EC: 4.1.1.48

indole-3-glycerol-phosphate synthase (1-(2-carboxyphenylamino)-1-deoxy-D-ribulose-5-phosphate carboxy-lyase [cyclizing; 1-C-(indol-3-yl)glycerol-3-phosphate-forming])

enzyme lambda metabolic reaction experiment
uniprot:Q8U088 [indole-3-glycerol-phosphate synthase(Enzyme) wildtype]

1_(2_Carboxyphenylamino)_1_deoxy_D_ribulose_5_phosphate -> H2O + CO2 + (3_Indolyl)_glycerol_phosphate		 (( (Vmax * S) ) / (Km + S)) buffer: 0.2 M sodium phosphate
PH: 5.5
Temperature: 55
uniprot:Q56319 [indole-3-glycerol-phosphate synthase(Enzyme) wildtype]

1_(2_Carboxyphenylamino)_1_deoxy_D_ribulose_5_phosphate -> CO2 + (3_Indolyl)_glycerol_phosphate + H2O		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 0.05 M EPPS, 4 mM EDTA, 1 mM DTT
PH: 7.5
Temperature: 80
uniprot:Q56319 [indole-3-glycerol-phosphate synthase(Enzyme) wildtype]

1_(2_Carboxyphenylamino)_1_deoxy_D_ribulose_5_phosphate -> CO2 + H2O + (3_Indolyl)_glycerol_phosphate		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 0.05 M EPPS, 4 mM EDTA, 1 mM DTT
PH: 7.5
Temperature: 60
uniprot:Q56319 [indole-3-glycerol-phosphate synthase(Enzyme) wildtype]

1_(2_Carboxyphenylamino)_1_deoxy_D_ribulose_5_phosphate -> H2O + CO2 + (3_Indolyl)_glycerol_phosphate		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 0.05 M EPPS, 4 mM EDTA, 1 mM DTT
PH: 7.5
Temperature: 45
uniprot:Q56319 [indole-3-glycerol-phosphate synthase(Enzyme) wildtype]

1_(2_Carboxyphenylamino)_1_deoxy_D_ribulose_5_phosphate -> H2O + (3_Indolyl)_glycerol_phosphate + CO2		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 0.05 M EPPS, 4 mM EDTA, 1 mM DTT
PH: 7.5
Temperature: 25
uniprot:Q56319 [indole-3-glycerol-phosphate synthase(Enzyme) mutant R241A]

1_(2_Carboxyphenylamino)_1_deoxy_D_ribulose_5_phosphate -> (3_Indolyl)_glycerol_phosphate + CO2 + H2O		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 0.05 M EPPS, 4 mM EDTA, 1 mM DTT, 1 mM phosphate
PH: 7.5
Temperature: 60
uniprot:P9WFX7 [indole-3-glycerol-phosphate synthase(Enzyme) wildtype]

1_(2_Carboxyphenylamino)_1_deoxy_D_ribulose_5_phosphate -> (3_Indolyl)_glycerol_phosphate + CO2 + H2O		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 100 mM PIPES
PH: 7.5
Temperature: 25
uniprot:Q56319 [indole-3-glycerol-phosphate synthase(Enzyme) wildtype]

1_(2_Carboxyphenylamino)_1_deoxy_D_ribulose_5_phosphate -> (3_Indolyl)_glycerol_phosphate + H2O + CO2		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 0.05 M EPPS, 4 mM EDTA, 1 mM DTT, 1 mM phosphate
PH: 7.5
Temperature: 60
uniprot:Q56319 [indole-3-glycerol-phosphate synthase(Enzyme) mutant D184A]

1_(2_Carboxyphenylamino)_1_deoxy_D_ribulose_5_phosphate -> H2O + CO2 + (3_Indolyl)_glycerol_phosphate		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 0.05 M EPPS, 4 mM EDTA, 1 mM DTT, 1 mM phosphate
PH: 7.5
Temperature: 60
uniprot:Q06121 [indole-3-glycerol-phosphate synthase(Enzyme) wildtype]

