4_Methyl_2_oxopentanoate + L_Glutamate		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM Tris
PH: 8
Temperature: 37
uniprot:Q9M439 [Pyridoxal phosphate]

L_Leucine + 2_Oxoglutarate -> 4_Methyl_2_oxopentanoate + L_Glutamate		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM Tris
PH: 8
Temperature: 37
uniprot:Q9M401 [Pyridoxal phosphate]

alpha_Ketovaline + L_Glutamate -> L_Valine + 2_Oxoglutarate		 (( (Vmax * A) ) / (Km + A)) buffer: 0.1 M Tris-HCl, 0.05 M L-aspartate, 5 mM DTT, 0.5 U/ml aspartate aminotransferase, 1 U/ml malate dehydrogenase, 50 µM NADH
PH: 8.3
Temperature: 33
uniprot:Q9M401 [Pyridoxal phosphate]

3_Methyl_2_oxopentanoate + L_Glutamate -> 2_Oxoglutarate + L_Isoleucine		 (( (Vmax * A) ) / (Km + A)) buffer: 0.1 M Tris-HCl, 0.05 M L-aspartate, 5 mM DTT, 0.5 U/ml aspartate aminotransferase, 1 U/ml malate dehydrogenase, 50 µM NADH
PH: 8.3
Temperature: 33
uniprot:Q9M401 [Pyridoxal phosphate]

L_Glutamate + 4_Methyl_2_oxopentanoate -> L_Leucine + 2_Oxoglutarate		 (( (Vmax * A) ) / (Km + A)) buffer: 0.1 M Tris-HCl, 0.05 M L-aspartate, 5 mM DTT, 0.5 U/ml aspartate aminotransferase, 1 U/ml malate dehydrogenase, 50 µM NADH
PH: 8.3
Temperature: 33
uniprot:P54690 [1,4-Dithiothreitol]

2_Oxoglutarate + L_Leucine -> L_Glutamate + 4_Methyl_2_oxopentanoate		 (( (( (Vmax * A) ) * B) ) / (( (( (KmB * A) ) + (KmA * B)) ) + (A * B))) buffer: 50 mM Potassium phosphate, 0.5 mg/ml Bovine serum albumin
PH: 7.8
Temperature: 37
uniprot:P54690 [1,4-Dithiothreitol]

2_Oxoglutarate + L_Isoleucine -> (S)_3_Methyl_2_oxopentanoate + L_Glutamate		 (( (( (Vmax * A) ) * B) ) / (( (( (KmB * A) ) + (KmA * B)) ) + (A * B))) buffer: 50 mM Potassium phosphate, 0.5 mg/ml Bovine serum albumin
PH: 7.8
Temperature: 37
uniprot:O35854 [1,4-Dithiothreitol]

2_Oxoglutarate + L_Valine -> alpha_Ketovaline + L_Glutamate		 (( (( (Vmax * A) ) * B) ) / (( (( (KmB * A) ) + (KmA * B)) ) + (A * B))) buffer: 50 mM Potassium phosphate, 0.5 mg/ml Bovine serum albumin
PH: 7.8
Temperature: 37
uniprot:P54690 [1,4-Dithiothreitol]

L_Valine + 2_Oxoglutarate -> alpha_Ketovaline + L_Glutamate		 (( (( (Vmax * A) ) * B) ) / (( (( (KmB * A) ) + (KmA * B)) ) + (A * B))) buffer: 50 mM Potassium phosphate, 0.5 mg/ml Bovine serum albumin
PH: 7.8
Temperature: 37
uniprot:O35854 [1,4-Dithiothreitol]

2_Oxoglutarate + L_Leucine -> 4_Methyl_2_oxopentanoate + L_Glutamate		 (( (( (Vmax * A) ) * B) ) / (( (( (KmB * A) ) + (KmA * B)) ) + (A * B))) buffer: 50 mM Potassium phosphate, 0.5 mg/ml Bovine serum albumin
PH: 7.8
Temperature: 37
uniprot:O35854 [1,4-Dithiothreitol]

2_Oxoglutarate + L_Isoleucine -> L_Glutamate + (S)_3_Methyl_2_oxopentanoate		 (( (( (Vmax * A) ) * B) ) / (( (( (KmB * A) ) + (KmA * B)) ) + (A * B))) buffer: 50 mM Potassium phosphate, 0.5 mg/ml Bovine serum albumin
PH: 7.8
Temperature: 37

