EC: 2.7.7.4

sulfate adenylyltransferase (ATP:sulfate adenylyltransferase)

enzyme lambda metabolic reaction experiment
uniprot:O43252 [sulfate adenylyltransferase(Enzyme) wildtype isoenzyme PAPSS1]

Sulfate + ATP -> Diphosphate + Adenylylsulfate		 (( (( (E * kcat) ) * B) ) / (Kb + B)) buffer: 45 mM Tris/HCl, 15 mM KCl, 4.5 mM MgCl2, 0.9 mM EDTA, 13.5 mM dithiothreitol
PH: 8
Temperature: 37
uniprot:O43252 [sulfate adenylyltransferase(Enzyme) wildtype isoenzyme PAPSS1]

ATP + Sulfate -> Diphosphate + Adenylylsulfate		 (( (( (E * kcat) ) * A) ) / (Ka + A)) buffer: 45 mM Tris/HCl, 15 mM KCl, 4.5 mM MgCl2, 0.9 mM EDTA, 13.5 mM dithiothreitol
PH: 8
Temperature: 37
uniprot:O95340 [sulfate adenylyltransferase(Enzyme) wildtype isoenzyme PAPSS2a]

Sulfate + ATP -> Diphosphate + Adenylylsulfate		 (( (( (E * kcat) ) * A) ) / (Ka + A)) buffer: 45 mM Tris/HCl, 15 mM KCl, 4.5 mM MgCl2, 0.9 mM EDTA, 13.5 mM dithiothreitol
PH: 8
Temperature: 37
uniprot:O95340 [sulfate adenylyltransferase(Enzyme) wildtype isoenzyme PAPSS2b]

Sulfate + ATP -> Diphosphate + Adenylylsulfate		 (( (( (E * kcat) ) * B) ) / (Kb + B)) buffer: 45 mM Tris/HCl, 15 mM KCl, 4.5 mM MgCl2, 0.9 mM EDTA, 13.5 mM dithiothreitol
PH: 8
Temperature: 37
uniprot:O95340 [sulfate adenylyltransferase(Enzyme) wildtype isoenzyme PAPSS2b]

ATP + Sulfate -> Adenylylsulfate + Diphosphate		 (( (( (E * kcat) ) * A) ) / (Ka + A)) buffer: 45 mM Tris/HCl, 15 mM KCl, 4.5 mM MgCl2, 0.9 mM EDTA, 13.5 mM dithiothreitol
PH: 8
Temperature: 37
uniprot:O95340 [sulfate adenylyltransferase(Enzyme) wildtype isoenzyme PAPSS2a]

ATP + Sulfate -> Adenylylsulfate + Diphosphate		 (( (( (E * kcat) ) * B) ) / (Kb + B)) buffer: 45 mM Tris/HCl, 15 mM KCl, 4.5 mM MgCl2, 0.9 mM EDTA, 13.5 mM dithiothreitol
PH: 8
Temperature: 37
uniprot:O43252 [sulfate adenylyltransferase(Enzyme) wildtype PAPS synthetase isoform 1]

ATP + Sulfate -> Diphosphate + Adenylylsulfate		 (( (Vmax * A) ) / (KmA + A)) buffer: 50 mM Tris-HCl, 100 mM NaCl, 5 mM DTT
PH: 8
Temperature: 30
uniprot:O43252 [sulfate adenylyltransferase(Enzyme) wildtype PAPS synthetase isoform 1]

ATP + Sulfate -> Adenylylsulfate + Diphosphate		 (( (Vmax * A) ) / (KmA + A)) buffer: 50 mM Tris-HCl, 100 mM NaCl, 5 mM DTT
PH: 8
Temperature: 30
uniprot:O43252 [sulfate adenylyltransferase(Enzyme) wildtype PAPS synthetase isoform 1]

