EC: 2.3.1.21

carnitine O-palmitoyltransferase (palmitoyl-CoA:L-carnitine O-palmitoyltransferase)

enzyme lambda metabolic reaction experiment
uniprot:P32198 [carnitine O-palmitoyltransferase(Enzyme) mutant delta19-30]

Palmitoyl_CoA + L_Carnitine -> Coenzyme_A + L_Palmitoylcarnitine		 (( (Vmax * A) ) / (Km + A)) buffer: 20 mM Hepes, 150 mM KCl, 1 mM EGTA, 5 mM MgATP, 1% (w/v) dialysed defatted BSA, 1 mM dithiothreitol, 4 µg/ml rotenone, 2 µg/ml antimycin A
PH: 7
Temperature: 30
uniprot:P32198 [carnitine O-palmitoyltransferase(Enzyme) mutant E3A/delta19-46]

Palmitoyl_CoA + L_Carnitine -> Coenzyme_A + L_Palmitoylcarnitine		 (( (Vmax * A) ) / (Km + A)) buffer: 20 mM Hepes, 150 mM KCl, 1 mM EGTA, 5 mM MgATP, 1% (w/v) dialysed defatted BSA, 1 mM dithiothreitol, 4 µg/ml rotenone, 2 µg/ml antimycin A
PH: 7
Temperature: 30
uniprot:P32198 [carnitine O-palmitoyltransferase(Enzyme) mutant delta19-46]

L_Carnitine + Palmitoyl_CoA -> L_Palmitoylcarnitine + Coenzyme_A		 (( (Vmax * A) ) / (Km + A)) buffer: 20 mM Hepes, 150 mM KCl, 1 mM EGTA, 5 mM MgATP, 1% (w/v) dialysed defatted BSA, 1 mM dithiothreitol, 4 µg/ml rotenone, 2 µg/ml antimycin A
PH: 7
Temperature: 30
uniprot:P32198 [carnitine O-palmitoyltransferase(Enzyme) mutant delta3-18]

L_Carnitine + Palmitoyl_CoA -> Coenzyme_A + L_Palmitoylcarnitine		 (( (Vmax * A) ) / (Km + A)) buffer: 20 mM Hepes, 150 mM KCl, 1 mM EGTA, 5 mM MgATP, 1% (w/v) dialysed defatted BSA, 1 mM dithiothreitol, 4 µg/ml rotenone, 2 µg/ml antimycin A
PH: 7
Temperature: 30
uniprot:P32198 [carnitine O-palmitoyltransferase(Enzyme) mutant delta1-82]

L_Carnitine + Palmitoyl_CoA -> Coenzyme_A + L_Palmitoylcarnitine		 (( (Vmax * A) ) / (Km + A)) buffer: 20 mM Hepes, 150 mM KCl, 1 mM EGTA, 5 mM MgATP, 1% (w/v) dialysed defatted BSA, 1 mM dithiothreitol, 4 µg/ml rotenone, 2 µg/ml antimycin A
PH: 7
Temperature: 30
uniprot:P32198 [carnitine O-palmitoyltransferase(Enzyme) mutant E3A]

L_Carnitine + Palmitoyl_CoA -> Coenzyme_A + L_Palmitoylcarnitine		 (( (Vmax * A) ) / (Km + A)) buffer: 20 mM Hepes, 150 mM KCl, 1 mM EGTA, 5 mM MgATP, 1% (w/v) dialysed defatted BSA, 1 mM dithiothreitol, 4 µg/ml rotenone, 2 µg/ml antimycin A
PH: 7
Temperature: 30
uniprot:P32198 [carnitine O-palmitoyltransferase(Enzyme) mutant E3A/delta19-30]

L_Carnitine + Palmitoyl_CoA -> Coenzyme_A + L_Palmitoylcarnitine		 (( (Vmax * A) ) / (Km + A)) buffer: 20 mM Hepes, 150 mM KCl, 1 mM EGTA, 5 mM MgATP, 1% (w/v) dialysed defatted BSA, 1 mM dithiothreitol, 4 µg/ml rotenone, 2 µg/ml antimycin A
PH: 7
Temperature: 30
uniprot:Q63704 [carnitine O-palmitoyltransferase(Enzyme) mutant delta3-18]

Palmitoyl_CoA + L_Carnitine -> L_Palmitoylcarnitine + Coenzyme_A		 (( (Vmax * A) ) / (Km + A)) buffer: 20 mM Hepes, 150 mM KCl, 1 mM EGTA, 5 mM MgATP, 1% (w/v) dialysed defatted BSA, 1 mM dithiothreitol, 4 µg/ml rotenone, 2 µg/ml antimycin A
PH: 7
Temperature: 30
uniprot:Q63704 [carnitine O-palmitoyltransferase(Enzyme) mutant delta1-80]

L_Carnitine + Palmitoyl_CoA -> Coenzyme_A + L_Palmitoylcarnitine		 (( (Vmax * A) ) / (Km + A)) buffer: 20 mM Hepes, 150 mM KCl, 1 mM EGTA, 5 mM MgATP, 1% (w/v) dialysed defatted BSA, 1 mM dithiothreitol, 4 µg/ml rotenone, 2 µg/ml antimycin A
PH: 7
Temperature: 30
uniprot:Q63704 [carnitine O-palmitoyltransferase(Enzyme) mutant delta3-18]

L_Carnitine + Palmitoyl_CoA -> Coenzyme_A + L_Palmitoylcarnitine		 (( (Vmax * B) ) / (K_app + B)) buffer: 20 mM Hepes, 150 mM KCl, 1 mM EGTA, 5 mM MgATP, 1% (w/v) dialysed defatted BSA, 1 mM dithiothreitol, 4 µg/ml rotenone, 2 µg/ml antimycin A
PH: 7
Temperature: 30
uniprot:P32198 [carnitine O-palmitoyltransferase(Enzyme) mutant delta19-30]

