EC: 1.3.5.1

succinate dehydrogenase (succinate:quinone oxidoreductase)

enzyme lambda metabolic reaction experiment
- [Al3+]

Acceptor + Succinate -> Reduced_acceptor + Fumarate		 (( (Vmax * S) ) / (Km + S)) buffer:
PH: 7
Temperature: 37
- [succinate dehydrogenase (ubiquinone)(Enzyme) wildtype]

Succinate + Acceptor -> Fumarate + Reduced_acceptor		 (( (Vmax * S) ) / (Km + S)) buffer:
PH: 7
Temperature: 37
uniprot:P0C278 [succinate dehydrogenase (ubiquinone)(Enzyme) wildtype]

Fumarate + FADH2 -> Succinate + FAD		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: TrisHCl, 0.45 M NaCl, 0.2 mM methyl viologen
PH: 7.2
Temperature: 25
uniprot:P0C278 [succinate dehydrogenase (ubiquinone)(Enzyme) mutant A251C:S430C nonbridged]

FADH2 + Fumarate -> Succinate + FAD		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: MES, 0.45 M NaCl, 0.2 mM methyl viologen, 1 mM DTT
PH: 6
Temperature: 25
uniprot:P0C278 [succinate dehydrogenase (ubiquinone)(Enzyme) wildtype]

FADH2 + Fumarate -> Succinate + FAD		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: MES, 0.45 M NaCl, 0.2 mM methyl viologen,
PH: 6
Temperature: 25
uniprot:P0C278 [succinate dehydrogenase (ubiquinone)(Enzyme) mutant A251C:S430C disulfide-bridged]

Succinate + FAD -> Fumarate + FADH2		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: MES, 0.45 M NaCl, 0.2 mM methyl viologen
PH: 6
Temperature: 25
uniprot:P0C278 [succinate dehydrogenase (ubiquinone)(Enzyme) mutant A251C:S430C nonbridged]

FADH2 + Fumarate -> FAD + Succinate		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: TrisHCl, 0.45 M NaCl, 0.2 mM methyl viologen, 1 mM DTT
PH: 7.2
Temperature: 25
uniprot:P0C278 [succinate dehydrogenase (ubiquinone)(Enzyme) wildtype]

Fumarate + FADH2 -> FAD + Succinate		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: TrisHCl, 0.45 M NaCl, 0.2 mM methyl viologen
PH: 7.5
Temperature: 25
uniprot:P0C278 [succinate dehydrogenase (ubiquinone)(Enzyme) wildtype]

FADH2 + Fumarate -> FAD + Succinate		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: CHES, 0.45 M NaCl, 0.2 mM methyl viologen
PH: 9
Temperature: 25
uniprot:P0C278 [succinate dehydrogenase (ubiquinone)(Enzyme) mutant A251C:S430C disulfide-bridged]

FAD + Succinate -> FADH2 + Fumarate		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: TrisHCl, 0.45 M NaCl, 0.2 mM methyl viologen
PH: 7.2
Temperature: 25
uniprot:P0C278 [succinate dehydrogenase (ubiquinone)(Enzyme) mutant A251C:S430C nonbridged]

FADH2 + Fumarate -> FAD + Succinate		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: TrisHCl, 0.45 M NaCl, 0.2 mM methyl viologen, 1 mM DTT
PH: 7.5
Temperature: 25
uniprot:P0C278 [succinate dehydrogenase (ubiquinone)(Enzyme) mutant A251C:S430C disulfide-bridged]

Succinate + FAD -> Fumarate + FADH2		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: CHES, 0.45 M NaCl, 0.2 mM methyl viologen
PH: 9
Temperature: 25
uniprot:P0C278 [succinate dehydrogenase (ubiquinone)(Enzyme) mutant A251C:S430C nonbridged]

Fumarate + FADH2 -> Succinate + FAD		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: CHES, 0.45 M NaCl, 0.2 mM methyl viologen, 1 mM DTT
PH: 9
Temperature: 25
uniprot:P0C278 [succinate dehydrogenase (ubiquinone)(Enzyme) mutant A251C:S430C disulfide-bridged]

