EC: 1.14.11.65

[histone H3]-dimethyl-L-lysine9 demethylase ([histone H3]-N6,N6-dimethyl-L-lysine9,2-oxoglutarate:oxygen oxidoreductase)

enzyme lambda metabolic reaction experiment
uniprot:Q9UPP1 [Fe2+]

2 O2 + 2 2_Oxoglutarate + Histone_H3(1_24)_[trimethyl]K4_[dimethyl]K9 -> 2 CO2 + 2 Formaldehyde + 2 Succinate + Histone_H3(1_24)_[trimethyl]K4		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM HEPES, 2 mM ascorbic acid, 1 mM 3-acetylpyridine adenine dinucleotide, 0.05 U formaldehyde dehydrogenase from Escherichia coli BH249
PH: 7
Temperature: 37
uniprot:Q6ZMT4 [Fe2+]

2 O2 + 2 2_Oxoglutarate + Histone_H3(1_24)_[dimethyl]K9 -> 2 Succinate + 2 CO2 + 2 Formaldehyde + Histone_H3(1_24)		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM HEPES, 2 mM ascorbic acid, 1 mM 3-acetylpyridine adenine dinucleotide, 0.05 U formaldehyde dehydrogenase from Escherichia coli BH249
PH: 7
Temperature: 37
uniprot:Q9UPP1 [Fe2+]

Histone_H3(1_24)_[dimethyl]K9 + 2 2_Oxoglutarate + 2 O2 -> 2 CO2 + Histone_H3(1_24) + 2 Formaldehyde + 2 Succinate		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM HEPES, 2 mM ascorbic acid, 1 mM 3-acetylpyridine adenine dinucleotide, 0.05 U formaldehyde dehydrogenase from Escherichia coli BH249
PH: 7
Temperature: 37
uniprot:Q6ZMT4 [Fe2+]

2 O2 + Histone_H3(1_24)_[dimethyl]K9 + 2 2_Oxoglutarate -> 2 CO2 + 2 Formaldehyde + 2 Succinate + Histone_H3(1_24)		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM HEPES, 2 mM ascorbic acid, 1 mM 3-acetylpyridine adenine dinucleotide, 0.05 U formaldehyde dehydrogenase from Escherichia coli BH249
PH: 7
Temperature: 37

Pathways

pathway id name
Reactome:R-HSA-9679506 SARS-CoV Infections
Reactome:R-HSA-9679191 Potential therapeutics for SARS
Reactome:R-HSA-9716542 Signaling by Rho GTPases, Miro GTPases and RHOBTB3
Reactome:R-HSA-9824446 Viral Infection Pathways
Reactome:R-HSA-9759476 Regulation of Homotypic Cell-Cell Adhesion
Reactome:R-MMU-9759476 Regulation of Homotypic Cell-Cell Adhesion
Reactome:R-HSA-9764265 Regulation of CDH1 Expression and Function
Reactome:R-MMU-9764265 Regulation of CDH1 Expression and Function
Reactome:R-HSA-9764274 Regulation of Expression and Function of Type I Classical Cadherins
Reactome:R-HSA-9764725 Negative Regulation of CDH1 Gene Transcription
Reactome:R-MMU-9764274 Regulation of Expression and Function of Type I Classical Cadherins
Reactome:R-HSA-9764560 Regulation of CDH1 Gene Transcription
Reactome:R-MMU-9764560 Regulation of CDH1 Gene Transcription
Reactome:R-MMU-9764725 Negative Regulation of CDH1 Gene Transcription
Reactome:R-DRE-3247509 Chromatin modifying enzymes
Reactome:R-DRE-68886 M Phase
Reactome:R-DME-983231 Factors involved in megakaryocyte development and platelet production
Reactome:R-HSA-418990 Adherens junctions interactions
Reactome:R-HSA-1643685 Disease
Reactome:R-HSA-6802957 Oncogenic MAPK signaling
Reactome:R-HSA-3214815 HDACs deacetylate histones
Reactome:R-MMU-418990 Adherens junctions interactions
Reactome:R-MMU-162582 Signaling Pathways
Reactome:R-MMU-8939211 ESR-mediated signaling
Reactome:R-DRE-4839726 Chromatin organization
Reactome:R-DRE-3214842 HDMs demethylate histones
Reactome:R-DRE-2299718 Condensation of Prophase Chromosomes
Reactome:R-DME-109582 Hemostasis
Reactome:R-HSA-421270 Cell-cell junction organization
Reactome:R-HSA-9006931 Signaling by Nuclear Receptors
Reactome:R-HSA-9018519 Estrogen-dependent gene expression
Reactome:R-HSA-195258 RHO GTPase Effectors
Reactome:R-HSA-5625886 Activated PKN1 stimulates transcription of AR (androgen receptor) regulated genes KLK2 and KLK3
Reactome:R-MMU-983231 Factors involved in megakaryocyte development and platelet production
Reactome:R-MMU-421270 Cell-cell junction organization
Reactome:R-MMU-3247509 Chromatin modifying enzymes
Reactome:R-DRE-69278 Cell Cycle, Mitotic
Reactome:R-DRE-68875 Mitotic Prophase
Reactome:R-HSA-1500931 Cell-Cell communication
Reactome:R-HSA-109582 Hemostasis
Reactome:R-HSA-194315 Signaling by Rho GTPases
Reactome:R-HSA-5663202 Diseases of signal transduction by growth factor receptors and second messengers
Reactome:R-HSA-6802952 Signaling by BRAF and RAF1 fusions
Reactome:R-HSA-4839726 Chromatin organization
Reactome:R-HSA-3214842 HDMs demethylate histones
Reactome:R-MMU-1500931 Cell-Cell communication
Reactome:R-MMU-3214815 HDACs deacetylate histones
Reactome:R-MMU-9006931 Signaling by Nuclear Receptors
Reactome:R-MMU-9018519 Estrogen-dependent gene expression
Reactome:R-DRE-1640170 Cell Cycle
Reactome:R-HSA-446728 Cell junction organization
Reactome:R-HSA-983231 Factors involved in megakaryocyte development and platelet production
Reactome:R-HSA-162582 Signaling Pathways
Reactome:R-HSA-8939211 ESR-mediated signaling
Reactome:R-HSA-5625740 RHO GTPases activate PKNs
Reactome:R-HSA-5663205 Infectious disease
Reactome:R-HSA-3247509 Chromatin modifying enzymes
Reactome:R-MMU-109582 Hemostasis
Reactome:R-MMU-446728 Cell junction organization
Reactome:R-MMU-4839726 Chromatin organization
Reactome:R-MMU-3214842 HDMs demethylate histones
WikiPathways:WP4746 Thyroid hormones production and peripheral downstream signaling effects