EC: 1.11.1.9

glutathione peroxidase (glutathione:hydrogen-peroxide oxidoreductase)

enzyme lambda metabolic reaction experiment
uniprot:P17695 [glutathione peroxidase(Enzyme) wildtype GRX2]

2 Reduced_glutathione + Cumene_hydroperoxide -> H2O + 2_Phenyl_2_propanol + Oxidized_glutathione		 (( (Vmax * S) ) / (Km + S)) buffer: 0.1 mM Tris-HCl, 6 µg/ml Glr1 glutathione reductase, 0.4 mM NADPH
PH: 7.4
Temperature: 36
uniprot:P25373 [glutathione peroxidase(Enzyme) wildtype GRX1]

H2O2 + 2 Reduced_glutathione -> 2 H2O + Oxidized_glutathione		 (( (Vmax * S) ) / (Km + S)) buffer: 0.1 mM Tris-HCl, 6 µg/ml Glr1 glutathione reductase, 0.4 mM NADPH
PH: 7.4
Temperature: 36
uniprot:P17695 [glutathione peroxidase(Enzyme) wildtype GRX2]

H2O2 + 2 Reduced_glutathione -> 2 H2O + Oxidized_glutathione		 (( (Vmax * S) ) / (Km + S)) buffer: 0.1 mM Tris-HCl, 6 µg/ml Glr1 glutathione reductase, 0.4 mM NADPH
PH: 7.4
Temperature: 36
uniprot:P25373 [glutathione peroxidase(Enzyme) wildtype GRX1]

2 Reduced_glutathione + Cumene_hydroperoxide -> Oxidized_glutathione + 2_Phenyl_2_propanol + H2O		 (( (Vmax * S) ) / (Km + S)) buffer: 0.1 mM Tris-HCl, 6 µg/ml Glr1 glutathione reductase, 0.4 mM NADPH
PH: 7.4
Temperature: 36
uniprot:P17695 [glutathione peroxidase(Enzyme) wildtype GRX2]

Reduced_glutathione + t_Butylhydroperoxide -> t_Butanol + Oxidized_glutathione + H2O		 (( (Vmax * S) ) / (Km + S)) buffer: 0.1 mM Tris-HCl, 6 µg/ml Glr1 glutathione reductase, 0.4 mM NADPH
PH: 7.4
Temperature: 36
uniprot:P25373 [glutathione peroxidase(Enzyme) wildtype GRX1]

t_Butylhydroperoxide + Reduced_glutathione -> Oxidized_glutathione + H2O + t_Butanol		 (( (Vmax * S) ) / (Km + S)) buffer: 0.1 mM Tris-HCl, 6 µg/ml Glr1 glutathione reductase, 0.4 mM NADPH
PH: 7.4
Temperature: 36
uniprot:P11352 [glutathione peroxidase(Enzyme) wildtype GPX1]

H2O2 + 2 Reduced_glutathione -> Oxidized_glutathione + 2 H2O		 (( (Vmax * A) ) / (Km + A)) buffer: 0.1 M potassium phosphate
PH: 7
Temperature: 25
uniprot:P11352 [glutathione peroxidase(Enzyme) wildtype GPX1]

H2O2 + 2 Reduced_glutathione -> Oxidized_glutathione + 2 H2O		 (( (Vmax * A) ) / (Km + A)) buffer: 0.1 M potassium phosphate
PH: 7
Temperature: 25
uniprot:P11352 [glutathione peroxidase(Enzyme) wildtype GPX1]

Reduced_glutathione + t_Butylhydroperoxide -> t_Butanol + H2O + Oxidized_glutathione		 (( (Vmax * A) ) / (Km + A)) buffer: 0.1 M potassium phosphate
PH: 7
Temperature: 25
uniprot:P11352 [glutathione peroxidase(Enzyme) wildtype GPX1]

t_Butylhydroperoxide + Reduced_glutathione -> H2O + t_Butanol + Oxidized_glutathione		 (( (Vmax * A) ) / (Km + A)) buffer: 0.1 M potassium phosphate
PH: 7
Temperature: 25
uniprot:P25373 [glutathione peroxidase(Enzyme) wildtype Grx1]

2 Reduced_glutathione + H2O2 -> Oxidized_glutathione + 2 H2O		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 0.1 M Tris-HCl, 0.4 mM NADPH, 6 µg/ml Glutathione reductase 1
PH: 7.4
Temperature: 36
uniprot:P17695 [glutathione peroxidase(Enzyme) wildtype Grx2]