Pathways

pathway id name
BioCyc:HUMAN_ILEUDEG-PWY isoleucine degradation
BioCyc:META_PWY-6628 superpathway of L-phenylalanine biosynthesis
BioCyc:META_PHESYN L-phenylalanine biosynthesis I
BioCyc:META_PWYQT-4450 aliphatic glucosinolate biosynthesis, side chain elongation cycle
BioCyc:META_ILEUDEG-PWY L-isoleucine degradation I
BioCyc:META_ALL-CHORISMATE-PWY superpathway of chorismate metabolism
BioCyc:META_PWY-5104 L-isoleucine biosynthesis IV
BioCyc:ECO_LEUSYN-PWY L-leucine biosynthesis
BioCyc:ECO_COMPLETE-ARO-PWY superpathway of aromatic amino acid biosynthesis
BioCyc:ECO_ILEUSYN-PWY L-isoleucine biosynthesis I (from threonine)
BioCyc:YEAST_PWY3O-4109 isoleucine degradation
BioCyc:ARA_VALDEG-PWY L-valine degradation I
BioCyc:MTBH37RV_LEUSYN-PWY L-leucine biosynthesis
BioCyc:MTBH37RV_ILEUSYN-PWY L-isoleucine biosynthesis I (from threonine)
BioCyc:MTBH37RV_PWY-3001 superpathway of L-isoleucine biosynthesis I
BioCyc:MTBH37RV_VALSYN-PWY L-valine biosynthesis
BioCyc:MTBCDC1551_ILEUDEG-PWY L-isoleucine degradation I
BioCyc:ECOO157_PWY-3001 isoleucine biosynthesis I
BioCyc:ECOO157_VALDEG-PWY valine degradation I
BioCyc:ECOL199310_ALANINE-VALINESYN-PWY alanine biosynthesis I
BioCyc:BSUB_LEUSYN-PWY L-leucine biosynthesis
BioCyc:ECOL316407_VALSYN-PWY valine biosynthesis
BioCyc:ECOL316407_ALL-CHORISMATE-PWY superpathway of chorismate metabolism
BioCyc:HUMAN_LEU-DEG2-PWY leucine degradation
BioCyc:META_PWY-1186 L-homomethionine biosynthesis
BioCyc:META_LEUSYN-PWY L-leucine biosynthesis
BioCyc:META_PWY-5078 L-isoleucine degradation II
BioCyc:META_VALDEG-PWY L-valine degradation I
BioCyc:META_THREOCAT-PWY superpathway of L-threonine metabolism
BioCyc:META_ILEUSYN-PWY L-isoleucine biosynthesis I (from threonine)
BioCyc:ECO_BRANCHED-CHAIN-AA-SYN-PWY superpathway of branched chain amino acid biosynthesis
BioCyc:ECO_THREOCAT-PWY superpathway of L-threonine metabolism
BioCyc:ECO_ALANINE-VALINESYN-PWY L-alanine biosynthesis I
BioCyc:YEAST_LEUSYN-PWY leucine biosynthesis
BioCyc:YEAST_PWY-5078 isoleucine degradation II
BioCyc:YEAST_PWY3O-4112 leucine degradation
BioCyc:ARA_ILEUSYN-PWY L-isoleucine biosynthesis I (from threonine)
BioCyc:ARA_LEU-DEG2-PWY L-leucine degradation I
BioCyc:ARA_BRANCHED-CHAIN-AA-SYN-PWY superpathway of branched chain amino acid biosynthesis
BioCyc:ARA_ILE-VAL-PWY superpathway of isoleucine and valine biosynthesis
BioCyc:ARA_ILEUDEG-PWY L-isoleucine degradation I
BioCyc:MTBH37RV_ALANINE-VALINESYN-PWY L-alanine biosynthesis I
BioCyc:MTBCDC1551_VALSYN-PWY L-valine biosynthesis
BioCyc:MTBCDC1551_BRANCHED-CHAIN-AA-SYN-PWY superpathway of branched amino acid biosynthesis
BioCyc:MTBCDC1551_PWY-5057 L-valine degradation II
BioCyc:ECOO157_PWY-5103 isoleucine biosynthesis III
BioCyc:ECOO157_VALSYN-PWY valine biosynthesis
BioCyc:ECOL199310_PWY-3001 isoleucine biosynthesis I
BioCyc:ECOL199310_ILEUSYN-PWY isoleucine biosynthesis I (from threonine)
BioCyc:ECOL199310_VALSYN-PWY valine biosynthesis
