EC: 3.1.4.17

3',5'-cyclic-nucleotide phosphodiesterase (3',5'-cyclic-nucleotide 5'-nucleotidohydrolase)

enzyme lambda metabolic reaction experiment
- [3',5'-cyclic-nucleotide phosphodiesterase(Enzyme) wildtype high-affinity component]

H2O + 3',5'_Cyclic_AMP -> AMP		 (( (Vmax * S) ) / (Km + S)) buffer: 40 mM Tris-Cl, 5 mM MgCl2, 3.75 mM 2-Mercaptoethanol
PH: 8
Temperature: 30
- [3',5'-cyclic-nucleotide phosphodiesterase(Enzyme) wildtype low-affinity component]

3',5'_Cyclic_AMP + H2O -> AMP		 (( (Vmax * S) ) / (Km + S)) buffer: 40 mM Tris-Cl, 5 mM MgCl2, 3.75 mM 2-Mercaptoethanol
PH: 8
Temperature: 30
- [3',5'-cyclic-nucleotide phosphodiesterase(Enzyme) wildtype]

3',5'_Cyclic_GMP + H2O -> GMP		 (( (Vmax * S) ) / (Km + S)) buffer: 40 mM Tris-Cl, 5 mM MgCl2, 3.75 mM 2-Mercaptoethanol
PH: 8
Temperature: 30
uniprot:Q9Y233 [3',5'-cyclic-nucleotide phosphodiesterase(Enzyme) wildtype]

H2O + 3',5'_Cyclic_AMP -> AMP		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 5 mM MgCl2, 4 mM 2-mercaptoethanol, 0.33 mg/ml bovine serum albumin
PH: 8
Temperature: 37
- [Mg2+]

H2O + 3',5'_Cyclic_AMP -> AMP		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris/HCl
PH: 7.5
Temperature: 30
uniprot:Q9QYJ6 [3',5'-cyclic-nucleotide phosphodiesterase(Enzyme) wildtype]

H2O + 3',5'_Cyclic_AMP -> AMP		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 5 mM MgCl2, 4 mM 2-mercaptoethanol, 0.33 mg/ml bovine serum albumin
PH: 8
Temperature: 37
uniprot:Q9QYJ6 [3',5'-cyclic-nucleotide phosphodiesterase(Enzyme) wildtype]

3',5'_Cyclic_GMP + H2O -> GMP		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 5 mM MgCl2, 4 mM 2-mercaptoethanol, 0.33 mg/ml bovine serum albumin
PH: 8
Temperature: 37
uniprot:Q5SM25 [Mn2+]

H2O + 3',5'_Cyclic_AMP -> AMP		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 100 mM KCl
PH: 7.5
Temperature: 37
uniprot:Q5SM25 [Mn2+]

H2O + 3',5'_Cyclic_GMP -> GMP		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 100 mM KCl
PH: 7.5
Temperature: 37
uniprot:O25683 [3',5'-cyclic-nucleotide phosphodiesterase(Enzyme) wildtype]

3',5'_Cyclic_AMP + H2O -> AMP		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 100 mM sodium citrate, 0.1 mM MnCl2
PH: 4.5
Temperature: 30
uniprot:O25683 [3',5'-cyclic-nucleotide phosphodiesterase(Enzyme) wildtype]

H2O + 3',5'_Cyclic_GMP -> GMP		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 100 mM sodium citrate, 0.1 mM MnCl2
PH: 4.5
Temperature: 30
uniprot:O25683 [3',5'-cyclic-nucleotide phosphodiesterase(Enzyme) wildtype]

Bis_4_nitrophenyl_phosphate + H2O -> 4_Nitrophenyl_phosphate + p_Nitrophenol		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 100 mM sodium citrate, 1 mM MnCl2
PH: 4.5
Temperature: 30
uniprot:Q8CA95 [3',5'-cyclic-nucleotide phosphodiesterase(Enzyme) wildtype]

H2O + 3',5'_Cyclic_AMP -> AMP		 (( (Vmax * S) ) / (Km + S)) buffer: 40 mM Mops, 0.8 mM EGTA, 15 mM Magnesium acetate, 0.2 mg/ml BSA
PH: 7.5
Temperature: 36
uniprot:Q9Y233 [Mg2+]

H2O + 3',5'_Cyclic_AMP -> AMP		 (( (Vmax * S) ) / (Km + S)) buffer: 20 mM Tris-HCl, 1.0 mM DTT, 10 mM MgCl2
PH: 7.5
Temperature: 22
uniprot:Q14432 [3',5'-cyclic-nucleotide phosphodiesterase(Enzyme) mutant C816S]

