EC: 3.1.3.57

inositol-1,4-bisphosphate 1-phosphatase (1D-myo-inositol-1,4-bisphosphate 1-phosphohydrolase)

enzyme lambda metabolic reaction experiment
- [Mg2+]

1D_myo_Inositol_1,4_bisphosphate + H2O -> myo_Inositol_4_phosphate + Phosphate		 (( (Vmax * S) ) / (Km + S)) buffer: 120 mM KCl, 10 mM NaCl, 1 mM EGTA, 0.33 mM CaCl2 (0.1 µM free Ca2+), 20 mM Hepes, 0.2 mg of saponin/ml
PH: 7.2
Temperature: 37
uniprot:P21327 [Li+]

1D_myo_Inositol_1,4_bisphosphate + H2O -> Phosphate + myo_Inositol_4_phosphate		 (( (Vmax * S) ) / (Km + (S * (1 + (I / Ki))))) buffer: 50 mM HEPES, 2 mM MgCl2, 0.5 mM EGTA, 100 mM KCl
PH: 7.5
Temperature: 37
uniprot:P21327 [Li+]

1D_myo_Inositol_1,3,4_trisphosphate + H2O -> D_myo_Inositol_3,4_bisphosphate + Phosphate		 (( (Vmax * S) ) / (Km + (S * (1 + (I / Ki))))) buffer: 50 mM HEPES, 100 mM KCl, 2 mM MgCl2, 0.5 mM EGTA
PH: 7.5
Temperature: 37
- [inositol-1,4-bisphosphate 1-phosphatase(Enzyme) wildtype]

H2O + 1D_myo_Inositol_1,4_bisphosphate -> Phosphate + myo_Inositol_4_phosphate		 (( (Vmax * S) ) / (Km + S)) buffer: 1 mM EGTA, 4 mM MgCl2, 10 mM Hepes, 10 mM potassium chloride
PH: 7.5
Temperature: 37
- [inositol-1,4-bisphosphate 1-phosphatase(Enzyme) wildtype]

H2O + 1D_myo_Inositol_1,4_bisphosphate -> Phosphate + myo_Inositol_4_phosphate		 (k * S) buffer: 100 mM KCl, 10 mM NaCl, 10 mM HEPES, 1 mM EGTA, 3 mM MgSO4
PH: 7.2
Temperature: 37
uniprot:P21327 [Mg2+]

H2O + 1D_myo_Inositol_1,4_bisphosphate -> Phosphate + myo_Inositol_4_phosphate		 (( (( (kcat * E) ) * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) buffer: 50 mM HEPES, 100 mM KCl, 0.5 mM EGTA
PH: 7.5
Temperature: 37
uniprot:P21327 [Li+]

H2O + 1D_myo_Inositol_1,4_bisphosphate -> myo_Inositol_4_phosphate + Phosphate		 (( (Vmax * S) ) / (Km + (S * (1 + (I / Ki))))) buffer: 50 mM HEPES, 2 mM MgCl2, 0.5 mM EGTA, 100 mM NaCl
PH: 7.5
Temperature: 37
uniprot:P21327 [inositol-1,4-bisphosphate 1-phosphatase(Enzyme) wildtype]

1D_myo_Inositol_1,4_bisphosphate + H2O -> Phosphate + myo_Inositol_4_phosphate		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM HEPES, 100 mM KCl, 2 mM MgCl2, 0.5 mM EGTA
PH: 7.5
Temperature: 37
uniprot:P21327 [inositol-1,4-bisphosphate 1-phosphatase(Enzyme) wildtype]

H2O + 1D_myo_Inositol_1,3,4_trisphosphate -> D_myo_Inositol_3,4_bisphosphate + Phosphate		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM HEPES, 100 mM KCl, 2 mM MgCl2, 0.5 mM EGTA
PH: 7.5
Temperature: 37

Pathways

pathway id name
BioCyc:META_PWY-6358 superpathway of D-myo-inositol (1,4,5)-trisphosphate metabolism
BioCyc:ARA_PWY-6363 D-myo-inositol (1,4,5)-trisphosphate degradation
BioCyc:META_PWY-6363 D-myo-inositol (1,4,5)-trisphosphate degradation
BioCyc:MOUSE_PWY-6358 superpathway of D-myo-inositol (1,4,5)-trisphosphate metabolism
BioCyc:HUMAN_PWY-6363 D-myo-inositol (1,4,5)-trisphosphate degradation
BioCyc:HUMAN_PWY-6358 superpathway of D-myo-inositol (1,4,5)-trisphosphate metabolism
BioCyc:MOUSE_PWY-6363 D-myo-inositol (1,4,5)-trisphosphate degradation
PlantCyc:ARA_PWY-6363 D-myo-inositol (1,4,5)-trisphosphate degradation
Reactome:R-BTA-1430728 Metabolism
Reactome:R-DDI-1483249 Inositol phosphate metabolism
Reactome:R-DDI-211859 Biological oxidations
Reactome:R-DDI-156584 Cytosolic sulfonation of small molecules
Reactome:R-HSA-211859 Biological oxidations
Reactome:R-MMU-1430728 Metabolism
Reactome:R-MMU-211859 Biological oxidations
Reactome:R-BTA-1855183 Synthesis of IP2, IP, and Ins in the cytosol
Reactome:R-BTA-211859 Biological oxidations
Reactome:R-HSA-156580 Phase II - Conjugation of compounds
Reactome:R-MMU-1855183 Synthesis of IP2, IP, and Ins in the cytosol
Reactome:R-MMU-156580 Phase II - Conjugation of compounds
Reactome:R-RNO-211859 Biological oxidations
Reactome:R-BTA-156584 Cytosolic sulfonation of small molecules
Reactome:R-DDI-1855183 Synthesis of IP2, IP, and Ins in the cytosol
Reactome:R-DDI-156580 Phase II - Conjugation of compounds
Reactome:R-RNO-1430728 Metabolism
Reactome:R-RNO-156584 Cytosolic sulfonation of small molecules
Reactome:R-BTA-1483249 Inositol phosphate metabolism
Reactome:R-BTA-156580 Phase II - Conjugation of compounds
Reactome:R-DDI-1430728 Metabolism
Reactome:R-HSA-1430728 Metabolism
Reactome:R-HSA-1483249 Inositol phosphate metabolism
Reactome:R-HSA-1855183 Synthesis of IP2, IP, and Ins in the cytosol
Reactome:R-HSA-156584 Cytosolic sulfonation of small molecules
Reactome:R-MMU-1483249 Inositol phosphate metabolism
Reactome:R-MMU-156584 Cytosolic sulfonation of small molecules
Reactome:R-RNO-156580 Phase II - Conjugation of compounds
PathBank:SMP0000041 Sulfate/Sulfite Metabolism
PathBank:SMP0000532 Sulfite Oxidase Deficiency
PathBank:SMP0000011 Inositol Metabolism
PathBank:SMP0000462 Inositol Phosphate Metabolism
PathBank:SMP0087349 Inositol Phosphate Metabolism
PathBank:SMP0120816 Sulfite Oxidase Deficiency
PathBank:SMP0087311 Sulfate/Sulfite Metabolism
PathBank:SMP0087335 Inositol Metabolism