Succinate + FAD -> FADH2 + Fumarate		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: TrisHCl, 0.45 M NaCl, 0.2 mM methyl viologen
PH: 7.5
Temperature: 25
- [succinate dehydrogenase (ubiquinone)(Enzyme) wildtype]

Ubiquinone_1 + Succinate -> Fumarate + Ubiquinol		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM potassium phosphate, 10 mM potassium succinate, 2 mM potassium cyanide, 45 µM dichlorophenolindophenol
PH: 8
Temperature: 25
- [succinate dehydrogenase (ubiquinone)(Enzyme) wildtype]

Succinate + Ubiquinone_2 -> Fumarate + Ubiquinol		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM potassium phosphate, 10 mM potassium succinate, 2 mM potassium cyanide, 45 µM dichlorophenolindophenol
PH: 8
Temperature: 25
- [succinate dehydrogenase (ubiquinone)(Enzyme) wildtype]

Ubiquinone_2 + Succinate -> Fumarate + Ubiquinol		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM potassium phosphate, 10 mM potassium succinate, 2 mM potassium cyanide, 45 µM dichlorophenolindophenol
PH: 8
Temperature: 25
- [succinate dehydrogenase (ubiquinone)(Enzyme) wildtype complex II]

Reduced_acceptor + Fumarate -> Acceptor + Succinate		 (( (( (E * kcat) ) * S2) ) / (Km + S2)) buffer: 30 mM phosphate
PH: 7.5
Temperature: 25

