EC: 4.2.1.20

tryptophan synthase (L-serine hydro-lyase [adding 1-C-(indol-3-yl)glycerol 3-phosphate; L-tryptophan and glyceraldehyde-3-phosphate-forming])

enzyme lambda metabolic reaction experiment
uniprot:Q9WZ09 [tryptophan synthase(Enzyme) wildtype TrpB2]

L_Serine + Indole -> H2O + L_Tryptophan		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 100 mM Potassium phosphate, 180 mM KCl, 40 µM Pyridoxal 5'-phosphate
PH: 7.5
Temperature: 80
uniprot:Q9WZ09 [tryptophan synthase(Enzyme) wildtype TrpB2]

Indole + L_Serine -> L_Tryptophan + H2O		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 100 mM Potassium phosphate, 180 mM KCl, 40 µM Pyridoxal 5'-phosphate
PH: 7.5
Temperature: 80
- [tryptophan synthase(Enzyme) mutant alpha-subunit T63K]

(3_Indolyl)_glycerol_phosphate -> D_Glyceraldehyde_3_phosphate + Indole		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: Tris-Cl, 0.18 M NaCl, 0.2 mM dithiothreitol, 0.05 mM pyridoxal phosphate, 4 mM EDTA
PH: 7.8
Temperature: 37
- [tryptophan synthase(Enzyme) mutant alpha-subunit P62Q]

(3_Indolyl)_glycerol_phosphate -> D_Glyceraldehyde_3_phosphate + Indole		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: Tris-Cl, 0.18 M NaCl, 0.2 mM dithiothreitol, 0.05 mM pyridoxal phosphate, 4 mM EDTA
PH: 7.8
Temperature: 37
- [tryptophan synthase(Enzyme) mutant alpha-subunit G51D]

(3_Indolyl)_glycerol_phosphate -> D_Glyceraldehyde_3_phosphate + Indole		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: Tris-Cl, 0.18 M NaCl, 0.2 mM dithiothreitol, 0.05 mM pyridoxal phosphate, 4 mM EDTA
PH: 7.8
Temperature: 37
- [tryptophan synthase(Enzyme) mutant alpha-subunit Q65R]

(3_Indolyl)_glycerol_phosphate -> D_Glyceraldehyde_3_phosphate + Indole		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: Tris-Cl, 0.18 M NaCl, 0.2 mM dithiothreitol, 0.05 mM pyridoxal phosphate, 4 mM EDTA
PH: 7.8
Temperature: 37
- [tryptophan synthase(Enzyme) mutant alpha-subunit P28L]

(3_Indolyl)_glycerol_phosphate -> Indole + D_Glyceraldehyde_3_phosphate		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: Tris-Cl, 0.18 M NaCl, 0.2 mM dithiothreitol, 0.05 mM pyridoxal phosphate, 4 mM EDTA
PH: 7.8
Temperature: 37
- [tryptophan synthase(Enzyme) mutant alpha-subunit P53T]

(3_Indolyl)_glycerol_phosphate -> Indole + D_Glyceraldehyde_3_phosphate		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: Tris-Cl, 0.18 M NaCl, 0.2 mM dithiothreitol, 0.05 mM pyridoxal phosphate, 4 mM EDTA
PH: 7.8
Temperature: 37
- [tryptophan synthase(Enzyme) mutant alpha-subunit D56G]

(3_Indolyl)_glycerol_phosphate -> Indole + D_Glyceraldehyde_3_phosphate		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: Tris-Cl, 0.18 M NaCl, 0.2 mM dithiothreitol, 0.05 mM pyridoxal phosphate, 4 mM EDTA
PH: 7.8
Temperature: 37
- [tryptophan synthase(Enzyme) mutant alpha-subunit A67V]

(3_Indolyl)_glycerol_phosphate -> D_Glyceraldehyde_3_phosphate + Indole		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: Tris-Cl, 0.18 M NaCl, 0.2 mM dithiothreitol, 0.05 mM pyridoxal phosphate, 4 mM EDTA
PH: 7.8
Temperature: 37
- [tryptophan synthase(Enzyme) mutant alpha-subunit P53H]

(3_Indolyl)_glycerol_phosphate -> Indole + D_Glyceraldehyde_3_phosphate		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: Tris-Cl, 0.18 M NaCl, 0.2 mM dithiothreitol, 0.05 mM pyridoxal phosphate, 4 mM EDTA
PH: 7.8
Temperature: 37
- [tryptophan synthase(Enzyme) wildtype]