Palmitoyl_CoA + L_Carnitine -> L_Palmitoylcarnitine + Coenzyme_A		 (( (Vmax * B) ) / (K_app + B)) buffer: 20 mM Hepes, 150 mM KCl, 1 mM EGTA, 5 mM MgATP, 1% (w/v) dialysed defatted BSA, 1 mM dithiothreitol, 4 µg/ml rotenone, 2 µg/ml antimycin A
PH: 7
Temperature: 30
uniprot:P32198 [carnitine O-palmitoyltransferase(Enzyme) mutant delta3-18]

L_Carnitine + Palmitoyl_CoA -> L_Palmitoylcarnitine + Coenzyme_A		 (( (Vmax * B) ) / (K_app + B)) buffer: 20 mM Hepes, 150 mM KCl, 1 mM EGTA, 5 mM MgATP, 1% (w/v) dialysed defatted BSA, 1 mM dithiothreitol, 4 µg/ml rotenone, 2 µg/ml antimycin A
PH: 7
Temperature: 30
uniprot:P32198 [carnitine O-palmitoyltransferase(Enzyme) mutant E3A/delta19-30]

L_Carnitine + Palmitoyl_CoA -> L_Palmitoylcarnitine + Coenzyme_A		 (( (Vmax * B) ) / (K_app + B)) buffer: 20 mM Hepes, 150 mM KCl, 1 mM EGTA, 5 mM MgATP, 1% (w/v) dialysed defatted BSA, 1 mM dithiothreitol, 4 µg/ml rotenone, 2 µg/ml antimycin A
PH: 7
Temperature: 30
uniprot:P32198 [carnitine O-palmitoyltransferase(Enzyme) mutant E3A/delta19-46]

L_Carnitine + Palmitoyl_CoA -> L_Palmitoylcarnitine + Coenzyme_A		 (( (Vmax * B) ) / (K_app + B)) buffer: 20 mM Hepes, 150 mM KCl, 1 mM EGTA, 5 mM MgATP, 1% (w/v) dialysed defatted BSA, 1 mM dithiothreitol, 4 µg/ml rotenone, 2 µg/ml antimycin A
PH: 7
Temperature: 30
uniprot:P32198 [carnitine O-palmitoyltransferase(Enzyme) mutant delta19-46]

Palmitoyl_CoA + L_Carnitine -> Coenzyme_A + L_Palmitoylcarnitine		 (( (Vmax * B) ) / (K_app + B)) buffer: 20 mM Hepes, 150 mM KCl, 1 mM EGTA, 5 mM MgATP, 1% (w/v) dialysed defatted BSA, 1 mM dithiothreitol, 4 µg/ml rotenone, 2 µg/ml antimycin A
PH: 7
Temperature: 30
uniprot:P32198 [carnitine O-palmitoyltransferase(Enzyme) mutant E3A]

L_Carnitine + Palmitoyl_CoA -> Coenzyme_A + L_Palmitoylcarnitine		 (( (Vmax * B) ) / (K_app + B)) buffer: 20 mM Hepes, 150 mM KCl, 1 mM EGTA, 5 mM MgATP, 1% (w/v) dialysed defatted BSA, 1 mM dithiothreitol, 4 µg/ml rotenone, 2 µg/ml antimycin A
PH: 7
Temperature: 30
uniprot:P32198 [carnitine O-palmitoyltransferase(Enzyme) mutant delta1-82]

L_Carnitine + Palmitoyl_CoA -> L_Palmitoylcarnitine + Coenzyme_A		 (( (Vmax * B) ) / (K_app + B)) buffer: 20 mM Hepes, 150 mM KCl, 1 mM EGTA, 5 mM MgATP, 1% (w/v) dialysed defatted BSA, 1 mM dithiothreitol, 4 µg/ml rotenone, 2 µg/ml antimycin A
PH: 7
Temperature: 30
uniprot:Q63704 [carnitine O-palmitoyltransferase(Enzyme) mutant delta1-80]

L_Carnitine + Palmitoyl_CoA -> Coenzyme_A + L_Palmitoylcarnitine		 (( (Vmax * B) ) / (K_app + B)) buffer: 20 mM Hepes, 150 mM KCl, 1 mM EGTA, 5 mM MgATP, 1% (w/v) dialysed defatted BSA, 1 mM dithiothreitol, 4 µg/ml rotenone, 2 µg/ml antimycin A
PH: 7
Temperature: 30
uniprot:Q63704 [carnitine O-palmitoyltransferase(Enzyme) wildtype isoenzyme CPTI-M]

Palmitoyl_CoA + L_Carnitine -> Coenzyme_A + L_Palmitoylcarnitine		 (( (Vmax * B) ) / (Km + B)) buffer: 20 mM Hepes, 150 mM KCl, 1 mM EGTA, 5 mM MgATP, 1 % BSA, 1 mM dithiothreitol, 4 µg/ml rotenone, 2 µg/ml antimycin A
PH: 7
Temperature: 30
uniprot:P32198 [carnitine O-palmitoyltransferase(Enzyme) wildtype isoenzyme CPTI-L]

L_Carnitine + Palmitoyl_CoA -> L_Palmitoylcarnitine + Coenzyme_A		 (( (Vmax * B) ) / (Km + B)) buffer: 20 mM Hepes, 150 mM KCl, 1 mM EGTA, 5 mM MgATP, 1 % BSA, 1 mM dithiothreitol, 4 µg/ml rotenone, 2 µg/ml antimycin A
PH: 7
Temperature: 30
- [carnitine O-palmitoyltransferase(Enzyme) wildtype chimeric protein CPTI-L (1-82)/CPTI-M (83-772)]

L_Carnitine + Palmitoyl_CoA -> L_Palmitoylcarnitine + Coenzyme_A		 (( (Vmax * B) ) / (Km + B)) buffer: 20 mM Hepes, 150 mM KCl, 1 mM EGTA, 5 mM MgATP, 1 % BSA, 1 mM dithiothreitol, 4 µg/ml rotenone, 2 µg/ml antimycin A
PH: 7
Temperature: 30
- [carnitine O-palmitoyltransferase(Enzyme) wildtype chimeric protein CPTI-M (1-82)/CPTI-L (83-772)]

