uniprot:Q15126 [phosphomevalonate kinase(Enzyme) wildtype his-tagged]
(R)_5_Diphosphomevalonate + ADP -> ATP + (R)_5_Phosphomevalonate |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 100 mM MOPS, 200 mM KCl, 1 MM DTT, 10 mM MgCl2, coupled assay: ATP production was coupled to the reduction of NADP+ with hexokinase/glucose-6-phosphate dehydrogenase (3 U per assay)
PH: 7
Temperature: 30 |
uniprot:Q15126 [phosphomevalonate kinase(Enzyme) wildtype his-tagged]
ADP + (R)_5_Diphosphomevalonate -> (R)_5_Phosphomevalonate + ATP |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 100 mM MOPS, 200 mM KCl, 1 MM DTT, 10 mM MgCl2, coupled assay: ATP production was coupled to the reduction of NADP+ with hexokinase/glucose-6-phosphate dehydrogenase (3 U per assay)
PH: 7
Temperature: 30 |
uniprot:Q15126 [phosphomevalonate kinase(Enzyme) wildtype]
ATP + (R)_5_Phosphomevalonate -> ADP + (R)_5_Diphosphomevalonate |
(( (V * B) ) / (Km + B)) |
|
buffer: 50 mM MOPS-KOH, 6 mM MgCl2, 10 mM KF, 1 mM phosphoenolpyruvate, 0.2 mM NADH, 10 U/ml pyruvate kinase, 10 U/ml lactate dehydrogenase
PH: 7
Temperature: 25 |
uniprot:Q15126 [phosphomevalonate kinase(Enzyme) wildtype]
(R)_5_Phosphomevalonate + ATP -> ADP + (R)_5_Diphosphomevalonate |
(( (V * A) ) / (Km + A)) |
|
buffer: 50 mM MOPS-KOH, 6 mM MgCl2, 10 mM KF, 1 mM phosphoenolpyruvate, 0.2 mM NADH, 10 U/ml pyruvate kinase, 10 U/ml lactate dehydrogenase
PH: 7
Temperature: 25 |
- [phosphomevalonate kinase(Enzyme) wildtype]
(R)_5_Phosphomevalonate + ATP -> (R)_5_Diphosphomevalonate + ADP |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 50 mM Tris/HCl, 20 mM MgCl2, 10 mM DTT, 5 mM p-Coumarate
PH: 7.4
Temperature: 37 |
- [phosphomevalonate kinase(Enzyme) wildtype]
(R)_5_Phosphomevalonate + ATP -> (R)_5_Diphosphomevalonate + ADP |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 50 mM Tris/HCl, 20 mM MgCl2, 10 mM DTT, 5 mM p-Coumarate
PH: 7.4
Temperature: 37 |
uniprot:Q97UL6 [phosphomevalonate kinase(Enzyme) wildtype]
ATP + (R)_5_Phosphomevalonate -> (R)_5_Diphosphomevalonate + ADP |
(( (Vmax * A) ) / (Km + A)) |
|
buffer: 73 mM potassium phosphate, 10 U/ml pyruvate kinase, 10 U/ml lactate dehydrogenase, 5 mM phosphoenolpyruvate, 0.16 mM NADH, 5 mM MgCl2
PH: 7
Temperature: 37 |
uniprot:Q837E1 [phosphomevalonate kinase(Enzyme) wildtype]
(R)_5_Phosphomevalonate + ATP -> (R)_5_Diphosphomevalonate + ADP |
(( (Vmax * A) ) / (Km + A)) |
|
buffer: 50 mM Tris, 75 mM KCl, 5 mM MgCl2, 5 mM MnCl2, 2.5 mM phosphoenolpyruvate, ~0.16 mM NADH, 10 mM DTT, 5 units L-lactate dehydrogenase, 10 units pyruvate kinase
PH: 8
Temperature: 25 |
uniprot:Q837E1 [phosphomevalonate kinase(Enzyme) wildtype]
(R)_5_Phosphomevalonate + ATP -> ADP + (R)_5_Diphosphomevalonate |
(( (Vmax * A) ) / (Km + A)) |
|
buffer: 50 mM Tris, 75 mM KCl, 5 mM MgCl2, 5 mM MnCl2, 2.5 mM phosphoenolpyruvate, ~0.16 mM NADH, 10 mM DTT, 5 units L-lactate dehydrogenase, 10 units pyruvate kinase
PH: 8
Temperature: 25 |
uniprot:Q15126 [phosphomevalonate kinase(Enzyme) wildtype his-tagged]
(R)_5_Phosphomevalonate + ATP -> (R)_5_Diphosphomevalonate + ADP |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 100 mM MOPS, 200 mM KCl, 1 MM DTT, 10 mM MgCl2, coupled assay: couple ADP production to oxidation of NADH with pyruvate kinase/lactate dehydrogenase (3 U per assay)
PH: 7
Temperature: 30 |
uniprot:Q15126 [phosphomevalonate kinase(Enzyme) mutant K17M his-tagged]
(R)_5_Phosphomevalonate + ATP -> ADP + (R)_5_Diphosphomevalonate |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 100 mM MOPS, 200 mM KCl, 1 MM DTT, 10 mM MgCl2, coupled assay: couple ADP production to oxidation of NADH with pyruvate kinase/lactate dehydrogenase (3 U per assay)