1_(2_Carboxyphenylamino)_1_deoxy_D_ribulose_5_phosphate -> CO2 + (3_Indolyl)_glycerol_phosphate + H2O		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 50 mM HEPPS, 4 mM EDTA
PH: 7.5
Temperature: 50
uniprot:Q06121 [indole-3-glycerol-phosphate synthase(Enzyme) wildtype]

1_(2_Carboxyphenylamino)_1_deoxy_D_ribulose_5_phosphate -> H2O + (3_Indolyl)_glycerol_phosphate + CO2		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 50 mM HEPPS, 4 mM EDTA
PH: 7.5
Temperature: 75
uniprot:P00909 [indole-3-glycerol-phosphate synthase(Enzyme) wildtype truncated TrpC (aa 1-259)]

1_(2_Carboxyphenylamino)_1_deoxy_D_ribulose_5_phosphate -> H2O + CO2 + (3_Indolyl)_glycerol_phosphate		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 50 mM HEPPS, 4 mM EDTA
PH: 7.5
Temperature: 37
uniprot:P00909 [indole-3-glycerol-phosphate synthase(Enzyme) wildtype truncated TrpC (aa 1-259)]

1_(2_Carboxyphenylamino)_1_deoxy_D_ribulose_5_phosphate -> CO2 + H2O + (3_Indolyl)_glycerol_phosphate		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 50 mM HEPPS, 4 mM EDTA
PH: 7.5
Temperature: 25
uniprot:Q06121 [indole-3-glycerol-phosphate synthase(Enzyme) wildtype]

1_(2_Carboxyphenylamino)_1_deoxy_D_ribulose_5_phosphate -> (3_Indolyl)_glycerol_phosphate + CO2 + H2O		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 50 mM HEPPS, 4 mM EDTA
PH: 7.5
Temperature: 25
uniprot:Q06121 [indole-3-glycerol-phosphate synthase(Enzyme) wildtype]

1_(2_Carboxyphenylamino)_1_deoxy_D_ribulose_5_phosphate -> H2O + CO2 + (3_Indolyl)_glycerol_phosphate		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 50 mM HEPPS, 4 mM EDTA
PH: 7.5
Temperature: 37
uniprot:P20577 [indole-3-glycerol-phosphate synthase(Enzyme) wildtype C-terminal His6-tag]

1_(2_Carboxyphenylamino)_1_deoxy_D_ribulose_5_phosphate -> (3_Indolyl)_glycerol_phosphate + CO2 + H2O		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 200 mM NaCl, 0.5 mM MgCl2, 0.5 mM β-mercaptoethanol
PH: 8
Temperature: 37
uniprot:Q06121 [indole-3-glycerol-phosphate synthase(Enzyme) wildtype C-terminal His6-tag]

1_(2_Carboxyphenylamino)_1_deoxy_D_ribulose_5_phosphate -> CO2 + (3_Indolyl)_glycerol_phosphate + H2O		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 50 mM HEPES, 4 mM EDTA
PH: 7.5
Temperature: 60
uniprot:P00899 [indole-3-glycerol-phosphate synthase(Enzyme) wildtype]

1_(2_Carboxyphenylamino)_1_deoxy_D_ribulose_5_phosphate -> (3_Indolyl)_glycerol_phosphate + CO2 + H2O		 (( (Vmax * S) ) / (Km + S)) buffer: 0.1 M Tris-HCl, 0.1 mg/ml BSA
PH: 7.8
Temperature: 37
uniprot:Q06121 [indole-3-glycerol-phosphate synthase(Enzyme) mutant M237T C-terminal His6-tag]