ATP + Sulfate -> Diphosphate + Adenylylsulfate		 (( (Vmax * B) ) / (KmB + B)) buffer: 50 mM Tris-HCl, 100 mM NaCl, 5 mM DTT
PH: 8
Temperature: 30
uniprot:O43252 [sulfate adenylyltransferase(Enzyme) wildtype PAPS synthetase isoform 1]

ATP + Sulfate -> Adenylylsulfate + Diphosphate		 (( (( (Vmax * A) ) * B) ) / (( (( (( (( (Kia * KmB) ) + (KmA * B)) ) + (KmB * A)) ) + (A * B)) ) + (( (( (Kia * KmB) ) * Q) ) / Kiq))) buffer: 50 mM Tris-HCl, 100 mM NaCl, 5 mM DTT
PH: 8
Temperature: 30
uniprot:O43252 [sulfate adenylyltransferase(Enzyme) wildtype PAPS synthetase isoform 1]

Adenylylsulfate + Diphosphate -> Sulfate + ATP		 (( (Vmax_r * Q) ) / (KmQ + Q)) buffer: 50 mM Tris-HCl, 100 mM NaCl, 1 mM glucose, 5 mM MgCl2, 0.3 mM NADP+
PH: 8
Temperature: 30
uniprot:O43252 [sulfate adenylyltransferase(Enzyme) wildtype PAPS synthetase isoform 1]

Diphosphate + Adenylylsulfate -> Sulfate + ATP		 (( (Vmax_r * P) ) / (KmP + P)) buffer: 50 mM Tris-HCl, 100 mM NaCl, 1 mM glucose, 5 mM MgCl2, 0.3 mM NADP+
PH: 8
Temperature: 30
uniprot:O43252 [sulfate adenylyltransferase(Enzyme) wildtype PAPS synthetase isoform 1]

ATP + Molybdate -> Adenylylmolybdate + Diphosphate		 (( (Vmax * B) ) / (KmB + B)) buffer: 50 mM Tris-HCl, 100 mM NaCl, 5 mM DTT
PH: 8
Temperature: 30
uniprot:O43252 [sulfate adenylyltransferase(Enzyme) wildtype PAPS synthetase isoform 1]

ATP + Molybdate -> Adenylylmolybdate + Diphosphate		 (( (Vmax * A) ) / (KmA + A)) buffer: 50 mM Tris-HCl, 100 mM NaCl, 5 mM DTT
PH: 8
Temperature: 30
uniprot:O43252 [sulfate adenylyltransferase(Enzyme) wildtype PAPS synthetase isoform 1]

ATP + Molybdate -> Adenylylmolybdate + Diphosphate		 (( (Vmax * B) ) / (KmB + B)) buffer: 50 mM Tris-HCl, 100 mM NaCl, 5 mM DTT
PH: 8
Temperature: 30
uniprot:O43252 [sulfate adenylyltransferase(Enzyme) wildtype PAPS synthetase isoform 1]

ATP + Molybdate -> Diphosphate + Adenylylmolybdate		 (( (Vmax * A) ) / (KmA + A)) buffer: 50 mM Tris-HCl, 100 mM NaCl, 5 mM DTT
PH: 8
Temperature: 30
uniprot:O43252 [sulfate adenylyltransferase(Enzyme) wildtype PAPS synthetase isoform 1]

Selenate + ATP -> Diphosphate + Adenosine_5'_phosphoselenate		 (( (Vmax * B) ) / (KmB + B)) buffer: 50 mM Tris-HCl, 100 mM NaCl, 5 mM DTT
PH: 8
Temperature: 30
uniprot:O43252 [sulfate adenylyltransferase(Enzyme) wildtype PAPS synthetase isoform 1]

ATP + Selenate -> Diphosphate + Adenosine_5'_phosphoselenate		 (( (Vmax * B) ) / (KmB + B)) buffer: 50 mM Tris-HCl, 100 mM NaCl, 5 mM DTT
PH: 8
Temperature: 30
uniprot:O43252 [sulfate adenylyltransferase(Enzyme) wildtype PAPS synthetase isoform 1]