PH: 7
Temperature: 30 |
uniprot:Q15126 [phosphomevalonate kinase(Enzyme) mutant K17M his-tagged]
(R)_5_Diphosphomevalonate + ADP -> (R)_5_Phosphomevalonate + ATP |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 100 mM MOPS, 200 mM KCl, 1 MM DTT, 10 mM MgCl2, coupled assay: ATP production was coupled to the reduction of NADP+ with hexokinase/glucose-6-phosphate dehydrogenase (3 U per assay)
PH: 7
Temperature: 30 |
uniprot:Q15126 [phosphomevalonate kinase(Enzyme) wildtype his-tagged]
(R)_5_Phosphomevalonate + ATP -> ADP + (R)_5_Diphosphomevalonate |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 100 mM MOPS, 200 mM KCl, 1 MM DTT, 10 mM MgCl2, coupled assay: couple ADP production to oxidation of NADH with pyruvate kinase/lactate dehydrogenase (3 U per assay)
PH: 7
Temperature: 30 |
uniprot:Q15126 [phosphomevalonate kinase(Enzyme) mutant K17M his-tagged]
ATP + (R)_5_Phosphomevalonate -> ADP + (R)_5_Diphosphomevalonate |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 100 mM MOPS, 200 mM KCl, 1 MM DTT, 10 mM MgCl2, coupled assay: couple ADP production to oxidation of NADH with pyruvate kinase/lactate dehydrogenase (3 U per assay)
PH: 7
Temperature: 30 |
uniprot:Q15126 [phosphomevalonate kinase(Enzyme) mutant D23N his-tagged]
ADP + (R)_5_Diphosphomevalonate -> ATP + (R)_5_Phosphomevalonate |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 100 mM MOPS, 200 mM KCl, 1 MM DTT, 10 mM MgCl2, coupled assay: ATP production was coupled to the reduction of NADP+ with hexokinase/glucose-6-phosphate dehydrogenase (3 U per assay)
PH: 7
Temperature: 30 |
uniprot:Q15126 [phosphomevalonate kinase(Enzyme) mutant D23N his-tagged]
ATP + (R)_5_Phosphomevalonate -> (R)_5_Diphosphomevalonate + ADP |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 100 mM MOPS, 200 mM KCl, 1 MM DTT, 10 mM MgCl2, coupled assay: couple ADP production to oxidation of NADH with pyruvate kinase/lactate dehydrogenase (3 U per assay)
PH: 7
Temperature: 30 |
uniprot:Q15126 [phosphomevalonate kinase(Enzyme) mutant D23N his-tagged]
ATP + (R)_5_Phosphomevalonate -> ADP + (R)_5_Diphosphomevalonate |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 100 mM MOPS, 200 mM KCl, 1 MM DTT, 10 mM MgCl2, coupled assay: couple ADP production to oxidation of NADH with pyruvate kinase/lactate dehydrogenase (3 U per assay)
PH: 7
Temperature: 30 |
uniprot:Q15126 [phosphomevalonate kinase(Enzyme) mutant D23N his-tagged]
ADP + (R)_5_Diphosphomevalonate -> ATP + (R)_5_Phosphomevalonate |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 100 mM MOPS, 200 mM KCl, 1 MM DTT, 10 mM MgCl2, coupled assay: ATP production was coupled to the reduction of NADP+ with hexokinase/glucose-6-phosphate dehydrogenase (3 U per assay)
PH: 7
Temperature: 30 |
uniprot:Q15126 [phosphomevalonate kinase(Enzyme) mutant K22M his-tagged]
(R)_5_Diphosphomevalonate + ADP -> (R)_5_Phosphomevalonate + ATP |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 100 mM MOPS, 200 mM KCl, 1 MM DTT, 10 mM MgCl2, coupled assay: ATP production was coupled to the reduction of NADP+ with hexokinase/glucose-6-phosphate dehydrogenase (3 U per assay)
PH: 7
Temperature: 30 |
uniprot:Q15126 [phosphomevalonate kinase(Enzyme) mutant K22B his-tagged]
(R)_5_Phosphomevalonate + ATP -> ADP + (R)_5_Diphosphomevalonate |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 100 mM MOPS, 200 mM KCl, 1 MM DTT, 10 mM MgCl2, coupled assay: couple ADP production to oxidation of NADH with pyruvate kinase/lactate dehydrogenase (3 U per assay)