BioCyc:ECOL199310_LEUSYN-PWY leucine biosynthesis
BioCyc:MOUSE_PWY3DJ-0 isoleucine degradation
BioCyc:MOUSE_PWY3DJ-6 Leucine Catabolism
BioCyc:MOUSE_LEU-DEG2-PWY leucine degradation I
BioCyc:BSUB_VALDEG-PWY L-valine degradation I
BioCyc:BSUB_ALANINE-VALINESYN-PWY L-alanine biosynthesis I
BioCyc:ECOL316407_COMPLETE-ARO-PWY superpathway of phenylalanine, tyrosine, and tryptophan biosynthesis
BioCyc:ECOL316407_ALANINE-VALINESYN-PWY alanine biosynthesis I
BioCyc:ECOL316407_PWY0-1061 superpathway of alanine biosynthesis
BioCyc:HUMAN_VALDEG-PWY valine degradation
BioCyc:META_PWY0-1061 superpathway of L-alanine biosynthesis
BioCyc:META_ALANINE-VALINESYN-PWY L-alanine biosynthesis I
BioCyc:META_PWY-5076 L-leucine degradation III
BioCyc:META_VALSYN-PWY L-valine biosynthesis
BioCyc:META_BRANCHED-CHAIN-AA-SYN-PWY superpathway of branched chain amino acid biosynthesis
BioCyc:META_COMPLETE-ARO-PWY superpathway of aromatic amino acid biosynthesis
BioCyc:META_PWY-5057 L-valine degradation II
BioCyc:META_PWY-5101 L-isoleucine biosynthesis II
BioCyc:ECO_PWY0-1061 superpathway of L-alanine biosynthesis
BioCyc:ECO_VALSYN-PWY L-valine biosynthesis
BioCyc:YEAST_PWY-5076 leucine degradation III
BioCyc:YEAST_ILEUSYN-PWY isoleucine biosynthesis
BioCyc:ARA_LEUSYN-PWY L-leucine biosynthesis
BioCyc:MTBH37RV_BRANCHED-CHAIN-AA-SYN-PWY superpathway of branched chain amino acid biosynthesis
BioCyc:MTBH37RV_ILEUDEG-PWY L-isoleucine degradation I
BioCyc:MTBCDC1551_ILEUSYN-PWY L-isoleucine biosynthesis I (from threonine)
BioCyc:MTBCDC1551_ALANINE-VALINESYN-PWY L-alanine biosynthesis I
BioCyc:MTBCDC1551_LEUSYN-PWY L-leucine biosynthesis
BioCyc:MTBCDC1551_VALDEG-PWY L-valine degradation I
BioCyc:HPY_VALSYN-PWY L-valine biosynthesis
BioCyc:ECOO157_ILEUSYN-PWY isoleucine biosynthesis I (from threonine)
BioCyc:ECOL199310_BRANCHED-CHAIN-AA-SYN-PWY superpathway of leucine, valine, and isoleucine biosynthesis
BioCyc:ECOL199310_ILEUDEG-PWY isoleucine degradation I
BioCyc:MOUSE_VALDEG-PWY valine degradation I
BioCyc:BSUB_BRANCHED-CHAIN-AA-SYN-PWY superpathway of branched chain amino acid biosynthesis
BioCyc:BSUB_ILEUDEG-PWY L-isoleucine degradation I
BioCyc:BSUB_ILEUSYN-PWY L-isoleucine biosynthesis I (from threonine)
BioCyc:ECOL316407_ILEUSYN-PWY isoleucine biosynthesis I (from threonine)
BioCyc:ECOL316407_BRANCHED-CHAIN-AA-SYN-PWY superpathway of leucine, valine, and isoleucine biosynthesis
BioCyc:META_LEU-DEG2-PWY L-leucine degradation I
BioCyc:META_PWY-3001 superpathway of L-isoleucine biosynthesis I
BioCyc:ECO_ALL-CHORISMATE-PWY superpathway of chorismate metabolism
BioCyc:ECO_PHESYN L-phenylalanine biosynthesis I
BioCyc:YEAST_BRANCHED-CHAIN-AA-SYN-PWY superpathway of leucine, valine, and isoleucine biosynthesis
BioCyc:YEAST_PWY-5057 valine degradation
BioCyc:YEAST_VALSYN-PWY valine biosynthesis
BioCyc:ARA_PWY-3001 superpathway of L-isoleucine biosynthesis I
BioCyc:ARA_PWY-1186 L-homomethionine biosynthesis