Selenate + ATP -> Adenosine_5'_phosphoselenate + Diphosphate		 (( (Vmax * A) ) / (KmA + A)) buffer: 50 mM Tris-HCl, 100 mM NaCl, 5 mM DTT
PH: 8
Temperature: 30
uniprot:O43252 [sulfate adenylyltransferase(Enzyme) wildtype PAPS synthetase isoform 1]

Selenate + ATP -> Adenosine_5'_phosphoselenate + Diphosphate		 (( (Vmax * A) ) / (KmA + A)) buffer: 50 mM Tris-HCl, 100 mM NaCl, 5 mM DTT
PH: 8
Temperature: 30
uniprot:O43252 [sulfate adenylyltransferase(Enzyme) wildtype PAPS synthetase isoform 1]

ATP + Arsenate -> Diphosphate + Adenylylarsenate		 (( (Vmax * B) ) / (KmB + B)) buffer: 50 mM Tris-HCl, 100 mM NaCl, 5 mM DTT
PH: 8
Temperature: 30
uniprot:O43252 [sulfate adenylyltransferase(Enzyme) wildtype PAPS synthetase isoform 1]

ATP + Arsenate -> Adenylylarsenate + Diphosphate		 (( (Vmax * B) ) / (KmB + B)) buffer: 50 mM Tris-HCl, 100 mM NaCl, 5 mM DTT
PH: 8
Temperature: 30
uniprot:O43252 [sulfate adenylyltransferase(Enzyme) wildtype PAPS synthetase isoform 1]

Arsenate + ATP -> Adenylylarsenate + Diphosphate		 (( (Vmax * A) ) / (KmA + A)) buffer: 50 mM Tris-HCl, 100 mM NaCl, 5 mM DTT
PH: 8
Temperature: 30
uniprot:O43252 [sulfate adenylyltransferase(Enzyme) wildtype PAPS synthetase isoform 1]

Tungstate + ATP -> Diphosphate + Adenylyltungstate		 (( (Vmax * B) ) / (KmB + B)) buffer: 50 mM Tris-HCl, 100 mM NaCl, 5 mM DTT
PH: 8
Temperature: 30
uniprot:O43252 [sulfate adenylyltransferase(Enzyme) wildtype PAPS synthetase isoform 1]

ATP + Tungstate -> Diphosphate + Adenylyltungstate		 (( (Vmax * A) ) / (KmA + A)) buffer: 50 mM Tris-HCl, 100 mM NaCl, 5 mM DTT
PH: 8
Temperature: 30
uniprot:O43252 [sulfate adenylyltransferase(Enzyme) wildtype PAPS synthetase isoform 1]

ATP + Phosphate -> ADP + Diphosphate		 (( (Vmax * B) ) / (KmB + B)) buffer: 50 mM Tris-HCl, 100 mM NaCl, 5 mM DTT
PH: 8
Temperature: 30
uniprot:O43252 [sulfate adenylyltransferase(Enzyme) wildtype PAPS synthetase isoform 1]

ATP + Phosphate -> ADP + Diphosphate		 (( (Vmax * A) ) / (KmA + A)) buffer: 50 mM Tris-HCl, 100 mM NaCl, 5 mM DTT
PH: 8
Temperature: 30
uniprot:O43252 [sulfate adenylyltransferase(Enzyme) wildtype PAPS synthetase isoform 1]

ATP + Molybdate -> Diphosphate + Adenylylmolybdate		 (( (( (Vmax * A) ) * B) ) / (( (( (( (( (Kia * KmB) ) + (KmA * B)) ) + (KmB * A)) ) + (A * B)) ) + (( (( (Kia * KmB) ) * Q) ) / Kiq))) buffer: 50 mM Tris-HCl, 100 mM NaCl, 5 mM DTT
PH: 8
Temperature: 30
uniprot:O43252 [sulfate adenylyltransferase(Enzyme) wildtype PAPS synthetase isoform 1]