H2O2 + 2 Reduced_glutathione -> Oxidized_glutathione + 2 H2O		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 0.1 M Tris-HCl, 0.4 mM NADPH, 6 µg/ml Glutathione reductase 1
PH: 7.4
Temperature: 36
uniprot:P25373 [glutathione peroxidase(Enzyme) wildtype Grx1]

2 Reduced_glutathione + Cumene_hydroperoxide -> 2_Phenyl_2_propanol + Oxidized_glutathione + H2O		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 0.1 M Tris-HCl, 0.4 mM NADPH, 6 µg/ml Glutathione reductase 1
PH: 7.4
Temperature: 36
uniprot:P25373 [glutathione peroxidase(Enzyme) wildtype Grx1]

t_Butylhydroperoxide + Reduced_glutathione -> H2O + t_Butanol + Oxidized_glutathione		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 0.1 M Tris-HCl, 0.4 mM NADPH, 6 µg/ml Glutathione reductase 1
PH: 7.4
Temperature: 36
uniprot:P17695 [glutathione peroxidase(Enzyme) wildtype Grx2]

t_Butylhydroperoxide + Reduced_glutathione -> H2O + t_Butanol + Oxidized_glutathione		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 0.1 M Tris-HCl, 0.4 mM NADPH, 6 µg/ml Glutathione reductase 1
PH: 7.4
Temperature: 36
uniprot:P17695 [glutathione peroxidase(Enzyme) wildtype Grx2]

2 Reduced_glutathione + Cumene_hydroperoxide -> Oxidized_glutathione + H2O + 2_Phenyl_2_propanol		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 0.1 M Tris-HCl, 0.4 mM NADPH, 6 µg/ml Glutathione reductase 1
PH: 7.4
Temperature: 36
- [NADPH]

H2O2 + 2 Reduced_glutathione -> 2 H2O + Oxidized_glutathione		 (( (Vmax * S) ) / (Km + S)) buffer: 20 mM MOPS, 120 mM KCl, 1 mM EGTA, 0.02% Triton X-100, 0.5-1 U/ml glutathione reductase, 0.15-0.2 mM NADPH
PH: 7.2
Temperature: 37
- [NADPH]

H2O2 + 2 Reduced_glutathione -> Oxidized_glutathione + 2 H2O		 (( (Vmax * S) ) / (Km + S)) buffer: 20 mM MOPS, 120 mM KCl, 1 mM EGTA, 0.02% Triton X-100, 0.5-1 U/ml glutathione reductase, 0.15-0.2 mM NADPH
PH: 7.2
Temperature: 37
- [NADPH]

H2O2 + 2 Reduced_glutathione -> Oxidized_glutathione + 2 H2O		 (( (Vmax * S) ) / (Km + S)) buffer: 20 mM MOPS, 120 mM KCl, 1 mM EGTA, 0.02% Triton X-100, 0.5-1 U/ml glutathione reductase, 0.15-0.2 mM NADPH
PH: 7.2
Temperature: 37
- [NADPH]

2 Reduced_glutathione + H2O2 -> 2 H2O + Oxidized_glutathione		 (( (Vmax * S) ) / (Km + S)) buffer: 20 mM MOPS, 120 mM KCl, 1 mM EGTA, 0.02% Triton X-100, 0.5-1 U/ml glutathione reductase, 0.15-0.2 mM NADPH
PH: 7.2
Temperature: 37
- [NADPH]

H2O2 + 2 Reduced_glutathione -> Oxidized_glutathione + 2 H2O		 (( (Vmax * S) ) / (Km + S)) buffer: 20 mM MOPS, 120 mM KCl, 1 mM EGTA, 0.02% Triton X-100, 0.5-1 U/ml glutathione reductase, 0.15-0.2 mM NADPH
PH: 7.2
Temperature: 37
- [NADPH]

2 Reduced_glutathione + H2O2 -> Oxidized_glutathione + 2 H2O		 (( (Vmax * S) ) / (Km + S)) buffer: 20 mM MOPS, 120 mM KCl, 1 mM EGTA, 0.02% Triton X-100, 0.5-1 U/ml glutathione reductase, 0.15-0.2 mM NADPH
PH: 7.2
Temperature: 37
- [glutathione peroxidase(Enzyme) wildtype GPx-1 and GPx-4]

Cumene_hydroperoxide + 2 Reduced_glutathione -> 2_Phenyl_2_propanol + Oxidized_glutathione + H2O		 (( (Vmax * S) ) / (Km + S)) buffer: 20 mM MOPS, 120 mM KCl, 1 mM EGTA, 0.02% Triton X-100, 0.5-1 U/ml glutathione reductase, 0.15-0.2 mM NADPH
PH: 7.2
Temperature: 37
- [glutathione peroxidase(Enzyme) wildtype GPx-1 and GPx-4]

