EC: 2.8.2.2

alcohol sulfotransferase (3'-phosphoadenylyl-sulfate:alcohol sulfotransferase)

enzyme lambda metabolic reaction experiment
uniprot:Q29YR5 [alcohol sulfotransferase(Enzyme) wildtype SULT2B1a isoform]

3'_Phosphoadenylyl_sulfate + 3beta_Hydroxyandrost_5_en_17_one -> Adenosine_3',5'_bisphosphate + 3beta_Hydroxyandrost_5_en_17_one_3_sulfate		 (( (Vmax * A) ) / (Km + A)) buffer: 0.1 mM Tris-HCl, 5 mM MgCl2, 0.2 mM 2-hydroxypropyl-beta-cyclodextrin, 4 % ethanol (v/v)
PH: 7.5
Temperature: 37
uniprot:Q29YR5 [alcohol sulfotransferase(Enzyme) wildtype SULT2B1a isoform]

3'_Phosphoadenylyl_sulfate + Pregnenolone -> Pregnenolone_sulfate + Adenosine_3',5'_bisphosphate		 (( (Vmax * A) ) / (Km + A)) buffer: 0.1 mM Tris-HCl, 5 mM MgCl2, 0.2 mM 2-hydroxypropyl-beta-cyclodextrin, 4 % ethanol (v/v)
PH: 7.5
Temperature: 37
uniprot:Q29YR5 [alcohol sulfotransferase(Enzyme) wildtype SULT2B1b isoform]

Cholesterol + 3'_Phosphoadenylyl_sulfate -> Adenosine_3',5'_bisphosphate + Cholesterol_sulfate		 (( (Vmax * A) ) / (Km + A)) buffer: 0.1 mM Tris-HCl, 5 mM MgCl2, 0.2 mM 2-hydroxypropyl-beta-cyclodextrin, 4 % ethanol (v/v)
PH: 7.5
Temperature: 37
uniprot:O35400 [alcohol sulfotransferase(Enzyme) wildtype]

3'_Phosphoadenylyl_sulfate + 3beta_Hydroxyandrost_5_en_17_one -> 3beta_Hydroxyandrost_5_en_17_one_3_sulfate + Adenosine_3',5'_bisphosphate		 (( (Vmax * A) ) / (Km + A)) buffer: 200 mM Tris-acetate
PH: 7.9
Temperature: 37
uniprot:O35400 [alcohol sulfotransferase(Enzyme) wildtype]

Estradiol_17beta + 3'_Phosphoadenylyl_sulfate -> Estradiol_17beta_3_sulfate + Adenosine_3',5'_bisphosphate		 (( (Vmax * A) ) / (Km + A)) buffer: 200 mM Tris-acetate
PH: 7.9
Temperature: 37
uniprot:O35400 [alcohol sulfotransferase(Enzyme) wildtype]

Diethylstilbestrol + 3'_Phosphoadenylyl_sulfate -> Diethylstilbestrol_sulfate + Adenosine_3',5'_bisphosphate		 (( (Vmax * A) ) / (Km + A)) buffer: 200 mM Tris-acetate
PH: 7.9
Temperature: 37
uniprot:Q06520 [alcohol sulfotransferase(Enzyme) mutant P76T]

3'_Phosphoadenylyl_sulfate + 3beta_Hydroxyandrost_5_en_17_one -> 3beta_Hydroxyandrost_5_en_17_one_3_sulfate + Adenosine_3',5'_bisphosphate		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM HEPES, 1 mM DTT
PH: 7.4
Temperature: 37
uniprot:Q06520 [alcohol sulfotransferase(Enzyme) wildtype]

3beta_Hydroxyandrost_5_en_17_one + 3'_Phosphoadenylyl_sulfate -> Adenosine_3',5'_bisphosphate + 3beta_Hydroxyandrost_5_en_17_one_3_sulfate		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM HEPES, 1 mM DTT
PH: 7.4
Temperature: 37
uniprot:Q06520 [alcohol sulfotransferase(Enzyme) mutant E147K]

3'_Phosphoadenylyl_sulfate + 3beta_Hydroxyandrost_5_en_17_one -> 3beta_Hydroxyandrost_5_en_17_one_3_sulfate + Adenosine_3',5'_bisphosphate		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM HEPES, 1 mM DTT
PH: 7.4
Temperature: 37
uniprot:Q06520 [alcohol sulfotransferase(Enzyme) mutant E148K]

3beta_Hydroxyandrost_5_en_17_one + 3'_Phosphoadenylyl_sulfate -> Adenosine_3',5'_bisphosphate + 3beta_Hydroxyandrost_5_en_17_one_3_sulfate		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM HEPES, 1 mM DTT
PH: 7.4
Temperature: 37
uniprot:Q06520 [alcohol sulfotransferase(Enzyme) mutant P76T]

3beta_Hydroxyandrost_5_en_17_one + 3'_Phosphoadenylyl_sulfate -> Adenosine_3',5'_bisphosphate + 3beta_Hydroxyandrost_5_en_17_one_3_sulfate		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM HEPES, 1 mM DTT
PH: 7.4
Temperature: 37
uniprot:Q06520 [alcohol sulfotransferase(Enzyme) wildtype]

