EC: 2.7.7.18

nicotinate-nucleotide adenylyltransferase (ATP:nicotinate-ribonucleotide adenylyltransferase)

enzyme lambda metabolic reaction experiment
uniprot:Q96T66 [NAD+]

ATP + Nicotinate_D_ribonucleotide -> Diphosphate + Deamino_NAD+		 (( (( (E * kcat) ) * S) ) / (( (Km * (1 + (I / Ki))) ) + (S * (1 + (( (I / alpha) ) * Ki))))) buffer: 27 mM HEPES-KOH, 10 mM NaF, 1 mM DTT, 20 µM MgCl2
PH: 7.5
Temperature: 30
uniprot:Q96T66 [P1-(Adenosine-5')-P4-(nicotinic-acid-riboside-5')-tetraphosphate]

ATP + Nicotinate_D_ribonucleotide -> Diphosphate + Deamino_NAD+		 (( (( (E * kcat) ) * S) ) / (( (Km * (1 + (I / Ki))) ) + (S * (1 + (( (I / alpha) ) * Ki))))) buffer: 27 mM HEPES-KOH, 10 mM NaF, 1 mM DTT, 20 mM MgCl2
PH: 7.5
Temperature: 30
uniprot:Q9BZQ4 [P1-(Adenosine-5')-P4-(nicotinic-acid-riboside-5')-tetraphosphate]

ATP + Nicotinate_D_ribonucleotide -> Deamino_NAD+ + Diphosphate		 (( (( (E * kcat) ) * S) ) / (( (Km * (1 + (I / Ki))) ) + (S * (1 + (( (I / alpha) ) * Ki))))) buffer: 27 mM HEPES-KOH, 10 mM NaF, 1 mM DTT, 20 mM MgCl2
PH: 7.5
Temperature: 30
uniprot:Q96T66 [P1-(Adenosine-5')-P4-(nicotinic-acid-riboside-5')-tetraphosphate]

Nicotinate_D_ribonucleotide + ATP -> Diphosphate + Deamino_NAD+		 (( (( (E * kcat) ) * S) ) / (( (Km * (1 + (I / Ki))) ) + (S * (1 + (( (I / alpha) ) * Ki))))) buffer: 27 mM HEPES-KOH, 10 mM NaF, 1 mM DTT, 20 mM MgCl2
PH: 7.5
Temperature: 30
uniprot:Q9HAN9 [P1-(Adenosine-5')-P4-(nicotinic-acid-riboside-5')-tetraphosphate]

Nicotinate_D_ribonucleotide + ATP -> Diphosphate + Deamino_NAD+		 (( (( (E * kcat) ) * S) ) / (( (Km * (1 + (I / Ki))) ) + (S * (1 + (( (I / alpha) ) * Ki))))) buffer: 27 mM HEPES-KOH, 10 mM NaF, 1 mM DTT, 20 mM MgCl2
PH: 7.5
Temperature: 30
uniprot:Q9HAN9 [P1-(Adenosine-5')-P4-(nicotinic-acid-riboside-5')-tetraphosphate]

ATP + Nicotinate_D_ribonucleotide -> Diphosphate + Deamino_NAD+		 (( (( (E * kcat) ) * S) ) / (( (Km * (1 + (I / Ki))) ) + (S * (1 + (( (I / alpha) ) * Ki))))) buffer: 27 mM HEPES-KOH, 10 mM NaF, 1 mM DTT, 20 mM MgCl2
PH: 7.5
Temperature: 30
uniprot:Q9BZQ4 [P1-(Adenosine-5')-P4-(nicotinic-acid-riboside-5')-tetraphosphate]

ATP + Nicotinate_D_ribonucleotide -> Deamino_NAD+ + Diphosphate		 (( (( (E * kcat) ) * S) ) / (( (Km * (1 + (I / Ki))) ) + (S * (1 + (( (I / alpha) ) * Ki))))) buffer: 27 mM HEPES-KOH, 10 mM NaF, 1 mM DTT, 20 mM MgCl2
PH: 7.5
Temperature: 30
uniprot:Q96T66 [NAD+]

Nicotinate_D_ribonucleotide + ATP -> Deamino_NAD+ + Diphosphate		 (( (( (E * kcat) ) * S) ) / (( (Km * (1 + (I / Ki))) ) + (S * (1 + (( (I / alpha) ) * Ki))))) buffer: 27 mM HEPES-KOH, 10 mM NaF, 1 mM DTT, 20 µM MgCl2
PH: 7.5
Temperature: 30
uniprot:Q96T66 [nicotinate-nucleotide adenylyltransferase(Enzyme) wildtype His-tagged NMNAT3]

