EC: 1.14.14.18

heme oxygenase (biliverdin-producing) (protoheme,NADPH-hemoprotein reductase:oxygen oxidoreductase (alpha-methene-oxidizing, hydroxylating))

enzyme lambda metabolic reaction experiment
uniprot:P14791 [heme oxygenase(Enzyme) wildtype]

3 O2 + Heme + 3 NADPH -> Biliverdin + 3 H2O + Fe2+ + 3 NADP+ + Carbon_monoxide		 (( (Vmax * A) ) / (Km + A)) buffer: 70 mM Potassium phosphate, 5 mM Deferoxamine, 1.5 units/ml Biliverdin reductase, 0.6 units/ml NADPH-cytochrome reductase, 4000 U/ml Catalase, 0.5 mg/ml Emulgen, 1 mg/ml Egg phosphatidylcholine
PH: 7.4
Temperature: 37
uniprot:P14791 [heme oxygenase(Enzyme) wildtype]

3 NADPH + Heme + 3 O2 -> Carbon_monoxide + 3 H2O + Biliverdin + Fe2+ + 3 NADP+		 (( (Vmax * A) ) / (Km + A)) buffer: 70 mM Potassium phosphate, 5 mM Deferoxamine, 1.5 units/ml Biliverdin reductase, 0.6 units/ml NADPH-cytochrome reductase, 4000 U/ml Catalase, 0.5 mg/ml Emulgen, 1 mg/ml Egg phosphatidylcholine, 25 µM Bovine serum albumin
PH: 7.4
Temperature: 37
uniprot:P14791 [heme oxygenase(Enzyme) wildtype]

3 O2 + 3 NADPH + Heme -> Carbon_monoxide + 3 NADP+ + Fe2+ + 3 H2O + Biliverdin		 (( (Vmax * A) ) / (Km + A)) buffer: 70 mM Potassium phosphate, 5 mM Deferoxamine, 1.5 units/ml Biliverdin reductase, 0.6 units/ml NADPH-cytochrome reductase, 4000 U/ml Catalase, 0.5 mg/ml Emulgen, 1 mg/ml Egg phosphatidylcholine, 25 µM Bovine serum albumin
PH: 7.4
Temperature: 37
uniprot:Q5E9F2 [heme oxygenase(Enzyme) wildtype]

Heme + 3 NADPH + 3 O2 -> 3 NADP+ + Carbon_monoxide + Fe2+ + Biliverdin + 3 H2O		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM Potassium phosphate, 0.65 units/ml NADPH-cytochrome c reductase
PH: 7.5
Temperature: 37
- [heme oxygenase(Enzyme) wildtype]

3 NADPH + Heme + 3 O2 -> Carbon_monoxide + Biliverdin + 3 NADP+ + Fe2+ + 3 H2O		 (( (Vmax * A) ) / (Km + A)) buffer: 0.1 mM Potassium phosphate, 200 µg/ml Biliverdin reductase, 10-12 µg/ml NADPH-cytochrome c reductase, 0.8 mM NADP+, 4 mM Glucose 6-phosphate, 0.2 units/ml Glucose 6-phosphate dehydrogenase
PH: 7.5
Temperature: 36
uniprot:P06762 [heme oxygenase(Enzyme) wildtype]

Heme + 3 NADPH + 3 O2 -> Biliverdin + 3 H2O + 3 NADP+ + Carbon_monoxide + Fe2+		 (( (Vmax * A) ) / (Km + A)) buffer: 90 mM Potassium phosphate, 20 µg/ml NADPH-cytochrome c reductase
PH: 7.4
Temperature: 37
uniprot:P23711 [heme oxygenase(Enzyme) wildtype HO-2]

Hematin + 3 O2 + 3 NADPH -> 3 NADP+ + Biliverdin + Fe2+ + 3 H2O + Carbon_monoxide		 (( (Vmax * A) ) / (Km + A)) buffer: 0.1 M Potassium phosphate, NADPH-cytochrome c reductase, 1 unit/ml Biliverdin reductase
PH: 7.4
Temperature: 37
uniprot:P06762 [heme oxygenase(Enzyme) wildtype HO-1]

Hematin + 3 O2 + 3 NADPH -> Biliverdin + 3 NADP+ + Carbon_monoxide + Fe2+ + 3 H2O		 (( (Vmax * A) ) / (Km + A)) buffer: 0.1 M Potassium phosphate, NADPH-cytochrome c reductase, 1 unit/ml Biliverdin reductase
PH: 7.4
Temperature: 37
uniprot:P06762 [heme oxygenase(Enzyme) wildtype]