BioCyc:ARA_PWYQT-4450 aliphatic glucosinolate biosynthesis, side chain elongation cycle
BioCyc:ARA_VALSYN-PWY L-valine biosynthesis
BioCyc:MTBH37RV_VALDEG-PWY L-valine degradation I
BioCyc:ECOO157_ALANINE-VALINESYN-PWY alanine biosynthesis I
BioCyc:ECOO157_BRANCHED-CHAIN-AA-SYN-PWY superpathway of leucine, valine, and isoleucine biosynthesis
BioCyc:MOUSE_PWY3DJ-86 valine degradation
BioCyc:BSUB_VALSYN-PWY L-valine biosynthesis
BioCyc:ECOL316407_LEU-DEG2-PWY leucine degradation I
BioCyc:ECOL316407_PHESYN phenylalanine biosynthesis I
BioCyc:ECOL316407_LEUSYN-PWY leucine biosynthesis
BioCyc:ECOL316407_THREOCAT-PWY superpathway of threonine metabolism
PathBank:SMP0000810 L-Alanine Metabolism
PathBank:SMP0000827 Phenylalanine Biosynthesis
PathBank:SMP0000831 Leucine Biosynthesis
PathBank:SMP0000832 Valine Biosynthesis
PathBank:SMP0000838 Isoleucine Biosynthesis
PathBank:SMP0000938 Phenylalanine Metabolism
PathBank:SMP0000994 Secondary Metabolites: Valine and L-Leucine Biosynthesis from Pyruvate
PathBank:SMP0000996 Secondary Metabolites: Leucine Biosynthesis
PlantCyc:ARA_VALSYN-PWY L-valine biosynthesis
PlantCyc:ARA_VALDEG-PWY L-valine degradation I
PlantCyc:PLANT_PWY-1186 L-homomethionine biosynthesis
PlantCyc:PLANT_ILE-VAL-PWY superpathway of isoleucine and valine biosynthesis
PlantCyc:PLANT_ILEUDEG-PWY L-isoleucine degradation I
PlantCyc:PLANT_VALDEG-PWY L-valine degradation I
PlantCyc:ARA_PWY-3001 superpathway of L-isoleucine biosynthesis I
PlantCyc:ARA_ILEUDEG-PWY L-isoleucine degradation I
PlantCyc:ARA_PWY-1186 L-homomethionine biosynthesis
PlantCyc:PLANT_PWYQT-4450 aliphatic glucosinolate biosynthesis, side chain elongation cycle
PlantCyc:ARA_BRANCHED-CHAIN-AA-SYN-PWY superpathway of branched chain amino acid biosynthesis
PlantCyc:ARA_LEUSYN-PWY L-leucine biosynthesis
PlantCyc:ARA_ILEUSYN-PWY L-isoleucine biosynthesis I (from threonine)
PlantCyc:ARA_LEU-DEG2-PWY L-leucine degradation I
PlantCyc:PLANT_LEUSYN-PWY L-leucine biosynthesis
PlantCyc:PLANT_VALSYN-PWY L-valine biosynthesis
PlantCyc:PLANT_LEU-DEG2-PWY L-leucine degradation I
PlantCyc:PLANT_PWY-3001 superpathway of L-isoleucine biosynthesis I
PlantCyc:ARA_PWYQT-4450 aliphatic glucosinolate biosynthesis, side chain elongation cycle
PlantCyc:PLANT_ILEUSYN-PWY L-isoleucine biosynthesis I (from threonine)
Reactome:R-BTA-1430728 Metabolism
Reactome:R-BTA-71291 Amino acid and derivative metabolism
Reactome:R-CEL-70895 Branched-chain amino acid catabolism
Reactome:R-HSA-70895 Branched-chain amino acid catabolism
Reactome:R-MMU-1430728 Metabolism
Reactome:R-MMU-70895 Branched-chain amino acid catabolism
Reactome:R-RNO-70895 Branched-chain amino acid catabolism
Reactome:R-SCE-71291 Amino acid and derivative metabolism
Reactome:R-SPO-1430728 Metabolism
Reactome:R-BTA-70895 Branched-chain amino acid catabolism
Reactome:R-DDI-71291 Amino acid and derivative metabolism
Reactome:R-SCE-1430728 Metabolism