Pathways

pathway id name
BioCyc:META_PWY0-1329 succinate to cytochrome bo oxidase electron transfer
BioCyc:META_PWY-5690 TCA cycle II (plants and fungi)
BioCyc:META_PWY-7279 aerobic respiration II (cytochrome c) (yeast)
BioCyc:META_PWY-7384 anaerobic energy metabolism (invertebrates, mitochondrial)
BioCyc:META_PWY0-1582 glycerol-3-phosphate to fumarate electron transfer
BioCyc:ECO_TCA-GLYOX-BYPASS superpathway of glyoxylate bypass and TCA
BioCyc:ECO_PWY0-1336 NADH to fumarate electron transfer
BioCyc:YEAST_PWY-7279-YEAST aerobic respiration (cytochrome c)
BioCyc:ARA_PWYQT-4481 TCA cycle variation V (plant)
BioCyc:ARA_PWY-3781 aerobic respiration I (cytochrome c)
BioCyc:HPY_PYRIDNUCSYN-PWY NAD biosynthesis I (from aspartate)
BioCyc:ECOO157_TCA TCA cycle I (prokaryotic)
BioCyc:ECOO157_GLYCOLYSIS-TCA-GLYOX-BYPASS superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass
BioCyc:ECOO157_PWY0-1329 succinate to cytochrome bo oxidase electron transfer
BioCyc:ECOL199310_ANARESP1-PWY respiration (anaerobic)
BioCyc:MOUSE_PWY-3781 aerobic respiration -- electron donor II
BioCyc:MOUSE_TCA-1 TCA cycle
BioCyc:ECOL316407_TCA TCA cycle I (prokaryotic)
BioCyc:ECOL316407_PWY0-1336 NADH to fumarate electron transfer
BioCyc:META_PWY-5464 superpathway of cytosolic glycolysis (plants), pyruvate dehydrogenase and TCA cycle
BioCyc:META_PWY0-1353 succinate to cytochrome bd oxidase electron transfer
BioCyc:META_PWY-7389 superpathway of anaerobic energy metabolism (invertebrates)
BioCyc:META_PWY0-1576 hydrogen to fumarate electron transfer
BioCyc:ECO_PWY0-1576 hydrogen to fumarate electron transfer
BioCyc:YEAST_PWY3O-188 aerobic respiration (linear view)
BioCyc:HPY_PWY0-1582 glycerol-3-phosphate to fumarate electron transfer
BioCyc:HPY_REDCITCYC TCA cycle VIII (helicobacter)
BioCyc:ECOO157_FERMENTATION-PWY mixed acid fermentation
BioCyc:ECOO157_TCA-GLYOX-BYPASS superpathway of glyoxylate bypass and TCA
BioCyc:ECOL199310_FERMENTATION-PWY mixed acid fermentation
BioCyc:MOUSE_PWY-5690-1 TCA cycle variation III (eukaryotic)
BioCyc:BSUB_TCA TCA cycle I (prokaryotic)
BioCyc:ECOL316407_PWY0-1353 succinate to cytochrome bd oxidase electron transfer
BioCyc:ECOL316407_ANARESP1-PWY respiration (anaerobic)
BioCyc:HUMAN_PWY66-407 superpathway of conversion of glucose to acetyl CoA and entry into the TCA cycle
BioCyc:HUMAN_PWY66-398 TCA cycle
BioCyc:META_PWY0-1336 NADH to fumarate electron transfer
BioCyc:META_PWY66-398 TCA cycle III (animals)
BioCyc:META_REDCITCYC TCA cycle VIII (helicobacter)
BioCyc:ECO_TCA TCA cycle I (prokaryotic)
BioCyc:ECO_GLYCOLYSIS-TCA-GLYOX-BYPASS superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass
BioCyc:ARA_PWY-4302 aerobic respiration III (alternative oxidase pathway)
BioCyc:MTBH37RV_FERMENTATION-PWY mixed acid fermentation
BioCyc:MTBH37RV_PWY0-1582 glycerol-3-phosphate to fumarate electron transfer
BioCyc:ECOO157_PWY0-1353 succinate to cytochrome bd oxidase electron transfer
BioCyc:ECOL316407_FERMENTATION-PWY mixed acid fermentation
BioCyc:ECOL316407_GLYCOLYSIS-TCA-GLYOX-BYPASS superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass
BioCyc:META_PWY-561 superpathway of glyoxylate cycle and fatty acid degradation
BioCyc:META_PWY-4302 aerobic respiration III (alternative oxidase pathway)
BioCyc:META_PWY-3781 aerobic respiration I (cytochrome c)
BioCyc:META_FERMENTATION-PWY mixed acid fermentation
BioCyc:ECO_FERMENTATION-PWY mixed acid fermentation
BioCyc:ECO_PWY0-1582 glycerol-3-phosphate to fumarate electron transfer
BioCyc:ECO_PWY0-1353 succinate to cytochrome bd oxidase electron transfer
BioCyc:ECO_PWY0-1329 succinate to cytochrome bo oxidase electron transfer
BioCyc:ARA_PWY-5690 TCA cycle II (plants and fungi)
BioCyc:ARA_PWY-561 superpathway of glyoxylate cycle and fatty acid degradation
