uniprot:Q57843 [fructose-bisphosphate aldolase(Enzyme) wildtype MJ0400 His-tagged]
D_Fructose_1,6_bisphosphate -> D_Glyceraldehyde_3_phosphate + Glycerone_phosphate |
(( (Vmax * A) ) / (Km + A)) |
|
buffer: 50 mM Tris/HCl, 1 mM Dithiothreitol, 0.4 mM NADH, 7.75 U/ml Glycerol 3-phosphate dehydrogenase, 22.5 U/ml Triose phosphate isomerase
PH: 7.5
Temperature: 50 |
uniprot:P14540 [fructose-bisphosphate aldolase(Enzyme) wildtype]
D_Fructose_1,6_bisphosphate -> Glycerone_phosphate + D_Glyceraldehyde_3_phosphate |
(( (( (FBA1 * FBAkcat) ) * (( (F16bP / FBAKf16bp) ) - (( (DHAP * GAP) ) / (FBAKeq * FBAKf16bp)))) ) / (( (( (( (( (1 + (DHAP / FBAKdhap)) ) + (F16bP / FBAKf16bp)) ) + (GAP / FBAKgap)) ) + (( (DHAP * GAP) ) / (FBAKdhap * FBAKgap))) ) + (( (F16bP * GAP) ) / (FBAKf16bp * FBAKigap)))) |
|
buffer: *
PH: 7
Temperature: 36 |
uniprot:P00883 [fructose-bisphosphate aldolase(Enzyme) wildtype aldolase A]
D_Fructose_1,6_bisphosphate -> Glycerone_phosphate + D_Glyceraldehyde_3_phosphate |
(( (( (E * kcat) ) * S) ) / (Km + S)) |
|
buffer: 50 mM Triethanolamine-HCl, 1 mM EDTA, 1 mM beta-Mercaptoethanol, 12 % PEG
PH: 8.5
Temperature: 20 |
uniprot:P00883 [fructose-bisphosphate aldolase(Enzyme) wildtype aldolase A]
D_Fructose_1,6_bisphosphate -> Glycerone_phosphate + D_Glyceraldehyde_3_phosphate |
(( (( (E * kcat) ) * S) ) / (Km + S)) |
|
buffer: 50 mM Triethanolamine-HCl, 1 mM EDTA, 1 mM beta-Mercaptoethanol
PH: 8.5
Temperature: 20 |
uniprot:P00883 [fructose-bisphosphate aldolase(Enzyme) wildtype aldolase A]
D_Fructose_1_phosphate -> D_Glyceraldehyde + Glycerone_phosphate |
(( (( (E * kcat) ) * S) ) / (Km + S)) |
|
buffer: 50 mM Triethanolamine-HCl, 1 mM EDTA, 1 mM beta-Mercaptoethanol
PH: 8.5
Temperature: 20 |
uniprot:P00883 [fructose-bisphosphate aldolase(Enzyme) wildtype aldolase A]
D_Fructose_1_phosphate -> Glycerone_phosphate + D_Glyceraldehyde |
(( (( (E * kcat) ) * S) ) / (Km + S)) |
|
buffer: 50 mM Triethanolamine-HCl, 1 mM EDTA, 1 mM beta-Mercaptoethanol, 12 % PEG
PH: 8.5
Temperature: 20 |
uniprot:P00883 [fructose-bisphosphate aldolase(Enzyme) wildtype aldolase A]
D_Fructose_1_phosphate -> D_Glyceraldehyde + Glycerone_phosphate |
(( (( (E * kcat) ) * S) ) / (Km + S)) |
|
buffer: 50 mM Triethanolamine-HCl, 1 mM EDTA, 1 mM beta-Mercaptoethanol
PH: 8.5
Temperature: 20 |
uniprot:P29356 [fructose-bisphosphate aldolase(Enzyme) wildtype class I]
D_Fructose_1,6_bisphosphate -> Glycerone_phosphate + D_Glyceraldehyde_3_phosphate |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 100 mM Tris-HCl, 1 U/ml Triosephosphate isomerase, 1 U/ml Glycerol-3-phosphate dehydrogenase, 4.5 mM MgCl2
PH: 7.5
Temperature: 36 |
uniprot:P16096 [fructose-bisphosphate aldolase(Enzyme) wildtype class I]
D_Fructose_1,6_bisphosphate -> D_Glyceraldehyde_3_phosphate + Glycerone_phosphate |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 100 mM Tris-HCl, 1 U/ml Triosephosphate isomerase, 1 U/ml Glycerol-3-phosphate dehydrogenase, 4.5 mM MgCl2
PH: 7.5
Temperature: 36 |
uniprot:Q9XGL3 [fructose-bisphosphate aldolase(Enzyme) wildtype class II]
D_Fructose_1,6_bisphosphate -> D_Glyceraldehyde_3_phosphate + Glycerone_phosphate |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 100 mM Tris-HCl, 1 U/ml Triosephosphate isomerase, 1 U/ml Glycerol-3-phosphate dehydrogenase, 4.5 mM MgCl2, 1 mM EDTA
PH: 7.5
Temperature: 36 |
uniprot:Q9XGL3 [fructose-bisphosphate aldolase(Enzyme) wildtype class II]
D_Fructose_1,6_bisphosphate -> D_Glyceraldehyde_3_phosphate + Glycerone_phosphate |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 100 mM Tris-HCl, 1 U/ml Triosephosphate isomerase, 1 U/ml Glycerol-3-phosphate dehydrogenase, 4.5 mM MgCl2, 1 mM EDTA
PH: 7.5
Temperature: 36 |
uniprot:P16096 [fructose-bisphosphate aldolase(Enzyme) wildtype class I]
D_Fructose_1_phosphate -> Glycerone_phosphate + D_Glyceraldehyde |
(( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) |
|
buffer: 100 mM Tris-HCl, 1 U/ml Triosephosphate isomerase, 1 U/ml Glycerol-3-phosphate dehydrogenase, 4.5 mM MgCl2
PH: 7.5
Temperature: 36 |
uniprot:P29356 [fructose-bisphosphate aldolase(Enzyme) wildtype class I]
Sedoheptulose_1,7_bisphosphate -> Glycerone_phosphate + D_Erythrose_4_phosphate |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 100 mM Tris-HCl, 1 U/ml Triosephosphate isomerase, 1 U/ml Glycerol-3-phosphate dehydrogenase, 4.5 mM MgCl2
PH: 7.5
Temperature: 36 |
uniprot:P16096 [fructose-bisphosphate aldolase(Enzyme) wildtype class I]
Sedoheptulose_1,7_bisphosphate -> Glycerone_phosphate + D_Erythrose_4_phosphate |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 100 mM Tris-HCl, 1 U/ml Triosephosphate isomerase, 1 U/ml Glycerol-3-phosphate dehydrogenase, 4.5 mM MgCl2
PH: 7.5
Temperature: 36 |
uniprot:P29356 [fructose-bisphosphate aldolase(Enzyme) wildtype class I]
D_Fructose_1_phosphate -> Glycerone_phosphate + D_Glyceraldehyde |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 100 mM Tris-HCl, 1 U/ml Triosephosphate isomerase, 1 U/ml Glycerol-3-phosphate dehydrogenase, 4.5 mM MgCl2
PH: 7.5
Temperature: 36 |
uniprot:Q9XGL3 [fructose-bisphosphate aldolase(Enzyme) wildtype class II]
Sedoheptulose_1,7_bisphosphate -> Glycerone_phosphate + D_Erythrose_4_phosphate |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 100 mM Tris-HCl, 1 U/ml Triosephosphate isomerase, 1 U/ml Glycerol-3-phosphate dehydrogenase, 4.5 mM MgCl2, 1 mM EDTA
PH: 7.5
Temperature: 36 |
uniprot:Q9XGL3 [fructose-bisphosphate aldolase(Enzyme) wildtype class II]
Sedoheptulose_1,7_bisphosphate -> Glycerone_phosphate + D_Erythrose_4_phosphate |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 100 mM Tris-HCl, 1 U/ml Triosephosphate isomerase, 1 U/ml Glycerol-3-phosphate dehydrogenase, 4.5 mM MgCl2, 1 mM EDTA
PH: 7.5
Temperature: 36 |
uniprot:P14540 [fructose-bisphosphate aldolase(Enzyme) wildtype]
D_Fructose_1,6_bisphosphate -> Glycerone_phosphate + D_Glyceraldehyde_3_phosphate |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 300 mM potassium, 245 mM glutamate, 50 mM phosphate, 20 mM sodium, 2 mM magnesium, 2.5-10 mM sulphate, 0.5 mM calcium, 0.15 mM NADH, 1.8 U/ml Triosephosphate isomerase, 0.6 U/ml Glycerol 3-phosphate dehydrogenase
PH: 6.8
Temperature: 30 |
uniprot:P14223 [fructose-bisphosphate aldolase(Enzyme) wildtype]
D_Fructose_1,6_bisphosphate -> D_Glyceraldehyde_3_phosphate + Glycerone_phosphate |
(( (( (( (( (Vpf * VPFvALD) ) * f16bpPF) ) / (Kf16bpPFvALD * Vpf)) ) * (1 - (( (( (dhapPF * gapPF) ) / f16bpPF) ) / (Vpf * KeqPFvALD)))) ) / (( (( (( (1 + (dhapPF / (KdhapPFvALD * Vpf))) ) + (gapPF / (KgapPFvALD * Vpf))) ) + (f16bpPF / (Kf16bpPFvALD * Vpf))) ) + (( (dhapPF * gapPF) ) / (( (( (KgapPFvALD * KdhapPFvALD) ) * Vpf) ) * Vpf)))) |
|
buffer: *
PH: 7
Temperature: 36 |
uniprot:Q980K6 [fructose-bisphosphate aldolase(Enzyme) wildtype SSO0286]
D_Glyceraldehyde_3_phosphate + Glycerone_phosphate -> D_Fructose_1,6_bisphosphate |
(( (( (Vmf * (A / KmA)) ) * (B / KmB)) ) / (( (( (1 + (A / KmA)) ) + (B / KmB)) ) + (( (( (A * B) ) / KmA) ) * KmB))) |
|
buffer: 100 mM Tris/HCl, 20 mM MgCl2, 1 mM NADP+, 9.2 µg/ml Glucose 6-phosphate dehydrogenase from Thermotoga maritima, 15.2 µg/ml Glucose 6-phosphate isomerase from Thermoproteus tenax
PH: 6.5
Temperature: 70 |
uniprot:I3EBM6 [fructose-bisphosphate aldolase(Enzyme) wildtype isozyme FBAc]
D_Glyceraldehyde_3_phosphate + Glycerone_phosphate -> D_Fructose_1,6_bisphosphate |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 50 mM Tris/HCl, 0.25 mM NADP+, 200 mM KCl, 2 mM MnCl2, 5 U/ml phosphoglucoseisomerase, 5 U/ml glucose 6-phosphate dehydrogenase, 5 U/ml fructose-bisphosphatase
PH: 7.5
Temperature: 50 |
uniprot:I3EBM6 [fructose-bisphosphate aldolase(Enzyme) wildtype isozyme FBAc]
D_Fructose_1,6_bisphosphate -> Glycerone_phosphate + D_Glyceraldehyde_3_phosphate |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 50 mM Tris/HCl, 0.25 mM NADH, 5 U/ml alpha-glycerol 3-phosphate dehydrogenase
PH: 7.5
Temperature: 50 |
uniprot:I3DTM2 [fructose-bisphosphate aldolase(Enzyme) wildtype isozyme FBAp]
D_Fructose_1,6_bisphosphate -> D_Glyceraldehyde_3_phosphate + Glycerone_phosphate |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 50 mM Tris/HCl, 0.25 mM NADH, 5 U/ml alpha-glycerol 3-phosphate dehydrogenase
PH: 7.5
Temperature: 50 |
uniprot:I3DTM2 [fructose-bisphosphate aldolase(Enzyme) wildtype isozyme FBAp]
Glycerone_phosphate + D_Glyceraldehyde_3_phosphate -> D_Fructose_1,6_bisphosphate |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 50 mM Tris/HCl, 0.25 mM NADP+, 200 mM KCl, 2 mM MnCl2, 5 U/ml phosphoglucoseisomerase, 5 U/ml glucose 6-phosphate dehydrogenase, 5 U/ml fructose-bisphosphatase
PH: 7.5
Temperature: 50 |
uniprot:I3EBM6 [fructose-bisphosphate aldolase(Enzyme) wildtype isozyme FBAc]
D_Glyceraldehyde_3_phosphate + Glycerone_phosphate -> D_Fructose_1,6_bisphosphate |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 50 mM Tris/HCl, 0.25 mM NADP+, 200 mM KCl, 2 mM MnCl2, 5 U/ml phosphoglucoseisomerase, 5 U/ml glucose 6-phosphate dehydrogenase, 5 U/ml fructose-bisphosphatase
PH: 7.5
Temperature: 50 |
uniprot:I3DTM2 [fructose-bisphosphate aldolase(Enzyme) wildtype isozyme FBAp]
Glycerone_phosphate + D_Glyceraldehyde_3_phosphate -> D_Fructose_1,6_bisphosphate |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 50 mM Tris/HCl, 0.25 mM NADP+, 200 mM KCl, 2 mM MnCl2, 5 U/ml phosphoglucoseisomerase, 5 U/ml glucose 6-phosphate dehydrogenase, 5 U/ml fructose-bisphosphatase
PH: 7.5
Temperature: 50 |
uniprot:P14540 [fructose-bisphosphate aldolase(Enzyme) wildtype isozyme Fba1p, C-terminal GST-tag]
D_Fructose_1,6_bisphosphate -> D_Glyceraldehyde_3_phosphate + Glycerone_phosphate |
(( (( (kcat * E) ) * S) ) / (Km + S)) |
|
buffer: 100 mM MES, 100 mM KCl, 5 mM MgCl2, 0.15 mM NADH, 50 U/ml triose phosphate isomerase, 4.3 U/ml glycerol-3-phosphate dehydrogenase
PH: 6.5
Temperature: 30 |
- [fructose-bisphosphate aldolase(Enzyme) wildtype]
beta_D_Fructose_1,6_bisphosphate -> D_Glyceraldehyde_3_phosphate + Glycerone_phosphate |
(( (( (cytosol * Vmax_5) ) * (( (F16bP / Kf16bp_5) ) - (( (DHAP * GAP) ) / (Kf16bp_5 * Keq_5)))) ) / (( (( (( (( (1 + (F16bP / Kf16bp_5)) ) + (DHAP / Kdhap_5)) ) + (GAP / Kgap_5)) ) + (( (F16bP * GAP) ) / (Kf16bp_5 * Kigap_5))) ) + (( (DHAP * GAP) ) / (Kdhap_5 * Kgap_5)))) |
|
buffer: *
PH: 7
Temperature: 36 |
- [fructose-bisphosphate aldolase(Enzyme) wildtype]
beta_D_Fructose_1,6_bisphosphate -> Glycerone_phosphate + D_Glyceraldehyde_3_phosphate |
(( (( (( (cytosol * VmALD) ) / KmALDF16P) ) * (F16P - (( (( (( (( (( (KeqTPI / (1 + KeqTPI)) ) * TRIO) ) * 1) ) / (1 + KeqTPI)) ) * TRIO) ) / KeqALD))) ) / (( (( (( (( (1 + (F16P / KmALDF16P)) ) + (( (( (KeqTPI / (1 + KeqTPI)) ) * TRIO) ) / KmALDGAP)) ) + (( (( (1 / (1 + KeqTPI)) ) * TRIO) ) / KmALDDHAP)) ) + (( (( (( (( (( (KeqTPI / (1 + KeqTPI)) ) * TRIO) ) * 1) ) / (1 + KeqTPI)) ) * TRIO) ) / (KmALDGAP * KmALDDHAP))) ) + (( (( (( (F16P * KeqTPI) ) / (1 + KeqTPI)) ) * TRIO) ) / (KmALDGAPi * KmALDF16P)))) |
|
buffer: *
PH: 7
Temperature: 36 |
- [fructose-bisphosphate aldolase(Enzyme) wildtype]
D_Fructose_1,6_bisphosphate -> Glycerone_phosphate + D_Glyceraldehyde_3_phosphate |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 50 mM Tris-HC, 3 mM MgCl2, 0.15 mM NADH, 1 unit/ml triosephosphate isomerase, 0.1 unit/ ml glycerol-3-phosphate dehydrogenase
PH: 8
Temperature: 30 |
uniprot:P05062 [fructose-bisphosphate aldolase(Enzyme) wildtype aldolase B]
D_Fructose_1,6_bisphosphate -> Glycerone_phosphate + D_Glyceraldehyde_3_phosphate |
(( (( (E * kcat) ) * S) ) / (Km + S)) |
