EC: 2.4.1.101

alpha-1,3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase (UDP-N-acetyl-alpha-D-glucosamine:alpha-D-mannosyl-(1->3)-beta-D-mannosyl-glycoprotein 2-beta-N-acetyl-D-glucosaminyltransferase (configuration-inverting))

enzyme lambda metabolic reaction experiment
uniprot:Q9XGM8 [alpha-1,3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase(Enzyme) wildtype GnTI, catalytic domain fused to secretion signal, His-tagged]

UDP_N_acetyl_D_glucosamine + Man_alpha1_6(Man_alpha1_3)Man_beta_1_O_octyl -> GlcNAc_beta(1_2)Man3_octyl + UDP		 (( (Vmax * S) ) / (Km + S)) buffer: 0.1 M 2-(N-morpholino)ethane-sulfonic acid, 0.5% Triton X-100, 0.5 mM phenylmethylsulfonyl fluoride, 0.5 mM dithiothreitol, 2.5 µg/ml leupeptin, 2.5 µg/ml E-64, 5 mM AMP, 20 mM MnCl, 5 µg/ml Swainsonine, 0.1 mM 2-acetoamido-1,2-dideoxynojirimycin
PH: 6.3
Temperature: 37
uniprot:P27115 [alpha-1,3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase(Enzyme) wildtype GnTI, catalytic domain fused to secretion signal ]

UDP_N_acetyl_D_glucosamine + Man_alpha1_6(Man_alpha1_3)Man_beta_1_O_octyl -> GlcNAc_beta(1_2)Man3_octyl + UDP		 (( (Vmax * S) ) / (Km + S)) buffer: 0.1 M 2-(N-morpholino)ethane-sulfonic acid, 0.5% Triton X-100, 0.5 mM phenylmethylsulfonyl fluoride, 0.5 mM dithiothreitol, 2.5 µg/ml leupeptin, 2.5 µg/ml E-64, 5 mM AMP, 20 mM MnCl, 5 µg/ml Swainsonine, 0.1 mM 2-acetoamido-1,2-dideoxynojirimycin
PH: 6.3
Temperature: 37
uniprot:P27115 [alpha-1,3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase(Enzyme) wildtype GnTI, catalytic domain fused to secretion signal ]

UDP_N_acetyl_D_glucosamine + Man_alpha1_6(Man_alpha1_3)Man_alpha1_6(Man_alpha1_3)Man_beta_1_4GlcNAc_beta1_4GlcNAc_peptide -> UDP + GlcNAc_beta(1_2)Man5GlcNAc2_glycopeptide		 (( (Vmax * S) ) / (Km + S)) buffer: 0.1 M 2-(N-morpholino)ethane-sulfonic acid, 0.5% Triton X-100, 0.5 mM phenylmethylsulfonyl fluoride, 0.5 mM dithiothreitol, 2.5 µg/ml leupeptin, 2.5 µg/ml E-64, 5 mM AMP, 20 mM MnCl, 5 µg/ml Swainsonine, 0.1 mM 2-acetoamido-1,2-dideoxynojirimycin
PH: 6.3
Temperature: 37
uniprot:Q9XGM8 [alpha-1,3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase(Enzyme) wildtype GnTI, catalytic domain fused to secretion signal, His-tagged]

UDP_N_acetyl_D_glucosamine + Man_alpha1_6(Man_alpha1_3)Man_alpha1_6(Man_alpha1_3)Man_beta_1_4GlcNAc_beta1_4GlcNAc_peptide -> UDP + GlcNAc_beta(1_2)Man5GlcNAc2_glycopeptide		 (( (Vmax * S) ) / (Km + S)) buffer: 0.1 M 2-(N-morpholino)ethane-sulfonic acid, 0.5% Triton X-100, 0.5 mM phenylmethylsulfonyl fluoride, 0.5 mM dithiothreitol, 2.5 µg/ml leupeptin, 2.5 µg/ml E-64, 5 mM AMP, 20 mM MnCl, 5 µg/ml Swainsonine, 0.1 mM 2-acetoamido-1,2-dideoxynojirimycin
PH: 6.3
Temperature: 37
uniprot:P27115 [alpha-1,3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase(Enzyme) mutant D144N, catalytic domain fused to secretion signal ]

Man_alpha1_6(Man_alpha1_3)Man_alpha1_6(Man_alpha1_3)Man_beta_1_4GlcNAc_beta1_4GlcNAc_peptide + UDP_N_acetyl_D_glucosamine -> UDP + GlcNAc_beta(1_2)Man5GlcNAc2_glycopeptide		 (( (Vmax * S) ) / (Km + S)) buffer: 0.1 M 2-(N-morpholino)ethane-sulfonic acid, 0.5% Triton X-100, 0.5 mM phenylmethylsulfonyl fluoride, 0.5 mM dithiothreitol, 2.5 µg/ml leupeptin, 2.5 µg/ml E-64, 5 mM AMP, 20 mM MnCl, 5 µg/ml Swainsonine, 0.1 mM 2-acetoamido-1,2-dideoxynojirimycin
PH: 6.3
Temperature: 37
uniprot:P27115 [alpha-1,3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase(Enzyme) wildtype GnTI, catalytic domain fused to secretion signal ]

