EC: 4.1.3.3

N-acetylneuraminate lyase (aceneuramate pyruvate-lyase (N-acetyl-D-mannosamine-forming))

enzyme lambda metabolic reaction experiment
uniprot:P0A6L4 [N-acetylneuraminate lyase(Enzyme) wildtype]

N_Acetyl_D_neuraminate -> Pyruvate + N_Acetyl_D_mannosamine		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM potassium phosphate, 5 mM 2-mercaptoethanol, 360 mM NADH, 0.67 U/ml Lactic dehydrogenase
PH: 7.5
Temperature: 25
uniprot:P0A6L4 [N-acetylneuraminate lyase(Enzyme) mutant N5B2]

N_Acetyl_D_neuraminate -> N_Acetyl_D_mannosamine + Pyruvate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM potassium phosphate, 5 mM 2-mercaptoethanol, 360 mM NADH, 0.67 U/ml Lactic dehydrogenase
PH: 7.5
Temperature: 25
uniprot:P0A6L4 [N-acetylneuraminate lyase(Enzyme) wildtype]

N_Acetyl_L_neuraminate -> N_Acetyl_L_mannosamine + Pyruvate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM potassium phosphate, 5 mM 2-mercaptoethanol, 360 mM NADH, 0.67 U/ml Lactic dehydrogenase
PH: 7.5
Temperature: 25
uniprot:P0A6L4 [N-acetylneuraminate lyase(Enzyme) mutant N2C5]

N_Acetyl_L_neuraminate -> Pyruvate + N_Acetyl_L_mannosamine		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM potassium phosphate, 5 mM 2-mercaptoethanol, 360 mM NADH, 0.67 U/ml Lactic dehydrogenase
PH: 7.5
Temperature: 25
uniprot:P0A6L4 [N-acetylneuraminate lyase(Enzyme) mutant N3A4]

N_Acetyl_L_neuraminate -> N_Acetyl_L_mannosamine + Pyruvate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM potassium phosphate, 5 mM 2-mercaptoethanol, 360 mM NADH, 0.67 U/ml Lactic dehydrogenase
PH: 7.5
Temperature: 25
uniprot:P0A6L4 [N-acetylneuraminate lyase(Enzyme) mutant N5B2]

N_Acetyl_L_neuraminate -> Pyruvate + N_Acetyl_L_mannosamine		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM potassium phosphate, 5 mM 2-mercaptoethanol, 360 mM NADH, 0.67 U/ml Lactic dehydrogenase
PH: 7.5
Temperature: 25
uniprot:P0A6L4 [N-acetylneuraminate lyase(Enzyme) mutant N4E6]

N_Acetyl_L_neuraminate -> N_Acetyl_L_mannosamine + Pyruvate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM potassium phosphate, 5 mM 2-mercaptoethanol, 360 mM NADH, 0.67 U/ml Lactic dehydrogenase
PH: 7.5
Temperature: 25
uniprot:P0A6L4 [N-acetylneuraminate lyase(Enzyme) mutant N2C5]

N_Acetyl_D_neuraminate -> Pyruvate + N_Acetyl_D_mannosamine		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM potassium phosphate, 5 mM 2-mercaptoethanol, 360 mM NADH, 0.67 U/ml Lactic dehydrogenase
PH: 7.5
Temperature: 25
uniprot:P0A6L4 [N-acetylneuraminate lyase(Enzyme) mutant N1G8]

N_Acetyl_D_neuraminate -> Pyruvate + N_Acetyl_D_mannosamine		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM potassium phosphate, 5 mM 2-mercaptoethanol, 360 mM NADH, 0.67 U/ml Lactic dehydrogenase
PH: 7.5
Temperature: 25
uniprot:P0A6L4 [N-acetylneuraminate lyase(Enzyme) mutant N3A4]