Indole + L_Serine -> H2O + L_Tryptophan		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: Tris-Cl, 0.5 mg/ml bovine serum albumine, 0.2 mM dithioerythritol, 4 mM EDTA
PH: 7.8
Temperature: 37
- [tryptophan synthase(Enzyme) mutant alpha-subunit D56G]

L_Serine + Indole -> H2O + L_Tryptophan		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: Tris-Cl, 0.5 mg/ml bovine serum albumine, 0.2 mM dithioerythritol, 4 mM EDTA
PH: 7.8
Temperature: 37
- [tryptophan synthase(Enzyme) mutant alpha-subunit G51D]

L_Serine + Indole -> H2O + L_Tryptophan		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: Tris-Cl, 0.5 mg/ml bovine serum albumine, 0.2 mM dithioerythritol, 4 mM EDTA
PH: 7.8
Temperature: 37
- [tryptophan synthase(Enzyme) mutant alpha-subunit P53H]

Indole + L_Serine -> H2O + L_Tryptophan		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: Tris-Cl, 0.5 mg/ml bovine serum albumine, 0.2 mM dithioerythritol, 4 mM EDTA
PH: 7.8
Temperature: 37
- [tryptophan synthase(Enzyme) mutant alpha-subunit P28L]

Indole + L_Serine -> H2O + L_Tryptophan		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: Tris-Cl, 0.5 mg/ml bovine serum albumine, 0.2 mM dithioerythritol, 4 mM EDTA
PH: 7.8
Temperature: 37
- [tryptophan synthase(Enzyme) mutant alpha-subunit P53T]

Indole + L_Serine -> L_Tryptophan + H2O		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: Tris-Cl, 0.5 mg/ml bovine serum albumine, 0.2 mM dithioerythritol, 4 mM EDTA
PH: 7.8
Temperature: 37
uniprot:P0A879 [tryptophan synthase(Enzyme) wildtype]

L_Serine + Indole -> H2O + L_Tryptophan		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: Tris-Cl, 0.5 mg/ml bovine serum albumine, 0.2 mM dithioerythritol, 4 mM EDTA
PH: 7.8
Temperature: 37
- [tryptophan synthase(Enzyme) mutant alpha-subunit Y115C]

(3_Indolyl)_glycerol_phosphate -> Indole + D_Glyceraldehyde_3_phosphate		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: Tris-Cl, 0.18 M NaCl, 0.2 mM dithiothreitol, 0.05 mM pyridoxal phosphate, 4 mM EDTA
PH: 7.8
Temperature: 37
- [tryptophan synthase(Enzyme) mutant alpha-subunit F114L]

(3_Indolyl)_glycerol_phosphate -> Indole + D_Glyceraldehyde_3_phosphate		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: Tris-Cl, 0.18 M NaCl, 0.2 mM dithiothreitol, 0.05 mM pyridoxal phosphate, 4 mM EDTA
PH: 7.8
Temperature: 37
- [tryptophan synthase(Enzyme) mutant alpha-subunit D56G]

L_Serine + (3_Indolyl)_glycerol_phosphate -> L_Tryptophan + H2O + D_Glyceraldehyde_3_phosphate		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: Tris-Cl
PH: 7.8
Temperature: 37
- [tryptophan synthase(Enzyme) mutant alpha-subunit C81R]

L_Serine + (3_Indolyl)_glycerol_phosphate -> L_Tryptophan + H2O + D_Glyceraldehyde_3_phosphate		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: Tris-Cl
PH: 7.8
Temperature: 37
- [tryptophan synthase(Enzyme) mutant alpha-subunit Y102C]

L_Serine + (3_Indolyl)_glycerol_phosphate -> D_Glyceraldehyde_3_phosphate + H2O + L_Tryptophan		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: Tris-Cl
PH: 7.8
Temperature: 37
- [tryptophan synthase(Enzyme) mutant alpha-subunit N104S]

(3_Indolyl)_glycerol_phosphate + L_Serine -> H2O + L_Tryptophan + D_Glyceraldehyde_3_phosphate		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: Tris-Cl
PH: 7.8
Temperature: 37
- [tryptophan synthase(Enzyme) mutant alpha-subunit S6P]