1_(2_Carboxyphenylamino)_1_deoxy_D_ribulose_5_phosphate -> (3_Indolyl)_glycerol_phosphate + H2O + CO2		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 50 mM HEPES, 4 mM EDTA
PH: 7.5
Temperature: 25
uniprot:Q06121 [indole-3-glycerol-phosphate synthase(Enzyme) mutant F246S C-terminal His6-tag]

1_(2_Carboxyphenylamino)_1_deoxy_D_ribulose_5_phosphate -> (3_Indolyl)_glycerol_phosphate + H2O + CO2		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 50 mM HEPES, 4 mM EDTA
PH: 7.5
Temperature: 25
uniprot:Q06121 [indole-3-glycerol-phosphate synthase(Enzyme) wildtype C-terminal His6-tag]

1_(2_Carboxyphenylamino)_1_deoxy_D_ribulose_5_phosphate -> H2O + (3_Indolyl)_glycerol_phosphate + CO2		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 50 mM HEPES, 4 mM EDTA
PH: 7.5
Temperature: 25
uniprot:Q06121 [indole-3-glycerol-phosphate synthase(Enzyme) mutant G212E C-terminal His6-tag]

1_(2_Carboxyphenylamino)_1_deoxy_D_ribulose_5_phosphate -> H2O + (3_Indolyl)_glycerol_phosphate + CO2		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 50 mM HEPES, 4 mM EDTA
PH: 7.5
Temperature: 25
uniprot:Q06121 [indole-3-glycerol-phosphate synthase(Enzyme) mutant L236Q C-terminal His6-tag]

1_(2_Carboxyphenylamino)_1_deoxy_D_ribulose_5_phosphate -> H2O + (3_Indolyl)_glycerol_phosphate + CO2		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 50 mM HEPES, 4 mM EDTA
PH: 7.5
Temperature: 25
uniprot:P00909 [indole-3-glycerol-phosphate synthase(Enzyme) wildtype IGPS:PRAI]

1_(2_Carboxyphenylamino)_1_deoxy_D_ribulose_5_phosphate -> H2O + CO2 + (3_Indolyl)_glycerol_phosphate		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 0.05 M Tris-HCl, 4 mM K2-MgEDTA, 2 mM DTE
PH: 7.5
Temperature: 25
uniprot:P00909 [indole-3-glycerol-phosphate synthase(Enzyme) mutant IGPS[1-259]]

1_(2_Carboxyphenylamino)_1_deoxy_D_ribulose_5_phosphate -> (3_Indolyl)_glycerol_phosphate + H2O + CO2		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 0.05 M Tris-HCl, 4 mM K2-MgEDTA, 2 mM DTE
PH: 7.5
Temperature: 25
uniprot:P00909 [indole-3-glycerol-phosphate synthase(Enzyme) wildtype IGPS:PRAI]

1_(2_Carboxyphenylamino)_1_deoxy_D_ribulose_5_phosphate -> CO2 + (3_Indolyl)_glycerol_phosphate + H2O		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 0.1 M Tris-HCl, 5 mM EDTA, 2 mM DTE
PH: 7.8
Temperature: 25
uniprot:P00909 [indole-3-glycerol-phosphate synthase(Enzyme) mutant IGPS[1:252FAG]]

1_(2_Carboxyphenylamino)_1_deoxy_D_ribulose_5_phosphate -> (3_Indolyl)_glycerol_phosphate + CO2 + H2O		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 0.1 M Tris-HCl, 5 mM EDTA, 2 mM DTE
PH: 7.8
Temperature: 25