ATP + Arsenate -> Diphosphate + Adenylylarsenate		 (( (Vmax * A) ) / (KmA + A)) buffer: 50 mM Tris-HCl, 100 mM NaCl, 5 mM DTT
PH: 8
Temperature: 30
uniprot:O43252 [sulfate adenylyltransferase(Enzyme) wildtype PAPS synthetase isoform 1]

Chromate + ATP -> Adenylylchromate + Diphosphate		 (( (Vmax * A) ) / (KmA + A)) buffer: 50 mM Tris-HCl, 100 mM NaCl
PH: 8
Temperature: 30
uniprot:O43252 [sulfate adenylyltransferase(Enzyme) wildtype PAPS synthetase isoform 1]

ATP + Chromate -> Adenylylchromate + Diphosphate		 (( (Vmax * B) ) / (KmB + B)) buffer: 50 mM Tris-HCl, 100 mM NaCl
PH: 8
Temperature: 30
uniprot:O43252 [sulfate adenylyltransferase(Enzyme) wildtype PAPS synthetase isoform 1]

ATP + Chromate -> Adenylylchromate + Diphosphate		 (( (Vmax * A) ) / (KmA + A)) buffer: 50 mM Tris-HCl, 100 mM NaCl
PH: 8
Temperature: 30
uniprot:O43252 [sulfate adenylyltransferase(Enzyme) wildtype PAPS synthetase isoform 1]

Chromate + ATP -> Adenylylchromate + Diphosphate		 (( (Vmax * B) ) / (KmB + B)) buffer: 50 mM Tris-HCl, 100 mM NaCl
PH: 8
Temperature: 30
uniprot:O43252 [Chlorate]

Sulfate + ATP -> Diphosphate + Adenylylsulfate		 (( (( (Vmax * A) ) * B) ) / (( (( (( (( (Kia * KmB) ) + (KmA * B)) ) + (KmB * A)) ) + (A * B)) ) + (( (( (Kia * KmB) ) * Q) ) / Kiq))) buffer: 50 mM Tris-HCl, 100 mM NaCl, 5 mM DTT
PH: 8
Temperature: 30
uniprot:O43252 [sulfate adenylyltransferase(Enzyme) wildtype PAPS synthetase isoform 1]

Sulfate + ATP -> Diphosphate + Adenylylsulfate		 (( (Vmax * B) ) / (KmB + B)) buffer: 50 mM Tris-HCl, 100 mM NaCl, 5 mM DTT
PH: 8
Temperature: 30