|
buffer: 100 mM Tris/HCl, 0.2 mM NADH, 0.5 mM EDTA, 10 µg/ml alpha-glycerol-phosphate dehydrogenase/triose phosphate isomerase
PH: 7.5
Temperature: 30 |
uniprot:P05062 [fructose-bisphosphate aldolase(Enzyme) wildtype aldolase B]
D_Fructose_1_phosphate -> D_Glyceraldehyde + Glycerone_phosphate |
(( (( (E * kcat) ) * S) ) / (Km + S)) |
|
buffer: 100 mM Tris/HCl, 0.2 mM NADH, 0.5 mM EDTA, 10 µg/ml alpha-glycerol-phosphate dehydrogenase/triose phosphate isomerase
PH: 7.5
Temperature: 30 |
uniprot:P05062 [fructose-bisphosphate aldolase(Enzyme) mutant aldolase B R303W]
D_Fructose_1,6_bisphosphate -> Glycerone_phosphate + D_Glyceraldehyde_3_phosphate |
(( (( (E * kcat) ) * S) ) / (Km + S)) |
|
buffer: 100 mM Tris/HCl, 0.2 mM NADH, 0.5 mM EDTA, 10 µg/ml alpha-glycerol-phosphate dehydrogenase/triose phosphate isomerase
PH: 7.5
Temperature: 30 |
uniprot:P05062 [fructose-bisphosphate aldolase(Enzyme) mutant aldolase B R303Q]
D_Fructose_1,6_bisphosphate -> D_Glyceraldehyde_3_phosphate + Glycerone_phosphate |
(( (( (E * kcat) ) * S) ) / (Km + S)) |
|
buffer: 100 mM Tris/HCl, 0.2 mM NADH, 0.5 mM EDTA, 10 µg/ml alpha-glycerol-phosphate dehydrogenase/triose phosphate isomerase
PH: 7.5
Temperature: 30 |
uniprot:P05062 [fructose-bisphosphate aldolase(Enzyme) mutant aldolase B R303Q]
D_Fructose_1_phosphate -> Glycerone_phosphate + D_Glyceraldehyde |
(( (( (E * kcat) ) * S) ) / (Km + S)) |
|
buffer: 100 mM Tris/HCl, 0.2 mM NADH, 0.5 mM EDTA, 10 µg/ml alpha-glycerol-phosphate dehydrogenase/triose phosphate isomerase
PH: 7.5
Temperature: 30 |
uniprot:P05064 [fructose-bisphosphate aldolase(Enzyme) wildtype]
D_Fructose_1,6_bisphosphate -> Glycerone_phosphate + D_Glyceraldehyde_3_phosphate |
(( (V * A) ) / (Km + A)) |
|
buffer: 50 mM HEPES, 100 mM KCl, 10 mM NH2PO4, 10 mM MgCl2
PH: 7.4
Temperature: 37 |
- [fructose-bisphosphate aldolase(Enzyme) wildtype]
D_Fructose_1_phosphate -> D_Glyceraldehyde + Glycerone_phosphate |
(( (V * A) ) / (Km + A)) |
|
buffer: 0.06 M Tris-HCl, 0.28 mM NADH, 20 µg glyceraldehyde-3-phosphate dehydrogenase/triosephosphate isomerase
PH: 7.5
Temperature: 36 |
- [fructose-bisphosphate aldolase(Enzyme) wildtype]
D_Fructose_1,6_bisphosphate -> Glycerone_phosphate + D_Glyceraldehyde_3_phosphate |
(( (V * A) ) / (Km + A)) |
|
buffer: 0.06 M Tris-HCl, 0.28 mM NADH, 20 µg glyceraldehyde-3-phosphate dehydrogenase/triosephosphate isomerase
PH: 7.5
Temperature: 36 |
uniprot:P05062 [fructose-bisphosphate aldolase(Enzyme) wildtype isoenzyme B]
D_Fructose_1_phosphate -> D_Glyceraldehyde + Glycerone_phosphate |
(( (Vmax * A) ) / (Km + A)) |
|
buffer: 100 mM triethanolamine
PH: 7.4
Temperature: 36 |
uniprot:P05062 [fructose-bisphosphate aldolase(Enzyme) wildtype isoenzyme B]
D_Fructose_1_phosphate -> Glycerone_phosphate + D_Glyceraldehyde |
(( (Vmax * A) ) / (Km + A)) |
|
buffer: 100 mM triethanolamine
PH: 7.4
Temperature: 36 |
uniprot:P05062 [fructose-bisphosphate aldolase(Enzyme) wildtype isoenzyme B]
D_Fructose_1_phosphate -> D_Glyceraldehyde + Glycerone_phosphate |
(( (Vmax * A) ) / (Km + A)) |
|
buffer: 100 mM triethanolamine
PH: 7.4
Temperature: 36 |
uniprot:P00883 [fructose-bisphosphate aldolase(Enzyme) wildtype]
D_Fructose_1,6_bisphosphate -> Glycerone_phosphate + D_Glyceraldehyde_3_phosphate |
(( (Vmax * A) ) / (Km + A)) |
|
buffer: 0.01 M Tris/HCl
PH: 8
Temperature: 37 |
- [fructose-bisphosphate aldolase(Enzyme) wildtype]
D_Fructose_1,6_bisphosphate -> D_Glyceraldehyde_3_phosphate + Glycerone_phosphate |
(( (Vmax * A) ) / (Km + A)) |
|
buffer: 0.01 M Tris/HCl
PH: 8
Temperature: 37 |
uniprot:P05062 [fructose-bisphosphate aldolase(Enzyme) mutant aldolase B R303W]
D_Fructose_1,6_bisphosphate -> Glycerone_phosphate + D_Glyceraldehyde_3_phosphate |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 50 mM Tris-acetate, 0.15 mM NADH, 10 mM EDTA, 100 mg/ml bovine serum albumin, 2 mg/ml alpha-glycerophosphate dehydrogenase/triose phosphate isomerase
PH: 7.6
Temperature: 22 |
uniprot:P05062 [fructose-bisphosphate aldolase(Enzyme) mutant aldolase B L256P]
D_Fructose_1,6_bisphosphate -> D_Glyceraldehyde_3_phosphate + Glycerone_phosphate |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 50 mM Tris-acetate, 0.15 mM NADH, 10 mM EDTA, 100 mg/ml bovine serum albumin, 2 mg/ml alpha-glycerophosphate dehydrogenase/triose phosphate isomerase
PH: 7.6
Temperature: 22 |
uniprot:P05062 [fructose-bisphosphate aldolase(Enzyme) wildtype aldolase B]
D_Fructose_1,6_bisphosphate -> Glycerone_phosphate + D_Glyceraldehyde_3_phosphate |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 50 mM Tris-acetate, 0.15 mM NADH, 10 mM EDTA, 100 mg/ml bovine serum albumin, 2 mg/ml alpha-glycerophosphate dehydrogenase/triose phosphate isomerase
PH: 7.6
Temperature: 22 |
uniprot:P05062 [fructose-bisphosphate aldolase(Enzyme) mutant aldolase B A337V]
D_Fructose_1,6_bisphosphate -> Glycerone_phosphate + D_Glyceraldehyde_3_phosphate |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 50 mM Tris-acetate, 0.15 mM NADH, 10 mM EDTA, 100 mg/ml bovine serum albumin, 2 mg/ml alpha-glycerophosphate dehydrogenase/triose phosphate isomerase
PH: 7.6
Temperature: 22 |
uniprot:P05062 [fructose-bisphosphate aldolase(Enzyme) mutant aldolase B A149P]
D_Fructose_1,6_bisphosphate -> Glycerone_phosphate + D_Glyceraldehyde_3_phosphate |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 50 mM Tris-acetate, 0.15 mM NADH, 10 mM EDTA, 100 mg/ml bovine serum albumin, 2 mg/ml alpha-glycerophosphate dehydrogenase/triose phosphate isomerase
PH: 7.6
Temperature: 22 |
uniprot:P05062 [fructose-bisphosphate aldolase(Enzyme) mutant aldolase B W147R]
D_Fructose_1,6_bisphosphate -> Glycerone_phosphate + D_Glyceraldehyde_3_phosphate |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 50 mM Tris-acetate, 0.15 mM NADH, 10 mM EDTA, 100 mg/ml bovine serum albumin, 2 mg/ml alpha-glycerophosphate dehydrogenase/triose phosphate isomerase
PH: 7.6
Temperature: 22 |
uniprot:P05062 [fructose-bisphosphate aldolase(Enzyme) mutant aldolase B W147R]
D_Fructose_1_phosphate -> Glycerone_phosphate + D_Glyceraldehyde |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 50 mM Tris-acetate, 0.15 mM NADH, 10 mM EDTA, 100 mg/ml bovine serum albumin, 2 mg/ml alpha-glycerophosphate dehydrogenase/triose phosphate isomerase
PH: 7.6
Temperature: 22 |
uniprot:P05062 [fructose-bisphosphate aldolase(Enzyme) wildtype aldolase B]
D_Fructose_1_phosphate -> D_Glyceraldehyde + Glycerone_phosphate |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 50 mM Tris-acetate, 0.15 mM NADH, 10 mM EDTA, 100 mg/ml bovine serum albumin, 2 mg/ml alpha-glycerophosphate dehydrogenase/triose phosphate isomerase
PH: 7.6
Temperature: 22 |
uniprot:P05062 [fructose-bisphosphate aldolase(Enzyme) mutant aldolase B A149P]
D_Fructose_1_phosphate -> D_Glyceraldehyde + Glycerone_phosphate |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 50 mM Tris-acetate, 0.15 mM NADH, 10 mM EDTA, 100 mg/ml bovine serum albumin, 2 mg/ml alpha-glycerophosphate dehydrogenase/triose phosphate isomerase
PH: 7.6
Temperature: 22 |
uniprot:P05062 [fructose-bisphosphate aldolase(Enzyme) mutant aldolase B A337V]
D_Fructose_1_phosphate -> Glycerone_phosphate + D_Glyceraldehyde |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 50 mM Tris-acetate, 0.15 mM NADH, 10 mM EDTA, 100 mg/ml bovine serum albumin, 2 mg/ml alpha-glycerophosphate dehydrogenase/triose phosphate isomerase
PH: 7.6
Temperature: 22 |
uniprot:P05062 [fructose-bisphosphate aldolase(Enzyme) mutant aldolase B L256P]
D_Fructose_1_phosphate -> D_Glyceraldehyde + Glycerone_phosphate |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 50 mM Tris-acetate, 0.15 mM NADH, 10 mM EDTA, 100 mg/ml bovine serum albumin, 2 mg/ml alpha-glycerophosphate dehydrogenase/triose phosphate isomerase
PH: 7.6
Temperature: 22 |
- [fructose-bisphosphate aldolase(Enzyme) wildtype]
beta_D_Fructose_1,6_bisphosphate -> Glycerone_phosphate + D_Glyceraldehyde_3_phosphate |
(( (V * S) ) / (Km + S)) |
|
buffer: 10 mM K2HPO4/KH2PO4, 20 mM imidazole-HCl, 150 mM KCl, 5 mM MgCl2
PH: 7.2
Temperature: 38 |
uniprot:P16096 [fructose-bisphosphate aldolase(Enzyme) wildtype]
D_Fructose_1,6_bisphosphate -> Glycerone_phosphate + D_Glyceraldehyde_3_phosphate |
(( (Vmax * A) ) / (KmA + A)) |
|
buffer: 50 mM Tris-HCl, 4.5 mM MgCl2, 1 mM EDTA, 1 unit/ml triosephosphate isomerase, 1 unit/ml glycerol-3-phosphate dehydrogenase, 250 µM NADH
PH: 7.5
Temperature: 20 |
uniprot:P29356 [fructose-bisphosphate aldolase(Enzyme) wildtype]
D_Fructose_1,6_bisphosphate -> Glycerone_phosphate + D_Glyceraldehyde_3_phosphate |
(( (Vmax * A) ) / (KmA + A)) |
|
buffer: 50 mM Tris-HCl, 4.5 mM MgCl2, 1 mM EDTA, 1 unit/ml triosephosphate isomerase, 1 unit/ml glycerol-3-phosphate dehydrogenase, 250 µM NADH
PH: 7.5
Temperature: 20 |
- [fructose-bisphosphate aldolase(Enzyme) wildtype]
D_Fructose_1,6_bisphosphate -> D_Glyceraldehyde_3_phosphate + Glycerone_phosphate |
(( (Vmax * A) ) / (KmA + A)) |
|
buffer: 50 mM Tris-HCl, 4.5 mM MgCl2, 1 mM EDTA, 1 unit/ml triosephosphate isomerase, 1 unit/ml glycerol-3-phosphate dehydrogenase, 250 µM NADH
PH: 7.5
Temperature: 20 |
uniprot:Q8VWM9 [fructose-bisphosphate aldolase(Enzyme) wildtype]
D_Fructose_1,6_bisphosphate -> D_Glyceraldehyde_3_phosphate + Glycerone_phosphate |
(( (Vmax * A) ) / (KmA + A)) |
|
buffer: 50 mM Tris-HCl, 4.5 mM MgCl2, 1 mM EDTA, 1 unit/ml triosephosphate isomerase, 1 unit/ml glycerol-3-phosphate dehydrogenase, 250 µM NADH
PH: 7.5
Temperature: 20 |
- [fructose-bisphosphate aldolase(Enzyme) wildtype]
D_Fructose_1,6_bisphosphate -> Glycerone_phosphate + D_Glyceraldehyde_3_phosphate |
(( (Vmax * A) ) / (KmA + A)) |
|
buffer: 50 mM Tris-HCl, 4.5 mM MgCl2, 1 mM EDTA, 1 unit/ml triosephosphate isomerase, 1 unit/ml glycerol-3-phosphate dehydrogenase, 250 µM NADH
PH: 7.5
Temperature: 20 |
uniprot:Q8VWM9 [fructose-bisphosphate aldolase(Enzyme) wildtype]
D_Fructose_1,6_bisphosphate -> D_Glyceraldehyde_3_phosphate + Glycerone_phosphate |
(( (Vmax * A) ) / (KmA + A)) |
|
buffer: 50 mM Tris-HCl, 4.5 mM MgCl2, 1 mM EDTA, 1 unit/ml triosephosphate isomerase, 1 unit/ml glycerol-3-phosphate dehydrogenase, 250 µM NADH
PH: 7.5
Temperature: 20 |
uniprot:P08440 [fructose-bisphosphate aldolase(Enzyme) wildtype]
D_Fructose_1,6_bisphosphate -> D_Glyceraldehyde_3_phosphate + Glycerone_phosphate |
(( (Vmax * A) ) / (KmA + A)) |
|
buffer: 50 mM Tris-HCl, 4.5 mM MgCl2, 1 mM EDTA, 1 unit/ml triosephosphate isomerase, 1 unit/ml glycerol-3-phosphate dehydrogenase, 250 µM NADH
PH: 7.5
Temperature: 20 |
uniprot:P08440 [fructose-bisphosphate aldolase(Enzyme) wildtype]
D_Fructose_1,6_bisphosphate -> Glycerone_phosphate + D_Glyceraldehyde_3_phosphate |
(( (Vmax * A) ) / (KmA + A)) |
|
buffer: 50 mM Tris-HCl, 4.5 mM MgCl2, 1 mM EDTA, 1 unit/ml triosephosphate isomerase, 1 unit/ml glycerol-3-phosphate dehydrogenase, 250 µM NADH
PH: 7.5
Temperature: 20 |
uniprot:P16096 [fructose-bisphosphate aldolase(Enzyme) wildtype]
D_Fructose_1_phosphate -> D_Glyceraldehyde + Glycerone_phosphate |
(( (Vmax * A) ) / (KmA + A)) |
|
buffer: 50 mM Tris-HCl, 4.5 mM MgCl2, 1 mM EDTA, 1 unit/ml triosephosphate isomerase, 1 unit/ml glycerol-3-phosphate dehydrogenase, 250 µM NADH
PH: 7.5
Temperature: 20 |
- [fructose-bisphosphate aldolase(Enzyme) wildtype]
D_Fructose_1_phosphate -> Glycerone_phosphate + D_Glyceraldehyde |
(( (Vmax * A) ) / (KmA + A)) |
|
buffer: 50 mM Tris-HCl, 4.5 mM MgCl2, 1 mM EDTA, 1 unit/ml triosephosphate isomerase, 1 unit/ml glycerol-3-phosphate dehydrogenase, 250 µM NADH
PH: 7.5
Temperature: 20 |