Man_alpha1_6(Man_alpha1_3)Man_alpha1_6(Man_alpha1_3)Man_beta_1_4GlcNAc_beta1_4GlcNAc_peptide + UDP_N_acetyl_D_glucosamine -> GlcNAc_beta(1_2)Man5GlcNAc2_glycopeptide + UDP		 (( (Vmax * S) ) / (Km + S)) buffer: 0.1 M 2-(N-morpholino)ethane-sulfonic acid, 0.5% Triton X-100, 0.5 mM phenylmethylsulfonyl fluoride, 0.5 mM dithiothreitol, 2.5 µg/ml leupeptin, 2.5 µg/ml E-64, 5 mM AMP, 20 mM MnCl, 5 µg/ml Swainsonine, 0.1 mM 2-acetoamido-1,2-dideoxynojirimycin
PH: 6.3
Temperature: 37
uniprot:P27115 [alpha-1,3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase(Enzyme) mutant D144N, catalytic domain fused to secretion signal ]

Man_alpha1_6(Man_alpha1_3)Man_alpha1_6(Man_alpha1_3)Man_beta_1_4GlcNAc_beta1_4GlcNAc_peptide + UDP_N_acetyl_D_glucosamine -> GlcNAc_beta(1_2)Man5GlcNAc2_glycopeptide + UDP		 (( (Vmax * S) ) / (Km + S)) buffer: 0.1 M 2-(N-morpholino)ethane-sulfonic acid, 0.5% Triton X-100, 0.5 mM phenylmethylsulfonyl fluoride, 0.5 mM dithiothreitol, 2.5 µg/ml leupeptin, 2.5 µg/ml E-64, 5 mM AMP, 20 mM MnCl, 5 µg/ml Swainsonine, 0.1 mM 2-acetoamido-1,2-dideoxynojirimycin
PH: 6.3
Temperature: 37
uniprot:Q9XGM8 [alpha-1,3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase(Enzyme) wildtype GnTI, catalytic domain fused to secretion signal, His-tagged]

UDP_N_acetyl_D_glucosamine + Man_alpha1_6(Man_alpha1_3)Man_alpha1_6(Man_alpha1_3)Man_beta_1_4GlcNAc_beta1_4GlcNAc_peptide -> UDP + GlcNAc_beta(1_2)Man5GlcNAc2_glycopeptide		 (( (Vmax * S) ) / (Km + S)) buffer: 0.1 M 2-(N-morpholino)ethane-sulfonic acid, 0.5% Triton X-100, 0.5 mM phenylmethylsulfonyl fluoride, 0.5 mM dithiothreitol, 2.5 µg/ml leupeptin, 2.5 µg/ml E-64, 5 mM AMP, 20 mM MnCl, 5 µg/ml Swainsonine, 0.1 mM 2-acetoamido-1,2-dideoxynojirimycin
PH: 6.3
Temperature: 37

Pathways

pathway id name
BioCyc:META_PWY-7426 complex N-linked glycan biosynthesis (vertebrates)
BioCyc:META_PWY-7920 complex N-linked glycan biosynthesis (plants)
BioCyc:HUMAN_PWY-7426 complex N-linked glycan biosynthesis (vertebrates)
Reactome:R-HSA-9679506 SARS-CoV Infections
Reactome:R-HSA-9694516 SARS-CoV-2 Infection
Reactome:R-HSA-9694635 Translation of Structural Proteins
Reactome:R-HSA-9683686 Maturation of spike protein
Reactome:R-HSA-9678108 SARS-CoV-1 Infection
Reactome:R-HSA-9683701 Translation of Structural Proteins
Reactome:R-HSA-9772573 Late SARS-CoV-2 Infection Events
Reactome:R-HSA-9824446 Viral Infection Pathways
Reactome:R-HSA-9694548 Maturation of spike protein
Reactome:R-CEL-964739 N-glycan trimming and elongation in the cis-Golgi
Reactome:R-HSA-392499 Metabolism of proteins
Reactome:R-HSA-597592 Post-translational protein modification
Reactome:R-HSA-1643685 Disease
Reactome:R-RNO-597592 Post-translational protein modification
Reactome:R-CEL-392499 Metabolism of proteins
Reactome:R-CEL-597592 Post-translational protein modification
Reactome:R-CEL-446203 Asparagine N-linked glycosylation
Reactome:R-CEL-948021 Transport to the Golgi and subsequent modification
Reactome:R-MMU-964739 N-glycan trimming and elongation in the cis-Golgi
Reactome:R-RNO-392499 Metabolism of proteins
Reactome:R-HSA-446203 Asparagine N-linked glycosylation
Reactome:R-HSA-948021 Transport to the Golgi and subsequent modification
Reactome:R-MMU-392499 Metabolism of proteins
Reactome:R-MMU-597592 Post-translational protein modification
Reactome:R-RNO-446203 Asparagine N-linked glycosylation
Reactome:R-RNO-948021 Transport to the Golgi and subsequent modification
Reactome:R-HSA-964739 N-glycan trimming and elongation in the cis-Golgi
Reactome:R-HSA-5663205 Infectious disease
Reactome:R-MMU-446203 Asparagine N-linked glycosylation
Reactome:R-MMU-948021 Transport to the Golgi and subsequent modification
Reactome:R-RNO-964739 N-glycan trimming and elongation in the cis-Golgi
WikiPathways:WP5021 SARS-CoV-2 Infection
WikiPathways:WP5020 SARS-CoV-1 Infection