Asparagine N-linked glycosylation

Lederkremer GZ. Glycoprotein folding, quality control and ER-associated degradation. Current Opinion in Structural Biology. 2009 Oct;19(5):515–23. doi: 10.1016/j.sbi.2009.06.004.; Shental-Bechor D, Levy Y. Folding of glycoproteins: toward understanding the biophysics of the glycosylation code. Current Opinion in Structural Biology. 2009 Oct;19(5):524–33. doi: 10.1016/j.sbi.2009.07.002.; Hossler P, Goh LT, Lee MM, Hu WS. GlycoVis: visualizing glycan distribution in the protein N-glycosylation pathway in mammalian cells. Biotechnol Bioeng. 2006 Dec 05;95(5):946–60. doi: 10.1002/bit.21062. PMID: 16807922.; Apweiler R, Hermjakob H, Sharon N. On the frequency of protein glycosylation, as deduced from analysis of the SWISS-PROT database. Biochim Biophys Acta. 1999 Dec 06;1473(1):4–8. doi: 10.1016/s0304-4165(99)00165-8. PMID: 10580125.

Metabolites

alpha-L-Fucopyranose

Formula: C6H12O5 (164.06847019999998)

CAS ID: 6696-41-9

beta-L-Fucopyranose

Formula: C6H12O5 (164.06847019999998)

CAS ID: 13224-93-6

D-Glucose

Formula: C6H12O6 (180.0633852)

CAS ID: 50-99-7

D-Mannose

Formula: C6H12O6 (180.0633852)

CAS ID: 3458-28-4

H+

Formula: H (1.0078246)

CAS ID: 12408-02-5

H2O

Formula: H2O (18.0105642)

CAS ID: 7732-18-5

Magnesium cation

Formula: Mg (23.98505)

CAS ID: 22537-22-0

Manganese(2+)

Formula: Mn (54.938046)

CAS ID: 7439-96-5

N-Acetylneuraminate

Formula: C11H19NO9 (309.10597640000003)

CAS ID: 131-48-6

N-Acetylneuraminate 9-phosphate

Formula: C11H20NO12P (389.0723090000001)

CAS ID: 37992-17-9

Zinc cation

Formula: Zn (63.929145)

CAS ID: 23713-49-7

Beta-1,4-D-Mannosylchitobiosyldiphosphodolichol

Formula: C47H82N2O22P2 (1088.4834212)

CAS ID:

pyruvate

Formula: C3H3O3 (87.00821880000001)

CAS ID: 57-60-3

cholate

Formula: C24H39O5 (407.2797344)

CAS ID: 298-43-1

NADPH(4-)

Formula: C21H26N7O17P3 (741.0598016)

CAS ID: 53-57-6

ATP(4-)

Formula: C10H12N5O13P3 (502.9644492)

CAS ID:

GTP(4-)

Formula: C10H12N5O14P3 (518.9593642)

CAS ID: 86527-72-2

UDP-alpha-D-glucose(2-)

Formula: C15H22N2O17P2 (564.0393702)

CAS ID:

NADH(2-)

Formula: C21H27N7O14P2 (663.1091182000001)

CAS ID:

NAD(1-)

Formula: C21H26N7O14P2 (662.1012936000001)

CAS ID: 53-84-9

isopentenyl diphosphate(3-)

Formula: C5H9O7P2 (242.9823524)

CAS ID:

UDP(3-)

Formula: C9H11N2O12P2 (400.9787246)

CAS ID: 86527-70-0

ADP(3-)

Formula: C10H12N5O10P2 (424.0059412)

CAS ID:

NADP(3-)

Formula: C21H25N7O17P3 (740.051977)