H2O + 3',5'_Cyclic_AMP -> AMP		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 10 mM MgCl2
PH: 7.8
Temperature: 30
uniprot:Q14432 [3',5'-cyclic-nucleotide phosphodiesterase(Enzyme) mutant D811A]

3',5'_Cyclic_AMP + H2O -> AMP		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 10 mM MgCl2
PH: 7.8
Temperature: 30
uniprot:Q14432 [3',5'-cyclic-nucleotide phosphodiesterase(Enzyme) mutant Y807A]

H2O + 3',5'_Cyclic_AMP -> AMP		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 10 mM MgCl2
PH: 7.8
Temperature: 30
uniprot:Q14432 [3',5'-cyclic-nucleotide phosphodiesterase(Enzyme) mutant T810A]

3',5'_Cyclic_AMP + H2O -> AMP		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 10 mM MgCl2
PH: 7.8
Temperature: 30
uniprot:Q14432 [3',5'-cyclic-nucleotide phosphodiesterase(Enzyme) mutant Y814A]

H2O + 3',5'_Cyclic_AMP -> AMP		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 10 mM MgCl2
PH: 7.8
Temperature: 30
uniprot:Q14432 [3',5'-cyclic-nucleotide phosphodiesterase(Enzyme) mutant G815A]

H2O + 3',5'_Cyclic_AMP -> AMP		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 10 mM MgCl2
PH: 7.8
Temperature: 30
uniprot:Q14432 [3',5'-cyclic-nucleotide phosphodiesterase(Enzyme) mutant Y807C]

3',5'_Cyclic_AMP + H2O -> AMP		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 10 mM MgCl2
PH: 7.8
Temperature: 30
uniprot:Q14432 [3',5'-cyclic-nucleotide phosphodiesterase(Enzyme) mutant D812A]

3',5'_Cyclic_AMP + H2O -> AMP		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 10 mM MgCl2
PH: 7.8
Temperature: 30
uniprot:Q14432 [3',5'-cyclic-nucleotide phosphodiesterase(Enzyme) mutant K805A]

3',5'_Cyclic_AMP + H2O -> AMP		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 10 mM MgCl2
PH: 7.8
Temperature: 30
uniprot:Q14432 [3',5'-cyclic-nucleotide phosphodiesterase(Enzyme) wildtype]

3',5'_Cyclic_AMP + H2O -> AMP		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 10 mM MgCl2
PH: 7.8
Temperature: 30
uniprot:Q14432 [3',5'-cyclic-nucleotide phosphodiesterase(Enzyme) mutant S804A]

3',5'_Cyclic_AMP + H2O -> AMP		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 10 mM MgCl2
PH: 7.8
Temperature: 30
uniprot:Q14432 [3',5'-cyclic-nucleotide phosphodiesterase(Enzyme) mutant H798A]

3',5'_Cyclic_AMP + H2O -> AMP		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 10 mM MgCl2
PH: 7.8
Temperature: 30
uniprot:Q14432 [3',5'-cyclic-nucleotide phosphodiesterase(Enzyme) mutant H796A]

3',5'_Cyclic_AMP + H2O -> AMP		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 10 mM MgCl2
PH: 7.8
Temperature: 30
uniprot:Q14432 [3',5'-cyclic-nucleotide phosphodiesterase(Enzyme) mutant H782A]

H2O + 3',5'_Cyclic_AMP -> AMP		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 10 mM MgCl2
PH: 7.8
Temperature: 30
- [3',5'-cyclic-nucleotide phosphodiesterase(Enzyme) wildtype]

3',5'_Cyclic_AMP + H2O -> AMP		 (( (Vmax * S) ) / (Km + S)) buffer: 40 mM Tris-HCl, 5 mM MgCl2, 3.75 mM 2-Mercaptoethanol
PH: 7.4
Temperature: 30
- [3',5'-cyclic-nucleotide phosphodiesterase(Enzyme) wildtype]

3',5'_Cyclic_AMP + H2O -> AMP		 (( (Vmax * S) ) / (Km + S)) buffer: 40 mM Tris-HCl, 5 mM MgCl2, 3.75 mM 2-Mercaptoethanol
PH: 7.4
Temperature: 30