Cumene_hydroperoxide + 2 Reduced_glutathione -> 2_Phenyl_2_propanol + H2O + Oxidized_glutathione		 (( (Vmax * S) ) / (Km + S)) buffer: 20 mM MOPS, 120 mM KCl, 1 mM EGTA, 0.02% Triton X-100, 0.5-1 U/ml glutathione reductase, 0.15-0.2 mM NADPH
PH: 7.2
Temperature: 37
- [glutathione peroxidase(Enzyme) wildtype GPx-1 and GPx-4]

2 Reduced_glutathione + Cumene_hydroperoxide -> H2O + 2_Phenyl_2_propanol + Oxidized_glutathione		 (( (Vmax * S) ) / (Km + S)) buffer: 20 mM MOPS, 120 mM KCl, 1 mM EGTA, 0.02% Triton X-100, 0.5-1 U/ml glutathione reductase, 0.15-0.2 mM NADPH
PH: 7.2
Temperature: 37
- [glutathione peroxidase(Enzyme) wildtype GPx-1 and GPx-4]

Reduced_glutathione + t_Butylhydroperoxide -> H2O + t_Butanol + Oxidized_glutathione		 (( (Vmax * S) ) / (Km + S)) buffer: 20 mM MOPS, 120 mM KCl, 1 mM EGTA, 0.02% Triton X-100, 0.5-1 U/ml glutathione reductase, 0.15-0.2 mM NADPH
PH: 7.2
Temperature: 37
- [glutathione peroxidase(Enzyme) wildtype GPx-1 and GPx-4]

Reduced_glutathione + t_Butylhydroperoxide -> t_Butanol + Oxidized_glutathione + H2O		 (( (Vmax * S) ) / (Km + S)) buffer: 20 mM MOPS, 120 mM KCl, 1 mM EGTA, 0.02% Triton X-100, 0.5-1 U/ml glutathione reductase, 0.15-0.2 mM NADPH
PH: 7.2
Temperature: 37
- [glutathione peroxidase(Enzyme) wildtype GPx-1 and GPx-4]

t_Butylhydroperoxide + Reduced_glutathione -> Oxidized_glutathione + H2O + t_Butanol		 (( (Vmax * S) ) / (Km + S)) buffer: 20 mM MOPS, 120 mM KCl, 1 mM EGTA, 0.02% Triton X-100, 0.5-1 U/ml glutathione reductase, 0.15-0.2 mM NADPH
PH: 7.2
Temperature: 37