N_Acetyl_D_neuraminate -> Pyruvate + N_Acetyl_D_mannosamine		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM potassium phosphate, 5 mM 2-mercaptoethanol, 360 mM NADH, 0.67 U/ml Lactic dehydrogenase
PH: 7.5
Temperature: 25
uniprot:P0A6L4 [N-acetylneuraminate lyase(Enzyme) mutant N4E6]

N_Acetyl_D_neuraminate -> Pyruvate + N_Acetyl_D_mannosamine		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM potassium phosphate, 5 mM 2-mercaptoethanol, 360 mM NADH, 0.67 U/ml Lactic dehydrogenase
PH: 7.5
Temperature: 25

Pathways

pathway id name
BioCyc:META_PWY0-1324 N-acetylneuraminate and N-acetylmannosamine degradation I
BioCyc:META_P441-PWY superpathway of N-acetylneuraminate degradation
BioCyc:ECOO157_P441-PWY superpathway of N-acetylneuraminate degradation
BioCyc:ECOL199310_PWY0-1324 N-acetylneuraminate and N-acetylmannosamine degradation
BioCyc:GCF_000013425_PWY0-1324 N-acetylneuraminate and N-acetylmannosamine degradation I
BioCyc:ECO_PWY0-1324 N-acetylneuraminate and N-acetylmannosamine degradation I
BioCyc:MOUSE_GLCMANNANAUT-PWY superpathway of N-acetylglucosamine, N-acetylmannosamine and N-acetylneuraminate degradation
BioCyc:ECOL316407_GLCMANNANAUT-PWY superpathway of N-acetylglucosamine, N-acetylmannosamine and N-acetylneuraminate degradation
BioCyc:META_GLCMANNANAUT-PWY superpathway of N-acetylglucosamine, N-acetylmannosamine and N-acetylneuraminate degradation
BioCyc:ECO_GLCMANNANAUT-PWY superpathway of N-acetylglucosamine, N-acetylmannosamine and N-acetylneuraminate degradation
BioCyc:ECOO157_GLCMANNANAUT-PWY superpathway of N-acetylglucosamine, N-acetylmannosamine and N-acetylneuraminate degradation
BioCyc:ECOL199310_GLCMANNANAUT-PWY superpathway of N-acetylglucosamine, N-acetylmannosamine and N-acetylneuraminate degradation
BioCyc:ECOL316407_PWY0-1324 N-acetylneuraminate and N-acetylmannosamine degradation
BioCyc:GCF_000013425_GLCMANNANAUT-PWY superpathway of N-acetylglucosamine, N-acetylmannosamine and N-acetylneuraminate degradation
BioCyc:ECOO157_PWY0-1324 N-acetylneuraminate and N-acetylmannosamine degradation
BioCyc:MOUSE_PWY0-1324 N-acetylneuraminate and N-acetylmannosamine degradation
PathBank:SMP0002044 N-Acetylneuraminate, N-Acetylmannosamine, and N-Acetylglucosamine Degradation
Reactome:R-BTA-597592 Post-translational protein modification
Reactome:R-BTA-4085001 Sialic acid metabolism
Reactome:R-DRE-392499 Metabolism of proteins
Reactome:R-DRE-446219 Synthesis of substrates in N-glycan biosythesis
Reactome:R-HSA-392499 Metabolism of proteins
Reactome:R-HSA-597592 Post-translational protein modification
Reactome:R-HSA-4085001 Sialic acid metabolism
Reactome:R-MMU-446219 Synthesis of substrates in N-glycan biosythesis
Reactome:R-RNO-597592 Post-translational protein modification
Reactome:R-RNO-4085001 Sialic acid metabolism
Reactome:R-SSC-446193 Biosynthesis of the N-glycan precursor (dolichol lipid-linked oligosaccharide, LLO) and transfer to a nascent protein
Reactome:R-XTR-446203 Asparagine N-linked glycosylation