L_Serine + (3_Indolyl)_glycerol_phosphate -> L_Tryptophan + D_Glyceraldehyde_3_phosphate + H2O		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: Tris-Cl
PH: 7.8
Temperature: 37
- [tryptophan synthase(Enzyme) mutant alpha-subunit A67V]

(3_Indolyl)_glycerol_phosphate + L_Serine -> H2O + L_Tryptophan + D_Glyceraldehyde_3_phosphate		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: Tris-Cl
PH: 7.8
Temperature: 37
- [tryptophan synthase(Enzyme) mutant alpha-subunit Q65R]

L_Serine + (3_Indolyl)_glycerol_phosphate -> H2O + D_Glyceraldehyde_3_phosphate + L_Tryptophan		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: Tris-Cl
PH: 7.8
Temperature: 37
- [tryptophan synthase(Enzyme) mutant alpha-subunit Y102H]

L_Serine + (3_Indolyl)_glycerol_phosphate -> H2O + D_Glyceraldehyde_3_phosphate + L_Tryptophan		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: Tris-Cl
PH: 7.8
Temperature: 37
- [tryptophan synthase(Enzyme) mutant alpha-subunit T63K]

L_Serine + (3_Indolyl)_glycerol_phosphate -> D_Glyceraldehyde_3_phosphate + L_Tryptophan + H2O		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: Tris-Cl
PH: 7.8
Temperature: 37
- [tryptophan synthase(Enzyme) wildtype]

(3_Indolyl)_glycerol_phosphate -> Indole + D_Glyceraldehyde_3_phosphate		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: Tris-Cl, 0.18 M NaCl, 0.2 mM dithiothreitol, 0.05 mM pyridoxal phosphate, 4 mM EDTA
PH: 7.8
Temperature: 37
- [tryptophan synthase(Enzyme) mutant alpha-subunit Y4C]

(3_Indolyl)_glycerol_phosphate -> D_Glyceraldehyde_3_phosphate + Indole		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: Tris-Cl, 0.18 M NaCl, 0.2 mM dithiothreitol, 0.05 mM pyridoxal phosphate, 4 mM EDTA
PH: 7.8
Temperature: 37
uniprot:P0A877 [tryptophan synthase(Enzyme) wildtype]

(3_Indolyl)_glycerol_phosphate -> Indole + D_Glyceraldehyde_3_phosphate		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: Tris-Cl, 0.18 M NaCl, 0.2 mM dithiothreitol, 0.05 mM pyridoxal phosphate, 4 mM EDTA
PH: 7.8
Temperature: 37
- [tryptophan synthase(Enzyme) mutant alpha-subunit F22S]

(3_Indolyl)_glycerol_phosphate -> Indole + D_Glyceraldehyde_3_phosphate		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: Tris-Cl, 0.18 M NaCl, 0.2 mM dithiothreitol, 0.05 mM pyridoxal phosphate, 4 mM EDTA
PH: 7.8
Temperature: 37
- [tryptophan synthase(Enzyme) mutant alpha-subunit D112N]

(3_Indolyl)_glycerol_phosphate -> D_Glyceraldehyde_3_phosphate + Indole		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: Tris-Cl, 0.18 M NaCl, 0.2 mM dithiothreitol, 0.05 mM pyridoxal phosphate, 4 mM EDTA
PH: 7.8
Temperature: 37
- [tryptophan synthase(Enzyme) mutant alpha-subunit P78L]

(3_Indolyl)_glycerol_phosphate -> Indole + D_Glyceraldehyde_3_phosphate		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: Tris-Cl, 0.18 M NaCl, 0.2 mM dithiothreitol, 0.05 mM pyridoxal phosphate, 4 mM EDTA
PH: 7.8
Temperature: 37
- [tryptophan synthase(Enzyme) mutant alpha-subunit M101I]

(3_Indolyl)_glycerol_phosphate -> D_Glyceraldehyde_3_phosphate + Indole		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: Tris-Cl, 0.18 M NaCl, 0.2 mM dithiothreitol, 0.05 mM pyridoxal phosphate, 4 mM EDTA
PH: 7.8
Temperature: 37
- [tryptophan synthase(Enzyme) mutant alpha-subunit D112G]

(3_Indolyl)_glycerol_phosphate -> Indole + D_Glyceraldehyde_3_phosphate		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: Tris-Cl, 0.18 M NaCl, 0.2 mM dithiothreitol, 0.05 mM pyridoxal phosphate, 4 mM EDTA
PH: 7.8
Temperature: 37
- [tryptophan synthase(Enzyme) mutant alpha-subunit P78S]