Pathways

pathway id name
BioCyc:META_PWY-6932 selenate reduction
BioCyc:META_SULFMETII-PWY assimilatory sulfate reduction II
BioCyc:META_SULFATE-CYS-PWY superpathway of sulfate assimilation and cysteine biosynthesis
BioCyc:META_PWY-5345 superpathway of L-methionine biosynthesis (by sulfhydrylation)
BioCyc:META_PWY-5278 sulfite oxidation III
BioCyc:ECO_PWY-5340 sulfate activation for sulfonation
BioCyc:YEAST_PWY-5340 sulfate activation for sulfonation
BioCyc:ARA_PWY-6932 selenate reduction
BioCyc:ARA_PWY-5340 sulfate activation for sulfonation
BioCyc:VCHO_SO4ASSIM-PWY sulfate reduction I (assimilatory)
BioCyc:MTBH37RV_PWY-5345 superpathway of L-methionine biosynthesis (by sulfhydrylation)
BioCyc:MTBH37RV_SULFATE-CYS-PWY superpathway of sulfate assimilation and cysteine biosynthesis
BioCyc:MTBH37RV_PWY-6932 selenate reduction
BioCyc:MTBCDC1551_PWY-6932 selenate reduction
BioCyc:CAULO_SULFATE-CYS-PWY superpathway of sulfate assimilation and cysteine biosynthesis
BioCyc:AGRO_PWY-5345 superpathway of L-methionine biosynthesis (by sulfhydrylation)
BioCyc:AGRO_PWY-5340 sulfate activation for sulfonation
BioCyc:MOUSE_PWY-5340 sulfate activation for sulfonation
BioCyc:BSUB_PWY-5340 sulfate activation for sulfonation
BioCyc:BSUB_SO4ASSIM-PWY assimilatory sulfate reduction I
BioCyc:SYNEL_SO4ASSIM-PWY sulfate reduction I (assimilatory)
BioCyc:ECOL316407_SO4ASSIM-PWY sulfate reduction I (assimilatory)
BioCyc:ECOL316407_SULFATE-CYS-PWY superpathway of sulfate assimilation and cysteine biosynthesis
BioCyc:BTHE_SULFATE-CYS-PWY superpathway of sulfate assimilation and cysteine biosynthesis
BioCyc:BTHE_PWY-5345 superpathway of methionine biosynthesis (by sulfhydrylation)
BioCyc:META_PWY-5340 sulfate activation for sulfonation
BioCyc:META_PWY-821 superpathway of sulfur amino acid biosynthesis (Saccharomyces cerevisiae)
BioCyc:ECO_SO4ASSIM-PWY assimilatory sulfate reduction I
BioCyc:YEAST_SO4ASSIM-PWY sulfate reduction I (assimilatory)
BioCyc:ARA_SULFMETII-PWY sulfate reduction II (assimilatory)
BioCyc:MTBH37RV_PWY-5340 sulfate activation for sulfonation
BioCyc:MTBCDC1551_SULFATE-CYS-PWY superpathway of sulfate assimilation and cysteine biosynthesis
BioCyc:ECOL199310_SO4ASSIM-PWY sulfate reduction I (assimilatory)
BioCyc:ANTHRA_PWY-5340 sulfate activation for sulfonation
BioCyc:ANTHRA_PWY-6932 selenate reduction
BioCyc:MOUSE_PWY3DJ-35888 sulfur metabolism
BioCyc:SYNEL_SULFATE-CYS-PWY superpathway of sulfate assimilation and cysteine biosynthesis
BioCyc:BTHE_PWY-5340 sulfate activation for sulfonation
BioCyc:BTHE_SO4ASSIM-PWY sulfate reduction I (assimilatory)
BioCyc:META_DISSULFRED-PWY dissimilatory sulfate reduction I (to hydrogen sufide))
BioCyc:META_PWY-6395 superpathway of seleno-compound metabolism
BioCyc:META_SO4ASSIM-PWY assimilatory sulfate reduction I
BioCyc:ECO_SULFATE-CYS-PWY superpathway of sulfate assimilation and cysteine biosynthesis
BioCyc:YEAST_PWY-821 superpathway of sulfur amino acid biosynthesis (Saccharomyces cerevisiae)
BioCyc:VCHO_PWY-5340 sulfate activation for sulfonation
BioCyc:VCHO_SULFATE-CYS-PWY superpathway of sulfate assimilation and cysteine biosynthesis
BioCyc:MTBCDC1551_SO4ASSIM-PWY sulfate reduction I (assimilatory)
BioCyc:ECOO157_SULFATE-CYS-PWY superpathway of sulfate assimilation and cysteine biosynthesis
BioCyc:ECOO157_PWY-5340 sulfate activation for sulfonation
