teichuronic acid biosynthesis (B. subtilis 168)

Barreras M, Salinas SR, Abdian PL, Kampel MA, Ielpi L. Structure and Mechanism of GumK, a Membrane-associated Glucuronosyltransferase. Journal of Biological Chemistry. 2008 Sep;283(36):25027–35. doi: 10.1074/jbc.m801227200.; Barreras M, Bianchet MA, Ielpi L. Crystallization and preliminary crystallographic characterization of GumK, a membrane-associated glucuronosyltransferase from Xanthomonas campestris required for xanthan polysaccharide synthesis. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2006 Sep 01;62(Pt 9):880–3. PMID: 16946469; PMCID: PMC2242869.; Soldo B, Lazarevic V, Pagni M, Karamata D. Teichuronic acid operon of Bacillus subtilis 168. Molecular Microbiology. 1999 Feb;31(3):795–805. doi: 10.1046/j.1365-2958.1999.01218.x.; Ward JB. Teichoic and teichuronic acids: biosynthesis, assembly, and location. Microbiol Rev. 1981 Jun;45(2):211–43. doi: 10.1128/mr.45.2.211-243.1981.

Metabolites

H+

Formula: H (1.0078246)

CAS ID: 12408-02-5

H2O

Formula: H2O (18.0105642)

CAS ID: 7732-18-5

UDP-alpha-D-glucose(2-)

Formula: C15H22N2O17P2 (564.0393702)

CAS ID:

NADH(2-)

Formula: C21H27N7O14P2 (663.1091182000001)

CAS ID:

NAD(1-)

Formula: C21H26N7O14P2 (662.1012936000001)

CAS ID: 53-84-9

UDP(3-)

Formula: C9H11N2O12P2 (400.9787246)

CAS ID: 86527-70-0

beta-D-GlcA-(1->3)-alpha-D-GalNAc-(1->6)-alpha-D-GalNAc-diphospho-ditrans,octacis-undecaprenol

Formula: C77H123N2O23P2-3 (1505.7991447999998)

CAS ID:

beta-D-glucuronosyl-(1->4)-beta-D-glucuronosyl-(1->3)-N-acetyl-alpha-D-galactosaminyl-(1->6)-N-acetyl-alpha-D-galactosaminyl-diphospho-ditrans,octacis-undecaprenol

Formula: C83H130N2O29P2-4 (1680.8234069999999)

CAS ID:

alpha-D-GalNAc-(1->6)-alpha-D-GalNAc-diphospho-ditrans,octacis-undecaprenol

Formula: C71H116N2O17P2-2 (1330.7748826)

CAS ID:



Enzyme

EC Number name full name note
2.4.-.-
1.1.1.22 UDP-glucose 6-dehydrogenase UDP-alpha-D-glucose:NAD+ 6-oxidoreductase
2.7.8.40 UDP-N-acetylgalactosamine-undecaprenyl-phosphate N-acetylgalactosaminephosphotransferase UDP-N-acetyl-alpha-D-galactosamine:ditrans,octacis-undecaprenyl phosphate N-acetyl-D-glucosaminephosphotransferase
5.1.3.2 UDP-glucose 4-epimerase UDP-glucose 4-epimerase
5.1.3.7 UDP-N-acetylglucosamine 4-epimerase UDP-N-acetyl-D-glucosamine 4-epimerase


Proteins

Protein ID name full name
O32267 tuaH Putative teichuronic acid biosynthesis glycosyltransferase TuaH
O32268 tuaG Putative teichuronic acid biosynthesis glycosyltransferase TuaG
O32271 tuaD UDP-glucose 6-dehydrogenase TuaD
O32272 tuaC Putative teichuronic acid biosynthesis glycosyltransferase TuaC
O32274 tuaA Putative undecaprenyl-phosphate N-acetylgalactosaminyl 1-phosphate transferase
P55180 galE UDP-glucose 4-epimerase
P96499 tagV Polyisoprenyl-teichoic acid--peptidoglycan teichoic acid transferase TagV
Q02115 tagU Polyisoprenyl-teichoic acid--peptidoglycan teichoic acid transferase TagU
Q7WY78 tagT Polyisoprenyl-teichoic acid--peptidoglycan teichoic acid transferase TagT