EC: 2.3.1.1

amino-acid N-acetyltransferase (acetyl-CoA:L-glutamate N-acetyltransferase)

enzyme lambda metabolic reaction experiment
uniprot:Q9Z4S1 [amino-acid N-acetyltransferase(Enzyme) wildtype N-terminal His-tag]

L_Glutamate + Acetyl_CoA -> Coenzyme_A + N_Acetyl_L_glutamate		 (( (Vmax * S) ) / (Km + S)) buffer: 0.1 M MES, 0.1 M Pipes, 0.1 M Tris, 0.1 M glycine, 0.1 M K2HPO4
PH: 7.5
Temperature: 90
uniprot:O33289 [L-Arginine]

Acetyl_CoA + L_Glutamine -> Coenzyme_A + N_Acetyl_L_glutamine		 (( (kcat * E) ) / (1 + (( (I / IC50) ) ^ s))) buffer: 40 mM TEA, 100 mM ammonium sulfate, 500 mM NaCl, 0.2 mM DTNB
PH: 8
Temperature: 25
uniprot:O33289 [amino-acid N-acetyltransferase(Enzyme) wildtype]

Acetyl_CoA + L_Glutamine -> Coenzyme_A + N_Acetyl_L_glutamine		 (( (( (( (kcat * E) ) * A) ) * B) ) / (( (( (( (Kia * Kb) ) + (Ka * B)) ) + (Kb * A)) ) + (A * B))) buffer: 40 mM TEA, 100 mM ammonium sulfate, 500 mM NaCl, 0.2 mM DTNB
PH: 8
Temperature: 25
uniprot:O33289 [amino-acid N-acetyltransferase(Enzyme) wildtype]

L_Glutamine + Acetyl_CoA -> N_Acetyl_L_glutamine + Coenzyme_A		 (( (( (( (kcat * E) ) * A) ) * B) ) / (( (( (( (Kia * Kb) ) + (Ka * B)) ) + (Kb * A)) ) + (A * B))) buffer: 40 mM TEA, 100 mM ammonium sulfate, 500 mM NaCl, 0.2 mM DTNB
PH: 8
Temperature: 25
uniprot:Q07908 [amino-acid N-acetyltransferase(Enzyme) wildtype N-terminal His-tag]

Acetyl_CoA + L_Glutamate -> N_Acetyl_L_glutamate + Coenzyme_A		 (( (Vmax * S) ) / (Km + S)) buffer: 0.1 M MES, 0.1 M Pipes, 0.1 M Tris, 0.1 M glycine, 0.1 M K2HPO4
PH: 8
Temperature: 75
uniprot:P0A6C5 [amino-acid N-acetyltransferase(Enzyme) wildtype]

Acetyl_CoA + L_Glutamate -> N_Acetyl_L_glutamate + Coenzyme_A		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM Tris-HCl, 10 mM ATP, 20 mM MgCl2, 0.2 mM NADPH, 20 µg/ml N-Acetyl-glutamate kinase, 50 µg/ml N-Acetyl-gamma-glutamyl-phosphate reductase, 38 µg/ml crude extract
PH: 9
Temperature: 30
uniprot:P0A6C5 [amino-acid N-acetyltransferase(Enzyme) wildtype]

Acetyl_CoA + L_Glutamate -> Coenzyme_A + N_Acetyl_L_glutamate		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM Tris-HCl, 10 mM ATP, 20 mM MgCl2, 0.2 mM NADPH, 20 µg/ml N-Acetyl-glutamate kinase, 50 µg/ml N-Acetyl-gamma-glutamyl-phosphate reductase, 38 µg/ml crude extract
PH: 9
Temperature: 30
- [amino-acid N-acetyltransferase(Enzyme) wildtype]

L_Glutamate + Acetyl_CoA -> N_Acetyl_L_glutamate + Coenzyme_A		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Tris-HCl, 5.0 EDTA
PH: 8.5
Temperature: 25
- [amino-acid N-acetyltransferase(Enzyme) wildtype]

Acetyl_CoA + L_Glutamate -> N_Acetyl_L_glutamate + Coenzyme_A		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 5.0 EDTA
PH: 8.5
Temperature: 25