(3_Indolyl)_glycerol_phosphate -> D_Glyceraldehyde_3_phosphate + Indole		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: Tris-Cl, 0.18 M NaCl, 0.2 mM dithiothreitol, 0.05 mM pyridoxal phosphate, 4 mM EDTA
PH: 7.8
Temperature: 37
- [tryptophan synthase(Enzyme) mutant alpha-subunit M101T]

(3_Indolyl)_glycerol_phosphate -> Indole + D_Glyceraldehyde_3_phosphate		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: Tris-Cl, 0.18 M NaCl, 0.2 mM dithiothreitol, 0.05 mM pyridoxal phosphate, 4 mM EDTA
PH: 7.8
Temperature: 37
- [tryptophan synthase(Enzyme) mutant alpha-subunit G44S]

(3_Indolyl)_glycerol_phosphate -> Indole + D_Glyceraldehyde_3_phosphate		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: Tris-Cl, 0.18 M NaCl, 0.2 mM dithiothreitol, 0.05 mM pyridoxal phosphate, 4 mM EDTA
PH: 7.8
Temperature: 37
- [tryptophan synthase(Enzyme) mutant alpha-subunit P98S]

(3_Indolyl)_glycerol_phosphate -> D_Glyceraldehyde_3_phosphate + Indole		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: Tris-Cl, 0.18 M NaCl, 0.2 mM dithiothreitol, 0.05 mM pyridoxal phosphate, 4 mM EDTA
PH: 7.8
Temperature: 37
- [tryptophan synthase(Enzyme) mutant alpha-subunit M101V]

(3_Indolyl)_glycerol_phosphate -> Indole + D_Glyceraldehyde_3_phosphate		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: Tris-Cl, 0.18 M NaCl, 0.2 mM dithiothreitol, 0.05 mM pyridoxal phosphate, 4 mM EDTA
PH: 7.8
Temperature: 37
- [tryptophan synthase(Enzyme) wildtype]

L_Serine + (3_Indolyl)_glycerol_phosphate -> H2O + L_Tryptophan + D_Glyceraldehyde_3_phosphate		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: Tris-Cl
PH: 7.8
Temperature: 37
- [tryptophan synthase(Enzyme) mutant alpha-subunit Y4C]

(3_Indolyl)_glycerol_phosphate + L_Serine -> D_Glyceraldehyde_3_phosphate + L_Tryptophan + H2O		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: Tris-Cl
PH: 7.8
Temperature: 37
- [tryptophan synthase(Enzyme) mutant alpha-subunit F22S]

(3_Indolyl)_glycerol_phosphate + L_Serine -> L_Tryptophan + H2O + D_Glyceraldehyde_3_phosphate		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: Tris-Cl
PH: 7.8
Temperature: 37
- [tryptophan synthase(Enzyme) mutant alpha-subunit P28L]

L_Serine + (3_Indolyl)_glycerol_phosphate -> H2O + D_Glyceraldehyde_3_phosphate + L_Tryptophan		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: Tris-Cl
PH: 7.8
Temperature: 37
- [tryptophan synthase(Enzyme) mutant alpha-subunit P53T]

L_Serine + (3_Indolyl)_glycerol_phosphate -> D_Glyceraldehyde_3_phosphate + H2O + L_Tryptophan		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: Tris-Cl
PH: 7.8
Temperature: 37
- [tryptophan synthase(Enzyme) mutant alpha-subunit P53H]

(3_Indolyl)_glycerol_phosphate + L_Serine -> H2O + D_Glyceraldehyde_3_phosphate + L_Tryptophan		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: Tris-Cl
PH: 7.8
Temperature: 37
- [tryptophan synthase(Enzyme) mutant alpha-subunit P62Q]

(3_Indolyl)_glycerol_phosphate + L_Serine -> H2O + L_Tryptophan + D_Glyceraldehyde_3_phosphate		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: Tris-Cl
PH: 7.8
Temperature: 37
- [tryptophan synthase(Enzyme) mutant alpha-subunit G51D]

(3_Indolyl)_glycerol_phosphate + L_Serine -> L_Tryptophan + D_Glyceraldehyde_3_phosphate + H2O		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: Tris-Cl
PH: 7.8
Temperature: 37
- [tryptophan synthase(Enzyme) mutant alpha-subunit P98S]