uniprot:P29356 [fructose-bisphosphate aldolase(Enzyme) wildtype]
D_Fructose_1_phosphate -> Glycerone_phosphate + D_Glyceraldehyde |
(( (Vmax * A) ) / (KmA + A)) |
|
buffer: 50 mM Tris-HCl, 4.5 mM MgCl2, 1 mM EDTA, 1 unit/ml triosephosphate isomerase, 1 unit/ml glycerol-3-phosphate dehydrogenase, 250 µM NADH
PH: 7.5
Temperature: 20 |
- [fructose-bisphosphate aldolase(Enzyme) wildtype]
D_Fructose_1_phosphate -> D_Glyceraldehyde + Glycerone_phosphate |
(( (Vmax * A) ) / (KmA + A)) |
|
buffer: 50 mM Tris-HCl, 4.5 mM MgCl2, 1 mM EDTA, 1 unit/ml triosephosphate isomerase, 1 unit/ml glycerol-3-phosphate dehydrogenase, 250 µM NADH
PH: 7.5
Temperature: 20 |
uniprot:Q8VWM9 [fructose-bisphosphate aldolase(Enzyme) wildtype]
D_Fructose_1_phosphate -> Glycerone_phosphate + D_Glyceraldehyde |
(( (Vmax * A) ) / (KmA + A)) |
|
buffer: 50 mM Tris-HCl, 4.5 mM MgCl2, 1 mM EDTA, 1 unit/ml triosephosphate isomerase, 1 unit/ml glycerol-3-phosphate dehydrogenase, 250 µM NADH
PH: 7.5
Temperature: 20 |
uniprot:Q8VWM9 [fructose-bisphosphate aldolase(Enzyme) wildtype]
D_Fructose_1_phosphate -> D_Glyceraldehyde + Glycerone_phosphate |
(( (Vmax * A) ) / (KmA + A)) |
|
buffer: 50 mM Tris-HCl, 4.5 mM MgCl2, 1 mM EDTA, 1 unit/ml triosephosphate isomerase, 1 unit/ml glycerol-3-phosphate dehydrogenase, 250 µM NADH
PH: 7.5
Temperature: 20 |
uniprot:P08440 [fructose-bisphosphate aldolase(Enzyme) wildtype]
D_Fructose_1_phosphate -> D_Glyceraldehyde + Glycerone_phosphate |
(( (Vmax * A) ) / (KmA + A)) |
|
buffer: 50 mM Tris-HCl, 4.5 mM MgCl2, 1 mM EDTA, 1 unit/ml triosephosphate isomerase, 1 unit/ml glycerol-3-phosphate dehydrogenase, 250 µM NADH
PH: 7.5
Temperature: 20 |
uniprot:P08440 [fructose-bisphosphate aldolase(Enzyme) wildtype]
D_Fructose_1_phosphate -> Glycerone_phosphate + D_Glyceraldehyde |
(( (Vmax * A) ) / (KmA + A)) |
|
buffer: 50 mM Tris-HCl, 4.5 mM MgCl2, 1 mM EDTA, 1 unit/ml triosephosphate isomerase, 1 unit/ml glycerol-3-phosphate dehydrogenase, 250 µM NADH
PH: 7.5
Temperature: 20 |
uniprot:P05062 [fructose-bisphosphate aldolase(Enzyme) wildtype]
Glycerone_phosphate + D_Glyceraldehyde_3_phosphate -> D_Fructose_1,6_bisphosphate |
(( (( (kcat * E) ) * S) ) / (Km + S)) |
|
buffer: 20 mM Tris-HCl, 0.1 mg/ml BSA
PH: 7.4
Temperature: 30 |
uniprot:P05062 [fructose-bisphosphate aldolase(Enzyme) wildtype]
Glycerone_phosphate + D_Glyceraldehyde_3_phosphate -> D_Fructose_1,6_bisphosphate |
(( (( (kcat * E) ) * S) ) / (Km + S)) |
|
buffer: 20 mM Tris-HCl, 0.1 mg/ml BSA
PH: 7.4
Temperature: 10 |
uniprot:P05062 [fructose-bisphosphate aldolase(Enzyme) mutant AP]
D_Glyceraldehyde_3_phosphate + Glycerone_phosphate -> D_Fructose_1,6_bisphosphate |
(( (( (kcat * E) ) * S) ) / (Km + S)) |
|
buffer: 20 mM Tris-HCl, 0.1 mg/ml BSA
PH: 7.4
Temperature: 10 |
uniprot:P05062 [fructose-bisphosphate aldolase(Enzyme) wildtype]
D_Glyceraldehyde_3_phosphate + Glycerone_phosphate -> D_Fructose_1,6_bisphosphate |
(( (( (kcat * E) ) * S) ) / (Km + S)) |
|
buffer: 20 mM Tris-HCl, 0.1 mg/ml BSA
PH: 7.4
Temperature: 10 |
uniprot:P05062 [fructose-bisphosphate aldolase(Enzyme) wildtype]
Glycerone_phosphate + D_Glyceraldehyde_3_phosphate -> D_Fructose_1,6_bisphosphate |
(( (( (kcat * E) ) * S) ) / (Km + S)) |
|
buffer: 20 mM Tris-HCl, 0.1 mg/ml BSA
PH: 7.4
Temperature: 30 |
uniprot:P05062 [fructose-bisphosphate aldolase(Enzyme) mutant AP]
D_Glyceraldehyde_3_phosphate + Glycerone_phosphate -> D_Fructose_1,6_bisphosphate |
(( (( (kcat * E) ) * S) ) / (Km + S)) |
|
buffer: 20 mM Tris-HCl, 0.1 mg/ml BSA
PH: 7.4
Temperature: 30 |
uniprot:P05062 [fructose-bisphosphate aldolase(Enzyme) mutant AP]
Glycerone_phosphate + D_Glyceraldehyde_3_phosphate -> D_Fructose_1,6_bisphosphate |
(( (( (kcat * E) ) * S) ) / (Km + S)) |
|
buffer: 20 mM Tris-HCl, 0.1 mg/ml BSA
PH: 7.4
Temperature: 30 |
uniprot:P05062 [fructose-bisphosphate aldolase(Enzyme) mutant AP]
D_Glyceraldehyde_3_phosphate + Glycerone_phosphate -> D_Fructose_1,6_bisphosphate |
(( (( (kcat * E) ) * S) ) / (Km + S)) |
|
buffer: 20 mM Tris-HCl, 0.1 mg/ml BSA
PH: 7.4
Temperature: 10 |
uniprot:P05062 [fructose-bisphosphate aldolase(Enzyme) wildtype isoenzyme ALDOB]
D_Fructose_1,6_bisphosphate -> Glycerone_phosphate + D_Glyceraldehyde_3_phosphate |
(( (Vmax * A) ) / (Km + A)) |
|
buffer: 33 mM Tris-HCl
PH: 7.4
Temperature: 37 |
uniprot:P05062 [fructose-bisphosphate aldolase(Enzyme) wildtype isoenzyme ALDOB]
D_Fructose_1_phosphate -> D_Glyceraldehyde + Glycerone_phosphate |
(( (Vmax * A) ) / (Km + A)) |
|
buffer: 33 mM Tris-HCl
PH: 7.4
Temperature: 37 |
uniprot:P05062 [fructose-bisphosphate aldolase(Enzyme) wildtype isoenzyme ALDOB]
D_Xylulose_1_phosphate -> Glycerone_phosphate + Glycolaldehyde |
(( (Vmax * A) ) / (Km + A)) |
|
buffer: 33 mM Tris-HCl
PH: 7.4
Temperature: 37 |
uniprot:A2RN46 [fructose-bisphosphate aldolase(Enzyme) wildtype]
D_Fructose_1,6_bisphosphate -> Glycerone_phosphate + D_Glyceraldehyde_3_phosphate |
(( (Vmax * A) ) / (Km + A)) |
|
buffer: 50 mM triethanolamine-HCl, 0.25 mM NADH, 1.2 U/ml alpha-glycerolphosphate, 11.5 U/ml triose phosphate isomerase
PH: 7.8
Temperature: 25 |
- [fructose-bisphosphate aldolase(Enzyme) wildtype peak 1]
D_Fructose_1,6_bisphosphate -> Glycerone_phosphate + D_Glyceraldehyde_3_phosphate |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 50 mM Tris-HCl, 4.5 mM MgCl2, 1 mM EDTA, 1 U/ml Triosephosphate isomerase, 1 U/ml Glycerol 3-phosphate dehydrogenase, 210 µM NADH
PH: 7.5
Temperature: 22 |
- [fructose-bisphosphate aldolase(Enzyme) wildtype peak 2]
D_Fructose_1,6_bisphosphate -> Glycerone_phosphate + D_Glyceraldehyde_3_phosphate |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 50 mM Tris-HCl, 4.5 mM MgCl2, 1 mM EDTA, 1 U/ml Triosephosphate isomerase, 1 U/ml Glycerol 3-phosphate dehydrogenase, 210 µM NADH
PH: 7.5
Temperature: 22 |
uniprot:P00883 [fructose-bisphosphate aldolase(Enzyme) wildtype]
D_Fructose_1,6_bisphosphate -> Glycerone_phosphate + D_Glyceraldehyde_3_phosphate |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 50 mM Pipes, 100 mM KCl, 5 mM magnesium acetate, 0.5 mM triose-phosphate isomerase, 1 mM glycerol-3-phosphate dehydrogenase
PH: 7.5
Temperature: 25 |
uniprot:P0A991 [Citrate]
D_Fructose_1,6_bisphosphate -> D_Glyceraldehyde_3_phosphate + Glycerone_phosphate |
(( (( (E * kcat) ) * S) ) / (Km + S)) |
|
buffer: 50 mM Tris-HCl, 20 mM NADH, 2 µl of a 10 mg/ml mixture of glycerol phosphate dehydrogenase/triose phosphate isomerase
PH: 8
Temperature: 30 |
uniprot:P0A991 [fructose-bisphosphate aldolase(Enzyme) wildtype]
D_Fructose_1,6_bisphosphate -> D_Glyceraldehyde_3_phosphate + Glycerone_phosphate |
(( (( (E * kcat) ) * S) ) / (Km + S)) |
|
buffer: 50 mM Tris-HCl, 20 mM NADH, 2 µl of a 10 mg/ml mixture of glycerol phosphate dehydrogenase/triose phosphate isomerase
PH: 8
Temperature: 30 |
- [fructose-bisphosphate aldolase(Enzyme) wildtype]
D_Fructose_1,6_bisphosphate -> D_Glyceraldehyde_3_phosphate + Glycerone_phosphate |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 50 mM Tris-HCl, 0.1 mM NADH, 10 U/ml triosephosphate isomerase, 1 U/ml glycerol 3-phosphate dehydrogenase
PH: 7.5
Temperature: 30 |
- [fructose-bisphosphate aldolase(Enzyme) wildtype]
Sedoheptulose_1,7_bisphosphate -> D_Erythrose_4_phosphate + Glycerone_phosphate |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 50 mM Tris-HCl, 0.1 mM NADH, 10 U/ml triosephosphate isomerase, 1 U/ml glycerol 3-phosphate dehydrogenase
PH: 7.5
Temperature: 30 |
- [ATP]
D_Fructose_1,6_bisphosphate -> D_Glyceraldehyde_3_phosphate + Glycerone_phosphate |
(( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) |
|
buffer: 50 mM Tris-HCl, 0.1 mM NADH, 10 U/ml triosephosphate isomerase, 1 U/ml glycerol 3-phosphate dehydrogenase
PH: 7.5
Temperature: 30 |
uniprot:P0A991 [Citrate]
D_Fructose_1,6_bisphosphate -> Glycerone_phosphate + D_Glyceraldehyde_3_phosphate |
(( (( (E * kcat) ) * S) ) / (Km + S)) |
|
buffer: 50 mM Tris-HCl, 20 mM NADH, 2 µl of a 10 mg/ml mixture of glycerol phosphate dehydrogenase/triose phosphate isomerase
PH: 8
Temperature: 30 |
uniprot:P0A991 [Citrate]
D_Fructose_1,6_bisphosphate -> D_Glyceraldehyde_3_phosphate + Glycerone_phosphate |
(( (( (E * kcat) ) * S) ) / (Km + S)) |
|
buffer: 50 mM Tris-HCl, 20 mM NADH, 2 µl of a 10 mg/ml mixture of glycerol phosphate dehydrogenase/triose phosphate isomerase
PH: 8
Temperature: 30 |
uniprot:P0A991 [fructose-bisphosphate aldolase(Enzyme) mutant K239A]
D_Fructose_1,6_bisphosphate -> Glycerone_phosphate + D_Glyceraldehyde_3_phosphate |
(( (( (E * kcat) ) * S) ) / (Km + S)) |
|
buffer: 50 mM Tris-HCl, 20 mM NADH, 2 µl of a 10 mg/ml mixture of glycerol phosphate dehydrogenase/triose phosphate isomerase
PH: 8
Temperature: 30 |
uniprot:P0A991 [fructose-bisphosphate aldolase(Enzyme) wildtype]
D_Fructose_1_phosphate -> D_Glyceraldehyde + Glycerone_phosphate |
(( (( (E * kcat) ) * S) ) / (Km + S)) |
|
buffer: 50 mM Tris-HCl, 20 mM NADH, 2 µl of a 10 mg/ml mixture of glycerol phosphate dehydrogenase/triose phosphate isomerase
PH: 8
Temperature: 30 |
uniprot:P0A991 [fructose-bisphosphate aldolase(Enzyme) mutant K239A]
D_Fructose_1_phosphate -> Glycerone_phosphate + D_Glyceraldehyde |
(( (( (E * kcat) ) * S) ) / (Km + S)) |
|
buffer: 50 mM Tris-HCl, 20 mM NADH, 2 µl of a 10 mg/ml mixture of glycerol phosphate dehydrogenase/triose phosphate isomerase
PH: 8
Temperature: 30 |
- [fructose-bisphosphate aldolase(Enzyme) wildtype]
D_Fructose_1,6_bisphosphate -> D_Glyceraldehyde_3_phosphate + Glycerone_phosphate |
(( (Vmax * A) ) / (Km + A)) |
|
buffer: 100 mM Tris/HCl, 1.7 mM NAD, 0.8 mM Na2HAsO4, 33 U glyceraldehyde-3-phosphate dehydrogenase
PH: 7.6
Temperature: 25 |
uniprot:P0AB71 [fructose-bisphosphate aldolase(Enzyme) mutant E182A]
D_Fructose_1,6_bisphosphate -> D_Glyceraldehyde_3_phosphate + Glycerone_phosphate |
(( (( (E * kcat) ) * A) ) / (Km + A)) |
|
buffer: 50 mM Tris-HCl, 20 mM NADH, Glycerol phosphate dehydrogenase, Triosephosphate isomerase
PH: 8
Temperature: 30 |
uniprot:P0AB71 [fructose-bisphosphate aldolase(Enzyme) mutant E174A]
D_Fructose_1,6_bisphosphate -> D_Glyceraldehyde_3_phosphate + Glycerone_phosphate |
(( (( (E * kcat) ) * A) ) / (Km + A)) |
|
buffer: 50 mM Tris-HCl, 20 mM NADH, Glycerol phosphate dehydrogenase, Triosephosphate isomerase
PH: 8
Temperature: 30 |
uniprot:P0AB71 [fructose-bisphosphate aldolase(Enzyme) wildtype]
D_Fructose_1,6_bisphosphate -> D_Glyceraldehyde_3_phosphate + Glycerone_phosphate |
(( (( (E * kcat) ) * A) ) / (Km + A)) |
|
buffer: 50 mM Tris-HCl, 20 mM NADH, Glycerol phosphate dehydrogenase, Triosephosphate isomerase
PH: 8
Temperature: 30 |
uniprot:P0AB71 [fructose-bisphosphate aldolase(Enzyme) mutant E181A]
D_Fructose_1,6_bisphosphate -> D_Glyceraldehyde_3_phosphate + Glycerone_phosphate |
(( (( (E * kcat) ) * A) ) / (Km + A)) |
|
buffer: 50 mM Tris-HCl, 20 mM NADH, Glycerol phosphate dehydrogenase, Triosephosphate isomerase
PH: 8
Temperature: 30 |
uniprot:P0AB71 [fructose-bisphosphate aldolase(Enzyme) mutant LOOP (G176A, G179A, G180A, G184A)]
D_Fructose_1,6_bisphosphate -> D_Glyceraldehyde_3_phosphate + Glycerone_phosphate |
(( (( (E * kcat) ) * A) ) / (Km + A)) |
|
buffer: 50 mM Tris-HCl, 20 mM NADH, Glycerol phosphate dehydrogenase, Triosephosphate isomerase
PH: 8
Temperature: 30 |
uniprot:P0AB71 [fructose-bisphosphate aldolase(Enzyme) mutant E174A]
D_Fructose_1,6_bisphosphate -> D_Glyceraldehyde_3_phosphate + Glycerone_phosphate |
(( (( (E * kcat) ) * A) ) / (Km + A)) |
|
buffer: 50 mM Tris-HCl, 0.1 M Potassium acetate, 2 mM Potassium hexacyanoferrate(III)
PH: 8
Temperature: 30 |
uniprot:P0AB71 [fructose-bisphosphate aldolase(Enzyme) mutant E181A]
