EC: 5.1.3.2

UDP-glucose 4-epimerase (UDP-glucose 4-epimerase)

enzyme lambda metabolic reaction experiment
uniprot:Q14376 [NAD+]

UDP_D_galactose -> UDP_D_glucose		 (( (Vmax * A) ) / (Km + A)) buffer: 0.4 M Glycine, 0.6 mM DTT, 0.18 mg/ml BSA
PH: 8.7
Temperature: 37
uniprot:Q14376 [NAD+]

UDP_D_galactose -> UDP_D_glucose		 (( (Vmax * A) ) / (Km + A)) buffer: 0.4 M Glycine, 0.6 mM DTT, 0.18 mg/ml BSA
PH: 8.7
Temperature: 37
uniprot:Q14376 [NAD+]

UDP_D_galactose -> UDP_D_glucose		 (( (Vmax * A) ) / (Km + A)) buffer: 0.04 M Glycine
PH: 8.7
Temperature: 37
uniprot:Q14376 [NAD+]

UDP_D_galactose -> UDP_D_glucose		 (( (Vmax * A) ) / (Km + A)) buffer: 0.04 M Glycine
PH: 8.7
Temperature: 37
uniprot:Q14376 [NAD+]

UDP_D_galactose -> UDP_D_glucose		 (( (Vmax * A) ) / (Km + A)) buffer: 0.04 M Glycine
PH: 8.7
Temperature: 37
uniprot:P09147 [UDP-glucose 4-epimerase(Enzyme) wildtype]

UDPglucose -> UDPgalactose		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 100 mM glycine-NaOH, 1.25 mM DPN, 2200 units/ml UDP-glucose dehydrogenase
PH: 8.5
Temperature: 27
uniprot:Q58IJ6 [UDP-glucose 4-epimerase(Enzyme) wildtype]

UDP_N_acetyl_D_galactosamine -> UDP_N_acetyl_D_glucosamine		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 20 mM sodium phosphate, 2 mM NAD+
PH: 8
Temperature: 25
uniprot:Q58IJ6 [UDP-glucose 4-epimerase(Enzyme) wildtype]

UDP_N_acetyl_D_glucosamine -> UDP_N_acetyl_D_galactosamine		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 20 mM sodium phosphate, 2 mM NAD+
PH: 8
Temperature: 25
uniprot:Q58IJ6 [UDP-glucose 4-epimerase(Enzyme) wildtype]

UDP_D_galactose -> UDP_D_glucose		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 20 mM sodium phosphate, 2 mM NAD+
PH: 8
Temperature: 25
uniprot:Q58IJ6 [UDP-glucose 4-epimerase(Enzyme) wildtype]

UDP_D_glucose -> UDP_D_galactose		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 20 mM sodium phosphate, 2 mM NAD+
PH: 8
Temperature: 25
uniprot:Q42605 [NAD+]

UDPgalactose -> UDPglucose		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 50 mM Tris/HCl, 125 µg/ml acetylated BSA
PH: 7.4
Temperature: 25
uniprot:Q9C7W7 [NAD+]

UDPgalactose -> UDPglucose		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 50 mM Tris/HCl, 125 µg/ml acetylated BSA
PH: 7.4
Temperature: 25
uniprot:Q8LDN8 [NAD+]

UDPgalactose -> UDPglucose		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 50 mM Tris/HCl, 125 µg/ml acetylated BSA
PH: 7.4
Temperature: 25
uniprot:Q9SN58 [NAD+]

UDPgalactose -> UDPglucose		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 50 mM Tris/HCl, 125 µg/ml acetylated BSA
PH: 7.4
Temperature: 25
uniprot:Q9T0A7 [NAD+]

UDPgalactose -> UDPglucose		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 50 mM Tris/HCl, 125 µg/ml acetylated BSA
PH: 7.4
Temperature: 25
- [UDP-glucose 4-epimerase(Enzyme) wildtype]

UDP_D_glucose -> UDP_D_galactose		 (( (Vmax * S) ) / (Km + S)) buffer: 0.2 M Glycine/NaOH
PH: 9
Temperature: 30
- [UDP-glucose 4-epimerase(Enzyme) wildtype]

UDP_D_galactose -> UDP_D_glucose		 (( (Vmax * S) ) / (Km + S)) buffer: 0.2 M Glycine/NaOH
PH: 9
Temperature: 30
- [UDP-glucose 4-epimerase(Enzyme) wildtype]

UDP_D_galactose -> UDP_D_glucose		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 0.2 M Glycine/NaOH
PH: 9
Temperature: 30
- [UDP-glucose 4-epimerase(Enzyme) wildtype]