Pathways

pathway id name
BioCyc:META_ALL-CHORISMATE-PWY superpathway of chorismate metabolism
BioCyc:ECO_COMPLETE-ARO-PWY superpathway of aromatic amino acid biosynthesis
BioCyc:YEAST_TRPSYN-PWY tryptophan biosynthesis
BioCyc:MTBH37RV_PWY-6629 superpathway of L-tryptophan biosynthesis
BioCyc:CAULO_COMPLETE-ARO-PWY superpathway of phenylalanine, tyrosine, and tryptophan biosynthesis
BioCyc:BSUB_TRPSYN-PWY L-tryptophan biosynthesis
BioCyc:ECOL316407_TRPSYN-PWY tryptophan biosynthesis
BioCyc:ECOL316407_ALL-CHORISMATE-PWY superpathway of chorismate metabolism
BioCyc:BTHE_TRPSYN-PWY tryptophan biosynthesis
BioCyc:CORYNE_PWY-6629 superpathway of L-tryptophan biosynthesis
BioCyc:GCF_000013425_TRPSYN-PWY L-tryptophan biosynthesis
BioCyc:VCHO_ALL-CHORISMATE-PWY superpathway of chorismate metabolism
BioCyc:VCHO_PWY-6629 superpathway of L-tryptophan biosynthesis
BioCyc:HPY_TRPSYN-PWY L-tryptophan biosynthesis
BioCyc:CAULO_TRPSYN-PWY tryptophan biosynthesis
BioCyc:AGRO_TRPSYN-PWY L-tryptophan biosynthesis
BioCyc:AGRO_COMPLETE-ARO-PWY superpathway of aromatic amino acid biosynthesis
BioCyc:ECOL316407_COMPLETE-ARO-PWY superpathway of phenylalanine, tyrosine, and tryptophan biosynthesis
BioCyc:CORYNE_TRPSYN-PWY L-tryptophan biosynthesis
BioCyc:META_COMPLETE-ARO-PWY superpathway of aromatic amino acid biosynthesis
BioCyc:YEAST_COMPLETE-ARO-YEAST-PWY superpathway of phenylalanine, tyrosine and tryptophan biosynthesis
BioCyc:VCHO_TRPSYN-PWY L-tryptophan biosynthesis
BioCyc:MTBH37RV_TRPSYN-PWY L-tryptophan biosynthesis
BioCyc:CAULONA1000_TRPSYN-PWY tryptophan biosynthesis
BioCyc:CORYNE_COMPLETE-ARO-PWY superpathway of aromatic amino acid biosynthesis
BioCyc:META_TRPSYN-PWY L-tryptophan biosynthesis
BioCyc:META_PWY-6629 superpathway of L-tryptophan biosynthesis
BioCyc:ECO_ALL-CHORISMATE-PWY superpathway of chorismate metabolism
BioCyc:ECO_TRPSYN-PWY L-tryptophan biosynthesis
BioCyc:ARA_COMPLETE-ARO-ARA-PWY superpathway of phenylalanine, tyrosine and tryptophan biosynthesis
BioCyc:ARA_TRPSYN-PWY L-tryptophan biosynthesis
BioCyc:VCHO_COMPLETE-ARO-PWY superpathway of aromatic amino acid biosynthesis
BioCyc:BSUB_PWY-6629 superpathway of L-tryptophan biosynthesis
BioCyc:SYNEL_PWY-6629 superpathway of L-tryptophan biosynthesis
BioCyc:SYNEL_TRPSYN-PWY L-tryptophan biosynthesis
BioCyc:CAULONA1000_COMPLETE-ARO-PWY superpathway of phenylalanine, tyrosine, and tryptophan biosynthesis
BioCyc:GCF_000013425_PWY-6629 superpathway of L-tryptophan biosynthesis
PathBank:SMP0000835 Tryptophan Metabolism
PathBank:SMP0000981 Trp Operon
PathBank:SMP0001930 Tryptophan Metabolism II
PlantCyc:ARA_COMPLETE-ARO-ARA-PWY superpathway of phenylalanine, tyrosine and tryptophan biosynthesis
PlantCyc:ARA_TRPSYN-PWY L-tryptophan biosynthesis
PlantCyc:PLANT_TRPSYN-PWY L-tryptophan biosynthesis
PathBank:SMP0002353 Tryptophan Metabolism