ATP + Nicotinate_D_ribonucleotide -> Diphosphate + Deamino_NAD+		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 27 mM HEPES-KOH, 10 mM NaF, 1 mM DTT, 20 mM MgCl2
PH: 7.5
Temperature: 30
uniprot:Q9BZQ4 [nicotinate-nucleotide adenylyltransferase(Enzyme) wildtype His-tagged NMNAT2]

Nicotinate_D_ribonucleotide + ATP -> Deamino_NAD+ + Diphosphate		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 27 mM HEPES-KOH, 10 mM NaF, 1 mM DTT, 20 mM MgCl2
PH: 7.5
Temperature: 30
uniprot:Q9HAN9 [nicotinate-nucleotide adenylyltransferase(Enzyme) wildtype His-tagged NMNAT1]

Nicotinate_D_ribonucleotide + ATP -> Diphosphate + Deamino_NAD+		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 27 mM HEPES-KOH, 10 mM NaF, 1 mM DTT, 20 mM MgCl2
PH: 7.5
Temperature: 30
uniprot:Q96T66 [nicotinate-nucleotide adenylyltransferase(Enzyme) wildtype His-tagged NMNAT3]

Reduced_nicotinamide_nucleotide + ATP -> NADH + Diphosphate + H+		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 27 mM HEPES-KOH, 10 mM NaF, 1 mM DTT, 20 mM MgCl2
PH: 7.5
Temperature: 30
uniprot:Q9BZQ4 [nicotinate-nucleotide adenylyltransferase(Enzyme) wildtype His-tagged NMNAT2]

Reduced_nicotinamide_nucleotide + ATP -> NADH + H+ + Diphosphate		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 27 mM HEPES-KOH, 10 mM NaF, 1 mM DTT, 20 mM MgCl2
PH: 7.5
Temperature: 30
uniprot:Q9HAN9 [nicotinate-nucleotide adenylyltransferase(Enzyme) wildtype His-tagged NMNAT1]

ATP + Reduced_nicotinamide_nucleotide -> NADH + Diphosphate + H+		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 27 mM HEPES-KOH, 10 mM NaF, 1 mM DTT, 20 mM MgCl2
PH: 7.5
Temperature: 30