UDP_D_glucose -> UDP_D_galactose		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 0.2 M Glycine/NaOH
PH: 9
Temperature: 30
uniprot:Q14376 [UDP-glucose 4-epimerase(Enzyme) wildtype]

UDPgalactose -> UDPglucose		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM Tris/HCl, 4 mM NAD+, 0.02 units/ml UDP-glucose dehydrogenase
PH: 8.8
Temperature: 37
uniprot:Q14376 [UDP-glucose 4-epimerase(Enzyme) mutant N34S]

UDPgalactose -> UDPglucose		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM Tris/HCl, 4 mM NAD+, 0.02 units/ml UDP-glucose dehydrogenase
PH: 8.8
Temperature: 37
uniprot:Q14376 [UDP-glucose 4-epimerase(Enzyme) mutant L313M]

UDPgalactose -> UDPglucose		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM Tris/HCl, 4 mM NAD+, 0.02 units/ml UDP-glucose dehydrogenase
PH: 8.8
Temperature: 37
uniprot:Q14376 [UDP-glucose 4-epimerase(Enzyme) mutant G319E]

UDPgalactose -> UDPglucose		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM Tris/HCl, 4 mM NAD+, 0.02 units/ml UDP-glucose dehydrogenase
PH: 8.8
Temperature: 37
uniprot:Q14376 [UDP-glucose 4-epimerase(Enzyme) mutant G90E]

UDPgalactose -> UDPglucose		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM Tris/HCl, 4 mM NAD+, 0.02 units/ml UDP-glucose dehydrogenase
PH: 8.8
Temperature: 37
uniprot:Q14376 [UDP-glucose 4-epimerase(Enzyme) mutant V94M]

UDPgalactose -> UDPglucose		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM Tris/HCl, 4 mM NAD+, 0.02 units/ml UDP-glucose dehydrogenase
PH: 8.8
Temperature: 37
uniprot:Q14376 [UDP-glucose 4-epimerase(Enzyme) mutant K257R]

UDPgalactose -> UDPglucose		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM Tris/HCl, 4 mM NAD+, 0.02 units/ml UDP-glucose dehydrogenase
PH: 8.8
Temperature: 37
uniprot:Q14376 [UDP-glucose 4-epimerase(Enzyme) mutant R335H]

UDPgalactose -> UDPglucose		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM Tris/HCl, 4 mM NAD+, 0.02 units/ml UDP-glucose dehydrogenase
PH: 8.8
Temperature: 37
uniprot:Q14376 [UDP-glucose 4-epimerase(Enzyme) mutant L183P]

UDPgalactose -> UDPglucose		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM Tris/HCl, 4 mM NAD+, 0.02 units/ml UDP-glucose dehydrogenase
PH: 8.8
Temperature: 37
uniprot:Q14376 [UDP-glucose 4-epimerase(Enzyme) mutant D103G]

UDPgalactose -> UDPglucose		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM Tris/HCl, 4 mM NAD+, 0.02 units/ml UDP-glucose dehydrogenase
PH: 8.8
Temperature: 37
uniprot:Q3T105 [NAD+]

UDPgalactose -> UDPglucose		 (( (V * M) ) / (M + Km)) buffer: 100 mM glycylglycine
PH: 8.5
Temperature: 24
uniprot:Q3T105 [NAD+]

UDPgalactose -> UDPglucose		 (( (V * M) ) / (M + Km)) buffer: 100 mM glycylglycine
PH: 8.5
Temperature: 24
uniprot:Q3T105 [NAD+]

UDPgalactose -> UDPglucose		 (( (V * M) ) / (M + Km)) buffer: 100 mM glycylglycine
PH: 8.5
Temperature: 24
uniprot:Q3T105 [UDP-glucose 4-epimerase(Enzyme) wildtype]

UDPgalactose -> UDPglucose		 (( (V * S) ) / (S + Km)) buffer: 100 mM glycylglycine, 1 mM NAD+, 32 mU/ml UDP-glucose dehydrogenase
PH: 8.5
Temperature: 24
uniprot:Q3T105 [UDP-glucose 4-epimerase(Enzyme) wildtype]

UDPgalactose -> UDPglucose		 (( (V * S) ) / (S + Km)) buffer: 100 mM glycylglycine, 1 mM NAD+, 32 mU/ml UDP-glucose dehydrogenase
PH: 8.5
Temperature: 24
uniprot:P09147 [UDP-glucose 4-epimerase(Enzyme) wildtype]