Indole + L_Serine -> L_Tryptophan + H2O		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: Tris-Cl, 0.5 mg/ml bovine serum albumine, 0.2 mM dithioerythritol, 4 mM EDTA
PH: 7.8
Temperature: 37
- [tryptophan synthase(Enzyme) mutant alpha-subunit P98S]

(3_Indolyl)_glycerol_phosphate + L_Serine -> L_Tryptophan + H2O + D_Glyceraldehyde_3_phosphate		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: Tris-Cl
PH: 7.8
Temperature: 37
- [tryptophan synthase(Enzyme) mutant alpha-subunit M101V]

(3_Indolyl)_glycerol_phosphate + L_Serine -> H2O + L_Tryptophan + D_Glyceraldehyde_3_phosphate		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: Tris-Cl
PH: 7.8
Temperature: 37
- [tryptophan synthase(Enzyme) mutant alpha-subunit F114L]

(3_Indolyl)_glycerol_phosphate + L_Serine -> H2O + L_Tryptophan + D_Glyceraldehyde_3_phosphate		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: Tris-Cl
PH: 7.8
Temperature: 37
- [tryptophan synthase(Enzyme) mutant alpha-subunit M101I]

L_Serine + (3_Indolyl)_glycerol_phosphate -> D_Glyceraldehyde_3_phosphate + H2O + L_Tryptophan		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: Tris-Cl
PH: 7.8
Temperature: 37
- [tryptophan synthase(Enzyme) mutant alpha-subunit Y115C]

(3_Indolyl)_glycerol_phosphate + L_Serine -> L_Tryptophan + H2O + D_Glyceraldehyde_3_phosphate		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: Tris-Cl
PH: 7.8
Temperature: 37
- [tryptophan synthase(Enzyme) mutant alpha-subunit M101T]

(3_Indolyl)_glycerol_phosphate + L_Serine -> H2O + D_Glyceraldehyde_3_phosphate + L_Tryptophan		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: Tris-Cl
PH: 7.8
Temperature: 37
- [tryptophan synthase(Enzyme) mutant alpha-subunit D112G]

L_Serine + (3_Indolyl)_glycerol_phosphate -> L_Tryptophan + H2O + D_Glyceraldehyde_3_phosphate		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: Tris-Cl
PH: 7.8
Temperature: 37
- [tryptophan synthase(Enzyme) mutant alpha-subunit D112N]

L_Serine + (3_Indolyl)_glycerol_phosphate -> L_Tryptophan + D_Glyceraldehyde_3_phosphate + H2O		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: Tris-Cl
PH: 7.8
Temperature: 37
- [tryptophan synthase(Enzyme) mutant alpha-subunit C81R]

(3_Indolyl)_glycerol_phosphate -> D_Glyceraldehyde_3_phosphate + Indole		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: Tris-Cl, 0.18 M NaCl, 0.2 mM dithiothreitol, 0.05 mM pyridoxal phosphate, 4 mM EDTA
PH: 7.8
Temperature: 37
- [tryptophan synthase(Enzyme) mutant alpha-subunit Y102C]

(3_Indolyl)_glycerol_phosphate -> D_Glyceraldehyde_3_phosphate + Indole		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: Tris-Cl, 0.18 M NaCl, 0.2 mM dithiothreitol, 0.05 mM pyridoxal phosphate, 4 mM EDTA
PH: 7.8
Temperature: 37
- [tryptophan synthase(Enzyme) mutant alpha-subunit P21S]

(3_Indolyl)_glycerol_phosphate -> Indole + D_Glyceraldehyde_3_phosphate		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: Tris-Cl, 0.18 M NaCl, 0.2 mM dithiothreitol, 0.05 mM pyridoxal phosphate, 4 mM EDTA
PH: 7.8
Temperature: 37
- [tryptophan synthase(Enzyme) mutant alpha-subunit N104S]

(3_Indolyl)_glycerol_phosphate -> D_Glyceraldehyde_3_phosphate + Indole		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: Tris-Cl, 0.18 M NaCl, 0.2 mM dithiothreitol, 0.05 mM pyridoxal phosphate, 4 mM EDTA
PH: 7.8
Temperature: 37
- [tryptophan synthase(Enzyme) mutant alpha-subunit T24M]

(3_Indolyl)_glycerol_phosphate -> Indole + D_Glyceraldehyde_3_phosphate		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: Tris-Cl, 0.18 M NaCl, 0.2 mM dithiothreitol, 0.05 mM pyridoxal phosphate, 4 mM EDTA
PH: 7.8
Temperature: 37
- [tryptophan synthase(Enzyme) mutant alpha-subunit P28S]

