EC: 5.3.99.3

prostaglandin-E synthase ((5Z,13E)-(15S)-9alpha,11alpha-Epidioxy-15-hydroxyprosta-5,13-dienoate E-isomerase)

enzyme lambda metabolic reaction experiment
uniprot:Q9N0A4 [prostaglandin-E synthase(Enzyme) wildtype His-tagged]

Prostaglandin_H2 -> Prostaglandin_E2		 (( (Vmax * S) ) / (Km + S)) buffer: 0.1 M Potassium phosphate, 0.5 mM DTT
PH: 6.5
Temperature: 24
uniprot:O14684 [prostaglandin-E synthase(Enzyme) wildtype]

Prostaglandin_H2 -> Prostaglandin_E2		 (( (Vmax * S) ) / (Km + S)) buffer: 100 mM KHPO4, 2 mM EDTA, 2.5 mM reduced GSH
PH: 7
Temperature: 20
uniprot:O14684 [Reduced glutathione]

Prostaglandin_H2 -> Prostaglandin_D2		 (( (Vmax * S) ) / (Km + S)) buffer: 20 mM Na2HPO4/NaH2PO4
PH: 7.2
Temperature: 22
uniprot:O14684 [Reduced glutathione]

Prostaglandin_H2 -> Prostaglandin_D2		 (( (Vmax * S) ) / (Km + S)) buffer: 20 mM Na2HPO4/NaH2PO4
PH: 7.2
Temperature: 22
uniprot:O14684 [Reduced glutathione]

Prostaglandin_H2 -> Prostaglandin_D2		 (( (Vmax * S) ) / (Km + S)) buffer: 20 mM Na2HPO4/NaH2PO4
PH: 7.2
Temperature: 22
uniprot:O14684 [Reduced glutathione]

Prostaglandin_H2 -> Prostaglandin_D2		 (( (Vmax * S) ) / (Km + S)) buffer: 20 mM Na2HPO4/NaH2PO4
PH: 7.2
Temperature: 22
uniprot:O14684 [Reduced glutathione]

Prostaglandin_H2 -> Prostaglandin_D2		 (( (Vmax * S) ) / (Km + S)) buffer: 20 mM Na2HPO4/NaH2PO4
PH: 7.2
Temperature: 22
uniprot:O14684 [Reduced glutathione]

Prostaglandin_H2 -> Prostaglandin_D2		 (( (Vmax * S) ) / (Km + S)) buffer: 20 mM Na2HPO4/NaH2PO4
PH: 7.2
Temperature: 22
uniprot:O14684 [Reduced glutathione]

Prostaglandin_H2 -> Prostaglandin_E2		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 0.1 M potassium phosphate, 0.1 % Triton X-100
PH: 7.4
Temperature: 37
uniprot:O14684 [Reduced glutathione]

Prostaglandin_H2 -> Prostaglandin_E2		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 0.1 M potassium phosphate, 0.1% Triton X-100
PH: 7.4
Temperature: 37
uniprot:O14684 [Reduced glutathione]

Prostaglandin_G2 -> 15_Hydroperoxy_prostaglandin_E2		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 0.1 M potassium phosphate
PH: 7.4
Temperature: 37
uniprot:O14684 [Reduced glutathione]

Prostaglandin_H2 -> Prostaglandin_D2		 (( (Vmax * S) ) / (Km + S)) buffer: 20 mM Na2HPO4/NaH2PO4
PH: 7.2
Temperature: 22
uniprot:O14684 [Reduced glutathione]

Prostaglandin_H2 -> Prostaglandin_D2		 (( (Vmax * S) ) / (Km + S)) buffer: 20 mM Na2HPO4/NaH2PO4
PH: 7.2
Temperature: 22

