EC: 1.5.1.2

pyrroline-5-carboxylate reductase (L-proline:NAD(P)+ 5-oxidoreductase)

enzyme lambda metabolic reaction experiment
uniprot:Q96C36 [pyrroline-5-carboxylate reductase(Enzyme) wildtype]

H+ + (S)_1_Pyrroline_5_carboxylate + NADH -> NAD+ + L_Proline		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM Potassium phosphate
PH: 6.8
Temperature: 37
uniprot:Q96C36 [pyrroline-5-carboxylate reductase(Enzyme) wildtype]

(S)_1_Pyrroline_5_carboxylate + H+ + NADH -> L_Proline + NAD+		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM Potassium phosphate
PH: 6.8
Temperature: 37
uniprot:Q96C36 [pyrroline-5-carboxylate reductase(Enzyme) wildtype]

H+ + NADH + (S)_1_Pyrroline_5_carboxylate -> NAD+ + L_Proline		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM Potassium phosphate
PH: 6.8
Temperature: 37
uniprot:Q96C36 [pyrroline-5-carboxylate reductase(Enzyme) wildtype]

NADPH + H+ + (S)_1_Pyrroline_5_carboxylate -> L_Proline + NADP+		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM Potassium phosphate
PH: 6.8
Temperature: 37
uniprot:Q96C36 [pyrroline-5-carboxylate reductase(Enzyme) wildtype]

(S)_1_Pyrroline_5_carboxylate + H+ + NADPH -> NADP+ + L_Proline		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM Potassium phosphate
PH: 6.8
Temperature: 37
uniprot:Q96C36 [NADP+]

H+ + (S)_1_Pyrroline_5_carboxylate + NADH -> NAD+ + L_Proline		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM Potassium phosphate
PH: 6.8
Temperature: 37
uniprot:Q96C36 [pyrroline-5-carboxylate reductase(Enzyme) wildtype]

H+ + (S)_1_Pyrroline_5_carboxylate + NADPH -> L_Proline + NADP+		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM Potassium phosphate
PH: 6.8
Temperature: 37
uniprot:Q96C36 [pyrroline-5-carboxylate reductase(Enzyme) wildtype]

H+ + NADPH + (S)_1_Pyrroline_5_carboxylate -> NADP+ + L_Proline		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM Potassium phosphate
PH: 6.8
Temperature: 37
- [pyrroline-5-carboxylate reductase(Enzyme) wildtype]

NADH + (S)_1_Pyrroline_5_carboxylate + H+ -> L_Proline + NAD+		 (( (Vmax * S) ) / (Km_app + S)) buffer: 0.1 M Potassium phosphate
PH: 6.8
Temperature: 25
- [pyrroline-5-carboxylate reductase(Enzyme) wildtype]

(S)_1_Pyrroline_5_carboxylate + H+ + NADH -> NAD+ + L_Proline		 (( (Vmax * S) ) / (Km_app + S)) buffer: 0.1 M Potassium phosphate
PH: 6.8
Temperature: 25
- [pyrroline-5-carboxylate reductase(Enzyme) wildtype]

NADPH + (S)_1_Pyrroline_5_carboxylate + H+ -> L_Proline + NADP+		 (( (Vmax * S) ) / (Km_app + S)) buffer: 0.1 M Potassium phosphate
PH: 6.8
Temperature: 25
- [pyrroline-5-carboxylate reductase(Enzyme) wildtype]

(S)_1_Pyrroline_5_carboxylate + NADPH + H+ -> NADP+ + L_Proline		 (( (Vmax * S) ) / (Km_app + S)) buffer: 0.1 M Potassium phosphate
PH: 6.8
Temperature: 25
uniprot:Q04708 [pyrroline-5-carboxylate reductase(Enzyme) wildtype]

NADH + H+ + (S)_1_Pyrroline_5_carboxylate -> L_Proline + NAD+		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Tricine-KOH, 1 mM DTT
PH: 7.5
Temperature: 36
uniprot:Q04708 [pyrroline-5-carboxylate reductase(Enzyme) wildtype]

(S)_1_Pyrroline_5_carboxylate + NADH + H+ -> NAD+ + L_Proline		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Tricine-KOH, 1 mM DTT
PH: 7.5
Temperature: 36
uniprot:Q04708 [pyrroline-5-carboxylate reductase(Enzyme) wildtype]

(S)_1_Pyrroline_5_carboxylate + NADPH + H+ -> NADP+ + L_Proline		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Tricine-KOH, 1 mM DTT
PH: 7.5
Temperature: 36
uniprot:Q04708 [pyrroline-5-carboxylate reductase(Enzyme) wildtype]

H+ + NADPH + (S)_1_Pyrroline_5_carboxylate -> NADP+ + L_Proline		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Tricine-KOH, 1 mM DTT
PH: 7.5
Temperature: 36
uniprot:P54904 [pyrroline-5-carboxylate reductase(Enzyme) wildtype]

