EC: 2.7.1.59

N-acetylglucosamine kinase (ATP:N-acetyl-D-glucosamine 6-phosphotransferase)

enzyme lambda metabolic reaction experiment
- [N-acetylglucosamine kinase(Enzyme) wildtype]

ATP + N_Acetyl_D_glucosamine -> ADP + N_Acetyl_D_glucosamine_6_phosphate		 (( (V * A) ) / (Km + A)) buffer: 0.02 M MgCl2, 0.002 M EDTA, 0.02 M NaF
PH: 7.4
Temperature: 30
uniprot:A0KYQ6 [N-acetylglucosamine kinase(Enzyme) wildtype His6-tagged]

N_Acetyl_D_glucosamine + ATP -> ADP + N_Acetyl_D_glucosamine_6_phosphate		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM Tris, 20 mM MgCl2, 2 mM phosphoenolpyruvate, 0.3 mM NADH, 20 U/ml pyruvate kinase, 20 U/ml lactate dehydrogenase
PH: 7.5
Temperature: 30
uniprot:P75959 [N-acetylglucosamine kinase(Enzyme) wildtype]

D_Glucose + ATP -> ADP + D_Glucose_6_phosphate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 0.3-0.5 M Tris-HCl
PH: 7.6
Temperature: 25
uniprot:P75959 [N-acetylglucosamine kinase(Enzyme) wildtype]

ATP + D_Glucose -> ADP + D_Glucose_6_phosphate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 0.3-0.5 M Tris-HCl
PH: 7.6
Temperature: 25
uniprot:P75959 [N-acetylglucosamine kinase(Enzyme) wildtype NagK]

Glucose + ATP -> ADP + Glucose_6_phosphate		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM Tris-HCl, 10 mM MgCl2, 1 mM Phosphoenolpyruvate, 0.2 mM NADH, 8 U/ml Lactate dehydrogenase, 8 U/ml Pyruvate kinase
PH: 7.5
Temperature: 37
uniprot:P75959 [N-acetylglucosamine kinase(Enzyme) wildtype NagK]

N_Acetyl_D_glucosamine + ATP -> N_Acetyl_D_glucosamine_6_phosphate + ADP		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM Tris-HCl, 10 mM MgCl2, 1 mM Phosphoenolpyruvate, 0.2 mM NADH, 8 U/ml Lactate dehydrogenase, 8 U/ml Pyruvate kinase
PH: 7.5
Temperature: 37
uniprot:P75959 [N-acetylglucosamine kinase(Enzyme) wildtype NagK]

ATP + N_Acetyl_D_glucosamine -> N_Acetyl_D_glucosamine_6_phosphate + ADP		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM Tris-HCl, 10 mM MgCl2, 1 mM Phosphoenolpyruvate, 0.2 mM NADH, 8 U/ml Lactate dehydrogenase, 8 U/ml Pyruvate kinase
PH: 7.5
Temperature: 37
uniprot:Q9QZ08 [N-acetylglucosamine kinase(Enzyme) wildtype His-tagged]

N_Acetyl_D_glucosamine + ATP -> ADP + N_Acetyl_D_glucosamine_6_phosphate		 (( (Vmax * S1) ) / (Km + S1)) buffer: 60 mM Tris-HCl, 20 mM MgCl2, 10 mM Phosphoenolpyruvate, 2.5 U Pyruvate kinase
PH: 7.5
Temperature: 37
uniprot:Q9QZ08 [N-acetylglucosamine kinase(Enzyme) wildtype His-tagged]

ATP + N_Acetyl_D_glucosamine -> ADP + N_Acetyl_D_glucosamine_6_phosphate		 (( (Vmax * S1) ) / (Km + S1)) buffer: 60 mM Tris-HCl, 20 mM MgCl2, 10 mM Phosphoenolpyruvate, 2.5 U Pyruvate kinase
PH: 7.5
Temperature: 37