Palmitoyl_CoA + L_Carnitine -> L_Palmitoylcarnitine + Coenzyme_A		 (( (Vmax * B) ) / (Km + B)) buffer: 20 mM Hepes, 150 mM KCl, 1 mM EGTA, 5 mM MgATP, 1 % BSA, 1 mM dithiothreitol, 4 µg/ml rotenone, 2 µg/ml antimycin A
PH: 7
Temperature: 30
- [carnitine O-palmitoyltransferase(Enzyme) wildtype chimeric protein CPTI-M (1-126)/CPTI-L (127-772)]

Palmitoyl_CoA + L_Carnitine -> L_Palmitoylcarnitine + Coenzyme_A		 (( (Vmax * B) ) / (Km + B)) buffer: 20 mM Hepes, 150 mM KCl, 1 mM EGTA, 5 mM MgATP, 1 % BSA, 1 mM dithiothreitol, 4 µg/ml rotenone, 2 µg/ml antimycin A
PH: 7
Temperature: 30
- [carnitine O-palmitoyltransferase(Enzyme) wildtype chimeric protein CPTI-L (1-82)/CPTI-M (83-126)/CPTI-L (127-772)]

L_Carnitine + Palmitoyl_CoA -> Coenzyme_A + L_Palmitoylcarnitine		 (( (Vmax * B) ) / (Km + B)) buffer: 20 mM Hepes, 150 mM KCl, 1 mM EGTA, 5 mM MgATP, 1 % BSA, 1 mM dithiothreitol, 4 µg/ml rotenone, 2 µg/ml antimycin A
PH: 7
Temperature: 30
uniprot:Q63704 [carnitine O-palmitoyltransferase(Enzyme) wildtype isoenzyme CPTI-M]

Palmitoyl_CoA + L_Carnitine -> Coenzyme_A + L_Palmitoylcarnitine		 (( (Vmax * A) ) / (Km + A)) buffer: 20 mM Hepes, 150 mM KCl, 1 mM EGTA, 5 mM MgATP, 1 % BSA, 1 mM dithiothreitol, 4 µg/ml rotenone, 2 µg/ml antimycin A
PH: 7
Temperature: 30
uniprot:P32198 [carnitine O-palmitoyltransferase(Enzyme) wildtype isoenzyme CPTI-L]

Palmitoyl_CoA + L_Carnitine -> Coenzyme_A + L_Palmitoylcarnitine		 (( (Vmax * A) ) / (Km + A)) buffer: 20 mM Hepes, 150 mM KCl, 1 mM EGTA, 5 mM MgATP, 1 % BSA, 1 mM dithiothreitol, 4 µg/ml rotenone, 2 µg/ml antimycin A
PH: 7
Temperature: 30
- [carnitine O-palmitoyltransferase(Enzyme) wildtype chimeric protein CPTI-M (1-82)/CPTI-L (83-126)/CPTI-M (127-772)]

L_Carnitine + Palmitoyl_CoA -> L_Palmitoylcarnitine + Coenzyme_A		 (( (Vmax * B) ) / (Km + B)) buffer: 20 mM Hepes, 150 mM KCl, 1 mM EGTA, 5 mM MgATP, 1 % BSA, 1 mM dithiothreitol, 4 µg/ml rotenone, 2 µg/ml antimycin A
PH: 7
Temperature: 30
- [carnitine O-palmitoyltransferase(Enzyme) wildtype chimeric protein CPTI-L (1-126)/CPTI-M (127-772)]

Palmitoyl_CoA + L_Carnitine -> Coenzyme_A + L_Palmitoylcarnitine		 (( (Vmax * B) ) / (Km + B)) buffer: 20 mM Hepes, 150 mM KCl, 1 mM EGTA, 5 mM MgATP, 1 % BSA, 1 mM dithiothreitol, 4 µg/ml rotenone, 2 µg/ml antimycin A
PH: 7
Temperature: 30
- [carnitine O-palmitoyltransferase(Enzyme) wildtype chimeric protein CPTI-L (1-126)/CPTI-M (127-772)]

L_Carnitine + Palmitoyl_CoA -> L_Palmitoylcarnitine + Coenzyme_A		 (( (Vmax * A) ) / (Km + A)) buffer: 20 mM Hepes, 150 mM KCl, 1 mM EGTA, 5 mM MgATP, 1 % BSA, 1 mM dithiothreitol, 4 µg/ml rotenone, 2 µg/ml antimycin A
PH: 7
Temperature: 30
- [carnitine O-palmitoyltransferase(Enzyme) wildtype chimeric protein CPTI-L (1-82)/CPTI-M (83-126)/CPTI-L (127-772)]

Palmitoyl_CoA + L_Carnitine -> L_Palmitoylcarnitine + Coenzyme_A		 (( (Vmax * A) ) / (Km + A)) buffer: 20 mM Hepes, 150 mM KCl, 1 mM EGTA, 5 mM MgATP, 1 % BSA, 1 mM dithiothreitol, 4 µg/ml rotenone, 2 µg/ml antimycin A
PH: 7
Temperature: 30
- [carnitine O-palmitoyltransferase(Enzyme) wildtype chimeric protein CPTI-M (1-82)/CPTI-L (83-772)]

L_Carnitine + Palmitoyl_CoA -> Coenzyme_A + L_Palmitoylcarnitine		 (( (Vmax * A) ) / (Km + A)) buffer: 20 mM Hepes, 150 mM KCl, 1 mM EGTA, 5 mM MgATP, 1 % BSA, 1 mM dithiothreitol, 4 µg/ml rotenone, 2 µg/ml antimycin A
PH: 7
Temperature: 30
- [carnitine O-palmitoyltransferase(Enzyme) wildtype chimeric protein CPTI-M (1-126)/CPTI-L (127-772)]