D_Fructose_1,6_bisphosphate -> Glycerone_phosphate + D_Glyceraldehyde_3_phosphate |
(( (( (E * kcat) ) * A) ) / (Km + A)) |
|
buffer: 50 mM Tris-HCl, 0.1 M Potassium acetate, 2 mM Potassium hexacyanoferrate(III)
PH: 8
Temperature: 30 |
uniprot:P0AB71 [fructose-bisphosphate aldolase(Enzyme) mutant E182A]
D_Fructose_1,6_bisphosphate -> D_Glyceraldehyde_3_phosphate + Glycerone_phosphate |
(( (( (E * kcat) ) * A) ) / (Km + A)) |
|
buffer: 50 mM Tris-HCl, 0.1 M Potassium acetate, 2 mM Potassium hexacyanoferrate(III)
PH: 8
Temperature: 30 |
uniprot:P0AB71 [fructose-bisphosphate aldolase(Enzyme) wildtype]
D_Fructose_1,6_bisphosphate -> D_Glyceraldehyde_3_phosphate + Glycerone_phosphate |
(( (( (E * kcat) ) * A) ) / (Km + A)) |
|
buffer: 50 mM Tris-HCl, 0.1 M Potassium acetate, 2 mM Potassium hexacyanoferrate(III)
PH: 8
Temperature: 30 |
uniprot:P0AB71 [fructose-bisphosphate aldolase(Enzyme) mutant LOOP (G176A, G179A, G180A, G184A)]
D_Fructose_1,6_bisphosphate -> Glycerone_phosphate + D_Glyceraldehyde_3_phosphate |
(( (( (E * kcat) ) * A) ) / (Km + A)) |
|
buffer: 50 mM Tris-HCl, 0.1 M Potassium acetate, 2 mM Potassium hexacyanoferrate(III)
PH: 8
Temperature: 30 |
uniprot:P0AB71 [fructose-bisphosphate aldolase(Enzyme) mutant LOOP (G176A, G179A, G180A, G184A)]
Glycerone_phosphate + D_Glyceraldehyde_3_phosphate -> D_Fructose_1,6_bisphosphate |
(( (( (E * kcat) ) * A) ) / (Km + A)) |
|
buffer: 50 mM Tris-HCl, 0.1 M Potassium acetate, 2 mM Potassium hexacyanoferrate(III)
PH: 8
Temperature: 30 |
uniprot:P0AB71 [fructose-bisphosphate aldolase(Enzyme) mutant E174A]
Glycerone_phosphate + D_Glyceraldehyde_3_phosphate -> D_Fructose_1,6_bisphosphate |
(( (( (E * kcat) ) * A) ) / (Km + A)) |
|
buffer: 50 mM Tris-HCl, 0.1 M Potassium acetate, 2 mM Potassium hexacyanoferrate(III)
PH: 8
Temperature: 30 |
uniprot:P0AB71 [fructose-bisphosphate aldolase(Enzyme) wildtype]
D_Glyceraldehyde_3_phosphate + Glycerone_phosphate -> D_Fructose_1,6_bisphosphate |
(( (( (E * kcat) ) * A) ) / (Km + A)) |
|
buffer: 50 mM Tris-HCl, 0.1 M Potassium acetate, 2 mM Potassium hexacyanoferrate(III)
PH: 8
Temperature: 30 |
uniprot:P0AB71 [fructose-bisphosphate aldolase(Enzyme) mutant E181A]
Glycerone_phosphate + D_Glyceraldehyde_3_phosphate -> D_Fructose_1,6_bisphosphate |
(( (( (E * kcat) ) * A) ) / (Km + A)) |
|
buffer: 50 mM Tris-HCl, 0.1 M Potassium acetate, 2 mM Potassium hexacyanoferrate(III)
PH: 8
Temperature: 30 |
uniprot:P0AB71 [fructose-bisphosphate aldolase(Enzyme) mutant E182A]
Glycerone_phosphate + D_Glyceraldehyde_3_phosphate -> D_Fructose_1,6_bisphosphate |
(( (( (E * kcat) ) * A) ) / (Km + A)) |
|
buffer: 50 mM Tris-HCl, 0.1 M Potassium acetate, 2 mM Potassium hexacyanoferrate(III)
PH: 8
Temperature: 30 |
uniprot:C4LXD7 [fructose-bisphosphate aldolase(Enzyme) wildtype His-tagged]
D_Fructose_1,6_bisphosphate -> Glycerone_phosphate + D_Glyceraldehyde_3_phosphate |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 50 mM imidazole, 10 mM Acetic acid/Mes/Tris, 1.5-3 U glycerol-3-phosphate dehydrogenase, 4.5-9 U triosephosphate isomerase
PH: 7
Temperature: 37 |
uniprot:C4LXD7 [Co2+]
D_Fructose_1,6_bisphosphate -> D_Glyceraldehyde_3_phosphate + Glycerone_phosphate |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 50 mM imidazole, 10 mM Acetic acid/Mes/Tris, 1.5-3 U glycerol-3-phosphate dehydrogenase, 4.5-9 U triosephosphate isomerase
PH: 6
Temperature: 37 |
uniprot:C4LXD7 [fructose-bisphosphate aldolase(Enzyme) wildtype His-tagged]
D_Fructose_1,6_bisphosphate -> D_Glyceraldehyde_3_phosphate + Glycerone_phosphate |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 50 mM imidazole, 10 mM Acetic acid/Mes/Tris, 1.5-3 U glycerol-3-phosphate dehydrogenase, 4.5-9 U triosephosphate isomerase
PH: 6
Temperature: 37 |
- [fructose-bisphosphate aldolase(Enzyme) wildtype His-tagged]
Glycerone_phosphate + D_Glyceraldehyde_3_phosphate -> D_Fructose_1,6_bisphosphate |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 50 mM imidazole, 10 mM Acetic acid/Mes/Tris, 15 mM MgCl2, 1 mM NADP+, 0.5 mM CoCl2, 2-4 U glucose 6-phosphate dehydrogenase, 6-8 U hexose 6-phosphate isomerase, 0.5-1 U phosphofructokinase, 10 mM Pi, 0.05-3.0 mM dihydroxyacetone phosphate
PH: 6
Temperature: 37 |
- [fructose-bisphosphate aldolase(Enzyme) wildtype His-tagged]
D_Glyceraldehyde_3_phosphate + Glycerone_phosphate -> D_Fructose_1,6_bisphosphate |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 50 mM imidazole, 10 mM Acetic acid/Mes/Tris, 15 mM MgCl2,1 mM NADP+, 0.5 mM CoCl2, 2-4 U glucose 6-phosphate dehydrogenase, 6-8 U hexose 6-phosphate isomerase, 0.5-1 U phosphofructokinase, 10 mM Pi, 0.05-3.0 mM dihydroxyacetone phosphate
PH: 7
Temperature: 37 |
- [fructose-bisphosphate aldolase(Enzyme) wildtype]
D_Fructose_1,6_bisphosphate -> D_Glyceraldehyde_3_phosphate + Glycerone_phosphate |
(( (( (E * kcat) ) * S) ) / (Km + S)) |
|
buffer: 20 mM imidazole-HCl, 0.10 mM NADH, 1 U/ml triosephosphate isomerase, 2 U/ml alpha-glycerophosphate dehydrogenase
PH: 7.2
Temperature: 37 |
- [fructose-bisphosphate aldolase(Enzyme) wildtype]
D_Fructose_1,6_bisphosphate -> D_Glyceraldehyde_3_phosphate + Glycerone_phosphate |
(( (( (E * kcat) ) * S) ) / (Km + S)) |
|
buffer: 20 mM imidazole-HCl, 0.10 mM NADH, 1 U/ml triosephosphate isomerase, 2 U/ml alpha-glycerophosphate dehydrogenase
PH: 7.5
Temperature: 5 |
- [fructose-bisphosphate aldolase(Enzyme) wildtype]
D_Fructose_1,6_bisphosphate -> Glycerone_phosphate + D_Glyceraldehyde_3_phosphate |
(( (( (E * kcat) ) * S) ) / (Km + S)) |
|
buffer: 20 mM imidazole-HCl, 0.10 mM NADH, 1 U/ml triosephosphate isomerase, 2 U/ml alpha-glycerophosphate dehydrogenase
PH: 7.2
Temperature: 37 |
- [fructose-bisphosphate aldolase(Enzyme) wildtype]
D_Fructose_1,6_bisphosphate -> D_Glyceraldehyde_3_phosphate + Glycerone_phosphate |
(( (( (E * kcat) ) * S) ) / (Km + S)) |
|
buffer: 20 mM imidazole-HCl, 0.10 mM NADH, 1 U/ml triosephosphate isomerase, 2 U/ml alpha-glycerophosphate dehydrogenase
PH: 7.5
Temperature: 5 |
uniprot:C4LXD7 [fructose-bisphosphate aldolase(Enzyme) wildtype His-tagged]
Glycerone_phosphate + D_Glyceraldehyde_3_phosphate -> D_Fructose_1,6_bisphosphate |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 50 mM imidazole, 10 mM Acetic acid/Mes/Tris, 15 mM MgCl2,1 mM NADP+, 0.5 mM CoCl2, 2-4 U glucose 6-phosphate dehydrogenase, 6-8 U hexose 6-phosphate isomerase, 0.5-1 U phosphofructokinase, 10 mM Pi, 0.05-3.0 mM dihydroxyacetone phosphate
PH: 7
Temperature: 37 |
uniprot:P05064 [fructose-bisphosphate aldolase(Enzyme) wildtype]
D_Fructose_1,6_bisphosphate -> Glycerone_phosphate + D_Glyceraldehyde_3_phosphate |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 50 mM Hepes, 100 mM KCl, 10 mM NaH2PO4, 10 mM MgCl2
PH: 7.4
Temperature: 37 |
uniprot:C4LXD7 [Co2+]
D_Fructose_1,6_bisphosphate -> D_Glyceraldehyde_3_phosphate + Glycerone_phosphate |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 50 mM imidazole, 10 mM Acetic acid/Mes/Tris, 1.5-3 U glycerol-3-phosphate dehydrogenase, 4.5-9 U triosephosphate isomerase
PH: 7
Temperature: 37 |
uniprot:Q8J308 [Citrate]
D_Fructose_1,6_bisphosphate -> D_Glyceraldehyde_3_phosphate + Glycerone_phosphate |
(( (Vmax * A) ) / (Km + A)) |
|
buffer: 20 mM MES
PH: 6.5
Temperature: 70 |
uniprot:Q8J308 [Citrate]
D_Fructose_1_phosphate -> D_Glyceraldehyde + Glycerone_phosphate |
(( (Vmax * A) ) / (Km + A)) |
|
buffer: 20 mM MES
PH: 6.5
Temperature: 70 |
- [fructose-bisphosphate aldolase(Enzyme) wildtype]
D_Fructose_1,6_bisphosphate -> D_Glyceraldehyde_3_phosphate + Glycerone_phosphate |
(( (Vmax * A) ) / (Km + A)) |
|
buffer: 50 mM Tris-HCl, 0.2 mM NADH, 6.6 mM L-Glycerophosphate isomerase, 0.66 mg/ml Bovine serum albumin
PH: 7.5
Temperature: 25 |
- [fructose-bisphosphate aldolase(Enzyme) wildtype]
D_Fructose_1_phosphate -> D_Glyceraldehyde + Glycerone_phosphate |
(( (Vmax * A) ) / (Km + A)) |
|
buffer: 50 mM Tris-HCl, 0.2 mM NADH, 6.6 mM L-Glycerophosphate isomerase, 0.66 mg/ml Bovine serum albumin
PH: 7.5
Temperature: 25 |
uniprot:P00883 [fructose-bisphosphate aldolase(Enzyme) wildtype]
D_Fructose_1,6_bisphosphate -> D_Glyceraldehyde_3_phosphate + Glycerone_phosphate |
(( (( (E * kcat) ) * A) ) / (Km + A)) |
|
buffer: 0.05 M barbital sodium acetate
PH: 7.6
Temperature: 25 |
uniprot:P00883 [fructose-bisphosphate aldolase(Enzyme) wildtype]
D_Fructose_1,6_bisphosphate -> Glycerone_phosphate + D_Glyceraldehyde_3_phosphate |
(( (( (E * kcat) ) * A) ) / (Km + A)) |
|
buffer: 0.05 M barbital sodium acetate
PH: 5.5
Temperature: 25 |
uniprot:Q38B42 [fructose-bisphosphate aldolase(Enzyme) wildtype]
D_Fructose_1,6_bisphosphate -> D_Glyceraldehyde_3_phosphate + Glycerone_phosphate |
(( (( (E * kcat) ) * A) ) / (Km + A)) |
|
buffer: 0.05 M barbital sodium acetate
PH: 7.6
Temperature: 25 |
uniprot:Q38B42 [fructose-bisphosphate aldolase(Enzyme) wildtype]
D_Fructose_1,6_bisphosphate -> Glycerone_phosphate + D_Glyceraldehyde_3_phosphate |
(( (( (E * kcat) ) * A) ) / (Km + A)) |
|
buffer: 0.05 M barbital sodium acetate
PH: 5.5
Temperature: 25 |
uniprot:Q38B42 [fructose-bisphosphate aldolase(Enzyme) wildtype]
D_Fructose_1,6_bisphosphate -> D_Glyceraldehyde_3_phosphate + Glycerone_phosphate |
(( (( (E * kcat) ) * A) ) / (Km + A)) |
|
buffer: 0.05 M barbital sodium acetate
PH: 9.2
Temperature: 25 |
uniprot:P00883 [fructose-bisphosphate aldolase(Enzyme) wildtype]
D_Fructose_1,6_bisphosphate -> D_Glyceraldehyde_3_phosphate + Glycerone_phosphate |
(( (( (E * kcat) ) * A) ) / (Km + A)) |
|
buffer: 0.05 M barbital sodium acetate
PH: 9.3
Temperature: 25 |
- [fructose-bisphosphate aldolase(Enzyme) wildtype]
D_Fructose_1,6_bisphosphate -> Glycerone_phosphate + D_Glyceraldehyde_3_phosphate |
(( (( (E * kcat) ) * A) ) / (Km + A)) |
|
buffer: 0.05 M barbital sodium acetate
PH: 5.5
Temperature: 25 |
- [fructose-bisphosphate aldolase(Enzyme) wildtype]
D_Fructose_1,6_bisphosphate -> Glycerone_phosphate + D_Glyceraldehyde_3_phosphate |
(( (( (E * kcat) ) * A) ) / (Km + A)) |
|
buffer: 0.05 M barbital sodium acetate
PH: 9.3
Temperature: 25 |
- [fructose-bisphosphate aldolase(Enzyme) wildtype]
D_Fructose_1,6_bisphosphate -> D_Glyceraldehyde_3_phosphate + Glycerone_phosphate |
(( (( (E * kcat) ) * A) ) / (Km + A)) |
|
buffer: 0.05 M barbital sodium acetate
PH: 7.6
Temperature: 25 |
uniprot:P00883 [fructose-bisphosphate aldolase(Enzyme) wildtype]
D_Fructose_1_phosphate -> D_Glyceraldehyde + Glycerone_phosphate |
(( (( (E * kcat) ) * A) ) / (Km + A)) |
|
buffer: 0.05 M barbital sodium acetate
PH: 6.1
Temperature: 25 |
uniprot:P00883 [fructose-bisphosphate aldolase(Enzyme) wildtype]
D_Fructose_1_phosphate -> D_Glyceraldehyde + Glycerone_phosphate |
(( (( (E * kcat) ) * A) ) / (Km + A)) |
|
buffer: 0.05 M barbital sodium acetate
PH: 7.6
Temperature: 25 |
uniprot:Q38B42 [fructose-bisphosphate aldolase(Enzyme) wildtype]
D_Fructose_1_phosphate -> D_Glyceraldehyde + Glycerone_phosphate |
(( (( (E * kcat) ) * A) ) / (Km + A)) |
|
buffer: 0.05 M barbital sodium acetate
PH: 6.2
Temperature: 25 |
uniprot:Q38B42 [fructose-bisphosphate aldolase(Enzyme) wildtype]
D_Fructose_1_phosphate -> D_Glyceraldehyde + Glycerone_phosphate |
(( (( (E * kcat) ) * A) ) / (Km + A)) |
|
buffer: 0.05 M barbital sodium acetate
PH: 7.6
Temperature: 25 |
uniprot:Q38B42 [fructose-bisphosphate aldolase(Enzyme) wildtype]
D_Fructose_1_phosphate -> D_Glyceraldehyde + Glycerone_phosphate |
(( (( (E * kcat) ) * A) ) / (Km + A)) |
|
buffer: 0.05 M barbital sodium acetate
PH: 8.5
Temperature: 25 |
uniprot:P00883 [fructose-bisphosphate aldolase(Enzyme) wildtype]