Reactome:R-SCE-70895 Branched-chain amino acid catabolism
Reactome:R-SPO-71291 Amino acid and derivative metabolism
Reactome:R-CEL-71291 Amino acid and derivative metabolism
Reactome:R-HSA-71291 Amino acid and derivative metabolism
Reactome:R-MMU-71291 Amino acid and derivative metabolism
Reactome:R-RNO-1430728 Metabolism
Reactome:R-RNO-71291 Amino acid and derivative metabolism
Reactome:R-CEL-1430728 Metabolism
Reactome:R-DDI-1430728 Metabolism
Reactome:R-DDI-70895 Branched-chain amino acid catabolism
Reactome:R-HSA-1430728 Metabolism
Reactome:R-SPO-70895 Branched-chain amino acid catabolism
WikiPathways:WP4686 Leucine, isoleucine and valine metabolism
WikiPathways:WP5220 Metabolic reprogramming in pancreatic cancer
PathBank:SMP0120662 3-Methylcrotonyl-CoA Carboxylase Deficiency Type I
PathBank:SMP0120806 3-Hydroxyisobutyric Aciduria
PathBank:SMP0000032 Valine, Leucine, and Isoleucine Degradation
PathBank:SMP0000173 beta-Ketothiolase Deficiency
PathBank:SMP0000199 Maple Syrup Urine Disease
PathBank:SMP0000384 Methylmalonate Semialdehyde Dehydrogenase Deficiency
PathBank:SMP0000141 3-Methylglutaconic Aciduria Type IV
PathBank:SMP0000524 Isovaleric Acidemia
PathBank:SMP0002433 Leucine Degradation
PathBank:SMP0120661 3-Hydroxy-3-methylglutaryl-CoA Lyase Deficiency
PathBank:SMP0120665 3-Methylglutaconic Aciduria Type IV
PathBank:SMP0120745 Methylmalonic Aciduria
PathBank:SMP0120757 Propionic Acidemia
PathBank:SMP0120805 3-Hydroxyisobutyric Acid Dehydrogenase Deficiency
PathBank:SMP0000138 3-Hydroxy-3-methylglutaryl-CoA Lyase Deficiency
PathBank:SMP0000140 3-Methylglutaconic Aciduria Type III
PathBank:SMP0000238 Isovaleric Aciduria
PathBank:SMP0000523 Isobutyryl-CoA Dehydrogenase Deficiency
PathBank:SMP0002390 Isoleucine Degradation
PathBank:SMP0120660 2-Methyl-3-hydroxybutryl-CoA Dehydrogenase Deficiency
PathBank:SMP0120664 3-Methylglutaconic Aciduria Type III
PathBank:SMP0120736 Maple Syrup Urine Disease
PathBank:SMP0120808 Isovaleric Acidemia
PathBank:SMP0121204 L-Homomethionine Biosynthesis
PathBank:SMP0000137 2-Methyl-3-hydroxybutyryl-CoA Dehydrogenase Deficiency
PathBank:SMP0000237 3-Methylcrotonyl-CoA Carboxylase Deficiency Type I
PathBank:SMP0000200 Methylmalonic Aciduria
PathBank:SMP0000521 3-Hydroxyisobutyric Acid Dehydrogenase Deficiency
PathBank:SMP0002388 Valine Degradation
PathBank:SMP0002460 Valine Degradation
PathBank:SMP0120663 3-Methylglutaconic Aciduria Type I
PathBank:SMP0120679 beta-Ketothiolase Deficiency
PathBank:SMP0120723 Isovaleric Aciduria
PathBank:SMP0120743 Methylmalonate Semialdehyde Dehydrogenase Deficiency
PathBank:SMP0120807 Isobutyryl-CoA Dehydrogenase Deficiency
PathBank:SMP0000236 Propionic Acidemia
PathBank:SMP0000139 3-Methylglutaconic Aciduria Type I
PathBank:SMP0000522 3-Hydroxyisobutyric Aciduria
PathBank:SMP0002389 Leucine Degradation
PathBank:SMP0002431 Isoleucine Degradation
PathBank:SMP0087327 Valine, Leucine, and Isoleucine Degradation