Palmitoyl_CoA + L_Carnitine -> L_Palmitoylcarnitine + Coenzyme_A		 (( (Vmax * A) ) / (Km + A)) buffer: 20 mM Hepes, 150 mM KCl, 1 mM EGTA, 5 mM MgATP, 1 % BSA, 1 mM dithiothreitol, 4 µg/ml rotenone, 2 µg/ml antimycin A
PH: 7
Temperature: 30
- [carnitine O-palmitoyltransferase(Enzyme) wildtype chimeric protein CPTI-L (1-82)/CPTI-M (83-772)]

Palmitoyl_CoA + L_Carnitine -> L_Palmitoylcarnitine + Coenzyme_A		 (( (Vmax * A) ) / (Km + A)) buffer: 20 mM Hepes, 150 mM KCl, 1 mM EGTA, 5 mM MgATP, 1 % BSA, 1 mM dithiothreitol, 4 µg/ml rotenone, 2 µg/ml antimycin A
PH: 7
Temperature: 30
- [carnitine O-palmitoyltransferase(Enzyme) wildtype chimeric protein CPTI-M (1-82)/CPTI-L (83-126)/CPTI-M (127-772)]

Palmitoyl_CoA + L_Carnitine -> Coenzyme_A + L_Palmitoylcarnitine		 (( (Vmax * A) ) / (Km + A)) buffer: 20 mM Hepes, 150 mM KCl, 1 mM EGTA, 5 mM MgATP, 1 % BSA, 1 mM dithiothreitol, 4 µg/ml rotenone, 2 µg/ml antimycin A
PH: 7
Temperature: 30
uniprot:P32198 [Bovine serum albumin]

Palmitoyl_CoA + L_Carnitine -> L_Palmitoylcarnitine + Coenzyme_A		 (( (Vmax * B) ) / (Km + B)) buffer: 20 mM HEPES, 40 - 75 mM KCl
PH: 7
Temperature: 30
uniprot:P32198 [Bovine serum albumin]

Palmitoyl_CoA + L_Carnitine -> Coenzyme_A + L_Palmitoylcarnitine		 (( (Vmax * B) ) / (Km + B)) buffer: 20 mM HEPES, 40 - 75 mM KCl
PH: 7
Temperature: 30
uniprot:P32198 [carnitine O-palmitoyltransferase(Enzyme) wildtype isoenzyme CPTI-L]

Palmitoyl_CoA + L_Carnitine -> Coenzyme_A + L_Palmitoylcarnitine		 (( (Vmax * A) ) / (Km + A)) buffer: 20 mM HEPES, 1 % BSA, 40 - 75 mM KCl
PH: 7
Temperature: 30
uniprot:P32198 [carnitine O-palmitoyltransferase(Enzyme) mutant isoenzyme CPTI-L E590Q]

L_Carnitine + Palmitoyl_CoA -> Coenzyme_A + L_Palmitoylcarnitine		 (( (Vmax * A) ) / (Km + A)) buffer: 20 mM HEPES, 1 % BSA, 40 - 75 mM KCl
PH: 7
Temperature: 30
uniprot:P32198 [carnitine O-palmitoyltransferase(Enzyme) mutant isoenzyme CPTI-L E590A]

L_Carnitine + Palmitoyl_CoA -> L_Palmitoylcarnitine + Coenzyme_A		 (( (Vmax * A) ) / (Km + A)) buffer: 20 mM HEPES, 1 % BSA, 40 - 75 mM KCl
PH: 7
Temperature: 30
uniprot:P32198 [Bovine serum albumin]

L_Carnitine + Palmitoyl_CoA -> Coenzyme_A + L_Palmitoylcarnitine		 (( (Vmax * B) ) / (Km + B)) buffer: 20 mM HEPES, 40 - 75 mM KCl
PH: 7
Temperature: 30
uniprot:P32198 [Bovine serum albumin]

L_Carnitine + Palmitoyl_CoA -> Coenzyme_A + L_Palmitoylcarnitine		 (( (Vmax * B) ) / (Km + B)) buffer: 20 mM HEPES, 40 - 75 mM KCl
PH: 7
Temperature: 30
uniprot:P32198 [Bovine serum albumin]

Palmitoyl_CoA + L_Carnitine -> Coenzyme_A + L_Palmitoylcarnitine		 (( (Vmax * B) ) / (Km + B)) buffer: 20 mM HEPES, 40 - 75 mM KCl
PH: 7
Temperature: 30
uniprot:P32198 [carnitine O-palmitoyltransferase(Enzyme) mutant isoenzyme CPTI-L E603Q]

Palmitoyl_CoA + L_Carnitine -> Coenzyme_A + L_Palmitoylcarnitine		 (( (Vmax * A) ) / (Km + A)) buffer: 20 mM HEPES, 1 % BSA, 40 - 75 mM KCl
PH: 7
Temperature: 30
uniprot:P32198 [carnitine O-palmitoyltransferase(Enzyme) wildtype isoenzyme CPTI-L]

L_Carnitine + Palmitoyl_CoA -> Coenzyme_A + L_Palmitoylcarnitine		 (( (Vmax * A) ) / (Km + A)) buffer: 20 mM HEPES, 1 % BSA, 40 - 75 mM KCl
PH: 7
Temperature: 30
uniprot:P32198 [Bovine serum albumin]

Palmitoyl_CoA + L_Carnitine -> L_Palmitoylcarnitine + Coenzyme_A		 (( (Vmax * B) ) / (Km + B)) buffer: 20 mM HEPES, 40 - 75 mM KCl
PH: 7
Temperature: 30
uniprot:P32198 [Bovine serum albumin]

Palmitoyl_CoA + L_Carnitine -> L_Palmitoylcarnitine + Coenzyme_A		 (( (Vmax * B) ) / (Km + B)) buffer: 20 mM HEPES, 40 - 75 mM KCl
PH: 7
Temperature: 30
uniprot:P32198 [Bovine serum albumin]