Pathways

pathway id name
BioCyc:META_PWY66-374 C20 prostanoid biosynthesis
BioCyc:HUMAN_PWY66-374 C20 prostanoid biosynthesis
BioCyc:MOUSE_PWY3DJ-35583 biosynthesis of prostaglandins
Reactome:R-HSA-9679506 SARS-CoV Infections
Reactome:R-HSA-9679191 Potential therapeutics for SARS
Reactome:R-HSA-9824446 Viral Infection Pathways
Reactome:R-BTA-1430728 Metabolism
Reactome:R-BTA-556833 Metabolism of lipids
Reactome:R-CFA-1430728 Metabolism
Reactome:R-CFA-556833 Metabolism of lipids
Reactome:R-DME-9006931 Signaling by Nuclear Receptors
Reactome:R-DME-9018519 Estrogen-dependent gene expression
Reactome:R-DME-3371511 HSF1 activation
Reactome:R-DME-8937144 Aryl hydrocarbon receptor signalling
Reactome:R-HSA-211859 Biological oxidations
Reactome:R-HSA-3371571 HSF1-dependent transactivation
Reactome:R-HSA-1643685 Disease
Reactome:R-MMU-1430728 Metabolism
Reactome:R-MMU-556833 Metabolism of lipids
Reactome:R-MMU-211859 Biological oxidations
Reactome:R-MMU-3371568 Attenuation phase
Reactome:R-MMU-3371497 HSP90 chaperone cycle for SHRs
Reactome:R-MMU-162582 Signaling Pathways
Reactome:R-MMU-8939211 ESR-mediated signaling
Reactome:R-RNO-8978868 Fatty acid metabolism
Reactome:R-RNO-2162123 Synthesis of Prostaglandins (PG) and Thromboxanes (TX)
Reactome:R-RNO-211945 Phase I - Functionalization of compounds
Reactome:R-RNO-3371571 HSF1-dependent transactivation
Reactome:R-RNO-162582 Signaling Pathways
Reactome:R-RNO-9006931 Signaling by Nuclear Receptors
Reactome:R-RNO-9018519 Estrogen-dependent gene expression
Reactome:R-BTA-8978868 Fatty acid metabolism
Reactome:R-BTA-2162123 Synthesis of Prostaglandins (PG) and Thromboxanes (TX)
Reactome:R-CFA-8978868 Fatty acid metabolism
Reactome:R-CFA-2162123 Synthesis of Prostaglandins (PG) and Thromboxanes (TX)
Reactome:R-DME-162582 Signaling Pathways
Reactome:R-DME-8939211 ESR-mediated signaling
Reactome:R-DME-8953897 Cellular responses to stimuli
Reactome:R-DME-3371556 Cellular response to heat stress
Reactome:R-DME-1430728 Metabolism
Reactome:R-DME-211945 Phase I - Functionalization of compounds
Reactome:R-HSA-556833 Metabolism of lipids
Reactome:R-HSA-8978868 Fatty acid metabolism
Reactome:R-HSA-2162123 Synthesis of Prostaglandins (PG) and Thromboxanes (TX)
Reactome:R-HSA-9006931 Signaling by Nuclear Receptors
Reactome:R-HSA-9018519 Estrogen-dependent gene expression
Reactome:R-HSA-2262752 Cellular responses to stress
Reactome:R-HSA-3371511 HSF1 activation
Reactome:R-MMU-8978868 Fatty acid metabolism
Reactome:R-MMU-2162123 Synthesis of Prostaglandins (PG) and Thromboxanes (TX)
Reactome:R-MMU-3371571 HSF1-dependent transactivation
Reactome:R-RNO-2142753 Arachidonate metabolism
Reactome:R-RNO-211859 Biological oxidations
Reactome:R-RNO-8953897 Cellular responses to stimuli
Reactome:R-RNO-2262752 Cellular responses to stress
Reactome:R-RNO-3371511 HSF1 activation
Reactome:R-RNO-8939211 ESR-mediated signaling
Reactome:R-DME-2262752 Cellular responses to stress
Reactome:R-DME-3371571 HSF1-dependent transactivation
Reactome:R-DME-3371497 HSP90 chaperone cycle for SHRs
Reactome:R-HSA-211945 Phase I - Functionalization of compounds
Reactome:R-HSA-3371568 Attenuation phase
Reactome:R-HSA-3371497 HSP90 chaperone cycle for SHRs
Reactome:R-MMU-211945 Phase I - Functionalization of compounds
Reactome:R-MMU-3371556 Cellular response to heat stress