(3_Indolyl)_glycerol_phosphate -> D_Glyceraldehyde_3_phosphate + Indole		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: Tris-Cl, 0.18 M NaCl, 0.2 mM dithiothreitol, 0.05 mM pyridoxal phosphate, 4 mM EDTA
PH: 7.8
Temperature: 37
- [tryptophan synthase(Enzyme) mutant alpha-subunit S33L]

(3_Indolyl)_glycerol_phosphate -> D_Glyceraldehyde_3_phosphate + Indole		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: Tris-Cl, 0.18 M NaCl, 0.2 mM dithiothreitol, 0.05 mM pyridoxal phosphate, 4 mM EDTA
PH: 7.8
Temperature: 37
- [tryptophan synthase(Enzyme) mutant alpha-subunit Y102H]

(3_Indolyl)_glycerol_phosphate -> D_Glyceraldehyde_3_phosphate + Indole		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: Tris-Cl, 0.18 M NaCl, 0.2 mM dithiothreitol, 0.05 mM pyridoxal phosphate, 4 mM EDTA
PH: 7.8
Temperature: 37
- [tryptophan synthase(Enzyme) mutant alpha-subunit S6P]

(3_Indolyl)_glycerol_phosphate -> D_Glyceraldehyde_3_phosphate + Indole		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: Tris-Cl, 0.18 M NaCl, 0.2 mM dithiothreitol, 0.05 mM pyridoxal phosphate, 4 mM EDTA
PH: 7.8
Temperature: 37
- [tryptophan synthase(Enzyme) mutant alpha-subunit P62Q]

L_Serine + Indole -> L_Tryptophan + H2O		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: Tris-Cl, 0.5 mg/ml bovine serum albumine, 0.2 mM dithioerythritol, 4 mM EDTA
PH: 7.8
Temperature: 37
- [tryptophan synthase(Enzyme) mutant alpha-subunit P28S]

L_Serine + Indole -> L_Tryptophan + H2O		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: Tris-Cl, 0.5 mg/ml bovine serum albumine, 0.2 mM dithioerythritol, 4 mM EDTA
PH: 7.8
Temperature: 37
- [tryptophan synthase(Enzyme) mutant alpha-subunit P21S]

L_Serine + Indole -> L_Tryptophan + H2O		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: Tris-Cl, 0.5 mg/ml bovine serum albumine, 0.2 mM dithioerythritol, 4 mM EDTA
PH: 7.8
Temperature: 37
- [tryptophan synthase(Enzyme) mutant alpha-subunit Y102H]

Indole + L_Serine -> L_Tryptophan + H2O		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: Tris-Cl, 0.5 mg/ml bovine serum albumine, 0.2 mM dithioerythritol, 4 mM EDTA
PH: 7.8
Temperature: 37
- [tryptophan synthase(Enzyme) mutant alpha-subunit A67V]

L_Serine + Indole -> L_Tryptophan + H2O		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: Tris-Cl, 0.5 mg/ml bovine serum albumine, 0.2 mM dithioerythritol, 4 mM EDTA
PH: 7.8
Temperature: 37
- [tryptophan synthase(Enzyme) mutant alpha-subunit Y102C]

L_Serine + Indole -> H2O + L_Tryptophan		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: Tris-Cl, 0.5 mg/ml bovine serum albumine, 0.2 mM dithioerythritol, 4 mM EDTA
PH: 7.8
Temperature: 37
- [tryptophan synthase(Enzyme) mutant alpha-subunit Q65R]

L_Serine + Indole -> H2O + L_Tryptophan		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: Tris-Cl, 0.5 mg/ml bovine serum albumine, 0.2 mM dithioerythritol, 4 mM EDTA
PH: 7.8
Temperature: 37
- [tryptophan synthase(Enzyme) mutant alpha-subunit N104S]

L_Serine + Indole -> L_Tryptophan + H2O		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: Tris-Cl, 0.5 mg/ml bovine serum albumine, 0.2 mM dithioerythritol, 4 mM EDTA
PH: 7.8
Temperature: 37
- [tryptophan synthase(Enzyme) mutant alpha-subunit T63K]