Pathways

pathway id name
BioCyc:HUMAN_NAD-BIOSYNTHESIS-III NAD salvage
BioCyc:HUMAN_PWY66-401 superpathway of tryptophan utilization
BioCyc:META_PWY0-781 aspartate superpathway
BioCyc:META_PWY-7761 NAD salvage pathway II (PNC IV cycle)
BioCyc:META_PWY-3502 superpathway of NAD biosynthesis in eukaryotes
BioCyc:META_PWY3O-4107 NAD salvage pathway V (PNC V cycle)
BioCyc:YEAST_PWY3O-4107 NAD salvage pathway
BioCyc:MTBH37RV_PYRIDNUCSYN-PWY NAD de novo biosynthesis I (from aspartate)
BioCyc:HPY_PYRIDNUCSYN-PWY NAD biosynthesis I (from aspartate)
BioCyc:BSUB_PWY3O-4106 NAD salvage pathway IV (from nicotinamide riboside)
BioCyc:ECOL316407_PYRIDNUCSYN-PWY NAD biosynthesis I (from aspartate)
BioCyc:ECOL316407_PWY0-781 aspartate superpathway
BioCyc:BTHE_PWY-5653 NAD biosynthesis from 2-amino-3-carboxymuconate semialdehyde
BioCyc:CORYNE_PWY0-781 aspartate superpathway
BioCyc:CORYNE_PWY-5653 NAD biosynthesis from 2-amino-3-carboxymuconate semialdehyde
BioCyc:GCF_000013425_PYRIDNUCSAL-PWY NAD salvage pathway I
BioCyc:META_PYRIDNUCSAL-PWY NAD salvage pathway I (PNC VI cycle)
BioCyc:ECO_PYRIDNUCSYN-PWY NAD de novo biosynthesis I (from aspartate)
BioCyc:ECO_PWY-7761 NAD salvage pathway II (PNC IV cycle)
BioCyc:YEAST_PWY-5653 NAD biosynthesis from 2-amino-3-carboxymuconate semialdehyde
BioCyc:MTBH37RV_PYRIDNUCSAL-PWY NAD salvage pathway I (PNC VI cycle)
BioCyc:MTBCDC1551_PYRIDNUCSAL-PWY NAD salvage pathway I
BioCyc:MOUSE_NADSYN-PWY NAD biosynthesis II (from tryptophan)
BioCyc:GCF_000013425_PYRIDNUCSYN-PWY NAD biosynthesis I (from aspartate)
BioCyc:META_NADSYN-PWY NAD de novo biosynthesis II (from tryptophan)
BioCyc:META_PYRIDNUCSYN-PWY NAD de novo biosynthesis I (from aspartate)
BioCyc:ECO_PYRIDNUCSAL-PWY NAD salvage pathway I (PNC VI cycle)
BioCyc:YEAST_NADSYN-PWY NAD de novo biosynthesis
BioCyc:MTBCDC1551_PYRIDNUCSYN-PWY NAD biosynthesis I (from aspartate)
BioCyc:MOUSE_PWY3O-4106 NAD salvage pathway III
BioCyc:MOUSE_NAD-BIOSYNTHESIS-III NAD biosynthesis III
BioCyc:BSUB_PYRIDNUCSAL-PWY NAD salvage pathway I (PNC VI cycle)
BioCyc:BTHE_PYRIDNUCSYN-PWY NAD biosynthesis I (from aspartate)
BioCyc:BTHE_PYRIDNUCSAL-PWY NAD salvage pathway I
BioCyc:HUMAN_NADSYN-PWY NAD de novo biosynthesis
BioCyc:HUMAN_PWY-5653 NAD biosynthesis from 2-amino-3-carboxymuconate semialdehyde
BioCyc:META_PWY3O-4106 NAD salvage pathway IV (from nicotinamide riboside)
BioCyc:META_PWY-5653 NAD biosynthesis from 2-amino-3-carboxymuconate semialdehyde
BioCyc:ECO_PWY0-781 aspartate superpathway
BioCyc:YEAST_PWY3O-4106 nicotinamide riboside salvage pathway I
BioCyc:MTBH37RV_PWY-5653 NAD biosynthesis from 2-amino-3-carboxymuconate semialdehyde
BioCyc:MTBCDC1551_PWY-5653 NAD biosynthesis from 2-amino-3-carboxymuconate semialdehyde
BioCyc:MOUSE_PWY-5653 NAD biosynthesis from 2-amino-3-carboxymuconate semialdehyde
BioCyc:MOUSE_NAD-BIOSYNTHESIS-II NAD salvage pathway II
BioCyc:MOUSE_PYRNUCYC-PWY pyridine nucleotide cycling
BioCyc:BSUB_PYRIDNUCSYN-PWY NAD de novo biosynthesis I (from aspartate)
BioCyc:ECOL316407_PYRIDNUCSAL-PWY NAD salvage pathway I
BioCyc:CORYNE_PYRIDNUCSYN-PWY NAD biosynthesis I (from aspartate)
PathBank:SMP0000849 NAD Biosynthesis
PathBank:SMP0000850 NAD Salvage
Reactome:R-BTA-1430728 Metabolism
Reactome:R-BTA-196807 Nicotinate metabolism
Reactome:R-DRE-196849 Metabolism of water-soluble vitamins and cofactors
Reactome:R-DME-196807 Nicotinate metabolism
Reactome:R-HSA-196849 Metabolism of water-soluble vitamins and cofactors
Reactome:R-MMU-1430728 Metabolism
Reactome:R-MMU-196849 Metabolism of water-soluble vitamins and cofactors
Reactome:R-RNO-196849 Metabolism of water-soluble vitamins and cofactors
Reactome:R-SPO-1430728 Metabolism
Reactome:R-BTA-196849 Metabolism of water-soluble vitamins and cofactors
Reactome:R-DRE-196854 Metabolism of vitamins and cofactors
Reactome:R-DME-1430728 Metabolism
Reactome:R-DME-196849 Metabolism of water-soluble vitamins and cofactors
Reactome:R-HSA-196854 Metabolism of vitamins and cofactors
Reactome:R-MMU-196854 Metabolism of vitamins and cofactors
Reactome:R-RNO-196854 Metabolism of vitamins and cofactors
Reactome:R-SPO-196807 Nicotinate metabolism
Reactome:R-DRE-1430728 Metabolism
Reactome:R-DRE-196807 Nicotinate metabolism
Reactome:R-HSA-196807 Nicotinate metabolism
Reactome:R-MMU-196807 Nicotinate metabolism
Reactome:R-RNO-1430728 Metabolism
Reactome:R-RNO-196807 Nicotinate metabolism
Reactome:R-SPO-196854 Metabolism of vitamins and cofactors
Reactome:R-BTA-196854 Metabolism of vitamins and cofactors
Reactome:R-DME-196854 Metabolism of vitamins and cofactors
Reactome:R-HSA-1430728 Metabolism
Reactome:R-SPO-196849 Metabolism of water-soluble vitamins and cofactors
PathBank:SMP0083297 NAD+ Signalling Pathway (Cancer)
PathBank:SMP0084271 NAD+ Signalling and Aging
PathBank:SMP0087334 Nicotinate and Nicotinamide Metabolism
PathBank:SMP0087241 Nicotinate and Nicotinamide Metabolism
PathBank:SMP0121297 NAD Biosynthesis
PathBank:SMP0002338 NAD Metabolism
PathBank:SMP0087456 Nicotinate and Nicotinamide Metabolism
PathBank:SMP0121298 NAD Salvage
PathBank:SMP0000048 Nicotinate and Nicotinamide Metabolism