Pathways

pathway id name
Reactome:R-BTA-418594 G alpha (i) signalling events
Reactome:R-BTA-112043 PLC beta mediated events
Reactome:R-BTA-111933 Calmodulin induced events
Reactome:R-BTA-111957 Cam-PDE 1 activation
Reactome:R-BTA-1489509 DAG and IP3 signaling
Reactome:R-CEL-112399 IRS-mediated signalling
Reactome:R-CEL-165160 PDE3B signalling
Reactome:R-CEL-2428928 IRS-related events triggered by IGF1R
Reactome:R-CEL-388396 GPCR downstream signalling
Reactome:R-CEL-111997 CaM pathway
Reactome:R-CEL-111957 Cam-PDE 1 activation
Reactome:R-CEL-9006925 Intracellular signaling by second messengers
Reactome:R-DME-112040 G-protein mediated events
Reactome:R-DME-111933 Calmodulin induced events
Reactome:R-HSA-109703 PKB-mediated events
Reactome:R-HSA-2428924 IGF1R signaling cascade
Reactome:R-HSA-112040 G-protein mediated events
Reactome:R-HSA-111997 CaM pathway
Reactome:R-HSA-9006925 Intracellular signaling by second messengers
Reactome:R-HSA-1489509 DAG and IP3 signaling
Reactome:R-MMU-162582 Signaling Pathways
Reactome:R-MMU-112399 IRS-mediated signalling
Reactome:R-MMU-165160 PDE3B signalling
Reactome:R-MMU-2428928 IRS-related events triggered by IGF1R
Reactome:R-MMU-388396 GPCR downstream signalling
Reactome:R-MMU-111885 Opioid Signalling
Reactome:R-MMU-111996 Ca-dependent events
Reactome:R-MMU-1489509 DAG and IP3 signaling
Reactome:R-RNO-162582 Signaling Pathways
Reactome:R-RNO-74752 Signaling by Insulin receptor
Reactome:R-RNO-109704 PI3K Cascade
Reactome:R-RNO-2404192 Signaling by Type 1 Insulin-like Growth Factor 1 Receptor (IGF1R)
Reactome:R-RNO-372790 Signaling by GPCR
Reactome:R-RNO-418555 G alpha (s) signalling events
Reactome:R-BTA-162582 Signaling Pathways
Reactome:R-BTA-372790 Signaling by GPCR
Reactome:R-BTA-112040 G-protein mediated events
Reactome:R-BTA-111997 CaM pathway
Reactome:R-CEL-74751 Insulin receptor signalling cascade
Reactome:R-CEL-109703 PKB-mediated events
Reactome:R-CEL-111885 Opioid Signalling
Reactome:R-CEL-111996 Ca-dependent events
Reactome:R-DME-162582 Signaling Pathways
Reactome:R-DME-388396 GPCR downstream signalling
Reactome:R-DME-111997 CaM pathway
Reactome:R-DME-111957 Cam-PDE 1 activation
Reactome:R-DME-1489509 DAG and IP3 signaling
Reactome:R-HSA-9006934 Signaling by Receptor Tyrosine Kinases
Reactome:R-HSA-74751 Insulin receptor signalling cascade
Reactome:R-HSA-2404192 Signaling by Type 1 Insulin-like Growth Factor 1 Receptor (IGF1R)
Reactome:R-HSA-372790 Signaling by GPCR
Reactome:R-HSA-418555 G alpha (s) signalling events
Reactome:R-MMU-109703 PKB-mediated events
Reactome:R-MMU-418594 G alpha (i) signalling events
Reactome:R-MMU-112043 PLC beta mediated events
Reactome:R-MMU-111933 Calmodulin induced events
Reactome:R-MMU-111957 Cam-PDE 1 activation
Reactome:R-RNO-112399 IRS-mediated signalling
Reactome:R-RNO-165160 PDE3B signalling
Reactome:R-RNO-2428928 IRS-related events triggered by IGF1R
Reactome:R-RNO-388396 GPCR downstream signalling
Reactome:R-RNO-111885 Opioid Signalling
Reactome:R-RNO-111996 Ca-dependent events
Reactome:R-RNO-1489509 DAG and IP3 signaling
Reactome:R-BTA-388396 GPCR downstream signalling
Reactome:R-BTA-111885 Opioid Signalling
Reactome:R-BTA-111996 Ca-dependent events
Reactome:R-CEL-162582 Signaling Pathways
Reactome:R-CEL-109704 PI3K Cascade
Reactome:R-CEL-2404192 Signaling by Type 1 Insulin-like Growth Factor 1 Receptor (IGF1R)
Reactome:R-CEL-418555 G alpha (s) signalling events