L_Carnitine + Palmitoyl_CoA -> L_Palmitoylcarnitine + Coenzyme_A		 (( (Vmax * B) ) / (Km + B)) buffer: 20 mM HEPES, 40 - 75 mM KCl
PH: 7
Temperature: 30
uniprot:P32198 [Bovine serum albumin]

L_Carnitine + Palmitoyl_CoA -> L_Palmitoylcarnitine + Coenzyme_A		 (( (Vmax * B) ) / (Km + B)) buffer: 20 mM HEPES, 40 - 75 mM KCl
PH: 7
Temperature: 30
uniprot:P32198 [carnitine O-palmitoyltransferase(Enzyme) mutant isoenzyme CPTI-L E603D]

L_Carnitine + Palmitoyl_CoA -> Coenzyme_A + L_Palmitoylcarnitine		 (( (Vmax * A) ) / (Km + A)) buffer: 20 mM HEPES, 1 % BSA, 40 - 75 mM KCl
PH: 7
Temperature: 30
uniprot:P32198 [carnitine O-palmitoyltransferase(Enzyme) mutant isoenzyme CPTI-L delta767-773]

Palmitoyl_CoA + L_Carnitine -> Coenzyme_A + L_Palmitoylcarnitine		 (( (Vmax * A) ) / (Km + A)) buffer: 20 mM HEPES, 1 % BSA, 40 - 75 mM KCl
PH: 7
Temperature: 30
uniprot:P32198 [carnitine O-palmitoyltransferase(Enzyme) wildtype isoenzyme CPTI-L]

Palmitoyl_CoA + L_Carnitine -> Coenzyme_A + L_Palmitoylcarnitine		 (( (Vmax * A) ) / (Km + A)) buffer: 20 mM HEPES, 1 % BSA, 40 - 75 mM KCl
PH: 7
Temperature: 30
uniprot:P32198 [carnitine O-palmitoyltransferase(Enzyme) mutant isoenzyme CPTI-L delta772-773]

L_Carnitine + Palmitoyl_CoA -> L_Palmitoylcarnitine + Coenzyme_A		 (( (Vmax * A) ) / (Km + A)) buffer: 20 mM HEPES, 1 % BSA, 40 - 75 mM KCl
PH: 7
Temperature: 30
uniprot:P32198 [carnitine O-palmitoyltransferase(Enzyme) wildtype isoenzyme CPTI-L]

L_Carnitine + Palmitoyl_CoA -> L_Palmitoylcarnitine + Coenzyme_A		 (( (Vmax * A) ) / (Km + A)) buffer: 20 mM HEPES, 1 % BSA, 40 - 75 mM KCl
PH: 7
Temperature: 30
uniprot:P32198 [Bovine serum albumin]

L_Carnitine + Palmitoyl_CoA -> Coenzyme_A + L_Palmitoylcarnitine		 (( (Vmax * B) ) / (Km + B)) buffer: 20 mM HEPES, 40 - 75 mM KCl
PH: 7
Temperature: 30
uniprot:P32198 [Bovine serum albumin]

Palmitoyl_CoA + L_Carnitine -> L_Palmitoylcarnitine + Coenzyme_A		 (( (Vmax * B) ) / (Km + B)) buffer: 20 mM HEPES, 40 - 75 mM KCl
PH: 7
Temperature: 30
uniprot:P32198 [carnitine O-palmitoyltransferase(Enzyme) mutant isoenzyme CPTI-L E3A]

L_Carnitine + Palmitoyl_CoA -> Coenzyme_A + L_Palmitoylcarnitine		 (( (Vmax * A) ) / (Km + A)) buffer: 20 mM HEPES, 1 % BSA, 40 - 75 mM KCl
PH: 7
Temperature: 30
uniprot:Q92523 [carnitine O-palmitoyltransferase(Enzyme) wildtype]

Palmitoyl_CoA + L_Carnitine -> Coenzyme_A + L_Palmitoylcarnitine		 (( (Vmax * B) ) / (Km + B)) buffer: 20 mM Hepes, 1% fatty acid-free albumin, 40-75 mM KCl
PH: 7
Temperature: 30
uniprot:P32198 [carnitine O-palmitoyltransferase(Enzyme) mutant isoenzyme Cpt1a R388A]

Palmitoyl_CoA + L_Carnitine -> Coenzyme_A + L_Palmitoylcarnitine		 (( (Vmax * C) ) / (Km + C)) buffer: 20 mM Hepes, 40-75 mM KCl, 1-2 % albumin
PH: 7
Temperature: 30
uniprot:P32198 [carnitine O-palmitoyltransferase(Enzyme) mutant isoenzyme Cpt1a W452A]

L_Carnitine + Palmitoyl_CoA -> Coenzyme_A + L_Palmitoylcarnitine		 (( (Vmax * C) ) / (Km + C)) buffer: 20 mM Hepes, 40-75 mM KCl, 1-2 % albumin
PH: 7
Temperature: 30
uniprot:P32198 [carnitine O-palmitoyltransferase(Enzyme) mutant isoenzyme Cpt1a R451A]

Palmitoyl_CoA + L_Carnitine -> Coenzyme_A + L_Palmitoylcarnitine		 (( (Vmax * C) ) / (Km + C)) buffer: 20 mM Hepes, 40-75 mM KCl, 1-2 % albumin
PH: 7
Temperature: 30
uniprot:P32198 [carnitine O-palmitoyltransferase(Enzyme) wildtype isoenzyme Cpt1a]

L_Carnitine + Palmitoyl_CoA -> L_Palmitoylcarnitine + Coenzyme_A		 (( (Vmax * C) ) / (Km + C)) buffer: 20 mM Hepes, 40-75 mM KCl, 1-2 % albumin
PH: 7
Temperature: 30
uniprot:P32198 [carnitine O-palmitoyltransferase(Enzyme) mutant isoenzyme Cpt1a R606A]