UDPgalactose -> UDPglucose		 (( (Vmax * A) ) / (Km + A)) buffer: 120 mM Glycine
PH: 8.7
Temperature: 25
uniprot:Q14376 [6-Bromo-2-hydroxy-1-naphthalenecarboxaldehyde-O-uridine-oxime]

UDPgalactose -> UDPglucose		 (( (V * S) ) / (( (Km * (1 + (I / Ki))) ) + S)) buffer: 125 mM potassium bicinate, 1.25 mM NAD+, 0.160 U/ml UDPglucose dehydrogenase
PH: 8.5
Temperature: 36
uniprot:Q14376 [UDP-glucose 4-epimerase(Enzyme) wildtype]

UDPgalactose -> UDPglucose		 (( (V * S) ) / (Km + S)) buffer: 125 mM potassium bicinate, 1.25 mM NAD+, 0.160 U/ml UDPglucose dehydrogenase
PH: 8.5
Temperature: 36
uniprot:P21977 [UDP-glucose 4-epimerase(Enzyme) wildtype]

UDPgalactose -> UDPglucose		 (( (V * S) ) / (Km + S)) buffer: 125 mM potassium bicinate, 1.25 mM NAD+, 0.160 U/ml UDPglucose dehydrogenase
PH: 8.5
Temperature: 36