Indole + L_Serine -> H2O + L_Tryptophan		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: Tris-Cl, 0.5 mg/ml bovine serum albumine, 0.2 mM dithioerythritol, 4 mM EDTA
PH: 7.8
Temperature: 37
- [tryptophan synthase(Enzyme) mutant alpha-subunit S33L]

L_Serine + (3_Indolyl)_glycerol_phosphate -> H2O + D_Glyceraldehyde_3_phosphate + L_Tryptophan		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: Tris-Cl
PH: 7.8
Temperature: 37
- [tryptophan synthase(Enzyme) mutant alpha-subunit G44S]

(3_Indolyl)_glycerol_phosphate + L_Serine -> L_Tryptophan + D_Glyceraldehyde_3_phosphate + H2O		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: Tris-Cl
PH: 7.8
Temperature: 37
- [tryptophan synthase(Enzyme) mutant alpha-subunit P78S]

L_Serine + (3_Indolyl)_glycerol_phosphate -> H2O + D_Glyceraldehyde_3_phosphate + L_Tryptophan		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: Tris-Cl
PH: 7.8
Temperature: 37
- [tryptophan synthase(Enzyme) mutant alpha-subunit D46N]

(3_Indolyl)_glycerol_phosphate + L_Serine -> D_Glyceraldehyde_3_phosphate + H2O + L_Tryptophan		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: Tris-Cl
PH: 7.8
Temperature: 37
- [tryptophan synthase(Enzyme) mutant alpha-subunit P21S]

(3_Indolyl)_glycerol_phosphate + L_Serine -> L_Tryptophan + D_Glyceraldehyde_3_phosphate + H2O		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: Tris-Cl
PH: 7.8
Temperature: 37
- [tryptophan synthase(Enzyme) mutant alpha-subunit T24M]

(3_Indolyl)_glycerol_phosphate + L_Serine -> D_Glyceraldehyde_3_phosphate + L_Tryptophan + H2O		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: Tris-Cl
PH: 7.8
Temperature: 37
- [tryptophan synthase(Enzyme) mutant alpha-subunit D46G]

(3_Indolyl)_glycerol_phosphate + L_Serine -> L_Tryptophan + H2O + D_Glyceraldehyde_3_phosphate		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: Tris-Cl
PH: 7.8
Temperature: 37
- [tryptophan synthase(Enzyme) mutant alpha-subunit P78L]

(3_Indolyl)_glycerol_phosphate + L_Serine -> H2O + L_Tryptophan + D_Glyceraldehyde_3_phosphate		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: Tris-Cl
PH: 7.8
Temperature: 37
- [tryptophan synthase(Enzyme) mutant alpha-subunit P28S]

L_Serine + (3_Indolyl)_glycerol_phosphate -> L_Tryptophan + H2O + D_Glyceraldehyde_3_phosphate		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: Tris-Cl
PH: 7.8
Temperature: 37