Pathways

pathway id name
BioCyc:META_PWY-4081 glutathione-peroxide redox reactions
BioCyc:YEAST_PWY-6842-1 glutathione-mediated detoxification II
BioCyc:YEAST_PWY-4081 glutathione redox reactions I
BioCyc:ARA_PWY-4081 glutathione-peroxide redox reactions
BioCyc:MOUSE_PWY-4081 glutathione redox reactions I
BioCyc:HUMAN_DETOX1-PWY-1 reactive oxygen species degradation
BioCyc:HUMAN_PWY-4081 glutathione redox reactions I
BioCyc:META_DETOX1-PWY-1 reactive oxygen species degradation
BioCyc:ARA_DETOX1-PWY-1 reactive oxygen species degradation
PlantCyc:ARA_DETOX1-PWY-1 reactive oxygen species degradation
PlantCyc:ARA_PWY-4081 glutathione-peroxide redox reactions
Reactome:R-RNO-9711123 Cellular response to chemical stress
Reactome:R-BTA-9711123 Cellular response to chemical stress
Reactome:R-MMU-9711123 Cellular response to chemical stress
Reactome:R-HSA-9711123 Cellular response to chemical stress
Reactome:R-BTA-1430728 Metabolism
Reactome:R-BTA-556833 Metabolism of lipids
Reactome:R-BTA-9018896 Biosynthesis of E-series 18(S)-resolvins
Reactome:R-BTA-9020265 Biosynthesis of aspirin-triggered D-series resolvins
Reactome:R-BTA-8953897 Cellular responses to stimuli
Reactome:R-BTA-2262752 Cellular responses to stress
Reactome:R-HSA-425410 Metal ion SLC transporters
Reactome:R-HSA-1643685 Disease
Reactome:R-HSA-5619102 SLC transporter disorders
Reactome:R-HSA-5619049 Defective SLC40A1 causes hemochromatosis 4 (HFE4) (macrophages)
Reactome:R-HSA-74160 Gene expression (Transcription)
Reactome:R-HSA-73857 RNA Polymerase II Transcription
Reactome:R-MMU-1430728 Metabolism
Reactome:R-MMU-556833 Metabolism of lipids
Reactome:R-MMU-9018679 Biosynthesis of EPA-derived SPMs
Reactome:R-MMU-9018676 Biosynthesis of D-series resolvins
Reactome:R-MMU-425410 Metal ion SLC transporters
Reactome:R-MMU-3299685 Detoxification of Reactive Oxygen Species
Reactome:R-MMU-74160 Gene expression (Transcription)
Reactome:R-MMU-73857 RNA Polymerase II Transcription
Reactome:R-MMU-3700989 Transcriptional Regulation by TP53
Reactome:R-RNO-9018677 Biosynthesis of DHA-derived SPMs
Reactome:R-RNO-382551 Transport of small molecules
Reactome:R-RNO-425407 SLC-mediated transmembrane transport
Reactome:R-RNO-917937 Iron uptake and transport
Reactome:R-SSC-9018677 Biosynthesis of DHA-derived SPMs
Reactome:R-BTA-9018679 Biosynthesis of EPA-derived SPMs
Reactome:R-BTA-9018676 Biosynthesis of D-series resolvins
Reactome:R-HSA-2262752 Cellular responses to stress
Reactome:R-HSA-5619115 Disorders of transmembrane transporters
Reactome:R-MMU-9018677 Biosynthesis of DHA-derived SPMs
Reactome:R-RNO-9018678 Biosynthesis of specialized proresolving mediators (SPMs)
Reactome:R-RNO-9023661 Biosynthesis of E-series 18(R)-resolvins
Reactome:R-RNO-8953897 Cellular responses to stimuli
Reactome:R-RNO-2262752 Cellular responses to stress
Reactome:R-SSC-1430728 Metabolism
Reactome:R-SSC-556833 Metabolism of lipids
Reactome:R-SSC-9018678 Biosynthesis of specialized proresolving mediators (SPMs)
Reactome:R-SSC-9023661 Biosynthesis of E-series 18(R)-resolvins
Reactome:R-BTA-9018678 Biosynthesis of specialized proresolving mediators (SPMs)
Reactome:R-BTA-9023661 Biosynthesis of E-series 18(R)-resolvins
Reactome:R-HSA-3299685 Detoxification of Reactive Oxygen Species
Reactome:R-HSA-212436 Generic Transcription Pathway
Reactome:R-HSA-3700989 Transcriptional Regulation by TP53
Reactome:R-MMU-9018896 Biosynthesis of E-series 18(S)-resolvins
Reactome:R-MMU-9020265 Biosynthesis of aspirin-triggered D-series resolvins
Reactome:R-MMU-212436 Generic Transcription Pathway
Reactome:R-MMU-5628897 TP53 Regulates Metabolic Genes
Reactome:R-RNO-1430728 Metabolism
Reactome:R-RNO-556833 Metabolism of lipids
Reactome:R-RNO-9018679 Biosynthesis of EPA-derived SPMs
Reactome:R-RNO-9018676 Biosynthesis of D-series resolvins
Reactome:R-RNO-74160 Gene expression (Transcription)
Reactome:R-RNO-73857 RNA Polymerase II Transcription
Reactome:R-RNO-3700989 Transcriptional Regulation by TP53
Reactome:R-RNO-3299685 Detoxification of Reactive Oxygen Species
Reactome:R-SSC-9018679 Biosynthesis of EPA-derived SPMs
