arginine biosynthesis

Pauwels K, Abadjieva A, Hilven P, Stankiewicz A, Crabeel M. TheN-acetylglutamate synthase/N-acetylglutamate kinase metabolon ofSaccharomyces cerevisiaeallows co-ordinated feedback regulation of the first two steps in arginine biosynthesis. European Journal of Biochemistry. 2003 Mar;270(5):1014–24. doi: 10.1046/j.1432-1033.2003.03477.x.; Abadjieva A, Pauwels K, Hilven P, Crabeel M. A new yeast metabolon involving at least the two first enzymes of arginine biosynthesis: acetylglutamate synthase activity requires complex formation with acetylglutamate kinase. J Biol Chem. 2001 Nov 16;276(46):42869–80. doi: 10.1074/jbc.m103732200. PMID: 11553611.; Jauniaux JC, Urrestarazu LA, Wiame JM. Arginine metabolism in Saccharomyces cerevisiae: subcellular localization of the enzymes. J Bacteriol. 1978 Mar;133(3):1096–107. doi: 10.1128/jb.133.3.1096-1107.1978.

Metabolites

Diphosphate

Formula: H4P2O7 (177.9432294)

CAS ID: 2466-09-3

H+

Formula: H (1.0078246)

CAS ID: 12408-02-5

H2O

Formula: H2O (18.0105642)

CAS ID: 7732-18-5

HCO3-

Formula: HCO3 (60.9925696)

CAS ID: 71-52-3

L-argininium(1+)

Formula: C6H15N4O2 (175.119495)

CAS ID: 17806-42-7

2-oxoglutarate(2-)

Formula: C5H4O5 (144.00587339999998)

CAS ID: 64-15-3

butenedioate

Formula: C4H2O4 (113.99530920000001)

CAS ID: 17013-01-3

L-aspartate(1-)

Formula: C4H6NO4 (132.0296816)

CAS ID:

adenosine 5'-monophosphate(2-)

Formula: C10H12N5O7P (345.0474332)

CAS ID:

NADPH(4-)

Formula: C21H26N7O17P3 (741.0598016)

CAS ID: 53-57-6

ATP(4-)

Formula: C10H12N5O13P3 (502.9644492)

CAS ID:

ADP(3-)

Formula: C10H12N5O10P2 (424.0059412)

CAS ID:

NADP(3-)

Formula: C21H25N7O17P3 (740.051977)

CAS ID: 53-59-8



Enzyme

EC Number name full name note
1.2.1.38 N-acetyl-gamma-glutamyl-phosphate reductase N-acetyl-L-glutamate-5-semialdehyde:NADP+ 5-oxidoreductase (phosphorylating)
2.1.3.3 ornithine carbamoyltransferase carbamoyl-phosphate:L-ornithine carbamoyltransferase
2.3.1.1 amino-acid N-acetyltransferase acetyl-CoA:L-glutamate N-acetyltransferase
2.3.1.35 glutamate N-acetyltransferase N2-acetyl-L-ornithine:L-glutamate N-acetyltransferase
2.6.1.11 acetylornithine transaminase N2-acetyl-L-ornithine:2-oxoglutarate 5-aminotransferase
2.7.2.8 acetylglutamate kinase ATP:N-acetyl-L-glutamate 5-phosphotransferase
4.3.2.1 argininosuccinate lyase 2-(Nomega-L-arginino)succinate arginine-lyase (fumarate-forming)
6.3.4.16 carbamoyl-phosphate synthase (ammonia) carbon-dioxide:ammonia ligase (ADP-forming, carbamate-phosphorylating)
6.3.4.5 argininosuccinate synthase L-citrulline:L-aspartate ligase (AMP-forming)
6.3.5.5 carbamoyl-phosphate synthase (glutamine-hydrolysing) hydrogen-carbonate:L-glutamine amido-ligase (ADP-forming, carbamate-phosphorylating)


Proteins

Protein ID name full name
P03965 CPA2 Carbamoyl phosphate synthase arginine-specific large chain
P04076 ARG4 Argininosuccinate lyase
P05150 ARG3 Ornithine carbamoyltransferase
P07258 CPA1 Carbamoyl phosphate synthase arginine-specific small chain
P18544 ARG8 Acetylornithine aminotransferase, mitochondrial
P22768 ARG1 Argininosuccinate synthase
P40360 ARG2 Amino-acid acetyltransferase, mitochondrial
Q01217 ARG5,6 Protein ARG5,6, mitochondrial
Q04728 ARG7 Arginine biosynthesis bifunctional protein ArgJ, mitochondrial