Sulfur compound metabolism

Mehra S, Kaushal D. Functional Genomics Reveals Extended Roles of the Mycobacterium tuberculosis Stress Response Factor s H. J Bacteriol. 2009 Jun 15;191(12):3965–80. doi: 10.1128/jb.00064-09.; Agren D, Schnell R, Oehlmann W, Singh M, Schneider G. Cysteine synthase (CysM) of Mycobacterium tuberculosis is an O-phosphoserine sulfhydrylase: evidence for an alternative cysteine biosynthesis pathway in mycobacteria. J Biol Chem. 2008 Nov 14;283(46):31567–74. doi: 10.1074/jbc.m804877200. PMID: 18799456.; O’Leary SE, Jurgenson CT, Ealick SE, Begley TP. O-Phospho-l-serine and the Thiocarboxylated Sulfur Carrier Protein CysO-COSH Are Substrates for CysM, a Cysteine Synthase from Mycobacterium tuberculosis. Biochemistry. 2008 Oct 09;47(44):11606–15. doi: 10.1021/bi8013664.; Hatzios SK, Iavarone AT, Bertozzi CR. Rv2131c from Mycobacterium tuberculosis is a CysQ 3'-phosphoadenosine-5'-phosphatase. Biochemistry. 2008 May 27;47(21):5823–31. PMID: 18454554; PMCID: PMC2711008.; Pinto R, Harrison JS, Hsu T, Jacobs WR, Leyh TS. Sulfite reduction in mycobacteria. J Bacteriol. 2007 Sep;189(18):6714–22. PMID: 17644602; PMCID: PMC2045171.; Schnell R, Oehlmann W, Singh M, Schneider G. Structural insights into catalysis and inhibition of O-acetylserine sulfhydrylase from Mycobacterium tuberculosis. Crystal structures of the enzyme alpha-aminoacrylate intermediate and an enzyme-inhibitor complex. J Biol Chem. 2007 Aug 10;282(32):23473–81. doi: 10.1074/jbc.m703518200. PMID: 17567578.; Devayani P. Bhave, Wilson B. Muse III, Kate S. Carroll. Drug Targets in Mycobacterial Sulfur Metabolism. IDDT. 2007 Jun 01;7(2):140–58. doi: 10.2174/187152607781001772.; Haitani Y, Awano N, Yamazaki M, Wada M, Nakamori S, Takagi H. Functional analysis of L-serine O-acetyltransferase from Corynebacterium glutamicum. FEMS Microbiol Lett. 2006 Feb;255(1):156–63. doi: 10.1111/j.1574-6968.2005.00068.x. PMID: 16436075.; Burns KE, Baumgart S, Dorrestein PC, Zhai H, McLafferty FW, Begley TP. Reconstitution of a New Cysteine Biosynthetic Pathway inMycobacteriumtuberculosis. J. Am. Chem. Soc. 2005 Aug 02;127(33):11602–3. doi: 10.1021/ja053476x.; Schnell R, Sandalova T, Hellman U, Lindqvist Y, Schneider G. Siroheme- and [Fe4-S4]-dependent NirA from Mycobacterium tuberculosis Is a Sulfite Reductase with a Covalent Cys-Tyr Bond in the Active Site. Journal of Biological Chemistry. 2005 Jul;280(29):27319–28. doi: 10.1074/jbc.m502560200.; Carroll KS, Gao H, Chen H, Stout CD, Leary JA, Bertozzi CR. A Conserved Mechanism for Sulfonucleotide Reduction. PLoS Biol. 2005 Jul 19;3(8):e250. doi: 10.1371/journal.pbio.0030250.; Wheeler PR, Coldham NG, Keating L, Gordon SV, Wooff EE, Parish T, Hewinson RG. Functional Demonstration of Reverse Transsulfuration in the Mycobacterium tuberculosis Complex Reveals That Methionine Is the Preferred Sulfur Source for Pathogenic Mycobacteria. Journal of Biological Chemistry. 2005 Mar;280(9):8069–78. doi: 10.1074/jbc.m412540200.; Sun M, Andreassi JL, Liu S, Pinto R, Triccas JA, Leyh TS. The Trifunctional Sulfate-activating Complex (SAC) of Mycobacterium tuberculosis. Journal of Biological Chemistry. 2005 Mar;280(9):7861–6. doi: 10.1074/jbc.m409613200.; Pinto R, Tang QX, Britton WJ, Leyh TS, Triccas JA. The Mycobacterium tuberculosis cysD and cysNC genes form a stress-induced operon that encodes a tri-functional sulfate-activating complex. Microbiology (Reading). 2004 Jun;150(Pt 6):1681–6. doi: 10.1099/mic.0.26894-0. PMID: 15184554.; Williams SJ, Senaratne RH, Mougous JD, Riley LW, Bertozzi CR. 5'-Adenosinephosphosulfate Lies at a Metabolic Branch Point in Mycobacteria. Journal of Biological Chemistry. 2002 Sep;277(36):32606–15. doi: 10.1074/jbc.m204613200.; Wooff E, Michell SL, Gordon SV, Chambers MA, Bardarov S, Jacobs WR, Hewinson RG, Wheeler PR. Functional genomics reveals the sole sulphate transporter of the Mycobacterium tuberculosis complex and its relevance to the acquisition of sulphur in vivo. Molecular Microbiology. 2002 Feb;43(3):653–63. doi: 10.1046/j.1365-2958.2002.02771.x.