Hematoheme + 3 NADPH + 3 O2 -> 3 H2O + 3 NADP+ + Carbon_monoxide + Hematobiliverdin + Fe2+		 (( (Vmax * A) ) / (Km + A)) buffer: 90 mM Potassium phosphate, 20 µg/ml NADPH-cytochrome c reductase
PH: 7.4
Temperature: 37
uniprot:Q5E9F2 [heme oxygenase(Enzyme) wildtype]

3 O2 + 3 NADPH + Heme_c -> Fe2+ + Biliverdin_c + 3 NADP+ + Carbon_monoxide + 3 H2O		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM Potassium phosphate, 0.65 units/ml NADPH-cytochrome c reductase
PH: 7.5
Temperature: 37

Pathways

pathway id name
BioCyc:META_PWY-7845 heme degradation II
BioCyc:META_PWY-7578 phycoviolobilin biosynthesis
BioCyc:META_PWY-5917 phycocyanobilin biosynthesis
BioCyc:META_PWY-5874 heme degradation I
BioCyc:HUMAN_PWY-5874 heme degradation I
BioCyc:META_PWY-7170 phytochromobilin biosynthesis
BioCyc:MOUSE_PWY-5874 heme degradation
Reactome:R-HSA-9660826 Purinergic signaling in leishmaniasis infection
Reactome:R-RNO-9711123 Cellular response to chemical stress
Reactome:R-HSA-9707587 Regulation of HMOX1 expression and activity
Reactome:R-HSA-9664424 Cell recruitment (pro-inflammatory response)
Reactome:R-SSC-9707587 Regulation of HMOX1 expression and activity
Reactome:R-BTA-9707587 Regulation of HMOX1 expression and activity
Reactome:R-MMU-9707587 Regulation of HMOX1 expression and activity
Reactome:R-HSA-9707564 Cytoprotection by HMOX1
Reactome:R-HSA-9818027 NFE2L2 regulating anti-oxidant/detoxification enzymes
Reactome:R-HSA-9658195 Leishmania infection
Reactome:R-SSC-9711123 Cellular response to chemical stress
Reactome:R-RNO-9707564 Cytoprotection by HMOX1
Reactome:R-BTA-9711123 Cellular response to chemical stress
Reactome:R-MMU-9711123 Cellular response to chemical stress
Reactome:R-HSA-9707616 Heme signaling
Reactome:R-HSA-9755511 KEAP1-NFE2L2 pathway
Reactome:R-SSC-9707564 Cytoprotection by HMOX1
Reactome:R-RNO-9707587 Regulation of HMOX1 expression and activity
Reactome:R-BTA-9707564 Cytoprotection by HMOX1
Reactome:R-MMU-9707564 Cytoprotection by HMOX1
Reactome:R-HSA-9711123 Cellular response to chemical stress
Reactome:R-HSA-9759194 Nuclear events mediated by NFE2L2
Reactome:R-HSA-9824443 Parasitic Infection Pathways
Reactome:R-BTA-8953897 Cellular responses to stimuli
Reactome:R-BTA-2262752 Cellular responses to stress
Reactome:R-HSA-1643685 Disease
Reactome:R-HSA-6785807 Interleukin-4 and Interleukin-13 signaling
Reactome:R-SSC-8953897 Cellular responses to stimuli
Reactome:R-SSC-2262752 Cellular responses to stress
Reactome:R-HSA-2262752 Cellular responses to stress
Reactome:R-HSA-168643 Nucleotide-binding domain, leucine rich repeat containing receptor (NLR) signaling pathways
Reactome:R-HSA-449147 Signaling by Interleukins
Reactome:R-RNO-8953897 Cellular responses to stimuli
Reactome:R-RNO-2262752 Cellular responses to stress
Reactome:R-HSA-168256 Immune System
Reactome:R-HSA-168249 Innate Immune System
Reactome:R-HSA-622312 Inflammasomes
Reactome:R-HSA-8953897 Cellular responses to stimuli
Reactome:R-HSA-5663205 Infectious disease
Reactome:R-HSA-844456 The NLRP3 inflammasome
Reactome:R-HSA-1280215 Cytokine Signaling in Immune system
Reactome:R-MMU-8953897 Cellular responses to stimuli
Reactome:R-MMU-2262752 Cellular responses to stress
PathBank:SMP0120492 Hereditary Coproporphyria (HCP)
PathBank:SMP0120544 Porphyria Variegata (PV)
PathBank:SMP0000024 Porphyrin Metabolism
PathBank:SMP0120447 Acute Intermittent Porphyria
PathBank:SMP0000345 Congenital Erythropoietic Porphyria (CEP) or Gunther Disease
PathBank:SMP0063655 Porphyrin Metabolism
PathBank:SMP0120469 Congenital Erythropoietic Porphyria (CEP) or Gunther Disease
PathBank:SMP0000344 Acute Intermittent Porphyria
PathBank:SMP0000346 Porphyria Variegata (PV)
PathBank:SMP0000342 Hereditary Coproporphyria (HCP)