EC: 3.2.1.51

alpha-L-fucosidase (alpha-L-fucoside fucohydrolase)

enzyme lambda metabolic reaction experiment
uniprot:Q9BTY2 [alpha-L-fucosidase(Enzyme) wildtype from infantile fucosidosis patient]

4_Methylumbelliferyl_alpha_L_fucopyranoside + H2O -> L_Fucose + 4_Methylumbelliferone		 (( (Vmax * S) ) / (Km + S)) buffer: 500 mM Citrate
PH: 6
Temperature: 37
uniprot:Q9BTY2 [alpha-L-fucosidase(Enzyme) wildtype from infantile fucosidosis patient]

H2O + 4_Methylumbelliferyl_alpha_L_fucopyranoside -> 4_Methylumbelliferone + L_Fucose		 (( (Vmax * S) ) / (Km + S)) buffer: 500 mM Citrate
PH: 6
Temperature: 37
uniprot:Q9BTY2 [alpha-L-fucosidase(Enzyme) wildtype]

4_Methylumbelliferyl_alpha_L_fucopyranoside + H2O -> 4_Methylumbelliferone + L_Fucose		 (( (Vmax * S) ) / (Km + S)) buffer: 500 mM Citrate
PH: 6
Temperature: 37
uniprot:P04066 [alpha-L-fucosidase(Enzyme) wildtype isoenzyme IV (pI 5.75)]

p_Nitrophenyl_alpha_L_fucopyranoside + H2O -> p_Nitrophenol + L_Fucose		 (( (Vmax * S) ) / (Km + S)) buffer: 100 mM citrate-sodium citrate
PH: 5
Temperature: 37
uniprot:P04066 [alpha-L-fucosidase(Enzyme) wildtype isoenzyme VI (pI 5.43)]

p_Nitrophenyl_alpha_L_fucopyranoside + H2O -> p_Nitrophenol + L_Fucose		 (( (Vmax * S) ) / (Km + S)) buffer: 100 mM citrate-sodium citrate
PH: 5
Temperature: 37
uniprot:P04066 [alpha-L-fucosidase(Enzyme) wildtype isoenzyme II (pI 6.25)]

p_Nitrophenyl_alpha_L_fucopyranoside + H2O -> L_Fucose + p_Nitrophenol		 (( (Vmax * S) ) / (Km + S)) buffer: 100 mM citrate-sodium citrate
PH: 5
Temperature: 37
uniprot:P04066 [alpha-L-fucosidase(Enzyme) wildtype isoenzyme III (pI 5.98)]

p_Nitrophenyl_alpha_L_fucopyranoside + H2O -> p_Nitrophenol + L_Fucose		 (( (Vmax * S) ) / (Km + S)) buffer: 100 mM citrate-sodium citrate
PH: 5
Temperature: 37
uniprot:P04066 [alpha-L-fucosidase(Enzyme) wildtype isoenzyme I (pI 6.57)]

H2O + p_Nitrophenyl_alpha_L_fucopyranoside -> L_Fucose + p_Nitrophenol		 (( (Vmax * S) ) / (Km + S)) buffer: 100 mM citrate-sodium citrate
PH: 5
Temperature: 37
uniprot:P04066 [alpha-L-fucosidase(Enzyme) wildtype isoenzyme V (pI 5.58)]

H2O + p_Nitrophenyl_alpha_L_fucopyranoside -> L_Fucose + p_Nitrophenol		 (( (Vmax * S) ) / (Km + S)) buffer: 100 mM citrate-sodium citrate
PH: 5
Temperature: 37
uniprot:P04066 [alpha-L-fucosidase(Enzyme) wildtype isoenzyme VII (pI 4.97)]

p_Nitrophenyl_alpha_L_fucopyranoside + H2O -> L_Fucose + p_Nitrophenol		 (( (Vmax * S) ) / (Km + S)) buffer: 100 mM citrate-sodium citrate
PH: 5
Temperature: 37
uniprot:P04066 [alpha-L-fucosidase(Enzyme) wildtype]