Pathways

pathway id name
BioCyc:META_PWY-6397 mAGP
BioCyc:META_PWY-3821 D-galactose detoxification
BioCyc:META_PWY-5114 UDP-sugars interconversion
BioCyc:META_PWY66-422 D-galactose degradation V (Leloir pathway)
BioCyc:META_PWY-6317 D-galactose degradation I (Leloir pathway)
BioCyc:META_PWY-7328 superpathway of UDP-glucose-derived O-antigen building blocks biosynthesis
BioCyc:ARA_PWY-7344 UDP-D-galactose biosynthesis
BioCyc:ARA_PWY-5114 UDP-sugars interconversion
BioCyc:ARA_PWY-3821 D-galactose degradation III
BioCyc:MTBH37RV_PWY-6317 D-galactose degradation I (Leloir pathway)
BioCyc:MTBH37RV_PWY-6397 mycolyl-arabinogalactan-peptidoglycan complex biosynthesis
BioCyc:MOUSE_PWY-6317 galactose degradation I (Leloir pathway)
BioCyc:BSUB_PWY-6317 D-galactose degradation I (Leloir pathway)
BioCyc:ECOL316407_GALACTMETAB-PWY galactose degradation I (Leloir pathway)
BioCyc:ECOL316407_COLANSYN-PWY colanic acid building blocks biosynthesis
BioCyc:CORYNE_PWY-7344 UDP-D-galactose biosynthesis
BioCyc:HUMAN_PWY66-422 D-galactose degradation V (Leloir pathway)
BioCyc:META_PWY-5512 UDP-N-acetyl-D-galactosamine biosynthesis I
BioCyc:ECO_PWY-7344 UDP-α-D-galactose biosynthesis
BioCyc:YEAST_PWY66-422 D-galactose degradation V (Leloir pathway)
BioCyc:YEAST_PWY-7344 UDP-D-galactose biosynthesis
BioCyc:ARA_PWY-63 UDP-L-arabinose biosynthesis I (from UDP-xylose)
BioCyc:MTBH37RV_PWY-6404 superpathway of mycolyl-arabinogalactan-peptidoglycan complex biosynthesis
BioCyc:MOUSE_PWY3DJ-193 Leloir pathway
BioCyc:MOUSE_COLANSYN-PWY colanic acid building blocks biosynthesis
BioCyc:ECOL316407_PWY-7344 UDP-D-galactose biosynthesis
BioCyc:CORYNE_PWY-6317 D-galactose degradation I (Leloir pathway)
BioCyc:META_PWY-7820 teichuronic acid biosynthesis (B. subtilis 168)
BioCyc:META_PWY-6404 superpathway of mycolyl-arabinogalactan-peptidoglycan complex biosynthesis
BioCyc:META_COLANSYN-PWY colanic acid building blocks biosynthesis
BioCyc:META_PWY-7344 UDP-α-D-galactose biosynthesis
BioCyc:ECO_COLANSYN-PWY colanic acid building blocks biosynthesis
BioCyc:ARA_PWY-6527 stachyose degradation
BioCyc:MTBH37RV_PWY-7344 UDP-α-D-galactose biosynthesis
BioCyc:MOUSE_GALACTMETAB-PWY UDP-galactose biosynthesis (salvage pathway from galactose using UDP-glucose)
BioCyc:MOUSE_PWY-5514 UDP-N-acetyl-D-galactosamine biosynthesis II
BioCyc:BSUB_PWY-5512 UDP-N-acetyl-D-galactosamine biosynthesis I
BioCyc:CORYNE_PWY-7622 UDP-galactofuranose biosynthesis
BioCyc:HUMAN_PWY-5512 UDP-N-acetyl-D-galactosamine biosynthesis I
BioCyc:HUMAN_PWY-5514 UDP-N-acetyl-D-galactosamine biosynthesis II
BioCyc:ECO_GALACTMETAB-PWY galactose degradation I (Leloir pathway)
BioCyc:ARA_PWY-6317 D-galactose degradation I (Leloir pathway)
BioCyc:MTBH37RV_PWY-7622 UDP-α-D-galactofuranose biosynthesis
BioCyc:MTBH37RV_PWY-5512 UDP-N-acetyl-D-galactosamine biosynthesis I
PathBank:SMP0000841 Galactose Metabolism
PathBank:SMP0000903 Galactose Degradation/Leloir Pathway
PlantCyc:ARA_PWY-5114 UDP-sugars interconversion
PlantCyc:ARA_PWY-6527 stachyose degradation
PlantCyc:ARA_PWY-7344 UDP-α-D-galactose biosynthesis
PlantCyc:ARA_PWY-6317 D-galactose degradation I (Leloir pathway)
PlantCyc:PLANT_PWY-7344 UDP-α-D-galactose biosynthesis
PlantCyc:ARA_PWY-3821 D-galactose detoxification
PlantCyc:ARA_PWY-63 UDP-β-L-arabinose biosynthesis I (from UDP-α-D-xylose)
PlantCyc:PLANT_PWY-6317 D-galactose degradation I (Leloir pathway)
PlantCyc:PLANT_PWY-3821 D-galactose detoxification
Reactome:R-DME-71387 Carbohydrate metabolism
Reactome:R-DME-70370 Galactose catabolism
Reactome:R-HSA-1643685 Disease
Reactome:R-HSA-5609977 Defective GALE causes EDG
Reactome:R-MMU-1430728 Metabolism
Reactome:R-SCE-71387 Carbohydrate metabolism
Reactome:R-SPO-1430728 Metabolism
Reactome:R-SPO-70370 Galactose catabolism
Reactome:R-CEL-71387 Carbohydrate metabolism
Reactome:R-CEL-70370 Galactose catabolism
Reactome:R-DDI-71387 Carbohydrate metabolism
Reactome:R-DME-1430728 Metabolism
Reactome:R-HSA-71387 Carbohydrate metabolism
Reactome:R-HSA-3781865 Diseases of glycosylation
Reactome:R-SCE-1430728 Metabolism
Reactome:R-SCE-70370 Galactose catabolism
Reactome:R-HSA-70370 Galactose catabolism
Reactome:R-HSA-5668914 Diseases of metabolism
Reactome:R-MMU-71387 Carbohydrate metabolism
Reactome:R-MMU-70370 Galactose catabolism
Reactome:R-RNO-1430728 Metabolism
Reactome:R-SPO-71387 Carbohydrate metabolism
Reactome:R-CEL-1430728 Metabolism
Reactome:R-DDI-1430728 Metabolism
Reactome:R-DDI-70370 Galactose catabolism
Reactome:R-HSA-1430728 Metabolism
Reactome:R-HSA-5609975 Diseases associated with glycosylation precursor biosynthesis
Reactome:R-RNO-71387 Carbohydrate metabolism
Reactome:R-RNO-70370 Galactose catabolism
PathBank:SMP0000496 Galactosemia III
PathBank:SMP0002319 Leloir Pathway
PathBank:SMP0002331 Amino Sugar and Nucleotide Sugar Metabolism
PathBank:SMP0087302 Nucleotide Sugars Metabolism
PathBank:SMP0120781 Galactosemia III
PathBank:SMP0000043 Galactose Metabolism
PathBank:SMP0000182 Galactosemia
PathBank:SMP0000495 Galactosemia II (GALK)
PathBank:SMP0120780 Galactosemia II (GALK)
PathBank:SMP0000010 Nucleotide Sugars Metabolism
PathBank:SMP0087320 Galactose Metabolism
PathBank:SMP0087384 Nucleotide Sugars Metabolism
PathBank:SMP0120699 Galactosemia
PathBank:SMP0012059 D-Galactose Degradation (Leloir pathway)