Reactome:R-CEL-112040 G-protein mediated events
Reactome:R-CEL-111933 Calmodulin induced events
Reactome:R-DME-372790 Signaling by GPCR
Reactome:R-DME-418594 G alpha (i) signalling events
Reactome:R-DME-112043 PLC beta mediated events
Reactome:R-DME-9006925 Intracellular signaling by second messengers
Reactome:R-HSA-112399 IRS-mediated signalling
Reactome:R-HSA-165160 PDE3B signalling
Reactome:R-HSA-418594 G alpha (i) signalling events
Reactome:R-HSA-112043 PLC beta mediated events
Reactome:R-HSA-111933 Calmodulin induced events
Reactome:R-HSA-111957 Cam-PDE 1 activation
Reactome:R-MMU-9006934 Signaling by Receptor Tyrosine Kinases
Reactome:R-MMU-74752 Signaling by Insulin receptor
Reactome:R-MMU-109704 PI3K Cascade
Reactome:R-MMU-2404192 Signaling by Type 1 Insulin-like Growth Factor 1 Receptor (IGF1R)
Reactome:R-MMU-372790 Signaling by GPCR
Reactome:R-MMU-418555 G alpha (s) signalling events
Reactome:R-RNO-9006934 Signaling by Receptor Tyrosine Kinases
Reactome:R-RNO-74751 Insulin receptor signalling cascade
Reactome:R-RNO-2428924 IGF1R signaling cascade
Reactome:R-RNO-112040 G-protein mediated events
Reactome:R-RNO-111997 CaM pathway
Reactome:R-RNO-9006925 Intracellular signaling by second messengers
Reactome:R-BTA-9006925 Intracellular signaling by second messengers
Reactome:R-CEL-9006934 Signaling by Receptor Tyrosine Kinases
Reactome:R-CEL-74752 Signaling by Insulin receptor
Reactome:R-CEL-2428924 IGF1R signaling cascade
Reactome:R-CEL-372790 Signaling by GPCR
Reactome:R-CEL-418594 G alpha (i) signalling events
Reactome:R-CEL-112043 PLC beta mediated events
Reactome:R-CEL-1489509 DAG and IP3 signaling
Reactome:R-DME-111885 Opioid Signalling
Reactome:R-DME-111996 Ca-dependent events
Reactome:R-HSA-162582 Signaling Pathways
Reactome:R-HSA-74752 Signaling by Insulin receptor
Reactome:R-HSA-109704 PI3K Cascade
Reactome:R-HSA-2428928 IRS-related events triggered by IGF1R
Reactome:R-HSA-388396 GPCR downstream signalling
Reactome:R-HSA-111885 Opioid Signalling
Reactome:R-HSA-111996 Ca-dependent events
Reactome:R-MMU-74751 Insulin receptor signalling cascade
Reactome:R-MMU-2428924 IGF1R signaling cascade
Reactome:R-MMU-112040 G-protein mediated events
Reactome:R-MMU-111997 CaM pathway
Reactome:R-MMU-9006925 Intracellular signaling by second messengers
Reactome:R-RNO-109703 PKB-mediated events
Reactome:R-RNO-418594 G alpha (i) signalling events
Reactome:R-RNO-112043 PLC beta mediated events
Reactome:R-RNO-111933 Calmodulin induced events
Reactome:R-RNO-111957 Cam-PDE 1 activation
PathBank:SMP0000167 Adenylosuccinate Lyase Deficiency
PathBank:SMP0000220 Xanthine Dehydrogenase Deficiency (Xanthinuria)
PathBank:SMP0000512 Xanthinuria Type I
PathBank:SMP0000536 Mitochondrial DNA Depletion Syndrome-3
PathBank:SMP0000050 Purine Metabolism
PathBank:SMP0000144 Adenosine Deaminase Deficiency
PathBank:SMP0000203 Molybdenum Cofactor Deficiency
PathBank:SMP0000428 Mercaptopurine Action Pathway
PathBank:SMP0000535 Adenine Phosphoribosyltransferase Deficiency (APRT)
PathBank:SMP0000365 Gout or Kelley-Seegmiller Syndrome
PathBank:SMP0000210 Purine Nucleoside Phosphorylase Deficiency
PathBank:SMP0000430 Thioguanine Action Pathway
PathBank:SMP0000513 Xanthinuria Type II
PathBank:SMP0000537 Myoadenylate Deaminase Deficiency
PathBank:SMP0000364 Lesch-Nyhan Syndrome (LNS)
PathBank:SMP0000168 AICA-Ribosiduria
PathBank:SMP0000427 Azathioprine Action Pathway