NADPH + H+ + (S)_1_Pyrroline_5_carboxylate -> NADP+ + L_Proline		 (( (Vmax * S) ) / (Km + S)) buffer: 100 mM Tris-HCl, 0.1 mM MgCl2
PH: 7.4
Temperature: 35
uniprot:P54904 [pyrroline-5-carboxylate reductase(Enzyme) wildtype]

(S)_1_Pyrroline_5_carboxylate + NADH + H+ -> NAD+ + L_Proline		 (( (Vmax * S) ) / (Km + S)) buffer: 100 mM Tris-HCl, 0.1 mM MgCl2
PH: 7.4
Temperature: 35
uniprot:P54904 [pyrroline-5-carboxylate reductase(Enzyme) wildtype]

H+ + NADH + (S)_1_Pyrroline_5_carboxylate -> L_Proline + NAD+		 (( (Vmax * S) ) / (Km + S)) buffer: 100 mM Tris-HCl, 0.1 mM MgCl2
PH: 7.4
Temperature: 35
uniprot:P32322 [pyrroline-5-carboxylate reductase(Enzyme) wildtype isoenzyme PYCR1]

Thioproline + NAD+ -> NADH + 1_Thiopyrroline_5_carboxylate + H+		 (( (( (E * kcat) ) * B) ) / (Km + B)) buffer: 300 mM Tris-HCl
PH: 9
Temperature: 25
uniprot:P32322 [pyrroline-5-carboxylate reductase(Enzyme) mutant isoenzyme PYCR1 E221A]

Thioproline + NAD+ -> NADH + 1_Thiopyrroline_5_carboxylate + H+		 (( (( (E * kcat) ) * B) ) / (Km + B)) buffer: 300 mM Tris-HCl
PH: 9
Temperature: 25
uniprot:P32322 [pyrroline-5-carboxylate reductase(Enzyme) mutant isoenzyme PYCR1 E221A]

Thioproline + NADP+ -> 1_Thiopyrroline_5_carboxylate + H+ + NADPH		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 300 mM Tris-HCl
PH: 9
Temperature: 25
uniprot:P32322 [pyrroline-5-carboxylate reductase(Enzyme) wildtype isoenzyme PYCR1]

Thioproline + NADP+ -> NADPH + 1_Thiopyrroline_5_carboxylate + H+		 (( (( (E * kcat) ) * B) ) / (Km + B)) buffer: 300 mM Tris-HCl
PH: 9
Temperature: 25
uniprot:P32322 [pyrroline-5-carboxylate reductase(Enzyme) wildtype isoenzyme PYCR1]

NADP+ + Thioproline -> H+ + 1_Thiopyrroline_5_carboxylate + NADPH		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 300 mM Tris-HCl
PH: 9
Temperature: 25
uniprot:P32322 [pyrroline-5-carboxylate reductase(Enzyme) mutant isoenzyme PYCR1 E221A]

NADP+ + Thioproline -> NADPH + 1_Thiopyrroline_5_carboxylate + H+		 (( (( (E * kcat) ) * B) ) / (Km + B)) buffer: 300 mM Tris-HCl
PH: 9
Temperature: 25