Metabolites

2-Oxobutanoate

Formula: C4H6O3 (102.0316926)

CAS ID: 600-18-0

Acetate

Formula: C2H4O2 (60.0211284)

CAS ID: 64-19-7

Acetyl-CoA

Formula: C23H38N7O17P3S (809.1257688000001)

CAS ID: 72-89-9

ADP

Formula: C10H15N5O10P2 (427.02941500000003)

CAS ID: 58-64-0

Ammonia

Formula: NH3 (17.0265478)

CAS ID: 7664-41-7

AMP

Formula: C10H14N5O7P (347.06308240000004)

CAS ID: 61-19-8

ATP

Formula: C10H16N5O13P3 (506.9957476)

CAS ID: 56-65-5

CoA

Formula: C21H36N7O16P3S (767.1152046000001)

CAS ID: 85-61-0

Cystathionine

Formula: C7H14N2O4S (222.0674244)

CAS ID: 535-34-2

Diphosphate

Formula: H4P2O7 (177.9432294)

CAS ID: 2466-09-3

H+

Formula: H (1.0078246)

CAS ID: 12408-02-5

H2O

Formula: H2O (18.0105642)

CAS ID: 7732-18-5

Magnesium cation

Formula: Mg (23.98505)

CAS ID: 22537-22-0

O-Acetyl-L-serine

Formula: C5H9NO4 (147.0531554)

CAS ID: 5147-00-2

O-Phospho-L-serine

Formula: C3H8NO6P (185.0089238)

CAS ID: 407-41-0

Pyridoxal phosphate

Formula: C8H10NO6P (247.024573)

CAS ID: 54-47-7

Sulfate

Formula: H2SO4 (97.9673812)

CAS ID: 7664-93-9

Sulfite

Formula: H2SO3 (81.97246620000001)

CAS ID: 14265-45-3

Sulfur

Formula: S (31.972072)

CAS ID: 7704-34-9

Zinc cation

Formula: Zn (63.929145)

CAS ID: 23713-49-7

phosphate(3-)

Formula: O4P (94.953423)

CAS ID: 14265-44-2



Enzyme

EC Number name full name note
2.7.7.-
1.8.4.10 adenylyl-sulfate reductase (thioredoxin) AMP,sulfite:thioredoxin-disulfide oxidoreductase (adenosine-5'-phosphosulfate-forming)
1.8.7.1 assimilatory sulfite reductase (ferredoxin) hydrogen-sulfide:ferredoxin oxidoreductase
2.3.1.30 serine O-acetyltransferase acetyl-CoA:L-serine O-acetyltransferase
2.5.1.113 [CysO sulfur-carrier protein]-thiocarboxylate-dependent cysteine synthase O-phospho-L-serine:thiocarboxylated [CysO sulfur-carrier protein] 2-amino-2-carboxyethyltransferase
2.5.1.47 cysteine synthase O3-acetyl-L-serine:hydrogen-sulfide 2-amino-2-carboxyethyltransferase
2.5.1.48 cystathionine gamma-synthase O4-succinyl-L-homoserine:L-cysteine S-(3-amino-3-carboxypropyl)transferase
2.5.1.65 O-phosphoserine sulfhydrylase O-phospho-L-serine:hydrogen-sulfide 2-amino-2-carboxyethyltransferase
2.7.1.25 adenylyl-sulfate kinase ATP:adenylyl-sulfate 3'-phosphotransferase
2.7.7.4 sulfate adenylyltransferase ATP:sulfate adenylyltransferase
2.8.5.1 S-sulfo-L-cysteine synthase (3-phospho-L-serine-dependent) thiosulfate:3-phospho-L-serine thiosulfotransferase
3.1.3.11 fructose-bisphosphatase D-fructose-1,6-bisphosphate 1-phosphohydrolase
3.1.3.25 inositol-phosphate phosphatase myo-inositol-phosphate phosphohydrolase
3.1.3.7 3'(2'),5'-bisphosphate nucleotidase adenosine-3'(2'),5'-bisphosphate 3'(2')-phosphohydrolase
3.13.1.6 [CysO sulfur-carrier protein]-S-L-cysteine hydrolase [CysO sulfur-carrier protein]-S-L-cysteine sulfohydrolase
7.3.2.3 ABC-type sulfate transporter ATP phosphohydrolase (ABC-type, sulfate-importing)


Proteins

Protein ID name full name
P95231 cysE Probable serine acetyltransferase CysE (sat)
P9WGB7 metB Cystathionine gamma-synthase
P9WHS1 mec CysO-cysteine peptidase
P9WIK1 cysD Sulfate adenylyltransferase subunit 2
P9WIK3 cysH Adenosine 5'-phosphosulfate reductase
P9WJ03 sirA Ferredoxin-dependent sulfite reductase SirA
P9WKJ1 cysQ 3'-phosphoadenosine 5'-phosphate phosphatase
P9WMN7 moeB1 Probable molybdenum cofactor biosynthesis protein MoeB1 (MPT-synthase sulfurylase) (molybdopterin synthase sulphurylase)
P9WNM5 cysNC Bifunctional enzyme CysN/CysC
P9WP53 cysM O-phosphoserine sulfhydrylase
P9WP55 cysK1 O-acetylserine sulfhydrylase
P9WQM1 cysA Sulfate/thiosulfate import ATP-binding protein CysA
Q79FV4 cysK2 Possible cysteine synthase a CysK2 (O-acetylserine sulfhydrylase) (O-acetylserine (thiol)-lyase) (CSASE)