Glycerone_phosphate + D_Glyceraldehyde_3_phosphate -> D_Fructose_1,6_bisphosphate |
(( (( (E * kcat) ) * A) ) / (Km + A)) |
|
buffer: 0.05 M triethanolamine, 1 mM EDTA, 50 mM KCl, 4 mM MgCl2, 0.2 mM NADP+, 2 µg glucose phosphate isomrase, 7 µg glucose-6-phosphate dehydrogenase, 75 µg fructose bisphosphatase, 50 µM 2-phosphoglycolate
PH: 7.6
Temperature: 25 |
uniprot:Q38B42 [fructose-bisphosphate aldolase(Enzyme) wildtype]
Glycerone_phosphate + D_Glyceraldehyde_3_phosphate -> D_Fructose_1,6_bisphosphate |
(( (( (E * kcat) ) * A) ) / (Km + A)) |
|
buffer: 0.05 M triethanolamine, 1 mM EDTA, 50 mM KCl, 4 mM MgCl2, 0.2 mM NADP+, 2 µg glucose phosphate isomrase, 7 µg glucose-6-phosphate dehydrogenase, 75 µg fructose bisphosphatase, 50 µM 2-phosphoglycolate
PH: 7.6
Temperature: 25 |
uniprot:Q38B42 [fructose-bisphosphate aldolase(Enzyme) wildtype]
Glycerone_phosphate + D_Glyceraldehyde_3_phosphate -> D_Fructose_1,6_bisphosphate |
(( (( (E * kcat) ) * A) ) / (Km + A)) |
|
buffer: 0.05 M triethanolamine, 1 mM EDTA, 50 mM KCl, 4 mM MgCl2, 0.2 mM NADP+, 2 µg glucose phosphate isomrase, 7 µg glucose-6-phosphate dehydrogenase, 75 µg fructose bisphosphatase, 50 µM 2-phosphoglycolate
PH: 7.6
Temperature: 25 |
uniprot:P00883 [fructose-bisphosphate aldolase(Enzyme) wildtype]
D_Glyceraldehyde_3_phosphate + Glycerone_phosphate -> D_Fructose_1,6_bisphosphate |
(( (( (E * kcat) ) * A) ) / (Km + A)) |
|
buffer: 0.05 M triethanolamine, 1 mM EDTA, 50 mM KCl, 4 mM MgCl2, 0.2 mM NADP+, 2 µg glucose phosphate isomrase, 7 µg glucose-6-phosphate dehydrogenase, 75 µg fructose bisphosphatase, 50 µM 2-phosphoglycolate
PH: 7.6
Temperature: 25 |
- [fructose-bisphosphate aldolase(Enzyme) wildtype]
Glycerone_phosphate + D_Glyceraldehyde_3_phosphate -> D_Fructose_1,6_bisphosphate |
(( (( (E * kcat) ) * A) ) / (Km + A)) |
|
buffer: 0.05 M triethanolamine, 1 mM EDTA, 50 mM KCl, 4 mM MgCl2, 0.2 mM NADP+, 2 µg glucose phosphate isomrase, 7 µg glucose-6-phosphate dehydrogenase, 75 µg fructose bisphosphatase, 50 µM 2-phosphoglycolate
PH: 7.6
Temperature: 25 |
uniprot:P00883 [fructose-bisphosphate aldolase(Enzyme) wildtype]
D_Fructose_1_phosphate -> D_Glyceraldehyde + Glycerone_phosphate |
(( (( (E * kcat) ) * A) ) / (Km + A)) |
|
buffer: 0.05 M barbital sodium acetate
PH: 8.7
Temperature: 25 |
- [fructose-bisphosphate aldolase(Enzyme) wildtype]
D_Fructose_1_phosphate -> Glycerone_phosphate + D_Glyceraldehyde |
(( (( (E * kcat) ) * A) ) / (Km + A)) |
|
buffer: 0.05 M barbital sodium acetate
PH: 8.6
Temperature: 25 |
- [fructose-bisphosphate aldolase(Enzyme) wildtype]
D_Fructose_1_phosphate -> Glycerone_phosphate + D_Glyceraldehyde |
(( (( (E * kcat) ) * A) ) / (Km + A)) |
|
buffer: 0.05 M barbital sodium acetate
PH: 6.1
Temperature: 25 |
- [fructose-bisphosphate aldolase(Enzyme) wildtype]
D_Fructose_1_phosphate -> D_Glyceraldehyde + Glycerone_phosphate |
(( (( (E * kcat) ) * A) ) / (Km + A)) |
|
buffer: 0.05 M barbital sodium acetate
PH: 7.6
Temperature: 25 |
- [fructose-bisphosphate aldolase(Enzyme) wildtype]
Glycerone_phosphate + D_Glyceraldehyde_3_phosphate -> D_Fructose_1,6_bisphosphate |
(( (( (E * kcat) ) * A) ) / (Km + A)) |
|
buffer: 0.05 M triethanolamine, 1 mM EDTA, 50 mM KCl, 4 mM MgCl2, 0.2 mM NADP+, 2 µg glucose phosphate isomrase, 7 µg glucose-6-phosphate dehydrogenase, 75 µg fructose bisphosphatase, 50 µM 2-phosphoglycolate
PH: 7.6
Temperature: 25 |
- [fructose-bisphosphate aldolase(Enzyme) wildtype]
D_Fructose_1,6_bisphosphate -> D_Glyceraldehyde_3_phosphate + Glycerone_phosphate |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 30 mM Triethanolamine-HCl, 0.1 mM NADH, 3 mM MgSO4, 50 mM KCL, 0.2 mM EDTA, 0.2 mg/ml BSA, 2 U/ml glycerol-3-phosphate dehydrogenase, 2 U/ml triosephosphate isomerase
PH: 7.5
Temperature: 36 |
- [fructose-bisphosphate aldolase(Enzyme) wildtype]
D_Fructose_1,6_bisphosphate -> Glycerone_phosphate + D_Glyceraldehyde_3_phosphate |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 30 mM Triethanolamine-HCl, 0.1 mM NADH, 3 mM MgSO4, 50 mM KCL, 0.2 mM EDTA, 0.2 mg/ml BSA, 2 U/ml glycerol-3-phosphate dehydrogenase, 2 U/ml triosephosphate isomerase
PH: 7.5
Temperature: 36 |
- [fructose-bisphosphate aldolase(Enzyme) wildtype]
D_Fructose_1,6_bisphosphate -> D_Glyceraldehyde_3_phosphate + Glycerone_phosphate |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 30 mM Triethanolamine-HCl, 0.1 mM NADH, 3 mM MgSO4, 50 mM KCL, 0.2 mM EDTA, 0.2 mg/ml BSA, 2 U/ml glycerol-3-phosphate dehydrogenase, 2 U/ml triosephosphate isomerase
PH: 7.5
Temperature: 36 |
uniprot:P14540 [fructose-bisphosphate aldolase(Enzyme) wildtype]
D_Fructose_1,6_bisphosphate -> D_Glyceraldehyde_3_phosphate + Glycerone_phosphate |
(( (( (kcat * Et) ) * S) ) / (Ks + S)) |
|
buffer: 100.0 mM 2-(N-morpholino)ethanesulfonic acid, 5.0 mM magnesium dichloride, 0.15 mM NADH, 0.0 mM Glycerol-3-phosphate dehydrogenase, 100.0 mM potassium chloride, 0.0 mM Triosephosphate isomerase
PH: 6.5
Temperature: 30 |
uniprot:P00883 [fructose-bisphosphate aldolase(Enzyme) wildtype]
D_Fructose_1,6_bisphosphate -> D_Glyceraldehyde_3_phosphate + Glycerone_phosphate |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 30 mM Triethanolamine-HCl, 0.1 mM NADH, 3 mM MgSO4, 50 mM KCL, 0.2 mM EDTA, 0.2 mg/ml BSA, 2 U/ml glycerol-3-phosphate dehydrogenase, 2 U/ml triosephosphate isomerase
PH: 7.5
Temperature: 36 |
- [fructose-bisphosphate aldolase(Enzyme) wildtype]
D_Fructose_1,6_bisphosphate -> D_Glyceraldehyde_3_phosphate + Glycerone_phosphate |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: *
PH: 7
Temperature: 36 |
- [fructose-bisphosphate aldolase(Enzyme) wildtype]
D_Fructose_1,6_bisphosphate -> Glycerone_phosphate + D_Glyceraldehyde_3_phosphate |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: *
PH: 7
Temperature: 36 |
- [fructose-bisphosphate aldolase(Enzyme) wildtype]
D_Fructose_1_phosphate -> Glycerone_phosphate + D_Glyceraldehyde |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: *
PH: 7
Temperature: 36 |
- [fructose-bisphosphate aldolase(Enzyme) wildtype]
D_Fructose_1_phosphate -> D_Glyceraldehyde + Glycerone_phosphate |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: *
PH: 7
Temperature: 36 |
uniprot:P58314 [fructose-bisphosphate aldolase(Enzyme) wildtype]
D_Fructose_1,6_bisphosphate -> Glycerone_phosphate + D_Glyceraldehyde_3_phosphate |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 50 mM Tris-HCl, 0.2 NADH, 4 units glycerol 3-phosphate dehydrogenase, 11 units triose-phosphate isomerase
PH: 7
Temperature: 50 |
uniprot:P58315 [fructose-bisphosphate aldolase(Enzyme) wildtype]
D_Fructose_1,6_bisphosphate -> D_Glyceraldehyde_3_phosphate + Glycerone_phosphate |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 100 mM Tris-HCl, 0.4 mM NADH, 4 units glycerol 3-phosphate dehydrogenase, 20 units triose-phosphate isomerase
PH: 7
Temperature: 50 |
uniprot:Q9RHA2 [fructose-bisphosphate aldolase(Enzyme) wildtype]
D_Fructose_1,6_bisphosphate -> D_Glyceraldehyde_3_phosphate + Glycerone_phosphate |
(( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) |
|
buffer: 50 mM Tris-HCl, 1 mM CoCl2, triose-isomerase, beta-glycerol-3-phosphate dehydrogenase
PH: 6.5
Temperature: 70 |
uniprot:Q9RHA2 [fructose-bisphosphate aldolase(Enzyme) wildtype]
D_Fructose_1,6_bisphosphate -> Glycerone_phosphate + D_Glyceraldehyde_3_phosphate |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 50 mM Tris-HCl, 1 mM CoCl2, triose-isomerase, beta-glycerol-3-phosphate dehydrogenase
PH: 6.5
Temperature: 70 |
uniprot:P58314 [fructose-bisphosphate aldolase(Enzyme) wildtype]
D_Fructose_1_phosphate -> D_Glyceraldehyde + Glycerone_phosphate |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 50 mM Tris-HCl, 0.2 NADH, 4 units glycerol 3-phosphate dehydrogenase, 11 units triose-phosphate isomerase
PH: 7
Temperature: 50 |
uniprot:P58315 [fructose-bisphosphate aldolase(Enzyme) wildtype]
D_Fructose_1_phosphate -> D_Glyceraldehyde + Glycerone_phosphate |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 100 mM Tris-HCl, 0.4 mM NADH, 4 units glycerol 3-phosphate dehydrogenase, 20 units triose-phosphate isomerase
PH: 7
Temperature: 50 |
uniprot:P00883 [fructose-bisphosphate aldolase(Enzyme) wildtype]
D_Fructose_1,6_bisphosphate -> Glycerone_phosphate + D_Glyceraldehyde_3_phosphate |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 50 mM Tris-HCl, 1 mM EDTA, 100 µg/ml glycerol dehydrogenase
PH: 7.5
Temperature: 36 |
uniprot:P00883 [fructose-bisphosphate aldolase(Enzyme) wildtype]
[(2R,3S,4S,5R)_3,4,5_trihydroxy_5_(phosphonohydroxymethyl)oxolan_2_yl]methyl_phosphate -> 4_Hydroxy_3_oxobutylphosphonate + Glyceraldehyde_3_phosphate |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 50 mM Tris-HCl, 1 mM EDTA, 40 µg/ml glycerol phosphate dehydrogenase
PH: 7.5
Temperature: 36 |
- [fructose-bisphosphate aldolase(Enzyme) wildtype]
D_Fructose_1,6_bisphosphate -> D_Glyceraldehyde_3_phosphate + Glycerone_phosphate |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 0.04 mM Tris-Cl
PH: 7.5
Temperature: 25 |
- [fructose-bisphosphate aldolase(Enzyme) wildtype]
D_Fructose_1,6_bisphosphate -> D_Glyceraldehyde_3_phosphate + Glycerone_phosphate |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 0.04 mM Tris-Cl
PH: 7.5
Temperature: 25 |
- [fructose-bisphosphate aldolase(Enzyme) wildtype]
D_Fructose_1,6_bisphosphate -> D_Glyceraldehyde_3_phosphate + Glycerone_phosphate |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 0.04 mM Tris-Cl
PH: 7.5
Temperature: 25 |
uniprot:P00883 [fructose-bisphosphate aldolase(Enzyme) wildtype]
D_Fructose_1,6_bisphosphate -> Glycerone_phosphate + D_Glyceraldehyde_3_phosphate |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 0.04 mM Tris-Cl
PH: 7.5
Temperature: 25 |
uniprot:P79226 [fructose-bisphosphate aldolase(Enzyme) wildtype]
D_Fructose_1,6_bisphosphate -> Glycerone_phosphate + D_Glyceraldehyde_3_phosphate |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 0.04 mM Tris-Cl
PH: 7.5
Temperature: 25 |
uniprot:P79226 [fructose-bisphosphate aldolase(Enzyme) wildtype]
D_Fructose_1,6_bisphosphate -> Glycerone_phosphate + D_Glyceraldehyde_3_phosphate |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 0.04 mM Tris-Cl
PH: 7.5
Temperature: 25 |
- [fructose-bisphosphate aldolase(Enzyme) wildtype]
D_Fructose_1,6_bisphosphate -> Glycerone_phosphate + D_Glyceraldehyde_3_phosphate |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 0.04 mM Tris-Cl
PH: 7.5
Temperature: 25 |
uniprot:P79226 [fructose-bisphosphate aldolase(Enzyme) wildtype]
D_Fructose_1,6_bisphosphate -> D_Glyceraldehyde_3_phosphate + Glycerone_phosphate |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 0.04 mM Tris-Cl
PH: 7.5
Temperature: 25 |
uniprot:P79226 [fructose-bisphosphate aldolase(Enzyme) wildtype]
D_Fructose_1,6_bisphosphate -> Glycerone_phosphate + D_Glyceraldehyde_3_phosphate |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 0.04 mM Tris-Cl
PH: 7.5
Temperature: 25 |
- [fructose-bisphosphate aldolase(Enzyme) wildtype]
D_Fructose_1,6_bisphosphate -> D_Glyceraldehyde_3_phosphate + Glycerone_phosphate |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 0.04 mM Tris-Cl
PH: 7.5
Temperature: 25 |
- [fructose-bisphosphate aldolase(Enzyme) wildtype]
D_Fructose_1_phosphate -> D_Glyceraldehyde + Glycerone_phosphate |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 0.04 mM Tris-Cl
PH: 7.5
Temperature: 25 |
- [fructose-bisphosphate aldolase(Enzyme) wildtype]
D_Fructose_1_phosphate -> D_Glyceraldehyde + Glycerone_phosphate |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 0.04 mM Tris-Cl