Palmitoyl_CoA + L_Carnitine -> Coenzyme_A + L_Palmitoylcarnitine		 (( (Vmax * C) ) / (Km + C)) buffer: 20 mM Hepes, 40-75 mM KCl, 1-2 % albumin
PH: 7
Temperature: 30
uniprot:P32198 [carnitine O-palmitoyltransferase(Enzyme) mutant isoenzyme Cpt1a W391A]

L_Carnitine + Palmitoyl_CoA -> L_Palmitoylcarnitine + Coenzyme_A		 (( (Vmax * C) ) / (Km + C)) buffer: 20 mM Hepes, 40-75 mM KCl, 1-2 % albumin
PH: 7
Temperature: 30
uniprot:P32198 [carnitine O-palmitoyltransferase(Enzyme) wildtype isoenzyme Cpt1a]

L_Carnitine + Palmitoyl_CoA -> L_Palmitoylcarnitine + Coenzyme_A		 (( (Vmax * C) ) / (Km + C)) buffer: 20 mM Hepes, 40-75 mM KCl
PH: 7
Temperature: 30
uniprot:P32198 [carnitine O-palmitoyltransferase(Enzyme) mutant isoenzyme Cpt1a R606A]

L_Carnitine + Palmitoyl_CoA -> L_Palmitoylcarnitine + Coenzyme_A		 (( (Vmax * C) ) / (Km + C)) buffer: 20 mM Hepes, 40-75 mM KCl
PH: 7
Temperature: 30
uniprot:P32198 [carnitine O-palmitoyltransferase(Enzyme) mutant isoenzyme Cpt1a R388A]

Palmitoyl_CoA + L_Carnitine -> Coenzyme_A + L_Palmitoylcarnitine		 (( (Vmax * C) ) / (Km + C)) buffer: 20 mM Hepes, 40-75 mM KCl
PH: 7
Temperature: 30
uniprot:P32198 [carnitine O-palmitoyltransferase(Enzyme) mutant isoenzyme Cpt1a W391A]

L_Carnitine + Palmitoyl_CoA -> L_Palmitoylcarnitine + Coenzyme_A		 (( (Vmax * C) ) / (Km + C)) buffer: 20 mM Hepes, 40-75 mM KCl
PH: 7
Temperature: 30
uniprot:P32198 [carnitine O-palmitoyltransferase(Enzyme) mutant isoenzyme Cpt1a R451A]

L_Carnitine + Palmitoyl_CoA -> L_Palmitoylcarnitine + Coenzyme_A		 (( (Vmax * C) ) / (Km + C)) buffer: 20 mM Hepes, 40-75 mM KCl
PH: 7
Temperature: 30
uniprot:P32198 [carnitine O-palmitoyltransferase(Enzyme) mutant isoenzyme Cpt1a W452A]

L_Carnitine + Palmitoyl_CoA -> L_Palmitoylcarnitine + Coenzyme_A		 (( (Vmax * C) ) / (Km + C)) buffer: 20 mM Hepes, 40-75 mM KCl
PH: 7
Temperature: 30
uniprot:Q63704 [carnitine O-palmitoyltransferase(Enzyme) wildtype]

L_Carnitine + Palmitoyl_CoA -> Coenzyme_A + L_Palmitoylcarnitine		 (( (Vmax * A) ) / (Km + A)) buffer: 75 mM KCl, 80 mM Mannitol, 1 mM EGTA, 1 mM DTT
PH: 7
Temperature: 37
uniprot:Q63704 [carnitine O-palmitoyltransferase(Enzyme) wildtype]

Palmitoyl_CoA + L_Carnitine -> L_Palmitoylcarnitine + Coenzyme_A		 (( (Vmax * A) ) / (Km + A)) buffer: 75 mM KCl, 80 mM Mannitol, 1 mM EGTA, 1 mM DTT
PH: 7
Temperature: 37
uniprot:Q63704 [carnitine O-palmitoyltransferase(Enzyme) wildtype]

Palmitoyl_CoA + L_Carnitine -> L_Palmitoylcarnitine + Coenzyme_A		 (( (Vmax * B) ) / (Km + B)) buffer: 75 mM KCl, 80 mM Mannitol, 1 mM EGTA, 1 mM DTT
PH: 7
Temperature: 37
uniprot:Q63704 [carnitine O-palmitoyltransferase(Enzyme) wildtype]

Palmitoyl_CoA + L_Carnitine -> Coenzyme_A + L_Palmitoylcarnitine		 (( (Vmax * B) ) / (Km + B)) buffer: 75 mM KCl, 80 mM Mannitol, 1 mM EGTA, 1 mM DTT
PH: 7
Temperature: 37
uniprot:P50416 [carnitine O-palmitoyltransferase(Enzyme) wildtype isoenzyme CPT1A]

L_Carnitine + Palmitoyl_CoA -> Coenzyme_A + L_Palmitoylcarnitine		 (( (Vmax * S2) ) / (Km + S2)) buffer: 105 mM Tris-HCl, 0.25 mM reduced Glutathione, 4 mM ATP, 4 mM MgCl2, 15 mM KCl, 2 mM KCN, 40 mg/l Rotenone, 10 mg/ml Albumin
PH: 7.4
Temperature: 30
uniprot:Q63704 [carnitine O-palmitoyltransferase(Enzyme) wildtype isoenzyme CPT1B]

L_Carnitine + Palmitoyl_CoA -> Coenzyme_A + L_Palmitoylcarnitine		 (( (Vmax * S1) ) / (Km + S1)) buffer: 105 mM Tris-HCl, 0.25 mM reduced Glutathione, 4 mM ATP, 4 mM MgCl2, 15 mM KCl, 2 mM KCN, 40 µg/ml Rotenone, 10 mg/ml Albumin
PH: 7.4
Temperature: 30
uniprot:P32198 [carnitine O-palmitoyltransferase(Enzyme) wildtype isoenzyme CPT1A]