Pathways

pathway id name
BioCyc:HUMAN_PWY-6517 N-acetylglucosamine degradation II
BioCyc:META_PWY-7077 N-acetyl-D-galactosamine degradation
BioCyc:YEAST_PWY-6981 chitin biosynthesis
BioCyc:YEAST_ANAGLYCOLYSIS-PWY glycolysis III (from glucose)
BioCyc:MOUSE_PWY-6517 N-acetylglucosamine degradation II
BioCyc:META_PWY-6517 N-acetylglucosamine degradation II
BioCyc:YEAST_PWY3O-3827 glucose-6-phosphate biosynthesis
BioCyc:YEAST_PWY0-1182 trehalose degradation II (cytosolic)
BioCyc:YEAST_PWY-621 sucrose degradation III
BioCyc:ECOL199310_PWY-6517 N-acetylglucosamine degradation II
WikiPathways:WP3641 SK UDPglcnac
PathBank:SMP0000905 Amino Sugar and Nucleotide Sugar Metabolism I
PathBank:SMP0000906 Amino Sugar and Nucleotide Sugar Metabolism II
PathBank:SMP0000914 Amino Sugar and Nucleotide Sugar Metabolism III
Reactome:R-HSA-392499 Metabolism of proteins
Reactome:R-HSA-597592 Post-translational protein modification
Reactome:R-MMU-446219 Synthesis of substrates in N-glycan biosythesis
Reactome:R-RNO-597592 Post-translational protein modification
Reactome:R-HSA-446219 Synthesis of substrates in N-glycan biosythesis
Reactome:R-MMU-446193 Biosynthesis of the N-glycan precursor (dolichol lipid-linked oligosaccharide, LLO) and transfer to a nascent protein
Reactome:R-RNO-392499 Metabolism of proteins
Reactome:R-RNO-446219 Synthesis of substrates in N-glycan biosythesis
Reactome:R-HSA-446203 Asparagine N-linked glycosylation
Reactome:R-HSA-446210 Synthesis of UDP-N-acetyl-glucosamine
Reactome:R-MMU-392499 Metabolism of proteins
Reactome:R-MMU-597592 Post-translational protein modification
Reactome:R-RNO-446203 Asparagine N-linked glycosylation
Reactome:R-RNO-446210 Synthesis of UDP-N-acetyl-glucosamine
Reactome:R-HSA-446193 Biosynthesis of the N-glycan precursor (dolichol lipid-linked oligosaccharide, LLO) and transfer to a nascent protein
Reactome:R-MMU-446203 Asparagine N-linked glycosylation
Reactome:R-MMU-446210 Synthesis of UDP-N-acetyl-glucosamine
Reactome:R-RNO-446193 Biosynthesis of the N-glycan precursor (dolichol lipid-linked oligosaccharide, LLO) and transfer to a nascent protein
PathBank:SMP0120666 4-Hydroxybutyric Aciduria/Succinic Semialdehyde Dehydrogenase Deficiency
PathBank:SMP0120714 Homocarnosinosis
PathBank:SMP0120818 G(M2)-Gangliosidosis: Variant B, Tay-Sachs Disease
PathBank:SMP0120850 Succinic Semialdehyde Dehydrogenase Deficiency
PathBank:SMP0120496 Hyperinsulinism-Hyperammonemia Syndrome
PathBank:SMP0120524 Salla Disease/Infantile Sialic Acid Storage Disease
PathBank:SMP0000339 Hyperinsulinism-Hyperammonemia Syndrome
PathBank:SMP0000240 Salla Disease/Infantile Sialic Acid Storage Disease
PathBank:SMP0002331 Amino Sugar and Nucleotide Sugar Metabolism
PathBank:SMP0087169 Glutamate Metabolism
PathBank:SMP0087278 Glutamate Metabolism
PathBank:SMP0120737 Sialuria or French Type Sialuria
PathBank:SMP0120765 Tay-Sachs Disease
PathBank:SMP0000045 Amino Sugar Metabolism
PathBank:SMP0000385 Homocarnosinosis
PathBank:SMP0000216 Sialuria or French Type Sialuria
PathBank:SMP0000136 2-Hydroxyglutric Aciduria (D and L Form)
PathBank:SMP0000567 Succinic Semialdehyde Dehydrogenase Deficiency
PathBank:SMP0087281 Amino Sugar Metabolism
PathBank:SMP0120716 Hyperinsulinism-Hyperammonemia Syndrome
PathBank:SMP0120744 Salla Disease/Infantile Sialic Acid Storage Disease
PathBank:SMP0120445 4-Hydroxybutyric Aciduria/Succinic Semialdehyde Dehydrogenase Deficiency
PathBank:SMP0120517 Sialuria or French Type Sialuria
PathBank:SMP0120545 Tay-Sachs Disease
PathBank:SMP0000390 Tay-Sachs Disease
PathBank:SMP0087175 Amino Sugar Metabolism
PathBank:SMP0063590 Glutamate Metabolism
PathBank:SMP0120659 2-Hydroxyglutric Aciduria (D and L Form)
PathBank:SMP0120438 2-Hydroxyglutric Aciduria (D and L Form)
PathBank:SMP0120494 Homocarnosinosis
PathBank:SMP0120599 G(M2)-Gangliosidosis: Variant B, Tay-Sachs Disease
PathBank:SMP0120631 Succinic Semialdehyde Dehydrogenase Deficiency
PathBank:SMP0000072 Glutamate Metabolism
PathBank:SMP0000243 4-Hydroxybutyric Aciduria/Succinic Semialdehyde Dehydrogenase Deficiency
PathBank:SMP0000534 G(M2)-Gangliosidosis: Variant B, Tay-Sachs Disease
PathBank:SMP0002380 Starch and Sucrose Metabolism
PathBank:SMP0063580 Amino Sugar Metabolism