PH: 7.5
Temperature: 25 |
- [fructose-bisphosphate aldolase(Enzyme) wildtype]
D_Fructose_1_phosphate -> Glycerone_phosphate + D_Glyceraldehyde |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 0.04 mM Tris-Cl
PH: 7.5
Temperature: 25 |
uniprot:P79226 [fructose-bisphosphate aldolase(Enzyme) wildtype]
D_Fructose_1_phosphate -> D_Glyceraldehyde + Glycerone_phosphate |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 0.04 mM Tris-Cl
PH: 7.5
Temperature: 25 |
uniprot:P00883 [fructose-bisphosphate aldolase(Enzyme) wildtype]
D_Fructose_1_phosphate -> Glycerone_phosphate + D_Glyceraldehyde |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 0.04 mM Tris-Cl
PH: 7.5
Temperature: 25 |
- [fructose-bisphosphate aldolase(Enzyme) wildtype]
D_Fructose_1_phosphate -> D_Glyceraldehyde + Glycerone_phosphate |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 0.04 mM Tris-Cl
PH: 7.5
Temperature: 25 |
- [fructose-bisphosphate aldolase(Enzyme) wildtype]
D_Fructose_1,6_bisphosphate -> D_Glyceraldehyde_3_phosphate + Glycerone_phosphate |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 0.12 mM Tris-HCl, 0.63 µM DPNH, 2 mg BSA
PH: 7.5
Temperature: 25 |
- [fructose-bisphosphate aldolase(Enzyme) wildtype]
D_Fructose_1_phosphate -> Glycerone_phosphate + D_Glyceraldehyde |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 0.12 mM Tris-HCl, 0.63 µM DPNH, 2 mg BSA
PH: 7.5
Temperature: 25 |
- [fructose-bisphosphate aldolase(Enzyme) wildtype]
D_Fructose_1_phosphate -> Glycerone_phosphate + D_Glyceraldehyde |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 0.04 mM Tris-Cl
PH: 7.5
Temperature: 25 |
uniprot:Q42728 [fructose-bisphosphate aldolase(Enzyme) wildtype class II isoenzyme]
D_Fructose_1,6_bisphosphate -> D_Glyceraldehyde_3_phosphate + Glycerone_phosphate |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 50 mM Tris/HCl, 4.5 mM MgCl2, 1 U/ml triosephosphate isomerase, 1 U/ml glycerol-3-phosphate dehydrogenase, 210 µM NADH
PH: 7.5
Temperature: 20 |
uniprot:P53446 [fructose-bisphosphate aldolase(Enzyme) wildtype non-muscle type]
D_Fructose_1,6_bisphosphate -> Glycerone_phosphate + D_Glyceraldehyde_3_phosphate |
(( (( (E * kcat) ) * S) ) / (Km + S)) |
|
buffer: 0.1 M Tris-HCl
PH: 7.5
Temperature: 30 |
uniprot:P53446 [fructose-bisphosphate aldolase(Enzyme) wildtype pLA-II]
D_Fructose_1,6_bisphosphate -> Glycerone_phosphate + D_Glyceraldehyde_3_phosphate |
(( (( (E * kcat) ) * S) ) / (Km + S)) |
|
buffer: 0.1 M Tris-HCl
PH: 7.5
Temperature: 30 |
uniprot:P53445 [fructose-bisphosphate aldolase(Enzyme) wildtype pLA-I]
D_Fructose_1,6_bisphosphate -> D_Glyceraldehyde_3_phosphate + Glycerone_phosphate |
(( (( (E * kcat) ) * S) ) / (Km + S)) |
|
buffer: 0.1 M Tris-HCl
PH: 7.5
Temperature: 30 |
uniprot:P53445 [fructose-bisphosphate aldolase(Enzyme) wildtype muscle-type]
D_Fructose_1,6_bisphosphate -> Glycerone_phosphate + D_Glyceraldehyde_3_phosphate |
(( (( (E * kcat) ) * S) ) / (Km + S)) |
|
buffer: 0.1 M Tris-HCl
PH: 7.5
Temperature: 30 |
uniprot:Q42729 [fructose-bisphosphate aldolase(Enzyme) wildtype class I isoenzyme]
D_Fructose_1,6_bisphosphate -> Glycerone_phosphate + D_Glyceraldehyde_3_phosphate |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 50 mM Tris/HCl, 4.5 mM MgCl2, 1 U/ml triosephosphate isomerase, 1 U/ml glycerol-3-phosphate dehydrogenase, 210 µM NADH
PH: 7.5
Temperature: 20 |
uniprot:P53446 [fructose-bisphosphate aldolase(Enzyme) wildtype pLA-II]
D_Fructose_1_phosphate -> Glycerone_phosphate + D_Glyceraldehyde |
(( (( (E * kcat) ) * S) ) / (Km + S)) |
|
buffer: 0.1 M Tris-HCl
PH: 7.5
Temperature: 30 |
uniprot:P53445 [fructose-bisphosphate aldolase(Enzyme) wildtype muscle-type]
D_Fructose_1_phosphate -> D_Glyceraldehyde + Glycerone_phosphate |
(( (( (E * kcat) ) * S) ) / (Km + S)) |
|
buffer: 0.1 M Tris-HCl
PH: 7.5
Temperature: 30 |
uniprot:P53446 [fructose-bisphosphate aldolase(Enzyme) wildtype non-muscle type]
D_Fructose_1_phosphate -> Glycerone_phosphate + D_Glyceraldehyde |
(( (( (E * kcat) ) * S) ) / (Km + S)) |
|
buffer: 0.1 M Tris-HCl
PH: 7.5
Temperature: 30 |
uniprot:P53445 [fructose-bisphosphate aldolase(Enzyme) wildtype pLA-I]
D_Fructose_1_phosphate -> Glycerone_phosphate + D_Glyceraldehyde |
(( (( (E * kcat) ) * S) ) / (Km + S)) |
|
buffer: 0.1 M Tris-HCl
PH: 7.5
Temperature: 30 |
- [fructose-bisphosphate aldolase(Enzyme) wildtype]
D_Fructose_1,6_bisphosphate -> D_Glyceraldehyde_3_phosphate + Glycerone_phosphate |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 20 mM potassium phosphate, 1 unit triosephosphate isomerase, 1 unit glycerol-3-phosphate dehydrogenase
PH: 7.5
Temperature: 20 |
- [fructose-bisphosphate aldolase(Enzyme) wildtype]
D_Fructose_1,6_bisphosphate -> Glycerone_phosphate + D_Glyceraldehyde_3_phosphate |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 20 mM potassium phosphate, 1 unit triosephosphate isomerase, 1 unit glycerol-3-phosphate dehydrogenase
PH: 7.5
Temperature: 20 |
uniprot:P0AB71 [fructose-bisphosphate aldolase(Enzyme) mutant D109A]
D_Fructose_1,6_bisphosphate -> Glycerone_phosphate + D_Glyceraldehyde_3_phosphate |
(( (( (E * kcat) ) * S) ) / (Km + S)) |
|
buffer: 50 mM Tris-HCl, 5 µM Zn2+, 20 mM NADH, 2 µl of 10 mg/ml mixture of triosephosphate isomerase/glycerol-3-phosphate dehydrogenase
PH: 8
Temperature: 30 |
uniprot:P0AB71 [fructose-bisphosphate aldolase(Enzyme) wildtype class II isoenzymee]
D_Fructose_1,6_bisphosphate -> Glycerone_phosphate + D_Glyceraldehyde_3_phosphate |
(( (( (E * kcat) ) * S) ) / (Km + S)) |
|
buffer: 50 mM Tris-HCl, 5 µM Zn2+, 20 mM NADH, 2 µl of 10 mg/ml mixture of triosephosphate isomerase/glycerol-3-phosphate dehydrogenase
PH: 8
Temperature: 30 |
uniprot:P0AB71 [fructose-bisphosphate aldolase(Enzyme) mutant D288A]
D_Fructose_1,6_bisphosphate -> D_Glyceraldehyde_3_phosphate + Glycerone_phosphate |
(( (( (E * kcat) ) * S) ) / (Km + S)) |
|
buffer: 50 mM Tris-HCl, 5 µM Zn2+, 20 mM NADH, 2 µl of 10 mg/ml mixture of triosephosphate isomerase/glycerol-3-phosphate dehydrogenase
PH: 8
Temperature: 30 |
uniprot:P0AB71 [fructose-bisphosphate aldolase(Enzyme) mutant D144A]
D_Fructose_1,6_bisphosphate -> D_Glyceraldehyde_3_phosphate + Glycerone_phosphate |
(( (( (E * kcat) ) * S) ) / (Km + S)) |
|
buffer: 50 mM Tris-HCl, 5 µM Zn2+, 20 mM NADH, 2 µl of 10 mg/ml mixture of triosephosphate isomerase/glycerol-3-phosphate dehydrogenase
PH: 8
Temperature: 30 |
uniprot:A1YZ23 [fructose-bisphosphate aldolase(Enzyme) wildtype]
D_Fructose_1,6_bisphosphate -> Glycerone_phosphate + D_Glyceraldehyde_3_phosphate |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 50 mM Tris-HCl
PH: 8.5
Temperature: 60 |
uniprot:P0AB71 [fructose-bisphosphate aldolase(Enzyme) mutant D329A]
D_Fructose_1,6_bisphosphate -> Glycerone_phosphate + D_Glyceraldehyde_3_phosphate |
(( (( (E * kcat) ) * S) ) / (Km + S)) |
|
buffer: 50 mM Tris-HCl, 0.1 M potassium acetate, 2 mM potassium hexacyanoferrate (III)
PH: 8
Temperature: 30 |
uniprot:P0AB71 [fructose-bisphosphate aldolase(Enzyme) wildtype class II isoenzyme]
D_Fructose_1,6_bisphosphate -> D_Glyceraldehyde_3_phosphate + Glycerone_phosphate |
(( (( (E * kcat) ) * S) ) / (Km + S)) |
|
buffer: 50 mM Tris-HCl, 0.1 M potassium acetate, 2 mM potassium hexacyanoferrate (III)
PH: 8
Temperature: 30 |
uniprot:P0AB71 [fructose-bisphosphate aldolase(Enzyme) mutant D144A]
D_Fructose_1,6_bisphosphate -> Glycerone_phosphate + D_Glyceraldehyde_3_phosphate |
(( (( (E * kcat) ) * S) ) / (Km + S)) |
|
buffer: 50 mM Tris-HCl, 0.1 M potassium acetate, 2 mM potassium hexacyanoferrate (III)
PH: 8
Temperature: 30 |
uniprot:P0AB71 [fructose-bisphosphate aldolase(Enzyme) mutant D290A]
D_Fructose_1,6_bisphosphate -> D_Glyceraldehyde_3_phosphate + Glycerone_phosphate |
(( (( (E * kcat) ) * S) ) / (Km + S)) |
|
buffer: 50 mM Tris-HCl, 0.1 M potassium acetate, 2 mM potassium hexacyanoferrate (III)
PH: 8
Temperature: 30 |
uniprot:P0AB71 [fructose-bisphosphate aldolase(Enzyme) mutant D288A]
D_Fructose_1,6_bisphosphate -> Glycerone_phosphate + D_Glyceraldehyde_3_phosphate |
(( (( (E * kcat) ) * S) ) / (Km + S)) |
|
buffer: 50 mM Tris-HCl, 0.1 M potassium acetate, 2 mM potassium hexacyanoferrate (III)
PH: 8
Temperature: 30 |
uniprot:P0AB71 [fructose-bisphosphate aldolase(Enzyme) mutant N286A]
D_Fructose_1,6_bisphosphate -> D_Glyceraldehyde_3_phosphate + Glycerone_phosphate |
(( (( (E * kcat) ) * S) ) / (Km + S)) |
|
buffer: 50 mM Tris-HCl, 5 µM Zn2+, 20 mM NADH, 2 µl of 10 mg/ml mixture of triosephosphate isomerase/glycerol-3-phosphate dehydrogenase
PH: 8
Temperature: 30 |
uniprot:P0AB71 [fructose-bisphosphate aldolase(Enzyme) mutant D290A]
D_Fructose_1,6_bisphosphate -> Glycerone_phosphate + D_Glyceraldehyde_3_phosphate |
(( (( (E * kcat) ) * S) ) / (Km + S)) |
|
buffer: 50 mM Tris-HCl, 5 µM Zn2+, 20 mM NADH, 2 µl of 10 mg/ml mixture of triosephosphate isomerase/glycerol-3-phosphate dehydrogenase
PH: 8
Temperature: 30 |
uniprot:P0AB71 [fructose-bisphosphate aldolase(Enzyme) mutant N286D]
D_Fructose_1,6_bisphosphate -> Glycerone_phosphate + D_Glyceraldehyde_3_phosphate |
(( (( (E * kcat) ) * S) ) / (Km + S)) |
|
buffer: 50 mM Tris-HCl, 5 µM Zn2+, 20 mM NADH, 2 µl of 10 mg/ml mixture of triosephosphate isomerase/glycerol-3-phosphate dehydrogenase
PH: 8
Temperature: 30 |
uniprot:P0AB71 [fructose-bisphosphate aldolase(Enzyme) mutant D329A]
D_Fructose_1,6_bisphosphate -> D_Glyceraldehyde_3_phosphate + Glycerone_phosphate |
(( (( (E * kcat) ) * S) ) / (Km + S)) |
|
buffer: 50 mM Tris-HCl, 5 µM Zn2+, 20 mM NADH, 2 µl of 10 mg/ml mixture of triosephosphate isomerase/glycerol-3-phosphate dehydrogenase
PH: 8
Temperature: 30 |
uniprot:P0AB71 [fructose-bisphosphate aldolase(Enzyme) mutant N286A]
D_Fructose_1,6_bisphosphate -> Glycerone_phosphate + D_Glyceraldehyde_3_phosphate |
(( (( (E * kcat) ) * S) ) / (Km + S)) |
|
buffer: 50 mM Tris-HCl, 0.1 M potassium acetate, 2 mM potassium hexacyanoferrate (III)
PH: 8
Temperature: 30 |
uniprot:P0AB71 [fructose-bisphosphate aldolase(Enzyme) wildtype class II isoenzyme]
Glycerone_phosphate + D_Glyceraldehyde_3_phosphate -> D_Fructose_1,6_bisphosphate |
(( (( (E * kcat) ) * S) ) / (Km + S)) |
|
buffer: 50 mM Tris-HCl, 0.1 M potassium acetate, 2 mM potassium hexacyanoferrate (III)
PH: 8
Temperature: 30 |
uniprot:P0AB71 [fructose-bisphosphate aldolase(Enzyme) mutant N286A]
D_Fructose_1,6_bisphosphate -> D_Glyceraldehyde_3_phosphate + Glycerone_phosphate |
(( (( (E * kcat) ) * S) ) / (Km + S)) |
|
buffer: 50 mM Tris-HCl, 0.1 M potassium acetate, 2 mM potassium hexacyanoferrate (III)
PH: 8
Temperature: 30 |
uniprot:P0AB71 [fructose-bisphosphate aldolase(Enzyme) mutant D144A]
D_Glyceraldehyde_3_phosphate + Glycerone_phosphate -> D_Fructose_1,6_bisphosphate |
(( (( (E * kcat) ) * S) ) / (Km + S)) |
|
buffer: 50 mM Tris-HCl, 0.1 M potassium acetate, 2 mM potassium hexacyanoferrate (III)
PH: 8
Temperature: 30 |
uniprot:P0AB71 [fructose-bisphosphate aldolase(Enzyme) mutant D288A]
D_Glyceraldehyde_3_phosphate + Glycerone_phosphate -> D_Fructose_1,6_bisphosphate |
(( (( (E * kcat) ) * S) ) / (Km + S)) |
|
buffer: 50 mM Tris-HCl, 0.1 M potassium acetate, 2 mM potassium hexacyanoferrate (III)
PH: 8
Temperature: 30 |
uniprot:P0AB71 [fructose-bisphosphate aldolase(Enzyme) mutant D329A]
D_Glyceraldehyde_3_phosphate + Glycerone_phosphate -> D_Fructose_1,6_bisphosphate |
(( (( (E * kcat) ) * S) ) / (Km + S)) |
|
buffer: 50 mM Tris-HCl, 0.1 M potassium acetate, 2 mM potassium hexacyanoferrate (III)
PH: 8
Temperature: 30 |
uniprot:P0AB71 [fructose-bisphosphate aldolase(Enzyme) mutant D109A]