L_Carnitine + Palmitoyl_CoA -> Coenzyme_A + L_Palmitoylcarnitine		 (( (Vmax * S1) ) / (Km + S1)) buffer: 105 mM Tris-HCl, 0.25 mM reduced Glutathione, 4 mM ATP, 4 mM MgCl2, 15 mM KCl, 2 mM KCN, 40 µg/ml Rotenone, 10 mg/ml Albumin
PH: 7.4
Temperature: 30
uniprot:Q63704 [carnitine O-palmitoyltransferase(Enzyme) wildtype isoenzyme CPT1B]

L_Carnitine + Palmitoyl_CoA -> L_Palmitoylcarnitine + Coenzyme_A		 (( (Vmax * S2) ) / (Km + S2)) buffer: 105 mM Tris-HCl, 0.25 mM reduced Glutathione, 4 mM ATP, 4 mM MgCl2, 15 mM KCl, 2 mM KCN, 40 µg/ml Rotenone, 10 mg/ml Albumin
PH: 7.4
Temperature: 30
- [carnitine O-palmitoyltransferase(Enzyme) wildtype isoenzyme CPT1B]

Palmitoyl_CoA + L_Carnitine -> L_Palmitoylcarnitine + Coenzyme_A		 (( (Vmax * S2) ) / (Km + S2)) buffer: 105 mM Tris-HCl, 0.25 mM reduced Glutathione, 4 mM ATP, 4 mM MgCl2, 15 mM KCl, 2 mM KCN, 40 µg/ml Rotenone, 10 mg/ml Albumin
PH: 7.4
Temperature: 30
- [carnitine O-palmitoyltransferase(Enzyme) wildtype isoenzyme CPT1B]

L_Carnitine + Palmitoyl_CoA -> Coenzyme_A + L_Palmitoylcarnitine		 (( (Vmax * S2) ) / (Km + S2)) buffer: 105 mM Tris-HCl, 0.25 mM reduced Glutathione, 4 mM ATP, 4 mM MgCl2, 15 mM KCl, 2 mM KCN, 40 µg/ml Rotenone, 10 mg/ml Albumin
PH: 7.4
Temperature: 30
uniprot:Q92523 [carnitine O-palmitoyltransferase(Enzyme) wildtype isoenzyme CPT1B]

Palmitoyl_CoA + L_Carnitine -> L_Palmitoylcarnitine + Coenzyme_A		 (( (Vmax * S2) ) / (Km + S2)) buffer: 105 mM Tris-HCl, 0.25 mM reduced Glutathione, 4 mM ATP, 4 mM MgCl2, 15 mM KCl, 2 mM KCN, 40 µg/ml Rotenone, 10 mg/ml Albumin
PH: 7.4
Temperature: 30
uniprot:Q63704 [carnitine O-palmitoyltransferase(Enzyme) wildtype isoenzyme CPT1B]

L_Carnitine + Palmitoyl_CoA -> Coenzyme_A + L_Palmitoylcarnitine		 (( (Vmax * S2) ) / (Km + S2)) buffer: 105 mM Tris-HCl, 0.25 mM reduced Glutathione, 4 mM ATP, 4 mM MgCl2, 15 mM KCl, 2 mM KCN, 40 µg/ml Rotenone, 10 mg/ml Albumin
PH: 7.4
Temperature: 30
uniprot:P50416 [carnitine O-palmitoyltransferase(Enzyme) wildtype isoenzyme CPT1A]

L_Carnitine + Palmitoyl_CoA -> Coenzyme_A + L_Palmitoylcarnitine		 (( (Vmax * S2) ) / (Km + S2)) buffer: 105 mM Tris-HCl, 0.25 mM reduced Glutathione, 4 mM ATP, 4 mM MgCl2, 15 mM KCl, 2 mM KCN, 40 µg/ml Rotenone, 10 mg/ml Albumin
PH: 7.4
Temperature: 30
uniprot:P32198 [carnitine O-palmitoyltransferase(Enzyme) wildtype isoenzyme CPT1A]

L_Carnitine + Palmitoyl_CoA -> Coenzyme_A + L_Palmitoylcarnitine		 (( (Vmax * S2) ) / (Km + S2)) buffer: 105 mM Tris-HCl, 0.25 mM reduced Glutathione, 4 mM ATP, 4 mM MgCl2, 15 mM KCl, 2 mM KCN, 40 µg/ml Rotenone, 10 mg/ml Albumin
PH: 7.4
Temperature: 30
uniprot:Q63704 [carnitine O-palmitoyltransferase(Enzyme) wildtype isoenzyme CPT1B]

Palmitoyl_CoA + L_Carnitine -> Coenzyme_A + L_Palmitoylcarnitine		 (( (Vmax * S2) ) / (Km + S2)) buffer: 105 mM Tris-HCl, 0.25 mM reduced Glutathione, 4 mM ATP, 4 mM MgCl2, 15 mM KCl, 2 mM KCN, 40 µg/ml Rotenone, 10 mg/ml Albumin
PH: 7.4
Temperature: 30
- [carnitine O-palmitoyltransferase(Enzyme) wildtype isoenzyme CPT1B]

L_Carnitine + Palmitoyl_CoA -> L_Palmitoylcarnitine + Coenzyme_A		 (( (Vmax * S2) ) / (Km + S2)) buffer: 105 mM Tris-HCl, 0.25 mM reduced Glutathione, 4 mM ATP, 4 mM MgCl2, 15 mM KCl, 2 mM KCN, 40 µg/ml Rotenone, 10 mg/ml Albumin
PH: 7.4
Temperature: 30
- [carnitine O-palmitoyltransferase(Enzyme) wildtype isoenzyme CPT1B]