Pathways

pathway id name
BioCyc:HUMAN_PROSYN-PWY proline biosynthesis
BioCyc:HUMAN_PWY66-425 lysine degradation II (pipecolate pathway)
BioCyc:META_ARG-PRO-PWY L-arginine degradation VI (arginase 2 pathway)
BioCyc:MTBH37RV_PROSYN-PWY L-proline biosynthesis I
BioCyc:BSUB_PROSYN-PWY L-proline biosynthesis I
BioCyc:BSUB_PWY-6344 L-ornithine degradation II (Stickland reaction)
BioCyc:YEAST_ARG-PRO-PWY arginine degradation VI (arginase 2 pathway)
BioCyc:MTBCDC1551_PROSYN-PWY L-proline biosynthesis I
BioCyc:MOUSE_PWY-4981 proline biosynthesis II (from arginine)
BioCyc:MOUSE_ARG-PRO-PWY arginine degradation VI (arginase 2 pathway)
BioCyc:ECOL316407_PROSYN-PWY proline biosynthesis I
BioCyc:CORYNE_PROSYN-PWY L-proline biosynthesis I
BioCyc:ECO_PROSYN-PWY L-proline biosynthesis I
BioCyc:MOUSE_PROLINE-SYN2-PWY proline biosynthesis II
BioCyc:BSUB_ARG-PRO-PWY L-arginine degradation VI (arginase 2 pathway)
BioCyc:META_PWY66-425 L-lysine degradation II (L-pipecolate pathway)
BioCyc:META_PROSYN-PWY L-proline biosynthesis I
BioCyc:YEAST_PROSYN-PWY proline biosynthesis
BioCyc:MTBH37RV_PWY-3341 L-proline biosynthesis III
PathBank:SMP0000815 Proline Metabolism
Reactome:R-RNO-8964539 Glutamate and glutamine metabolism
Reactome:R-SCE-8964539 Glutamate and glutamine metabolism
Reactome:R-DRE-8964539 Glutamate and glutamine metabolism
Reactome:R-SPO-8964539 Glutamate and glutamine metabolism
Reactome:R-DDI-8964539 Glutamate and glutamine metabolism
Reactome:R-MMU-8964539 Glutamate and glutamine metabolism
Reactome:R-HSA-8964539 Glutamate and glutamine metabolism
Reactome:R-BTA-8964539 Glutamate and glutamine metabolism
Reactome:R-BTA-1430728 Metabolism
Reactome:R-BTA-71291 Amino acid and derivative metabolism
Reactome:R-DRE-71291 Amino acid and derivative metabolism
Reactome:R-MMU-1430728 Metabolism
Reactome:R-SCE-71291 Amino acid and derivative metabolism
Reactome:R-SPO-1430728 Metabolism
Reactome:R-DDI-71291 Amino acid and derivative metabolism
Reactome:R-SCE-1430728 Metabolism
Reactome:R-SPO-71291 Amino acid and derivative metabolism
Reactome:R-DRE-1430728 Metabolism
Reactome:R-HSA-71291 Amino acid and derivative metabolism
Reactome:R-MMU-71291 Amino acid and derivative metabolism
Reactome:R-RNO-1430728 Metabolism
Reactome:R-RNO-71291 Amino acid and derivative metabolism
Reactome:R-DDI-1430728 Metabolism
Reactome:R-HSA-1430728 Metabolism
WikiPathways:WP4595 Urea cycle and associated pathways
WikiPathways:WP497 Urea cycle and metabolism of amino groups
WikiPathways:WP5312 Metabolic pathways of fibroblasts
PathBank:SMP0120710 Guanidinoacetate Methyltransferase Deficiency (GAMT Deficiency)
PathBank:SMP0120758 Ornithine Aminotransferase Deficiency (OAT Deficiency)
PathBank:SMP0120790 Hyperornithinemia-Hyperammonemia-Homocitrullinuria [HHH-syndrome]
PathBank:SMP0120569 Creatine Deficiency, Guanidinoacetate Methyltransferase Deficiency
PathBank:SMP0000020 Arginine and Proline Metabolism
PathBank:SMP0000362 Arginine: Glycine Amidinotransferase Deficiency (AGAT Deficiency)
PathBank:SMP0000188 Guanidinoacetate Methyltransferase Deficiency (GAMT Deficiency)
PathBank:SMP0000504 Creatine Deficiency, Guanidinoacetate Methyltransferase Deficiency
PathBank:SMP0002420 Proline Metabolism
PathBank:SMP0063596 Arginine and Proline Metabolism
PathBank:SMP0120721 Hyperprolinemia Type II
PathBank:SMP0120761 Prolidase Deficiency (PD)
PathBank:SMP0120789 Hyperornithinemia with Gyrate Atrophy (HOGA)
PathBank:SMP0120499 Hyperprolinemia Type I
PathBank:SMP0120539 Prolinemia Type II
PathBank:SMP0120572 L-Arginine:Glycine Amidinotransferase Deficiency
PathBank:SMP0000207 Prolidase Deficiency (PD)
PathBank:SMP0000208 Prolinemia Type II
PathBank:SMP0000507 L-Arginine:Glycine Amidinotransferase Deficiency
PathBank:SMP0002416 Arginine Metabolism
PathBank:SMP0120788 Creatine Deficiency, Guanidinoacetate Methyltransferase Deficiency
PathBank:SMP0120501 Hyperprolinemia Type II
PathBank:SMP0120541 Prolidase Deficiency (PD)
PathBank:SMP0120570 Hyperornithinemia with Gyrate Atrophy (HOGA)
PathBank:SMP0000360 Hyperprolinemia Type II
PathBank:SMP0000363 Ornithine Aminotransferase Deficiency (OAT Deficiency)
PathBank:SMP0000505 Hyperornithinemia with Gyrate Atrophy (HOGA)
PathBank:SMP0002297 Proline Metabolism
PathBank:SMP0120675 Arginine: Glycine Amidinotransferase Deficiency (AGAT Deficiency)
PathBank:SMP0120719 Hyperprolinemia Type I
PathBank:SMP0120759 Prolinemia Type II
PathBank:SMP0120791 L-Arginine:Glycine Amidinotransferase Deficiency
PathBank:SMP0120454 Arginine: Glycine Amidinotransferase Deficiency (AGAT Deficiency)
PathBank:SMP0120490 Guanidinoacetate Methyltransferase Deficiency (GAMT Deficiency)
PathBank:SMP0120538 Ornithine Aminotransferase Deficiency (OAT Deficiency)
PathBank:SMP0120571 Hyperornithinemia-Hyperammonemia-Homocitrullinuria [HHH-syndrome]
PathBank:SMP0000361 Hyperprolinemia Type I
PathBank:SMP0000506 Hyperornithinemia-Hyperammonemia-Homocitrullinuria [HHH-syndrome]
PathBank:SMP0087178 Arginine and Proline Metabolism
PathBank:SMP0087283 Arginine and Proline Metabolism