Pathways

pathway id name
BioCyc:META_ALL-CHORISMATE-PWY superpathway of chorismate metabolism
BioCyc:ECO_COMPLETE-ARO-PWY superpathway of aromatic amino acid biosynthesis
BioCyc:YEAST_TRPSYN-PWY tryptophan biosynthesis
BioCyc:MTBH37RV_PWY-6629 superpathway of L-tryptophan biosynthesis
BioCyc:MTBCDC1551_TRPSYN-PWY L-tryptophan biosynthesis
BioCyc:ECOL199310_COMPLETE-ARO-PWY superpathway of phenylalanine, tyrosine, and tryptophan biosynthesis
BioCyc:CAULO_COMPLETE-ARO-PWY superpathway of phenylalanine, tyrosine, and tryptophan biosynthesis
BioCyc:BSUB_TRPSYN-PWY L-tryptophan biosynthesis
BioCyc:ECOL316407_TRPSYN-PWY tryptophan biosynthesis
BioCyc:ECOL316407_ALL-CHORISMATE-PWY superpathway of chorismate metabolism
BioCyc:BTHE_TRPSYN-PWY tryptophan biosynthesis
BioCyc:CORYNE_PWY-6629 superpathway of L-tryptophan biosynthesis
BioCyc:GCF_000013425_TRPSYN-PWY L-tryptophan biosynthesis
BioCyc:META_PWY-6949 DIBOA-glucoside biosynthesis
BioCyc:VCHO_ALL-CHORISMATE-PWY superpathway of chorismate metabolism
BioCyc:VCHO_PWY-6629 superpathway of L-tryptophan biosynthesis
BioCyc:HPY_TRPSYN-PWY L-tryptophan biosynthesis
BioCyc:ECOO157_COMPLETE-ARO-PWY superpathway of phenylalanine, tyrosine, and tryptophan biosynthesis
BioCyc:ECOO157_PWY-6629 superpathway of tryptophan biosynthesis
BioCyc:ECOL199310_TRPSYN-PWY tryptophan biosynthesis
BioCyc:CAULO_TRPSYN-PWY tryptophan biosynthesis
BioCyc:ANTHRA_TRPSYN-PWY L-tryptophan biosynthesis
BioCyc:AGRO_TRPSYN-PWY L-tryptophan biosynthesis
BioCyc:AGRO_COMPLETE-ARO-PWY superpathway of aromatic amino acid biosynthesis
BioCyc:ECOL316407_COMPLETE-ARO-PWY superpathway of phenylalanine, tyrosine, and tryptophan biosynthesis
BioCyc:CORYNE_TRPSYN-PWY L-tryptophan biosynthesis
BioCyc:META_COMPLETE-ARO-PWY superpathway of aromatic amino acid biosynthesis
BioCyc:YEAST_COMPLETE-ARO-YEAST-PWY superpathway of phenylalanine, tyrosine and tryptophan biosynthesis
BioCyc:VCHO_TRPSYN-PWY L-tryptophan biosynthesis
BioCyc:MTBH37RV_TRPSYN-PWY L-tryptophan biosynthesis
BioCyc:ECOO157_TRPSYN-PWY tryptophan biosynthesis
BioCyc:ECOL199310_PWY-6629 superpathway of tryptophan biosynthesis
BioCyc:CAULONA1000_TRPSYN-PWY tryptophan biosynthesis
BioCyc:CORYNE_COMPLETE-ARO-PWY superpathway of aromatic amino acid biosynthesis
BioCyc:META_PWY-4161 superpathway of benzoxazinoid glucosides biosynthesis
BioCyc:META_TRPSYN-PWY L-tryptophan biosynthesis
BioCyc:META_PWY-6629 superpathway of L-tryptophan biosynthesis
BioCyc:ECO_ALL-CHORISMATE-PWY superpathway of chorismate metabolism
BioCyc:ECO_TRPSYN-PWY L-tryptophan biosynthesis
BioCyc:ARA_COMPLETE-ARO-ARA-PWY superpathway of phenylalanine, tyrosine and tryptophan biosynthesis
BioCyc:ARA_TRPSYN-PWY L-tryptophan biosynthesis
BioCyc:VCHO_COMPLETE-ARO-PWY superpathway of aromatic amino acid biosynthesis
BioCyc:ECOL199310_ALL-CHORISMATE-PWY superpathway of chorismate metabolism
BioCyc:ANTHRA_COMPLETE-ARO-PWY superpathway of aromatic amino acid biosynthesis
BioCyc:BSUB_PWY-6629 superpathway of L-tryptophan biosynthesis
BioCyc:SYNEL_PWY-6629 superpathway of L-tryptophan biosynthesis
BioCyc:SYNEL_TRPSYN-PWY L-tryptophan biosynthesis
BioCyc:CAULONA1000_COMPLETE-ARO-PWY superpathway of phenylalanine, tyrosine, and tryptophan biosynthesis
BioCyc:GCF_000013425_PWY-6629 superpathway of L-tryptophan biosynthesis
PathBank:SMP0000835 Tryptophan Metabolism
PathBank:SMP0000981 Trp Operon
PathBank:SMP0001930 Tryptophan Metabolism II
PlantCyc:ARA_COMPLETE-ARO-ARA-PWY superpathway of phenylalanine, tyrosine and tryptophan biosynthesis
PlantCyc:CORN_PWY-6949 DIBOA-glucoside biosynthesis
PlantCyc:PLANT_PWY-4161 superpathway of benzoxazinoid glucosides biosynthesis
PlantCyc:ARA_TRPSYN-PWY L-tryptophan biosynthesis
PlantCyc:CORN_TRPSYN-PWY L-tryptophan biosynthesis
PlantCyc:PLANT_PWY-6949 DIBOA-glucoside biosynthesis
PlantCyc:PLANT_TRPSYN-PWY L-tryptophan biosynthesis
PathBank:SMP0002353 Tryptophan Metabolism
PathBank:SMP0002445 Tryptophan Metabolism