CAS ID: 53-59-8



Enzyme

EC Number name full name note
1.14.11.-
1.3.1.22 3-oxo-5alpha-steroid 4-dehydrogenase (NADP+) 3-oxo-5alpha-steroid:NADP+ DELTA4-oxidoreductase
1.3.1.94 polyprenal reductase dolichal:NADP+ 2,3-oxidoreductase
2.4.1.101 alpha-1,3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase UDP-N-acetyl-alpha-D-glucosamine:alpha-D-mannosyl-(1->3)-beta-D-mannosyl-glycoprotein 2-beta-N-acetyl-D-glucosaminyltransferase (configuration-inverting)
2.4.1.141 N-acetylglucosaminyldiphosphodolichol N-acetylglucosaminyltransferase UDP-N-acetyl-D-glucosamine:N-acetyl-D-glucosaminyl-diphosphodolichol N-acetyl-D-glucosaminyltransferase
2.4.1.143 alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase UDP-N-acetyl-alpha-D-glucosamine:alpha-D-mannosyl-(1->6)-beta-D-mannosyl-glycoprotein 2-beta-N-acetyl-D-glucosaminyltransferase (configuration-inverting)
2.4.1.144 beta-1,4-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase UDP-N-acetyl-alpha-D-glucosamine:beta-D-mannosyl-glycoprotein 4-beta-N-acetyl-D-glucosaminyltransferase (configuration-inverting)
2.4.1.155 alpha-1,6-mannosyl-glycoprotein 6-beta-N-acetylglucosaminyltransferase UDP-N-acetyl-alpha-D-glucosamine:N-acetyl-beta-D-glucosaminyl-(1->2)-alpha-D-mannosyl-(1->6)-beta-D-mannosyl-glycoprotein 6-beta-N-acetyl-D-glucosaminyltransferase (configuration-inverting)
2.4.1.267 dolichyl-P-Glc:Man9GlcNAc2-PP-dolichol alpha-1,3-glucosyltransferase dolichyl beta-D-glucosyl phosphate: D-Man-alpha-(1->2)-D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-[D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-[D-Man-alpha-(1->2)-D-Man-alpha-(1->6)]-D-Man-alpha-(1->6)]-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol alpha-1,3-mannosyltransferase
2.4.1.274 glucosylceramide beta-1,4-galactosyltransferase UDP-galactose:beta-D-glucosyl-(1<->1)-ceramide beta-1,4-galactosyltransferase
2.4.1.275 neolactotriaosylceramide beta-1,4-galactosyltransferase UDP-alpha-D-galactose:N-acetyl-beta-D-glucosaminyl-(1->3)-beta-D-galactosyl-(1beta4)-beta-D-glucosyl-(1<->1)-ceramide 4-beta-D-galactosyltransferase
2.4.1.38 beta-N-acetylglucosaminylglycopeptide beta-1,4-galactosyltransferase UDP-galactose:N-acetyl-beta-D-glucosaminylglycopeptide 4-beta-galactosyltransferase
2.4.1.68 glycoprotein 6-alpha-L-fucosyltransferase GDP-beta-L-fucose:glycoprotein (L-fucose to asparagine-linked N-acetylglucosamine of N4-{N-acetyl-beta-D-glucosaminyl-(1->2)-alpha-D-mannosyl-(1->3)-[N-acetyl-beta-D-glucosaminyl-(1->2)-alpha-D-mannosyl-(1->6)]-beta-D-mannosyl-(1->4)-N-acetyl-beta-D-glucosaminyl-(1->4)-N-acetyl-beta-D-glucosaminyl}asparagine) 6-alpha-L-fucosyltransferase
2.4.1.90 N-acetyllactosamine synthase UDP-galactose:N-acetyl-D-glucosamine 4-beta-D-galactosyltransferase
2.4.3.1 beta-galactoside alpha-(2,6)-sialyltransferase CMP-N-acetyl-beta-neuraminate:beta-D-galactoside alpha-(2->6)-N-acetylneuraminyltransferase (configuration-inverting)
2.4.3.2 beta-D-galactosyl-(1->3)-N-acetyl-beta-D-galactosaminide alpha-2,3-sialyltransferase CMP-N-acetyl-beta-neuraminate:a beta-D-galactosyl-(1->3)-N-acetyl-beta-D-galactosaminyl-R alpha-(2->3)-N-acetylneuraminyltransferase (configuration-inverting)
2.4.3.4 beta-galactoside alpha-2,3-sialyltransferase CMP-N-acetylneuraminate:beta-D-galactoside alpha-(2->3)-N-acetylneuraminyl-transferase
2.4.3.6 N-acetyllactosaminide alpha-2,3-sialyltransferase CMP-N-acetyl-beta-neuraminate:beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosaminyl-R (2->3)-N-acetyl-alpha-neuraminyltransferase (configuration-inverting)
2.7.1.59 N-acetylglucosamine kinase ATP:N-acetyl-D-glucosamine 6-phosphotransferase
2.7.1.60 N-acylmannosamine kinase ATP:N-acyl-D-mannosamine 6-phosphotransferase
2.7.11.1 non-specific serine/threonine protein kinase ATP:protein phosphotransferase (non-specific)
2.7.11.26 tau-protein kinase ATP:[tau-protein] O-phosphotransferase
2.7.7.43 N-acylneuraminate cytidylyltransferase CTP:N-acylneuraminate cytidylyltransferase
3.1.3.16 protein-serine/threonine phosphatase protein-serine/threonine-phosphate phosphohydrolase
3.1.3.29 N-acylneuraminate-9-phosphatase N-acylneuraminate-9-phosphate phosphohydrolase
3.2.1.114 mannosyl-oligosaccharide 1,3-1,6-alpha-mannosidase (1->3)-(1->6)-mannosyl-oligosaccharide alpha-D-mannohydrolase (configuration-retaining)
3.2.1.130 glycoprotein endo-alpha-1,2-mannosidase glycoprotein glucosylmannohydrolase
3.2.1.18 exo-alpha-sialidase acetylneuraminyl hydrolase
3.2.1.183 UDP-N-acetylglucosamine 2-epimerase (hydrolysing) UDP-N-acetyl-alpha-D-glucosamine hydrolase (2-epimerising)
3.2.1.51 alpha-L-fucosidase alpha-L-fucoside fucohydrolase
3.5.1.25 N-acetylglucosamine-6-phosphate deacetylase N-acetyl-D-glucosamine-6-phosphate amidohydrolase
3.5.1.52 peptide-N4-(N-acetyl-beta-glucosaminyl)asparagine amidase N-linked-glycopeptide-(N-acetyl-beta-D-glucosaminyl)-L-asparagine amidohydrolase
3.6.4.6 vesicle-fusing ATPase ATP phosphohydrolase (vesicle-fusing)
3.6.5.2 small monomeric GTPase GTP phosphohydrolase (cell-regulating)
4.1.1.33 diphosphomevalonate decarboxylase ATP:(R)-5-diphosphomevalonate carboxy-lyase (adding ATP; isopentenyl-diphosphate-forming)
4.1.3.3 N-acetylneuraminate lyase aceneuramate pyruvate-lyase (N-acetyl-D-mannosamine-forming)
5.1.3.8 N-acylglucosamine 2-epimerase N-Acyl-D-glucosamine 2-epimerase
5.3.1.8 mannose-6-phosphate isomerase D-mannose-6-phosphate aldose-ketose-isomerase
5.3.4.1 protein disulfide-isomerase Protein disulfide-isomerase


Proteins

Protein ID name full name