BioCyc:MTBCDC1551_FERMENTATION-PWY mixed acid fermentation
BioCyc:MTBCDC1551_REDCITCYC TCA cycle VIII (helicobacter)
BioCyc:ECOO157_ANARESP1-PWY respiration (anaerobic)
BioCyc:ECOO157_PWY-3781 aerobic respiration (cytochrome c)
BioCyc:MOUSE_AERESPDON-PWY aerobic respiration -- electron donors reaction list
BioCyc:ECOL316407_PWY0-1329 succinate to cytochrome bo oxidase electron transfer
BioCyc:ECOL316407_TCA-GLYOX-BYPASS superpathway of glyoxylate bypass and TCA
WikiPathways:WP2472 Escherichia coli K-12 peripherome
PathBank:SMP0000802 TCA Cycle
PathBank:SMP0000808 Inner Membrane Transport
PathBank:SMP0000936 Oxidative Phosphorylation
PathBank:SMP0001018 TCA cycle (ubiquinol-2)
PathBank:SMP0001019 TCA cycle (ubiquinol-3)
PathBank:SMP0001020 TCA cycle (ubiquinol-4)
PathBank:SMP0001021 TCA cycle (ubiquinol-5)
PathBank:SMP0001022 TCA cycle (ubiquinol-6)
PathBank:SMP0001023 TCA cycle (ubiquinol-7)
PathBank:SMP0001024 TCA cycle (ubiquinol-8)
PathBank:SMP0001025 TCA cycle (ubiquinol-9)
PathBank:SMP0001026 TCA cycle (ubiquinol-10)
PathBank:SMP0002037 TCA cycle (ubiquinol-0)
Plant Reactome:R-OSA-1119533 TCA cycle (plant)
Plant Reactome:R-OSA-2744345 Metabolism and regulation
Plant Reactome:R-OSA-2961031 Generation of precursor metabolites and energy
PlantCyc:ARA_PWY-4302 aerobic respiration III (alternative oxidase pathway)
PlantCyc:ARA_PWY-5464 superpathway of cytosolic glycolysis (plants), pyruvate dehydrogenase and TCA cycle
PlantCyc:PLANT_PWY-3781 aerobic respiration I (cytochrome c)
PlantCyc:ARA_PWY-3781 aerobic respiration I (cytochrome c)
PlantCyc:ARA_PWY-561 superpathway of glyoxylate cycle and fatty acid degradation
PlantCyc:PLANT_PWYQT-4481 TCA cycle variation V (plant)
PlantCyc:ARA_PWYQT-4481 TCA cycle variation V (plant)
PlantCyc:ARA_PWY-5690 TCA cycle II (plants and fungi)
PlantCyc:PLANT_PWY-5690 TCA cycle II (plants and fungi)
PlantCyc:PLANT_PWY-4302 aerobic respiration III (alternative oxidase pathway)
PlantCyc:PLANT_PWY-5464 superpathway of cytosolic glycolysis (plants), pyruvate dehydrogenase and TCA cycle
PlantCyc:PLANT_PWY-561 superpathway of glyoxylate cycle and fatty acid degradation
Reactome:R-DRE-9854311 Maturation of TCA enzymes and regulation of TCA cycle
Reactome:R-DDI-9854311 Maturation of TCA enzymes and regulation of TCA cycle
Reactome:R-MMU-9854311 Maturation of TCA enzymes and regulation of TCA cycle
Reactome:R-RNO-9854311 Maturation of TCA enzymes and regulation of TCA cycle
Reactome:R-SSC-9854311 Maturation of TCA enzymes and regulation of TCA cycle
Reactome:R-CEL-9854311 Maturation of TCA enzymes and regulation of TCA cycle
Reactome:R-DME-9854311 Maturation of TCA enzymes and regulation of TCA cycle
Reactome:R-HSA-9854311 Maturation of TCA enzymes and regulation of TCA cycle
Reactome:R-SCE-9854311 Maturation of TCA enzymes and regulation of TCA cycle
Reactome:R-SPO-9854311 Maturation of TCA enzymes and regulation of TCA cycle
Reactome:R-XTR-9854311 Maturation of TCA enzymes and regulation of TCA cycle
Reactome:R-DME-1428517 Aerobic respiration and respiratory electron transport
Reactome:R-DME-71403 Citric acid cycle (TCA cycle)
Reactome:R-GGA-1660598 Metabolism
Reactome:R-GGA-372987 The tricarboxylic acid cycle
Reactome:R-HSA-611105 Respiratory electron transport
Reactome:R-MMU-1430728 Metabolism
Reactome:R-SPO-1430728 Metabolism
Reactome:R-SPO-1428517 Aerobic respiration and respiratory electron transport
Reactome:R-SPO-71403 Citric acid cycle (TCA cycle)
Reactome:R-DME-1430728 Metabolism
Reactome:R-SCE-1430728 Metabolism
Reactome:R-SSC-1430728 Metabolism
Reactome:R-XTR-1428517 Aerobic respiration and respiratory electron transport
Reactome:R-XTR-71403 Citric acid cycle (TCA cycle)