Reactome:R-SSC-9018676 Biosynthesis of D-series resolvins
Reactome:R-BTA-9018677 Biosynthesis of DHA-derived SPMs
Reactome:R-BTA-3299685 Detoxification of Reactive Oxygen Species
Reactome:R-HSA-382551 Transport of small molecules
Reactome:R-HSA-425407 SLC-mediated transmembrane transport
Reactome:R-HSA-917937 Iron uptake and transport
Reactome:R-HSA-8953897 Cellular responses to stimuli
Reactome:R-HSA-5628897 TP53 Regulates Metabolic Genes
Reactome:R-MMU-9018678 Biosynthesis of specialized proresolving mediators (SPMs)
Reactome:R-MMU-9023661 Biosynthesis of E-series 18(R)-resolvins
Reactome:R-MMU-382551 Transport of small molecules
Reactome:R-MMU-425407 SLC-mediated transmembrane transport
Reactome:R-MMU-917937 Iron uptake and transport
Reactome:R-MMU-8953897 Cellular responses to stimuli
Reactome:R-MMU-2262752 Cellular responses to stress
Reactome:R-RNO-9018896 Biosynthesis of E-series 18(S)-resolvins
Reactome:R-RNO-9020265 Biosynthesis of aspirin-triggered D-series resolvins
Reactome:R-RNO-212436 Generic Transcription Pathway
Reactome:R-RNO-5628897 TP53 Regulates Metabolic Genes
Reactome:R-RNO-425410 Metal ion SLC transporters
Reactome:R-SSC-9018896 Biosynthesis of E-series 18(S)-resolvins
Reactome:R-SSC-9020265 Biosynthesis of aspirin-triggered D-series resolvins
PathBank:SMP0120480 gamma-Glutamyltransferase Deficiency
PathBank:SMP0120565 5-Oxoprolinase Deficiency
PathBank:SMP0000015 Glutathione Metabolism
PathBank:SMP0000083 Acetylsalicylic Acid Action Pathway
PathBank:SMP0000087 Rofecoxib Action Pathway
PathBank:SMP0000094 Sulindac Action Pathway
PathBank:SMP0000104 Indomethacin Action Pathway
PathBank:SMP0000120 Naproxen Action Pathway
PathBank:SMP0000106 Meloxicam Action Pathway
PathBank:SMP0000500 5-Oxoprolinase Deficiency
PathBank:SMP0000693 Antrafenine Action Pathway
PathBank:SMP0000697 Flurbiprofen Action Pathway
PathBank:SMP0000701 Phenylbutazone Action Pathway
PathBank:SMP0000705 Tiaprofenic Acid Action Pathway
PathBank:SMP0000709 Salicylic Acid Action Pathway
PathBank:SMP0000075 Arachidonic Acid Metabolism
PathBank:SMP0000086 Ibuprofen Action Pathway
PathBank:SMP0000093 Diclofenac Action Pathway
PathBank:SMP0000098 Ketorolac Action Pathway
PathBank:SMP0000102 Bromfenac Action Pathway
PathBank:SMP0000114 Nabumetone Action Pathway
PathBank:SMP0000692 Antipyrine Action Pathway
PathBank:SMP0000696 Fenoprofen Action Pathway
PathBank:SMP0000700 Lornoxicam Action Pathway
PathBank:SMP0000704 Tolmetin Action Pathway
PathBank:SMP0000708 Salicylate-Sodium Action Pathway
PathBank:SMP0063595 Arachidonic Acid Metabolism
PathBank:SMP0120485 Glutathione Synthetase Deficiency
PathBank:SMP0120566 gamma-Glutamyltranspeptidase Deficiency
PathBank:SMP0000337 Glutathione Synthetase Deficiency
PathBank:SMP0000077 Piroxicam Action Pathway
PathBank:SMP0000084 Etodolac Action Pathway
PathBank:SMP0000109 Mefenamic Acid Action Pathway
PathBank:SMP0000289 Diflunisal Action Pathway
PathBank:SMP0000116 Valdecoxib Action Pathway
PathBank:SMP0000501 gamma-Glutamyltranspeptidase Deficiency
PathBank:SMP0000694 Carprofen Action Pathway
PathBank:SMP0000698 Magnesium Salicylate Action Pathway
PathBank:SMP0000702 Nepafenac Action Pathway
PathBank:SMP0000706 Tenoxicam Action Pathway
PathBank:SMP0000710 Acetaminophen Action Pathway
PathBank:SMP0002409 TOR Signalling Pathway: Rapamycin-Based Repression
PathBank:SMP0120446 5-Oxoprolinuria
PathBank:SMP0120510 Leukotriene C4 Synthesis Deficiency
PathBank:SMP0000143 5-Oxoprolinuria
PathBank:SMP0000183 gamma-Glutamyltransferase Deficiency
PathBank:SMP0000353 Leukotriene C4 Synthesis Deficiency
PathBank:SMP0000085 Ketoprofen Action Pathway
PathBank:SMP0000096 Celecoxib Action Pathway
PathBank:SMP0000101 Suprofen Action Pathway
PathBank:SMP0000113 Oxaprozin Action Pathway
PathBank:SMP0000695 Etoricoxib Action Pathway
PathBank:SMP0000699 Lumiracoxib Action Pathway
PathBank:SMP0000703 Trisalicylate-Choline Action Pathway
PathBank:SMP0000707 Salsalate Action Pathway
PathBank:SMP0087427 Glutathione Metabolism
PathBank:SMP0063622 Glutathione Metabolism