Reactome:R-MMU-9006931 Signaling by Nuclear Receptors
Reactome:R-MMU-9018519 Estrogen-dependent gene expression
Reactome:R-RNO-1430728 Metabolism
Reactome:R-RNO-556833 Metabolism of lipids
Reactome:R-RNO-8937144 Aryl hydrocarbon receptor signalling
Reactome:R-RNO-3371568 Attenuation phase
Reactome:R-RNO-3371497 HSP90 chaperone cycle for SHRs
Reactome:R-BTA-2142753 Arachidonate metabolism
Reactome:R-CFA-2142753 Arachidonate metabolism
Reactome:R-DME-3371568 Attenuation phase
Reactome:R-DME-211859 Biological oxidations
Reactome:R-HSA-1430728 Metabolism
Reactome:R-HSA-2142753 Arachidonate metabolism
Reactome:R-HSA-8937144 Aryl hydrocarbon receptor signalling
Reactome:R-HSA-162582 Signaling Pathways
Reactome:R-HSA-8939211 ESR-mediated signaling
Reactome:R-HSA-8953897 Cellular responses to stimuli
Reactome:R-HSA-3371556 Cellular response to heat stress
Reactome:R-HSA-5663205 Infectious disease
Reactome:R-MMU-2142753 Arachidonate metabolism
Reactome:R-MMU-8937144 Aryl hydrocarbon receptor signalling
Reactome:R-MMU-8953897 Cellular responses to stimuli
Reactome:R-MMU-2262752 Cellular responses to stress
Reactome:R-MMU-3371511 HSF1 activation
Reactome:R-RNO-3371556 Cellular response to heat stress
WikiPathways:WP5088 Prostaglandin signaling
PathBank:SMP0000083 Acetylsalicylic Acid Action Pathway
PathBank:SMP0000087 Rofecoxib Action Pathway
PathBank:SMP0000094 Sulindac Action Pathway
PathBank:SMP0000104 Indomethacin Action Pathway
PathBank:SMP0000120 Naproxen Action Pathway
PathBank:SMP0000106 Meloxicam Action Pathway
PathBank:SMP0000693 Antrafenine Action Pathway
PathBank:SMP0000697 Flurbiprofen Action Pathway
PathBank:SMP0000701 Phenylbutazone Action Pathway
PathBank:SMP0000705 Tiaprofenic Acid Action Pathway
PathBank:SMP0000709 Salicylic Acid Action Pathway
PathBank:SMP0000075 Arachidonic Acid Metabolism
PathBank:SMP0000086 Ibuprofen Action Pathway
PathBank:SMP0000093 Diclofenac Action Pathway
PathBank:SMP0000098 Ketorolac Action Pathway
PathBank:SMP0000102 Bromfenac Action Pathway
PathBank:SMP0000114 Nabumetone Action Pathway
PathBank:SMP0000692 Antipyrine Action Pathway
PathBank:SMP0000696 Fenoprofen Action Pathway
PathBank:SMP0000700 Lornoxicam Action Pathway
PathBank:SMP0000704 Tolmetin Action Pathway
PathBank:SMP0000708 Salicylate-Sodium Action Pathway
PathBank:SMP0063595 Arachidonic Acid Metabolism
PathBank:SMP0000077 Piroxicam Action Pathway
PathBank:SMP0000084 Etodolac Action Pathway
PathBank:SMP0000109 Mefenamic Acid Action Pathway
PathBank:SMP0000289 Diflunisal Action Pathway
PathBank:SMP0000116 Valdecoxib Action Pathway
PathBank:SMP0000694 Carprofen Action Pathway
PathBank:SMP0000698 Magnesium Salicylate Action Pathway
PathBank:SMP0000702 Nepafenac Action Pathway
PathBank:SMP0000706 Tenoxicam Action Pathway
PathBank:SMP0000710 Acetaminophen Action Pathway
PathBank:SMP0120510 Leukotriene C4 Synthesis Deficiency
PathBank:SMP0000353 Leukotriene C4 Synthesis Deficiency
PathBank:SMP0000085 Ketoprofen Action Pathway
PathBank:SMP0000096 Celecoxib Action Pathway
PathBank:SMP0000101 Suprofen Action Pathway
PathBank:SMP0000113 Oxaprozin Action Pathway
PathBank:SMP0000695 Etoricoxib Action Pathway
PathBank:SMP0000699 Lumiracoxib Action Pathway
PathBank:SMP0000703 Trisalicylate-Choline Action Pathway
PathBank:SMP0000707 Salsalate Action Pathway