D_Glyceraldehyde_3_phosphate + Glycerone_phosphate -> D_Fructose_1,6_bisphosphate |
(( (( (E * kcat) ) * S) ) / (Km + S)) |
|
buffer: 50 mM Tris-HCl, 0.1 M potassium acetate, 2 mM potassium hexacyanoferrate (III)
PH: 8
Temperature: 30 |
uniprot:P0AB71 [fructose-bisphosphate aldolase(Enzyme) mutant D290A]
D_Glyceraldehyde_3_phosphate + Glycerone_phosphate -> D_Fructose_1,6_bisphosphate |
(( (( (E * kcat) ) * S) ) / (Km + S)) |
|
buffer: 50 mM Tris-HCl, 0.1 M potassium acetate, 2 mM potassium hexacyanoferrate (III)
PH: 8
Temperature: 30 |
uniprot:P00883 [fructose-bisphosphate aldolase(Enzyme) wildtype]
D_Fructose_1,6_bisphosphate -> D_Glyceraldehyde_3_phosphate + Glycerone_phosphate |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 40 mM Tris-HCl, 0.21 mM NADH, glyceraldehyde dehydrogenase, triosephosphate isomerase 0.66 mg/ml BSA
PH: 7.5
Temperature: 25 |
uniprot:P79226 [fructose-bisphosphate aldolase(Enzyme) wildtype]
D_Fructose_1,6_bisphosphate -> D_Glyceraldehyde_3_phosphate + Glycerone_phosphate |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 40 mM Tris-HCl, 0.21 mM NADH, glyceraldehyde dehydrogenase, triosephosphate isomerase 0.66 mg/ml BSA
PH: 7.5
Temperature: 25 |
uniprot:P00883 [fructose-bisphosphate aldolase(Enzyme) wildtype]
D_Glyceraldehyde_3_phosphate + Glycerone_phosphate -> D_Fructose_1,6_bisphosphate |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 80 mM Tris-HCl
PH: 7.5
Temperature: 25 |
uniprot:P79226 [fructose-bisphosphate aldolase(Enzyme) wildtype]
D_Glyceraldehyde_3_phosphate + Glycerone_phosphate -> D_Fructose_1,6_bisphosphate |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 80 mM Tris-HCl
PH: 7.5
Temperature: 25 |
uniprot:P00883 [fructose-bisphosphate aldolase(Enzyme) wildtype]
D_Glyceraldehyde_3_phosphate + Glycerone_phosphate -> D_Fructose_1,6_bisphosphate |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 80 mM Tris-HCl
PH: 7.5
Temperature: 25 |
uniprot:P79226 [fructose-bisphosphate aldolase(Enzyme) wildtype]
D_Fructose_1_phosphate -> Glycerone_phosphate + D_Glyceraldehyde |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 40 mM Tris-HCl, 0.21 mM NADH, glyceraldehyde dehydrogenase, triosephosphate isomerase 0.66 mg/ml BSA
PH: 7.5
Temperature: 25 |
uniprot:P00883 [fructose-bisphosphate aldolase(Enzyme) wildtype]
D_Fructose_1_phosphate -> D_Glyceraldehyde + Glycerone_phosphate |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 40 mM Tris-HCl, 0.21 mM NADH, glyceraldehyde dehydrogenase, triosephosphate isomerase 0.66 mg/ml BSA
PH: 7.5
Temperature: 25 |
uniprot:P79226 [fructose-bisphosphate aldolase(Enzyme) wildtype]
D_Glyceraldehyde_3_phosphate + Glycerone_phosphate -> D_Fructose_1,6_bisphosphate |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 80 mM Tris-HCl
PH: 7.5
Temperature: 25 |
- [fructose-bisphosphate aldolase(Enzyme) wildtype ALDO C]
D_Fructose_1,6_bisphosphate -> Glycerone_phosphate + D_Glyceraldehyde_3_phosphate |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 40 mM Tris-HCl, 0.21 mM NADH, glyceraldehyde dehydrogenase, triosephosphate isomerase 0.66 mg/ml BSA
PH: 7.5
Temperature: 25 |
- [fructose-bisphosphate aldolase(Enzyme) wildtype ALDO C]
D_Glyceraldehyde_3_phosphate + Glycerone_phosphate -> D_Fructose_1,6_bisphosphate |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 80 mM Tris-HCl
PH: 7.5
Temperature: 25 |
- [fructose-bisphosphate aldolase(Enzyme) wildtype ALDO C]
Glycerone_phosphate + D_Glyceraldehyde_3_phosphate -> D_Fructose_1,6_bisphosphate |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 80 mM Tris-HCl
PH: 7.5
Temperature: 25 |
- [fructose-bisphosphate aldolase(Enzyme) wildtype ALDO C]
D_Fructose_1_phosphate -> Glycerone_phosphate + D_Glyceraldehyde |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 40 mM Tris-HCl, 0.21 mM NADH, glyceraldehyde dehydrogenase, triosephosphate isomerase 0.66 mg/ml BSA
PH: 7.5
Temperature: 25 |
uniprot:P0AB71 [fructose-bisphosphate aldolase(Enzyme) wildtype]
D_Fructose_1,6_bisphosphate -> D_Glyceraldehyde_3_phosphate + Glycerone_phosphate |
(( (( (kcat * E) ) * S) ) / (Km + S)) |
|
buffer: 50 mM Tris-HCl, 20 mM NADH, Glycerol phosphate dehydrogenase, Triosephosphate isomerase
PH: 8
Temperature: 30 |
uniprot:P0AB71 [fructose-bisphosphate aldolase(Enzyme) mutant K325A]
D_Fructose_1,6_bisphosphate -> Glycerone_phosphate + D_Glyceraldehyde_3_phosphate |
(( (( (kcat * E) ) * S) ) / (Km + S)) |
|
buffer: 50 mM Tris-HCl, 20 mM NADH, Glycerol phosphate dehydrogenase, Triosephosphate isomerase
PH: 8
Temperature: 30 |
uniprot:P0AB71 [fructose-bisphosphate aldolase(Enzyme) mutant S61T]
D_Fructose_1,6_bisphosphate -> D_Glyceraldehyde_3_phosphate + Glycerone_phosphate |
(( (( (kcat * E) ) * S) ) / (Km + S)) |
|
buffer: 50 mM Tris-HCl, 20 mM NADH, Glycerol phosphate dehydrogenase, Triosephosphate isomerase
PH: 8
Temperature: 30 |
uniprot:P0AB71 [fructose-bisphosphate aldolase(Enzyme) mutant Q59A]
D_Fructose_1,6_bisphosphate -> Glycerone_phosphate + D_Glyceraldehyde_3_phosphate |
(( (( (kcat * E) ) * S) ) / (Km + S)) |
|
buffer: 50 mM Tris-HCl, 20 mM NADH, Glycerol phosphate dehydrogenase, Triosephosphate isomerase
PH: 8
Temperature: 30 |
uniprot:P0AB71 [fructose-bisphosphate aldolase(Enzyme) mutant N35A]
D_Fructose_1,6_bisphosphate -> D_Glyceraldehyde_3_phosphate + Glycerone_phosphate |
(( (( (kcat * E) ) * S) ) / (Km + S)) |
|
buffer: 50 mM Tris-HCl, 20 mM NADH, Glycerol phosphate dehydrogenase, Triosephosphate isomerase
PH: 8
Temperature: 30 |
uniprot:P0AB71 [fructose-bisphosphate aldolase(Enzyme) mutant S61A]
D_Fructose_1,6_bisphosphate -> Glycerone_phosphate + D_Glyceraldehyde_3_phosphate |
(( (( (kcat * E) ) * S) ) / (Km + S)) |
|
buffer: 50 mM Tris-HCl, 20 mM NADH, Glycerol phosphate dehydrogenase, Triosephosphate isomerase
PH: 8
Temperature: 30 |
uniprot:P0AB71 [fructose-bisphosphate aldolase(Enzyme) mutant S61T/G64T/F67H]
D_Fructose_1,6_bisphosphate -> Glycerone_phosphate + D_Glyceraldehyde_3_phosphate |
(( (( (kcat * E) ) * S) ) / (Km + S)) |
|
buffer: 50 mM Tris-HCl, 20 mM NADH, Glycerol phosphate dehydrogenase, Triosephosphate isomerase
PH: 8
Temperature: 30 |
uniprot:P0AB71 [fructose-bisphosphate aldolase(Enzyme) mutant D288A]
D_Fructose_1,6_bisphosphate -> Glycerone_phosphate + D_Glyceraldehyde_3_phosphate |
(( (( (kcat * E) ) * S) ) / (Km + S)) |
|
buffer: 50 mM Tris-HCl, 20 mM NADH, Glycerol phosphate dehydrogenase, Triosephosphate isomerase
PH: 8
Temperature: 30 |
uniprot:P0AB71 [fructose-bisphosphate aldolase(Enzyme) mutant Q59A/S61T/D288A]
D_Fructose_1,6_bisphosphate -> Glycerone_phosphate + D_Glyceraldehyde_3_phosphate |
(( (( (kcat * E) ) * S) ) / (Km + S)) |
|
buffer: 50 mM Tris-HCl, 20 mM NADH, Glycerol phosphate dehydrogenase, Triosephosphate isomerase
PH: 8
Temperature: 30 |
uniprot:P0AB71 [fructose-bisphosphate aldolase(Enzyme) mutant Q59A/G64T/F67H]
D_Fructose_1,6_bisphosphate -> Glycerone_phosphate + D_Glyceraldehyde_3_phosphate |
(( (( (kcat * E) ) * S) ) / (Km + S)) |
|
buffer: 50 mM Tris-HCl, 20 mM NADH, Glycerol phosphate dehydrogenase, Triosephosphate isomerase
PH: 8
Temperature: 30 |
uniprot:P0AB71 [fructose-bisphosphate aldolase(Enzyme) mutant Q59A/F67H]
D_Fructose_1,6_bisphosphate -> Glycerone_phosphate + D_Glyceraldehyde_3_phosphate |
(( (( (kcat * E) ) * S) ) / (Km + S)) |
|
buffer: 50 mM Tris-HCl, 20 mM NADH, Glycerol phosphate dehydrogenase, Triosephosphate isomerase
PH: 8
Temperature: 30 |
uniprot:P0AB71 [fructose-bisphosphate aldolase(Enzyme) mutant F67H]
D_Fructose_1,6_bisphosphate -> D_Glyceraldehyde_3_phosphate + Glycerone_phosphate |
(( (( (kcat * E) ) * S) ) / (Km + S)) |
|
buffer: 50 mM Tris-HCl, 20 mM NADH, Glycerol phosphate dehydrogenase, Triosephosphate isomerase
PH: 8
Temperature: 30 |
uniprot:P0AB71 [fructose-bisphosphate aldolase(Enzyme) mutant G64T]
D_Fructose_1,6_bisphosphate -> Glycerone_phosphate + D_Glyceraldehyde_3_phosphate |
(( (( (kcat * E) ) * S) ) / (Km + S)) |
|
buffer: 50 mM Tris-HCl, 20 mM NADH, Glycerol phosphate dehydrogenase, Triosephosphate isomerase
PH: 8
Temperature: 30 |
uniprot:P0AB74 [fructose-bisphosphate aldolase(Enzyme) wildtype AgaY]
D_Fructose_1,6_bisphosphate -> Glycerone_phosphate + D_Glyceraldehyde_3_phosphate |
(( (( (kcat * E) ) * S) ) / (Km + S)) |
|
buffer: 50 mM Tris-HCl, 20 mM NADH, Glycerol phosphate dehydrogenase, Triosephosphate isomerase
PH: 8
Temperature: 30 |
uniprot:P0AB71 [fructose-bisphosphate aldolase(Enzyme) mutant N35A]
Hexacyanoferrate(III) + Glycerone_phosphate -> Hydroxypyruvaldehyde_phosphate + Hexacyanoferrate(II) |
(( (( (kcat * E) ) * S) ) / (Km + S)) |
|
buffer: 50 mM Tris-HCl, 0.1 M Potassium acetate
PH: 8
Temperature: 30 |
uniprot:P0AB71 [fructose-bisphosphate aldolase(Enzyme) wildtype]
Glycerone_phosphate + Hexacyanoferrate(III) -> Hydroxypyruvaldehyde_phosphate + Hexacyanoferrate(II) |
(( (( (kcat * E) ) * S) ) / (Km + S)) |
|
buffer: 50 mM Tris-HCl, 0.1 M Potassium acetate
PH: 8
Temperature: 30 |
uniprot:P0AB71 [fructose-bisphosphate aldolase(Enzyme) mutant S61A]
Glycerone_phosphate + Hexacyanoferrate(III) -> Hexacyanoferrate(II) + Hydroxypyruvaldehyde_phosphate |
(( (( (kcat * E) ) * S) ) / (Km + S)) |
|
buffer: 50 mM Tris-HCl, 0.1 M Potassium acetate
PH: 8
Temperature: 30 |
uniprot:P0AB71 [fructose-bisphosphate aldolase(Enzyme) mutant Q59A]
Hexacyanoferrate(III) + Glycerone_phosphate -> Hydroxypyruvaldehyde_phosphate + Hexacyanoferrate(II) |
(( (( (kcat * E) ) * S) ) / (Km + S)) |
|
buffer: 50 mM Tris-HCl, 0.1 M Potassium acetate
PH: 8
Temperature: 30 |
uniprot:P0AB71 [fructose-bisphosphate aldolase(Enzyme) mutant K325A]
Glycerone_phosphate + Hexacyanoferrate(III) -> Hexacyanoferrate(II) + Hydroxypyruvaldehyde_phosphate |
(( (( (kcat * E) ) * S) ) / (Km + S)) |
|
buffer: 50 mM Tris-HCl, 0.1 M Potassium acetate
PH: 8
Temperature: 30 |
uniprot:P0AB71 [fructose-bisphosphate aldolase(Enzyme) mutant S61T]
Glycerone_phosphate + Hexacyanoferrate(III) -> Hexacyanoferrate(II) + Hydroxypyruvaldehyde_phosphate |
(( (( (kcat * E) ) * S) ) / (Km + S)) |
|
buffer: 50 mM Tris-HCl, 0.1 M Potassium acetate
PH: 8
Temperature: 30 |
uniprot:P0AB71 [fructose-bisphosphate aldolase(Enzyme) mutant Q59A]
Hexacyanoferrate(III) + D_Fructose_1,6_bisphosphate -> Hexacyanoferrate(II) + D_Glyceraldehyde_3_phosphate + Hydroxypyruvaldehyde_phosphate |
(( (( (kcat * E) ) * S) ) / (Km + S)) |
|
buffer: 50 mM Tris-HCl, 0.1 M Potassium acetate
PH: 8
Temperature: 30 |
uniprot:P0AB71 [fructose-bisphosphate aldolase(Enzyme) mutant N35A]
D_Fructose_1,6_bisphosphate + Hexacyanoferrate(III) -> Hydroxypyruvaldehyde_phosphate + Hexacyanoferrate(II) + D_Glyceraldehyde_3_phosphate |
(( (( (kcat * E) ) * S) ) / (Km + S)) |
|
buffer: 50 mM Tris-HCl, 0.1 M Potassium acetate
PH: 8
Temperature: 30 |
uniprot:P0AB71 [fructose-bisphosphate aldolase(Enzyme) mutant K325A]
Hexacyanoferrate(III) + D_Fructose_1,6_bisphosphate -> Hexacyanoferrate(II) + D_Glyceraldehyde_3_phosphate + Hydroxypyruvaldehyde_phosphate |
(( (( (kcat * E) ) * S) ) / (Km + S)) |
|
buffer: 50 mM Tris-HCl, 0.1 M Potassium acetate
PH: 8
Temperature: 30 |
uniprot:P0AB71 [fructose-bisphosphate aldolase(Enzyme) wildtype]
D_Fructose_1,6_bisphosphate + Hexacyanoferrate(III) -> Hexacyanoferrate(II) + Hydroxypyruvaldehyde_phosphate + D_Glyceraldehyde_3_phosphate |
(( (( (kcat * E) ) * S) ) / (Km + S)) |
|
buffer: 50 mM Tris-HCl, 0.1 M Potassium acetate
PH: 8
Temperature: 30 |
uniprot:P0AB71 [fructose-bisphosphate aldolase(Enzyme) mutant S61A]
Hexacyanoferrate(III) + D_Fructose_1,6_bisphosphate -> D_Glyceraldehyde_3_phosphate + Hydroxypyruvaldehyde_phosphate + Hexacyanoferrate(II) |