Pathways

pathway id name
BioCyc:ECO_GLUTORN-PWY L-ornithine biosynthesis I
BioCyc:ARA_GLUTORN-PWY L-ornithine biosynthesis I
BioCyc:MTBH37RV_GLUTORN-PWY L-ornithine biosynthesis I
BioCyc:MTBCDC1551_GLUTORN-PWY L-ornithine biosynthesis I
BioCyc:ECOO157_GLUTORN-PWY ornithine biosynthesis
BioCyc:ANTHRA_ARGSYN-PWY L-arginine biosynthesis I (via L-ornithine)
BioCyc:AGRO_GLUTORN-PWY L-ornithine biosynthesis I
BioCyc:ECOL316407_GLUTORN-PWY ornithine biosynthesis
BioCyc:ECOL316407_ARG+POLYAMINE-SYN superpathway of arginine and polyamine biosynthesis
BioCyc:ECO_ARG+POLYAMINE-SYN superpathway of arginine and polyamine biosynthesis
BioCyc:ECO_ARGSYN-PWY L-arginine biosynthesis I (via L-ornithine)
BioCyc:MTBH37RV_ARGSYNBSUB-PWY L-arginine biosynthesis II (acetyl cycle)
BioCyc:MTBCDC1551_ARGSYN-PWY L-arginine biosynthesis I (via L-ornithine)
BioCyc:CAULO_GLUTORN-PWY ornithine biosynthesis
BioCyc:ANTHRA_ARGSYNBSUB-PWY L-arginine biosynthesis II (acetyl cycle)
BioCyc:ANTHRA_GLUTORN-PWY L-ornithine biosynthesis I
BioCyc:AGRO_ARGSYN-PWY L-arginine biosynthesis I (via L-ornithine)
BioCyc:PCHR_GLUTORN-PWY ornithine biosynthesis
BioCyc:CORYNE_ARGSYNBSUB-PWY L-arginine biosynthesis II (acetyl cycle)
BioCyc:META_ARGSYN-PWY L-arginine biosynthesis I (via L-ornithine)
BioCyc:META_GLUTORN-PWY L-ornithine biosynthesis I
BioCyc:ARA_ARGSYN-PWY L-arginine biosynthesis I (via L-ornithine)
BioCyc:CAULO_ARGSYNBSUB-PWY arginine biosynthesis II (acetyl cycle)
BioCyc:ECOL316407_ARGSYN-PWY arginine biosynthesis I (via L-ornithine)
BioCyc:META_ARG+POLYAMINE-SYN superpathway of arginine and polyamine biosynthesis
BioCyc:YEAST_ARGSYNBSUB-PWY arginine biosynthesis
BioCyc:ARA_ARGSYNBSUB-PWY L-arginine biosynthesis II (acetyl cycle)
BioCyc:MTBH37RV_ARGSYN-PWY L-arginine biosynthesis I (via L-ornithine)
BioCyc:MTBCDC1551_ARGSYNBSUB-PWY L-arginine biosynthesis II (acetyl cycle)
BioCyc:ECOO157_ARGSYN-PWY arginine biosynthesis I (via L-ornithine)
BioCyc:CAULO_ARGSYN-PWY arginine biosynthesis I
BioCyc:BSUB_ARGSYNBSUB-PWY L-arginine biosynthesis II (acetyl cycle)
BioCyc:PCHR_ARGSYN-PWY arginine biosynthesis I
BioCyc:PCHR_ARGSYNBSUB-PWY arginine biosynthesis II (acetyl cycle)
Plant Reactome:R-OSA-2744345 Metabolism and regulation
Plant Reactome:R-OSA-2744343 Amino acid metabolism
Plant Reactome:R-OSA-1119263 Arginine biosynthesis
Plant Reactome:R-OSA-5655122 Amino acid biosynthesis
Plant Reactome:R-OSA-1119622 Arginine biosynthesis II (acetyl cycle)
PlantCyc:ARA_ARGSYN-PWY L-arginine biosynthesis I (via L-ornithine)
PlantCyc:ARA_GLUTORN-PWY L-ornithine biosynthesis I
PlantCyc:ARA_ARGSYNBSUB-PWY L-arginine biosynthesis II (acetyl cycle)
Reactome:R-HSA-9955698 Diseases of the urea cycle
Reactome:R-HSA-9955693 NAGS variants cause NAGS deficiency
Reactome:R-DRE-71291 Amino acid and derivative metabolism
Reactome:R-HSA-1643685 Disease
Reactome:R-MMU-1430728 Metabolism
Reactome:R-DRE-1430728 Metabolism
Reactome:R-DRE-70635 Urea cycle
Reactome:R-HSA-71291 Amino acid and derivative metabolism
Reactome:R-HSA-5668914 Diseases of metabolism
Reactome:R-MMU-71291 Amino acid and derivative metabolism
Reactome:R-HSA-1430728 Metabolism
Reactome:R-HSA-70635 Urea cycle
Reactome:R-MMU-70635 Urea cycle
WikiPathways:WP4595 Urea cycle and associated pathways
WikiPathways:WP497 Urea cycle and metabolism of amino groups
WikiPathways:WP4583 Biomarkers for urea cycle disorders
PathBank:SMP0002420 Proline Metabolism
PathBank:SMP0002416 Arginine Metabolism