H2O + 4_Methylumbelliferyl_alpha_L_fucopyranoside -> L_Fucose + 4_Methylumbelliferone		 (( (Vmax * S) ) / (Km + S)) buffer: 100 mM citrate
PH: 5
Temperature: 37
uniprot:P04066 [alpha-L-fucosidase(Enzyme) wildtype]

H2O + 4_Methylumbelliferyl_alpha_L_fucopyranoside -> L_Fucose + 4_Methylumbelliferone		 (( (Vmax * S) ) / (Km + S)) buffer: 100 mM citrate
PH: 5
Temperature: 37
uniprot:P04066 [alpha-L-fucosidase(Enzyme) wildtype]

H2O + p_Nitrophenyl_alpha_L_fucopyranoside -> p_Nitrophenol + L_Fucose		 (( (Vmax * S) ) / (Km + S)) buffer: 100 mM citrate-sodium citrate
PH: 5
Temperature: 37
uniprot:P04066 [alpha-L-fucosidase(Enzyme) wildtype]

4_Methylumbelliferyl_alpha_L_fucopyranoside + H2O -> L_Fucose + 4_Methylumbelliferone		 (( (Vmax * A) ) / (Km + A)) buffer: 0.25 M Na2HPO4/NaH2PO4
PH: 5.7
Temperature: 37

Pathways

pathway id name
Reactome:R-BTA-597592 Post-translational protein modification
Reactome:R-BTA-975576 N-glycan antennae elongation in the medial/trans-Golgi
Reactome:R-DME-597592 Post-translational protein modification
Reactome:R-HSA-392499 Metabolism of proteins
Reactome:R-HSA-597592 Post-translational protein modification
Reactome:R-HSA-975576 N-glycan antennae elongation in the medial/trans-Golgi
Reactome:R-RNO-597592 Post-translational protein modification
Reactome:R-RNO-975576 N-glycan antennae elongation in the medial/trans-Golgi
Reactome:R-CEL-392499 Metabolism of proteins
Reactome:R-CEL-597592 Post-translational protein modification
Reactome:R-CEL-446203 Asparagine N-linked glycosylation
Reactome:R-CEL-948021 Transport to the Golgi and subsequent modification
Reactome:R-CEL-975578 Reactions specific to the complex N-glycan synthesis pathway
Reactome:R-DME-975578 Reactions specific to the complex N-glycan synthesis pathway
Reactome:R-MMU-975578 Reactions specific to the complex N-glycan synthesis pathway
Reactome:R-RNO-392499 Metabolism of proteins
Reactome:R-BTA-392499 Metabolism of proteins
Reactome:R-BTA-446203 Asparagine N-linked glycosylation
Reactome:R-BTA-948021 Transport to the Golgi and subsequent modification
Reactome:R-DME-392499 Metabolism of proteins
Reactome:R-DME-975576 N-glycan antennae elongation in the medial/trans-Golgi
Reactome:R-HSA-446203 Asparagine N-linked glycosylation
Reactome:R-HSA-948021 Transport to the Golgi and subsequent modification
Reactome:R-MMU-392499 Metabolism of proteins
Reactome:R-MMU-597592 Post-translational protein modification
Reactome:R-MMU-975576 N-glycan antennae elongation in the medial/trans-Golgi
Reactome:R-RNO-446203 Asparagine N-linked glycosylation
Reactome:R-RNO-948021 Transport to the Golgi and subsequent modification
Reactome:R-BTA-975578 Reactions specific to the complex N-glycan synthesis pathway
Reactome:R-CEL-975576 N-glycan antennae elongation in the medial/trans-Golgi
Reactome:R-DME-446203 Asparagine N-linked glycosylation
Reactome:R-DME-948021 Transport to the Golgi and subsequent modification
Reactome:R-HSA-975578 Reactions specific to the complex N-glycan synthesis pathway
Reactome:R-MMU-446203 Asparagine N-linked glycosylation
Reactome:R-MMU-948021 Transport to the Golgi and subsequent modification
Reactome:R-RNO-975578 Reactions specific to the complex N-glycan synthesis pathway