(( (( (kcat * E) ) * S) ) / (Km + S)) |
|
buffer: 50 mM Tris-HCl, 0.1 M Potassium acetate
PH: 8
Temperature: 30 |
uniprot:P0AB71 [fructose-bisphosphate aldolase(Enzyme) mutant S61T]
D_Fructose_1,6_bisphosphate + Hexacyanoferrate(III) -> D_Glyceraldehyde_3_phosphate + Hexacyanoferrate(II) + Hydroxypyruvaldehyde_phosphate |
(( (( (kcat * E) ) * S) ) / (Km + S)) |
|
buffer: 50 mM Tris-HCl, 0.1 M Potassium acetate
PH: 8
Temperature: 30 |
- [fructose-bisphosphate aldolase(Enzyme) wildtype]
D_Fructose_1,6_bisphosphate -> Glycerone_phosphate + D_Glyceraldehyde_3_phosphate |
(( (( (( (Vf * Vr) ) * B) ) / (( (( (( (( (( (KmB * Vr) ) + (B * Vr)) ) + (( (( (KmC * A) ) * Vf) ) / Keq)) ) + (( (( (KmA * C) ) * Vf) ) / Keq)) ) + (( (( (A * C) ) * Vf) ) / Keq)) ) + (( (( (B * A) ) * Vr) ) / Ki))) ) - (( (( (( (( (Vf * Vr) ) * A) ) * C) ) / Keq) ) / (( (( (( (( (( (KmB * Vr) ) + (B * Vr)) ) + (( (( (KmC * A) ) * Vf) ) / Keq)) ) + (( (( (KmA * C) ) * Vf) ) / Keq)) ) + (( (( (A * C) ) * Vf) ) / Keq)) ) + (( (( (B * A) ) * Vr) ) / Ki)))) |
|
buffer: 80 mM Tris-HCl, 100 mM KCl, 0.3 mM NADH, Glycerolphosphate dehydrogenase, Triosephosphate isomerase
PH: 7.6
Temperature: 30 |
uniprot:O97447 [D-Tagatose 1,6-bisphosphate]
D_Fructose_1,6_bisphosphate -> Glycerone_phosphate + D_Glyceraldehyde_3_phosphate |
(( (( (kcat * E) ) * S) ) / (( (Km * (1 + (I / Ki))) ) + S)) |
|
buffer: 50 mM K+Hepes, 200 µM NADH, 5 U/ml Triose-phosphate isomerase, 2 U/ml Glycerol-3-phosphate dehydrogenase
PH: 7.5
Temperature: 25 |
uniprot:O97447 [Phosphoglycolohydroxamate]
D_Fructose_1,6_bisphosphate -> D_Glyceraldehyde_3_phosphate + Glycerone_phosphate |
(( (( (kcat * E) ) * S) ) / (( (Km * (1 + (I / Ki))) ) + S)) |
|
buffer: 50 mM K+Hepes, 200 µM NADH, 5 U/ml Triose-phosphate isomerase, 2 U/ml Glycerol-3-phosphate dehydrogenase
PH: 7.5
Temperature: 25 |
uniprot:G4NDL4 [fructose-bisphosphate aldolase(Enzyme) wildtype class II]
D_Fructose_1,6_bisphosphate -> Glycerone_phosphate + D_Glyceraldehyde_3_phosphate |
(( (( (kcat * E) ) * S) ) / (Km + S)) |
|
buffer: 100 mM potassium acetate, 50 mM Tris-HCl, 0.2 mg/ml BSA, 0.3 mM NADH, 0.2 U/ml rabbit muscle alpha-glycerophosphate dehydrogenase, 2.25 U/ml rabbit muscle triose phosphate isomerase
PH: 8
Temperature: 30 |
uniprot:O97447 [fructose-bisphosphate aldolase(Enzyme) wildtype class II]
D_Fructose_1,6_bisphosphate -> Glycerone_phosphate + D_Glyceraldehyde_3_phosphate |
(( (( (kcat * E) ) * S) ) / (Km + S)) |
|
buffer: 50 mM K+Hepes, 200 µM NADH, 5 U/ml Triose-phosphate isomerase, 2 U/ml Glycerol-3-phosphate dehydrogenase
PH: 7.5
Temperature: 25 |
uniprot:O52402 [fructose-bisphosphate aldolase(Enzyme) mutant 2-15B2]
D_Fructose_1,6_bisphosphate -> D_Glyceraldehyde_3_phosphate + Glycerone_phosphate |
(( (( (kcat * E) ) * S) ) / (Km + S)) |
|
buffer: 50 mM Tris-HCl, 0.1 M potassium acetate, 0.6 mM NADH, 100 mg/ml glycerol 3-phosphate dehydrogenase
PH: 8
Temperature: 30 |
uniprot:O52402 [fructose-bisphosphate aldolase(Enzyme) wildtype]
D_Fructose_1,6_bisphosphate -> D_Glyceraldehyde_3_phosphate + Glycerone_phosphate |
(( (( (kcat * E) ) * S) ) / (Km + S)) |
|
buffer: 50 mM Tris-HCl, 0.1 M potassium acetate, 0.6 mM NADH, 100 mg/ml glycerol 3-phosphate dehydrogenase
PH: 8
Temperature: 30 |
uniprot:O52402 [fructose-bisphosphate aldolase(Enzyme) mutant 1-44F2]
D_Fructose_1,6_bisphosphate -> D_Glyceraldehyde_3_phosphate + Glycerone_phosphate |
(( (( (kcat * E) ) * S) ) / (Km + S)) |
|
buffer: 50 mM Tris-HCl, 0.1 M potassium acetate, 0.6 mM NADH, 100 mg/ml glycerol 3-phosphate dehydrogenase
PH: 8
Temperature: 30 |
uniprot:O52402 [fructose-bisphosphate aldolase(Enzyme) mutant 4-4C10]
D_Fructose_1,6_bisphosphate -> D_Glyceraldehyde_3_phosphate + Glycerone_phosphate |
(( (( (kcat * E) ) * S) ) / (Km + S)) |
|
buffer: 50 mM Tris-HCl, 0.1 M potassium acetate, 0.6 mM NADH, 100 mg/ml glycerol 3-phosphate dehydrogenase
PH: 8
Temperature: 30 |
uniprot:O52402 [fructose-bisphosphate aldolase(Enzyme) mutant 3-4C10]
D_Fructose_1,6_bisphosphate -> D_Glyceraldehyde_3_phosphate + Glycerone_phosphate |
(( (( (kcat * E) ) * S) ) / (Km + S)) |
|
buffer: 50 mM Tris-HCl, 0.1 M potassium acetate, 0.6 mM NADH, 100 mg/ml glycerol 3-phosphate dehydrogenase
PH: 8
Temperature: 30 |
uniprot:P0AB71 [fructose-bisphosphate aldolase(Enzyme) wildtype]
D_Fructose_1,6_bisphosphate -> D_Glyceraldehyde_3_phosphate + Glycerone_phosphate |
(( (( (kcat * E) ) * S) ) / (Km + S)) |
|
buffer: 50 mM Tris-HCl, 0.1 M potassium acetate, 0.6 mM NADH, 100 mg/ml glycerol 3-phosphate dehydrogenase
PH: 8
Temperature: 30 |
uniprot:O52402 [fructose-bisphosphate aldolase(Enzyme) mutant I71/A210]
D_Fructose_1,6_bisphosphate -> Glycerone_phosphate + D_Glyceraldehyde_3_phosphate |
(( (( (kcat * E) ) * S) ) / (Km + S)) |
|
buffer: 50 mM Tris-HCl, 0.1 M potassium acetate, 0.6 mM NADH, 100 mg/ml glycerol 3-phosphate dehydrogenase
PH: 8
Temperature: 30 |
uniprot:O52402 [fructose-bisphosphate aldolase(Enzyme) mutant K71/V210]
D_Fructose_1,6_bisphosphate -> Glycerone_phosphate + D_Glyceraldehyde_3_phosphate |
(( (( (kcat * E) ) * S) ) / (Km + S)) |
|
buffer: 50 mM Tris-HCl, 0.1 M potassium acetate, 0.6 mM NADH, 100 mg/ml glycerol 3-phosphate dehydrogenase
PH: 8
Temperature: 30 |
uniprot:O52402 [fructose-bisphosphate aldolase(Enzyme) mutant 4-43D6]
D_Fructose_1,6_bisphosphate -> Glycerone_phosphate + D_Glyceraldehyde_3_phosphate |
(( (( (kcat * E) ) * S) ) / (Km + S)) |
|
buffer: 50 mM Tris-HCl, 0.1 M potassium acetate, 0.6 mM NADH, 100 mg/ml glycerol 3-phosphate dehydrogenase
PH: 8
Temperature: 30 |
uniprot:P67475 [fructose-bisphosphate aldolase(Enzyme) wildtype]
D_Fructose_1,6_bisphosphate -> D_Glyceraldehyde_3_phosphate + Glycerone_phosphate |
(( (( (kcat * E) ) * S) ) / (Km + S)) |
|
buffer: 25 mM Hepes
PH: 7.3
Temperature: 28 |
- [fructose-bisphosphate aldolase(Enzyme) wildtype class I]
D_Fructose_1,6_bisphosphate -> Glycerone_phosphate + D_Glyceraldehyde_3_phosphate |
(( (Vmax * (S ^ h)) ) / (( (S_half ^ h) ) + (S ^ h))) |
|
buffer: 50 mM Tris-HCl, 0.01 mM NADH, 1 mM EDTA, 0.2 mM EGTA, 10 unit/ml triosephosphate isomerase, 1 unit/ml glycerol-3-phosphate dehydrogenase
PH: 7.2
Temperature: 30 |
- [fructose-bisphosphate aldolase(Enzyme) wildtype class I]
Glycerone_phosphate + D_Glyceraldehyde_3_phosphate -> D_Fructose_1,6_bisphosphate |
(( (Vmax * (S ^ h)) ) / (( (S_half ^ h) ) + (S ^ h))) |
|
buffer: 50 mM Tris-HCl, 0.15 mM NAD+, 5 mM MgCl2, 1 mM EDTA, 0.2 mM EGTA, 10 unit/ml triosephosphate isomerase, 1 unit/ml FBPase, 1 U/ml hexose-P-isomerase, 1 U/ml Glucose-6-phosphate dehydrogenase
PH: 7.2
Temperature: 30 |
- [fructose-bisphosphate aldolase(Enzyme) wildtype class I]
D_Fructose_1_phosphate -> Glycerone_phosphate + D_Glyceraldehyde |
(( (Vmax * (S ^ h)) ) / (( (S_half ^ h) ) + (S ^ h))) |
|
buffer: 50 mM Tris-HCl, 0.01 mM NADH, 1 mM EDTA, 0.2 mM EGTA, 10 unit/ml triosephosphate isomerase, 1 unit/ml glycerol-3-phosphate dehydrogenase
PH: 7.2
Temperature: 30 |
- [fructose-bisphosphate aldolase(Enzyme) wildtype class I]
Sedoheptulose_1,7_bisphosphate -> Glycerone_phosphate + D_Erythrose_4_phosphate |
(( (Vmax * (S ^ h)) ) / (( (S_half ^ h) ) + (S ^ h))) |
|
buffer: 50 mM Tris-HCl, 0.01 mM NADH, 1 mM EDTA, 0.2 mM EGTA, 10 unit/ml triosephosphate isomerase, 1 unit/ml glycerol-3-phosphate dehydrogenase
PH: 7.2
Temperature: 30 |
uniprot:P08440 [fructose-bisphosphate aldolase(Enzyme) wildtype]
D_Fructose_1,6_bisphosphate -> D_Glyceraldehyde_3_phosphate + Glycerone_phosphate |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 0.1 M triethanolamine-HCl, 10 mM EDTA, 1 mM DTT, NADH, alpha-glycerophosphate dehydrogenase/triosephosphatee isomerase
PH: 7.3
Temperature: 22 |
uniprot:P08440 [fructose-bisphosphate aldolase(Enzyme) wildtype]
D_Fructose_1_phosphate -> Glycerone_phosphate + D_Glyceraldehyde |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 10 mM EDTA, 1 mM DTT, NADH, alpha-glycerophosphate dehydrogenase/triosephosphate isomerase
PH: 7.3
Temperature: 22 |
uniprot:P00883 [fructose-bisphosphate aldolase(Enzyme) mutant E189A]
D_Fructose_1,6_bisphosphate -> D_Glyceraldehyde_3_phosphate + Glycerone_phosphate |
(( (( (kcat * E) ) * S) ) / (Km + S)) |
|
buffer: 100 mM Triethanolamine acetate, 1 mM EDTA, 0.42 mM NADH, 1 µg/ml triose-phosphate isomerase, 10 µg/ml glycerol-3-phosphate dehydrogenase
PH: 7.6
Temperature: 22 |
uniprot:P00883 [fructose-bisphosphate aldolase(Enzyme) mutant E187A]
D_Fructose_1,6_bisphosphate -> D_Glyceraldehyde_3_phosphate + Glycerone_phosphate |
(( (( (kcat * E) ) * S) ) / (Km + S)) |
|
buffer: 100 mM Triethanolamine acetate, 1 mM EDTA, 0.42 mM NADH, 1 µg/ml triose-phosphate isomerase, 10 µg/ml glycerol-3-phosphate dehydrogenase
PH: 7.6
Temperature: 22 |
uniprot:P00883 [fructose-bisphosphate aldolase(Enzyme) wildtype]
D_Fructose_1,6_bisphosphate -> Glycerone_phosphate + D_Glyceraldehyde_3_phosphate |
(( (( (kcat * E) ) * S) ) / (Km + S)) |
|
buffer: 100 mM Triethanolamine acetate, 1 mM EDTA, 0.42 mM NADH, 1 µg/ml triose-phosphate isomerase, 10 µg/ml glycerol-3-phosphate dehydrogenase
PH: 7.6
Temperature: 22 |
uniprot:P00883 [fructose-bisphosphate aldolase(Enzyme) mutant E187Q]
D_Fructose_1,6_bisphosphate -> D_Glyceraldehyde_3_phosphate + Glycerone_phosphate |
(( (( (kcat * E) ) * S) ) / (Km + S)) |
|
buffer: 100 mM Triethanolamine acetate, 1 mM EDTA, 0.42 mM NADH, 1 µg/ml triose-phosphate isomerase, 10 µg/ml glycerol-3-phosphate dehydrogenase
PH: 7.6
Temperature: 22 |
uniprot:P00883 [fructose-bisphosphate aldolase(Enzyme) mutant E189Q]
D_Fructose_1,6_bisphosphate -> Glycerone_phosphate + D_Glyceraldehyde_3_phosphate |
(( (( (kcat * E) ) * S) ) / (Km + S)) |
|
buffer: 100 mM Triethanolamine acetate, 1 mM EDTA, 0.42 mM NADH, 1 µg/ml triose-phosphate isomerase, 10 µg/ml glycerol-3-phosphate dehydrogenase
PH: 7.6
Temperature: 22 |
uniprot:Q9SJQ9 [Oxidized glutathione]
D_Fructose_1,6_bisphosphate -> D_Glyceraldehyde_3_phosphate + Glycerone_phosphate |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 100 mM Tris-HCl, 0.25 mM NADH, 100 mM KCl, 25 mM EDTA, 10 µg/ml glycerol 3-phosphate dehydrogeanase/triosephosphate isomerase
PH: 7.8
Temperature: 36 |
uniprot:Q9SJQ9 [fructose-bisphosphate aldolase(Enzyme) wildtype N-terminal His-tagged]
D_Fructose_1,6_bisphosphate -> D_Glyceraldehyde_3_phosphate + Glycerone_phosphate |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 100 mM Tris-HCl, 0.25 mM NADH, 100 mM KCl, 25 mM EDTA, 10 µg/ml glycerol 3-phosphate dehydrogeanase/triosephosphate isomerase
PH: 7.8
Temperature: 36 |
uniprot:P00884 [fructose-bisphosphate aldolase(Enzyme) wildtype]
D_Fructose_1_phosphate -> Glycerone_phosphate + D_Glyceraldehyde |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 25 mM Tris-HCl, 1 mM MgCl2, 0.15 mM NADH, rabbit muscle triose phosphate isomerase (3.5 U/ml), glycerol‐3‐phosphate dehydrogenase (1 U/ml)
PH: 7.5
Temperature: 36 |
uniprot:P00884 [fructose-bisphosphate aldolase(Enzyme) wildtype]
Sedoheptulose_1_phosphate -> Glycerone_phosphate + Erythrose |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 25 mM Tris-HCl, 1 mM MgCl2, 0.15 mM NADH, rabbit muscle triose phosphate isomerase (3.5 U/ml), glycerol‐3‐phosphate dehydrogenase (1 U/ml)
PH: 7.5
Temperature: 36 |
uniprot:K9YER0 [fructose-bisphosphate aldolase(Enzyme) wildtype]
D_Fructose_1,6_bisphosphate -> Glycerone_phosphate + D_Glyceraldehyde_3_phosphate |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 50 mM Tris-HCl, 1 U glycerol phosphate dehydrogenase, 1 U triose phosphate isomerase, 0.2 mM NADH
PH: 8
Temperature: 30 |