BioCyc:CAULO_PWY-5345 superpathway of methionine biosynthesis (by sulfhydrylation)
BioCyc:CAULO_SO4ASSIM-PWY sulfate reduction I (assimilatory)
BioCyc:CAULO_PWY-5340 sulfate activation for sulfonation
BioCyc:ANTHRA_SO4ASSIM-PWY sulfate reduction I (assimilatory)
BioCyc:ANTHRA_PWY-5345 superpathway of L-methionine biosynthesis (by sulfhydrylation)
BioCyc:AGRO_SO4ASSIM-PWY assimilatory sulfate reduction I
BioCyc:BSUB_SULFATE-CYS-PWY superpathway of sulfate assimilation and cysteine biosynthesis
BioCyc:SYNEL_PWY-5340 sulfate activation for sulfonation
BioCyc:BTHE_PWY-6932 selenate reduction
BioCyc:HUMAN_PWY-5340 sulfate activation for sulfonation
BioCyc:META_PWY-6683 assimilatory sulfate reduction III
BioCyc:META_PWY-6676 superpathway of sulfide oxidation (phototrophic sulfur bacteria)
BioCyc:MTBH37RV_SO4ASSIM-PWY assimilatory sulfate reduction I
BioCyc:MTBCDC1551_PWY-5340 sulfate activation for sulfonation
BioCyc:ECOO157_SO4ASSIM-PWY sulfate reduction I (assimilatory)
BioCyc:ECOL199310_PWY-5340 sulfate activation for sulfonation
BioCyc:ECOL199310_SULFATE-CYS-PWY superpathway of sulfate assimilation and cysteine biosynthesis
BioCyc:CAULO_PWY-6683 sulfate reduction III (assimilatory)
BioCyc:ANTHRA_SULFATE-CYS-PWY superpathway of sulfate assimilation and cysteine biosynthesis
BioCyc:AGRO_SULFATE-CYS-PWY superpathway of sulfate assimilation and cysteine biosynthesis
BioCyc:ECOL316407_PWY-5340 sulfate activation for sulfonation
PathBank:SMP0000821 Cysteine Biosynthesis
PathBank:SMP0000939 Sulfur Metabolism
PathBank:SMP0000940 Sulfur Metabolism (Butanesulfonate)
PathBank:SMP0000941 Sulfur Metabolism (Propanesulfonate)
PathBank:SMP0000942 Sulfur Metabolism (Ethanesulfonate)
PathBank:SMP0000943 Sulfur Metabolism (Isethionate)
PathBank:SMP0000944 Sulfur Metabolism (Methanesulfonate)
PlantCyc:ARA_PWY-6932 selenate reduction
PlantCyc:PLANT_SULFMETII-PWY assimilatory sulfate reduction II
PlantCyc:ARA_PWY-6395 superpathway of seleno-compound metabolism
PlantCyc:PLANT_PWY-6932 selenate reduction
PlantCyc:ARA_PWY-5340 sulfate activation for sulfonation
PlantCyc:ARA_SULFMETII-PWY assimilatory sulfate reduction II
PlantCyc:PLANT_PWY-5340 sulfate activation for sulfonation
Reactome:R-HSA-1643685 Disease
Reactome:R-HSA-6802957 Oncogenic MAPK signaling
Reactome:R-MTU-870392 Mycobacterium tuberculosis biological processes
Reactome:R-CEL-71387 Carbohydrate metabolism
Reactome:R-MTU-936635 Sulfate assimilation
Reactome:R-HSA-5663202 Diseases of signal transduction by growth factor receptors and second messengers
Reactome:R-HSA-6802952 Signaling by BRAF and RAF1 fusions
Reactome:R-CEL-1430728 Metabolism
Reactome:R-CEL-1630316 Glycosaminoglycan metabolism
Reactome:R-CEL-174362 Transport and metabolism of PAPS
Reactome:R-MTU-936621 Sulfur compound metabolism
PathBank:SMP0121316 Sulfur Metabolism (Butanesulfonate)
PathBank:SMP0121320 Sulfur Metabolism (Methanesulfonate)
PathBank:SMP0000041 Sulfate/Sulfite Metabolism
PathBank:SMP0000532 Sulfite Oxidase Deficiency
PathBank:SMP0121315 Sulfur Metabolism
PathBank:SMP0121319 Sulfur Metabolism (Isethionate)
PathBank:SMP0063480 Monobactam Biosynthesis
PathBank:SMP0063587 Selenoamino Acid Metabolism
PathBank:SMP0121317 Sulfur Metabolism (Propanesulfonate)
PathBank:SMP0121282 Cysteine Biosynthesis
PathBank:SMP0121318 Sulfur Metabolism (Ethanesulfonate)
PathBank:SMP0000029 Selenoamino Acid Metabolism