Reactome:R-CEL-1428517 Aerobic respiration and respiratory electron transport
Reactome:R-CEL-71403 Citric acid cycle (TCA cycle)
Reactome:R-DRE-1430728 Metabolism
Reactome:R-DRE-1428517 Aerobic respiration and respiratory electron transport
Reactome:R-DRE-71403 Citric acid cycle (TCA cycle)
Reactome:R-HSA-1428517 Aerobic respiration and respiratory electron transport
Reactome:R-HSA-71403 Citric acid cycle (TCA cycle)
Reactome:R-MMU-1428517 Aerobic respiration and respiratory electron transport
Reactome:R-MMU-71403 Citric acid cycle (TCA cycle)
Reactome:R-RNO-1430728 Metabolism
Reactome:R-RNO-1428517 Aerobic respiration and respiratory electron transport
Reactome:R-RNO-71403 Citric acid cycle (TCA cycle)
Reactome:R-SCE-1428517 Aerobic respiration and respiratory electron transport
Reactome:R-SCE-71403 Citric acid cycle (TCA cycle)
Reactome:R-XTR-1430728 Metabolism
Reactome:R-CEL-1430728 Metabolism
Reactome:R-DDI-1430728 Metabolism
Reactome:R-DDI-1428517 Aerobic respiration and respiratory electron transport
Reactome:R-DDI-71403 Citric acid cycle (TCA cycle)
Reactome:R-HSA-1430728 Metabolism
Reactome:R-SSC-1428517 Aerobic respiration and respiratory electron transport
Reactome:R-SSC-71403 Citric acid cycle (TCA cycle)
WikiPathways:WP2624 TCA cycle (Krebs cycle)
PathBank:SMP0120830 Fumarase Deficiency
PathBank:SMP0120834 Pyruvate Dehydrogenase Deficiency (E2)
PathBank:SMP0120613 2-Ketoglutarate Dehydrogenase Complex Deficiency
PathBank:SMP0121182 Citrate Cycle
PathBank:SMP0000548 Mitochondrial Complex II Deficiency
PathBank:SMP0002295 The Oncogenic Action of Fumarate
PathBank:SMP0002358 The Oncogenic Action of L-2-Hydroxyglutarate in Hydroxyglutaric aciduria
PathBank:SMP0087270 Warburg Effect
PathBank:SMP0087274 Citric Acid Cycle
PathBank:SMP0087354 Mitochondrial Electron Transport Chain
PathBank:SMP0063648 Mitochondrial Electron Transport Chain
PathBank:SMP0120829 Congenital Lactic Acidosis
PathBank:SMP0120833 Pyruvate Dehydrogenase Deficiency (E3)
PathBank:SMP0108771 The Oncogenic Action of 2-Hydroxyglutarate
PathBank:SMP0120612 Mitochondrial Complex II Deficiency
PathBank:SMP0120922 LPS and Citrate Signaling and Inflammation
PathBank:SMP0120946 LPS and Citrate Signaling and Inflammation
PathBank:SMP0120970 LPS and Citrate Signaling and Inflammation
PathBank:SMP0000355 Mitochondrial Electron Transport Chain
PathBank:SMP0000547 Fumarase Deficiency
PathBank:SMP0000551 Pyruvate Dehydrogenase Deficiency (E2)
PathBank:SMP0002292 The Oncogenic Action of Succinate
PathBank:SMP0087261 Mitochondrial Electron Transport Chain
PathBank:SMP0120832 2-Ketoglutarate Dehydrogenase Complex Deficiency
PathBank:SMP0108758 The Oncogenic Action of 2-Hydroxyglutarate
PathBank:SMP0108773 Glutaminolysis and Cancer
PathBank:SMP0120610 Congenital Lactic Acidosis
PathBank:SMP0120614 Pyruvate Dehydrogenase Deficiency (E3)
PathBank:SMP0000057 Citric Acid Cycle
PathBank:SMP0000549 2-Ketoglutarate Dehydrogenase Complex Deficiency
PathBank:SMP0000654 Warburg Effect
PathBank:SMP0002359 The Oncogenic Action of D-2-Hydroxyglutarate in Hydroxyglutaric aciduria
PathBank:SMP0002414 TCA Cycle
PathBank:SMP0087163 Citric Acid Cycle
PathBank:SMP0100046 LPS and Citrate Signaling and Inflammation
PathBank:SMP0120831 Mitochondrial Complex II Deficiency
PathBank:SMP0120611 Fumarase Deficiency
PathBank:SMP0120615 Pyruvate Dehydrogenase Deficiency (E2)
PathBank:SMP0000546 Congenital Lactic Acidosis
PathBank:SMP0000550 Pyruvate Dehydrogenase Deficiency (E3)
PathBank:SMP0002291 The Oncogenic Action of 2-Hydroxyglutarate
PathBank:SMP0002298 Glutaminolysis and Cancer
PathBank:SMP0002304 TCA Cycle
PathBank:SMP0086930 Warburg Effect
PathBank:SMP0087363 Warburg Effect
PathBank:SMP0012092 Oxidative Phosphorylation
PathBank:SMP0063477 Citric Acid Cycle