Reactome:R-BTA-446219 Synthesis of substrates in N-glycan biosythesis
Reactome:R-DRE-446193 Biosynthesis of the N-glycan precursor (dolichol lipid-linked oligosaccharide, LLO) and transfer to a nascent protein
Reactome:R-HSA-446219 Synthesis of substrates in N-glycan biosythesis
Reactome:R-MMU-446193 Biosynthesis of the N-glycan precursor (dolichol lipid-linked oligosaccharide, LLO) and transfer to a nascent protein
Reactome:R-RNO-392499 Metabolism of proteins
Reactome:R-RNO-446219 Synthesis of substrates in N-glycan biosythesis
Reactome:R-SSC-392499 Metabolism of proteins
Reactome:R-SSC-446203 Asparagine N-linked glycosylation
Reactome:R-XTR-597592 Post-translational protein modification
Reactome:R-XTR-4085001 Sialic acid metabolism
Reactome:R-BTA-392499 Metabolism of proteins
Reactome:R-BTA-446203 Asparagine N-linked glycosylation
Reactome:R-DRE-4085001 Sialic acid metabolism
Reactome:R-HSA-446203 Asparagine N-linked glycosylation
Reactome:R-MMU-392499 Metabolism of proteins
Reactome:R-MMU-597592 Post-translational protein modification
Reactome:R-MMU-4085001 Sialic acid metabolism
Reactome:R-RNO-446203 Asparagine N-linked glycosylation
Reactome:R-SSC-446219 Synthesis of substrates in N-glycan biosythesis
Reactome:R-XTR-392499 Metabolism of proteins
Reactome:R-XTR-446193 Biosynthesis of the N-glycan precursor (dolichol lipid-linked oligosaccharide, LLO) and transfer to a nascent protein
Reactome:R-BTA-446193 Biosynthesis of the N-glycan precursor (dolichol lipid-linked oligosaccharide, LLO) and transfer to a nascent protein
Reactome:R-DRE-597592 Post-translational protein modification
Reactome:R-DRE-446203 Asparagine N-linked glycosylation
Reactome:R-HSA-446193 Biosynthesis of the N-glycan precursor (dolichol lipid-linked oligosaccharide, LLO) and transfer to a nascent protein
Reactome:R-MMU-446203 Asparagine N-linked glycosylation
Reactome:R-RNO-446193 Biosynthesis of the N-glycan precursor (dolichol lipid-linked oligosaccharide, LLO) and transfer to a nascent protein
Reactome:R-SSC-597592 Post-translational protein modification
Reactome:R-SSC-4085001 Sialic acid metabolism
Reactome:R-XTR-446219 Synthesis of substrates in N-glycan biosythesis
PathBank:SMP0120818 G(M2)-Gangliosidosis: Variant B, Tay-Sachs Disease
PathBank:SMP0120524 Salla Disease/Infantile Sialic Acid Storage Disease
PathBank:SMP0000240 Salla Disease/Infantile Sialic Acid Storage Disease
PathBank:SMP0120737 Sialuria or French Type Sialuria
PathBank:SMP0120765 Tay-Sachs Disease
PathBank:SMP0000045 Amino Sugar Metabolism
PathBank:SMP0000216 Sialuria or French Type Sialuria
PathBank:SMP0087281 Amino Sugar Metabolism
PathBank:SMP0120744 Salla Disease/Infantile Sialic Acid Storage Disease
PathBank:SMP0120517 Sialuria or French Type Sialuria
PathBank:SMP0120545 Tay-Sachs Disease
PathBank:SMP0000390 Tay-Sachs Disease
PathBank:SMP0087175 Amino Sugar Metabolism
PathBank:SMP0120599 G(M2)-Gangliosidosis: Variant B, Tay-Sachs Disease
PathBank:SMP0000534 G(M2)-Gangliosidosis: Variant B, Tay-Sachs Disease
PathBank:SMP0063580 Amino Sugar Metabolism