3'_Phosphoadenylyl_sulfate + 3beta_Hydroxyandrost_5_en_17_one -> 3beta_Hydroxyandrost_5_en_17_one_3_sulfate + Adenosine_3',5'_bisphosphate		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM HEPES, 1 mM DTT
PH: 7.4
Temperature: 37
uniprot:Q06520 [alcohol sulfotransferase(Enzyme) mutant E148K]

3'_Phosphoadenylyl_sulfate + 3beta_Hydroxyandrost_5_en_17_one -> 3beta_Hydroxyandrost_5_en_17_one_3_sulfate + Adenosine_3',5'_bisphosphate		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM HEPES, 1 mM DTT
PH: 7.4
Temperature: 37
uniprot:Q06520 [alcohol sulfotransferase(Enzyme) mutant L246P]

3'_Phosphoadenylyl_sulfate + 3beta_Hydroxyandrost_5_en_17_one -> Adenosine_3',5'_bisphosphate + 3beta_Hydroxyandrost_5_en_17_one_3_sulfate		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM HEPES, 1 mM DTT
PH: 7.4
Temperature: 37
uniprot:Q06520 [alcohol sulfotransferase(Enzyme) mutant E147K]

3beta_Hydroxyandrost_5_en_17_one + 3'_Phosphoadenylyl_sulfate -> 3beta_Hydroxyandrost_5_en_17_one_3_sulfate + Adenosine_3',5'_bisphosphate		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM HEPES, 1 mM DTT
PH: 7.4
Temperature: 37
uniprot:Q06520 [alcohol sulfotransferase(Enzyme) mutant F258L]

3'_Phosphoadenylyl_sulfate + 3beta_Hydroxyandrost_5_en_17_one -> 3beta_Hydroxyandrost_5_en_17_one_3_sulfate + Adenosine_3',5'_bisphosphate		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM HEPES, 1 mM DTT
PH: 7.4
Temperature: 37
uniprot:Q06520 [alcohol sulfotransferase(Enzyme) mutant Q262E]

3beta_Hydroxyandrost_5_en_17_one + 3'_Phosphoadenylyl_sulfate -> 3beta_Hydroxyandrost_5_en_17_one_3_sulfate + Adenosine_3',5'_bisphosphate		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM HEPES, 1 mM DTT
PH: 7.4
Temperature: 37
uniprot:Q06520 [alcohol sulfotransferase(Enzyme) mutant L246P]

3'_Phosphoadenylyl_sulfate + 3beta_Hydroxyandrost_5_en_17_one -> 3beta_Hydroxyandrost_5_en_17_one_3_sulfate + Adenosine_3',5'_bisphosphate		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM HEPES, 1 mM DTT
PH: 7.4
Temperature: 37
uniprot:Q06520 [alcohol sulfotransferase(Enzyme) mutant F258L]

3beta_Hydroxyandrost_5_en_17_one + 3'_Phosphoadenylyl_sulfate -> 3beta_Hydroxyandrost_5_en_17_one_3_sulfate + Adenosine_3',5'_bisphosphate		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM HEPES, 1 mM DTT
PH: 7.4
Temperature: 37
uniprot:Q06520 [alcohol sulfotransferase(Enzyme) mutant Q262E]

3'_Phosphoadenylyl_sulfate + 3beta_Hydroxyandrost_5_en_17_one -> 3beta_Hydroxyandrost_5_en_17_one_3_sulfate + Adenosine_3',5'_bisphosphate		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM HEPES, 1 mM DTT
PH: 7.4
Temperature: 37

Pathways

pathway id name
Reactome:R-MMU-9753281 APAP ADME
Reactome:R-HSA-9753281 APAP ADME
Reactome:R-MMU-9748784 Drug ADME
Reactome:R-HSA-9748784 Drug ADME
Reactome:R-HSA-400206 Regulation of lipid metabolism by PPARalpha
Reactome:R-HSA-211859 Biological oxidations
Reactome:R-MMU-1430728 Metabolism
Reactome:R-MMU-211859 Biological oxidations
Reactome:R-HSA-556833 Metabolism of lipids
Reactome:R-HSA-156580 Phase II - Conjugation of compounds
Reactome:R-MMU-156580 Phase II - Conjugation of compounds
Reactome:R-RNO-211859 Biological oxidations
Reactome:R-HSA-1989781 PPARA activates gene expression
Reactome:R-RNO-1430728 Metabolism
Reactome:R-RNO-156584 Cytosolic sulfonation of small molecules
Reactome:R-HSA-1430728 Metabolism
Reactome:R-HSA-156584 Cytosolic sulfonation of small molecules
Reactome:R-MMU-156584 Cytosolic sulfonation of small molecules
Reactome:R-RNO-156580 Phase II - Conjugation of compounds
PathBank:SMP0120597 Sulfite Oxidase Deficiency
PathBank:SMP0120629 Aromatase Deficiency
PathBank:SMP0000041 Sulfate/Sulfite Metabolism
PathBank:SMP0000068 Androgen and Estrogen Metabolism
PathBank:SMP0000532 Sulfite Oxidase Deficiency
PathBank:SMP0063690 Sulfate/Sulfite Metabolism
PathBank:SMP0000356 17-beta Hydroxysteroid Dehydrogenase III Deficiency
PathBank:SMP0000640 Acetaminophen Metabolism Pathway
PathBank:SMP0120437 17-beta Hydroxysteroid Dehydrogenase III Deficiency
PathBank:SMP0000565 Aromatase Deficiency
PathBank:SMP0063594 Androgen and Estrogen Metabolism