EC: 2.7.1.35

pyridoxal kinase (ATP:pyridoxal 5'-phosphotransferase)

enzyme lambda metabolic reaction experiment
uniprot:Q55EK9 [pyridoxal kinase(Enzyme) wildtype C-terminal His-tag]

ATP + Pyridoxal -> Pyridoxal_phosphate + ADP
(( (Vmax * A) ) / (Km + A)) buffer: 0.1 M potassium phosphate, 0.1 mM zinc chloride
PH: 6.4
Temperature: 37
uniprot:O00764 [pyridoxal kinase(Enzyme) wildtype]

ATP + Pyridoxine -> Pyridoxine_phosphate + ADP
(( (Vmax * B) ) / (Km_B + B)) buffer: 20 mM Potassium phosphate, 60 µM KCl
PH: 5.75
Temperature: 37
uniprot:O00764 [pyridoxal kinase(Enzyme) wildtype]

ATP + Pyridoxine -> Pyridoxine_phosphate + ADP
(( (Vmax * A) ) / (Km_A + A)) buffer: 20 mM Potassium phosphate, 60 µM KCl
PH: 5.75
Temperature: 37

Pathways

pathway id name
BioCyc:META_PWY-6897 thiamine salvage II
BioCyc:META_PLPSAL-PWY pyridoxal 5'-phosphate salvage I
BioCyc:ECO_PWY-6897 thiamine salvage II
BioCyc:ECOO157_PWY-6910 hydroxymethylpyrimidine salvage
BioCyc:ECOL199310_PWY0-845 superpathway of pyridoxal 5'-phosphate biosynthesis and salvage
BioCyc:MOUSE_PLPSAL-PWY pyridoxal 5'-phosphate salvage pathway
BioCyc:ECOL316407_PLPSAL-PWY pyridoxal 5'-phosphate salvage I
BioCyc:META_PWY0-845 superpathway of pyridoxal 5'-phosphate biosynthesis and salvage
BioCyc:ECOO157_PLPSAL-PWY pyridoxal 5'-phosphate salvage I
BioCyc:ECOO157_PWY0-845 superpathway of pyridoxal 5'-phosphate biosynthesis and salvage
BioCyc:META_PWY-6910 hydroxymethylpyrimidine salvage
BioCyc:META_PWY-7282 4-amino-2-methyl-5-diphosphomethylpyrimidine biosynthesis (yeast)
BioCyc:YEAST_PLPSAL-PWY pyridoxal 5'-phosphate salvage pathway
BioCyc:ECOO157_PWY-6897 thiamin salvage II
BioCyc:ECOL199310_PLPSAL-PWY pyridoxal 5'-phosphate salvage I
BioCyc:BSUB_PLPSAL-PWY pyridoxal 5'-phosphate salvage I
BioCyc:HUMAN_PLPSAL-PWY pyridoxal 5'-phosphate salvage
BioCyc:ECO_PWY-6910 hydroxymethylpyrimidine salvage
BioCyc:ECO_PWY0-845 superpathway of pyridoxal 5'-phosphate biosynthesis and salvage
BioCyc:ECO_PLPSAL-PWY pyridoxal 5'-phosphate salvage I
BioCyc:YEAST_THISYNARA-PWY-1 superpathway of thiamin diphosphate biosynthesis III (eukaryotes)
BioCyc:YEAST_PWY-7282 4-amino-2-methyl-5-phosphomethylpyrimidine biosynthesis
BioCyc:ECOL316407_PWY0-845 superpathway of pyridoxal 5'-phosphate biosynthesis and salvage
Reactome:R-BTA-1430728 Metabolism
Reactome:R-DDI-196849 Metabolism of water-soluble vitamins and cofactors
Reactome:R-HSA-196849 Metabolism of water-soluble vitamins and cofactors
Reactome:R-MMU-1430728 Metabolism
Reactome:R-MMU-196849 Metabolism of water-soluble vitamins and cofactors
Reactome:R-RNO-196849 Metabolism of water-soluble vitamins and cofactors
Reactome:R-SPO-1430728 Metabolism
Reactome:R-SPO-964975 Vitamin B6 activation to pyridoxal phosphate
Reactome:R-BTA-196849 Metabolism of water-soluble vitamins and cofactors
Reactome:R-CEL-196849 Metabolism of water-soluble vitamins and cofactors
Reactome:R-DDI-196854 Metabolism of vitamins and cofactors
Reactome:R-HSA-196854 Metabolism of vitamins and cofactors
Reactome:R-MMU-196854 Metabolism of vitamins and cofactors
Reactome:R-RNO-196854 Metabolism of vitamins and cofactors
Reactome:R-SCE-1430728 Metabolism
Reactome:R-SCE-196849 Metabolism of water-soluble vitamins and cofactors
Reactome:R-BTA-964975 Vitamin B6 activation to pyridoxal phosphate
Reactome:R-CEL-964975 Vitamin B6 activation to pyridoxal phosphate
Reactome:R-RNO-1430728 Metabolism
Reactome:R-SCE-964975 Vitamin B6 activation to pyridoxal phosphate
Reactome:R-SPO-196854 Metabolism of vitamins and cofactors
Reactome:R-BTA-196854 Metabolism of vitamins and cofactors
Reactome:R-CEL-1430728 Metabolism
Reactome:R-CEL-196854 Metabolism of vitamins and cofactors
Reactome:R-DDI-1430728 Metabolism
Reactome:R-DDI-964975 Vitamin B6 activation to pyridoxal phosphate
Reactome:R-HSA-1430728 Metabolism
Reactome:R-HSA-964975 Vitamin B6 activation to pyridoxal phosphate
Reactome:R-MMU-964975 Vitamin B6 activation to pyridoxal phosphate
Reactome:R-RNO-964975 Vitamin B6 activation to pyridoxal phosphate
Reactome:R-SCE-196854 Metabolism of vitamins and cofactors
Reactome:R-SPO-196849 Metabolism of water-soluble vitamins and cofactors
WikiPathways:WP4228 Vitamin B6-dependent and responsive disorders
PathBank:SMP0000017 Vitamin B6 Metabolism
PathBank:SMP0000503 Hypophosphatasia
PathBank:SMP0087333 Vitamin B6 Metabolism
PathBank:SMP0087453 Vitamin B6 Metabolism
PathBank:SMP0087240 Vitamin B6 Metabolism
PathBank:SMP0063472 Vitamin B6 Metabolism
PathBank:SMP0120787 Hypophosphatasia