PH: 7
Temperature: 30 |
uniprot:Q15126 [phosphomevalonate kinase(Enzyme) mutant R18Q his-tagged]
ATP + (R)_5_Phosphomevalonate -> (R)_5_Diphosphomevalonate + ADP |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 100 mM MOPS, 200 mM KCl, 1 MM DTT, 10 mM MgCl2, coupled assay: couple ADP production to oxidation of NADH with pyruvate kinase/lactate dehydrogenase (3 U per assay)
PH: 7
Temperature: 30 |
uniprot:Q15126 [phosphomevalonate kinase(Enzyme) mutant K17M his-tagged]
ADP + (R)_5_Diphosphomevalonate -> ATP + (R)_5_Phosphomevalonate |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 100 mM MOPS, 200 mM KCl, 1 MM DTT, 10 mM MgCl2, coupled assay: ATP production was coupled to the reduction of NADP+ with hexokinase/glucose-6-phosphate dehydrogenase (3 U per assay)
PH: 7
Temperature: 30 |
uniprot:Q15126 [phosphomevalonate kinase(Enzyme) mutant R18Q his-tagged]
ADP + (R)_5_Diphosphomevalonate -> (R)_5_Phosphomevalonate + ATP |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 100 mM MOPS, 200 mM KCl, 1 MM DTT, 10 mM MgCl2, coupled assay: ATP production was coupled to the reduction of NADP+ with hexokinase/glucose-6-phosphate dehydrogenase (3 U per assay)
PH: 7
Temperature: 30 |
uniprot:Q15126 [phosphomevalonate kinase(Enzyme) mutant K19M his-tagged]
ADP + (R)_5_Diphosphomevalonate -> ATP + (R)_5_Phosphomevalonate |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 100 mM MOPS, 200 mM KCl, 1 MM DTT, 10 mM MgCl2, coupled assay: ATP production was coupled to the reduction of NADP+ with hexokinase/glucose-6-phosphate dehydrogenase (3 U per assay)
PH: 7
Temperature: 30 |
uniprot:Q15126 [phosphomevalonate kinase(Enzyme) mutant K19M his-tagged]
ATP + (R)_5_Phosphomevalonate -> (R)_5_Diphosphomevalonate + ADP |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 100 mM MOPS, 200 mM KCl, 1 MM DTT, 10 mM MgCl2, coupled assay: couple ADP production to oxidation of NADH with pyruvate kinase/lactate dehydrogenase (3 U per assay)
PH: 7
Temperature: 30 |
uniprot:Q15126 [phosphomevalonate kinase(Enzyme) mutant R18Q his-tagged]
ATP + (R)_5_Phosphomevalonate -> (R)_5_Diphosphomevalonate + ADP |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 100 mM MOPS, 200 mM KCl, 1 MM DTT, 10 mM MgCl2, coupled assay: couple ADP production to oxidation of NADH with pyruvate kinase/lactate dehydrogenase (3 U per assay)
PH: 7
Temperature: 30 |
uniprot:Q15126 [phosphomevalonate kinase(Enzyme) mutant K19M his-tagged]
ATP + (R)_5_Phosphomevalonate -> ADP + (R)_5_Diphosphomevalonate |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 100 mM MOPS, 200 mM KCl, 1 MM DTT, 10 mM MgCl2, coupled assay: couple ADP production to oxidation of NADH with pyruvate kinase/lactate dehydrogenase (3 U per assay)
PH: 7
Temperature: 30 |
uniprot:Q15126 [phosphomevalonate kinase(Enzyme) mutant K19M his-tagged]
(R)_5_Diphosphomevalonate + ADP -> (R)_5_Phosphomevalonate + ATP |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 100 mM MOPS, 200 mM KCl, 1 MM DTT, 10 mM MgCl2, coupled assay: ATP production was coupled to the reduction of NADP+ with hexokinase/glucose-6-phosphate dehydrogenase (3 U per assay)
PH: 7
Temperature: 30 |
uniprot:Q15126 [phosphomevalonate kinase(Enzyme) mutant R18Q his-tagged]
ADP + (R)_5_Diphosphomevalonate -> ATP + (R)_5_Phosphomevalonate |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 100 mM MOPS, 200 mM KCl, 1 MM DTT, 10 mM MgCl2, coupled assay: ATP production was coupled to the reduction of NADP+ with hexokinase/glucose-6-phosphate dehydrogenase (3 U per assay)
PH: 7
Temperature: 30 |