Palmitoyl_CoA + L_Carnitine -> Coenzyme_A + L_Palmitoylcarnitine		 (( (Vmax * S2) ) / (Km + S2)) buffer: 105 mM Tris-HCl, 0.25 mM reduced Glutathione, 4 mM ATP, 4 mM MgCl2, 15 mM KCl, 2 mM KCN, 40 µg/ml Rotenone, 10 mg/ml Albumin
PH: 7.4
Temperature: 30
- [carnitine O-palmitoyltransferase(Enzyme) wildtype isoenzyme CPT1A]

Palmitoyl_CoA + L_Carnitine -> L_Palmitoylcarnitine + Coenzyme_A		 (( (Vmax * S2) ) / (Km + S2)) buffer: 105 mM Tris-HCl, 0.25 mM reduced Glutathione, 4 mM ATP, 4 mM MgCl2, 15 mM KCl, 2 mM KCN, 40 µg/ml Rotenone, 10 mg/ml Albumin
PH: 7.4
Temperature: 30

Pathways

pathway id name
BioCyc:HUMAN_PWY-6111 mitochondrial L-carnitine shuttle
BioCyc:META_PWY-6111 mitochondrial L-carnitine shuttle
Reactome:R-HSA-9909396 Circadian clock
Reactome:R-HSA-9933387 RORA,B,C and NR1D1 (REV-ERBA) regulate gene expression
Reactome:R-BTA-1430728 Metabolism
Reactome:R-BTA-556833 Metabolism of lipids
Reactome:R-DRE-8978868 Fatty acid metabolism
Reactome:R-HSA-400206 Regulation of lipid metabolism by PPARalpha
Reactome:R-MMU-1430728 Metabolism
Reactome:R-MMU-556833 Metabolism of lipids
Reactome:R-RNO-8978868 Fatty acid metabolism
Reactome:R-XTR-8978868 Fatty acid metabolism
Reactome:R-BTA-8978868 Fatty acid metabolism
Reactome:R-DRE-200425 Carnitine shuttle
Reactome:R-HSA-556833 Metabolism of lipids
Reactome:R-HSA-8978868 Fatty acid metabolism
Reactome:R-MMU-8978868 Fatty acid metabolism
Reactome:R-RNO-200425 Carnitine shuttle
Reactome:R-DRE-1430728 Metabolism
Reactome:R-DRE-556833 Metabolism of lipids
Reactome:R-HSA-1989781 PPARA activates gene expression
Reactome:R-RNO-1430728 Metabolism
Reactome:R-RNO-556833 Metabolism of lipids
Reactome:R-XTR-1430728 Metabolism
Reactome:R-XTR-556833 Metabolism of lipids
Reactome:R-BTA-200425 Carnitine shuttle
Reactome:R-HSA-1430728 Metabolism
Reactome:R-HSA-200425 Carnitine shuttle
Reactome:R-MMU-200425 Carnitine shuttle
Reactome:R-XTR-200425 Carnitine shuttle
WikiPathways:WP4742 Ketogenesis and ketolysis
WikiPathways:WP5181 Roles of ceramides in development of insulin resistance
WikiPathways:WP2318 Fatty acid oxidation
PathBank:SMP0120476 Ethylmalonic Encephalopathy
PathBank:SMP0120520 Short-Chain Acyl-CoA Dehydrogenase Deficiency (SCAD Deficiency)
PathBank:SMP0120581 Adrenoleukodystrophy, X-Linked
PathBank:SMP0120605 Very-Long-Chain Acyl-CoA Dehydrogenase Deficiency (VLCAD)
PathBank:SMP0120609 Trifunctional Protein Deficiency
PathBank:SMP0000051 Fatty Acid Metabolism
PathBank:SMP0000235 Short-Chain Acyl-CoA Dehydrogenase Deficiency (SCAD Deficiency)
PathBank:SMP0000052 Beta Oxidation of Very Long Chain Fatty Acids
PathBank:SMP0000482 Mitochondrial Beta-Oxidation of Long Chain Saturated Fatty Acids
PathBank:SMP0000516 Adrenoleukodystrophy, X-Linked
PathBank:SMP0000540 Very-Long-Chain Acyl-CoA Dehydrogenase Deficiency (VLCAD)
PathBank:SMP0120604 Long Chain Acyl-CoA Dehydrogenase Deficiency (LCAD)
PathBank:SMP0000185 Glutaric Aciduria Type I
PathBank:SMP0000539 Long Chain Acyl-CoA Dehydrogenase Deficiency (LCAD)
PathBank:SMP0087172 Fatty Acid Metabolism
PathBank:SMP0120582 Carnitine-Acylcarnitine Translocase Deficiency
PathBank:SMP0120606 Carnitine Palmitoyl Transferase Deficiency II
PathBank:SMP0000030 Oxidation of Branched-Chain Fatty Acids
PathBank:SMP0000517 Carnitine-Acylcarnitine Translocase Deficiency
PathBank:SMP0000541 Carnitine Palmitoyl Transferase Deficiency II
PathBank:SMP0000545 Trifunctional Protein Deficiency
PathBank:SMP0086926 Oxidation of Branched-Chain Fatty Acids
PathBank:SMP0063598 Beta Oxidation of Very Long Chain Fatty Acids
PathBank:SMP0120603 Carnitine Palmitoyl Transferase Deficiency I
PathBank:SMP0120607 Medium Chain Acyl-CoA Dehydrogenase Deficiency (MCAD)
PathBank:SMP0000181 Ethylmalonic Encephalopathy
PathBank:SMP0000538 Carnitine Palmitoyl Transferase Deficiency I
PathBank:SMP0000542 Medium Chain Acyl-CoA Dehydrogenase Deficiency (MCAD)
PathBank:SMP0087255 Oxidation of Branched-Chain Fatty Acids
PathBank:SMP0087259 Beta Oxidation of Very Long Chain Fatty Acids
PathBank:SMP0087267 Mitochondrial Beta-Oxidation of Long Chain Saturated Fatty Acids
PathBank:SMP0063589 Fatty Acid Metabolism
PathBank:SMP0063639 Mitochondrial Beta-Oxidation of Long Chain Saturated Fatty Acids