EC: 6.2.1.17

propionate-CoA ligase (propanoate:CoA ligase (AMP-forming))

enzyme lambda metabolic reaction experiment
uniprot:Q8XZ86 [propionate---CoA ligase(Enzyme) wildtype]

Coenzyme_A + Acetate + ATP -> Acetyl_CoA + AMP + Diphosphate		 (( (( (E * kcat) ) * (A ^ n)) ) / (( (Km ^ n) ) + (A ^ n))) buffer: 50 mM Tris-HCl, 3 mM MgCl2, 0.5 mM dithiothreitol, 300 mM hydroxylamine
PH: 8
Temperature: 37
- [propionate---CoA ligase(Enzyme) wildtype]

Acetate + ATP + Coenzyme_A -> AMP + Acetyl_CoA + Diphosphate		 (( (( (E * kcat) ) * (A ^ n)) ) / (( (Km ^ n) ) + (A ^ n))) buffer: 50 mM sodium phosphate, 3 mM MgCl2, 300 mM hydroxylamine
PH: 7
Temperature: 37
uniprot:Q8XZ86 [propionate---CoA ligase(Enzyme) wildtype]

Coenzyme_A + Propanoate + ATP -> Propanoyl_CoA + AMP + Diphosphate		 (( (( (E * kcat) ) * (A ^ n)) ) / (( (Km ^ n) ) + (A ^ n))) buffer: 50 mM Tris-HCl, 3 mM MgCl2, 0.5 mM dithiothreitol, 300 mM hydroxylamine
PH: 8
Temperature: 37
uniprot:Q8XZ86 [propionate---CoA ligase(Enzyme) wildtype]

Butanoate + Coenzyme_A + ATP -> Diphosphate + Butanoyl_CoA + AMP		 (( (( (E * kcat) ) * (A ^ n)) ) / (( (Km ^ n) ) + (A ^ n))) buffer: 50 mM Tris-HCl, 3 mM MgCl2, 0.5 mM dithiothreitol, 300 mM hydroxylamine
PH: 8
Temperature: 37
- [propionate---CoA ligase(Enzyme) wildtype]

ATP + Coenzyme_A + Propanoate -> Propanoyl_CoA + AMP + Diphosphate		 (( (( (E * kcat) ) * (A ^ n)) ) / (( (Km ^ n) ) + (A ^ n))) buffer: 50 mM sodium phosphate, 3 mM MgCl2, 300 mM hydroxylamine
PH: 7
Temperature: 37
- [propionate---CoA ligase(Enzyme) wildtype]

Coenzyme_A + ATP + Butanoate -> Diphosphate + Butanoyl_CoA + AMP		 (( (( (E * kcat) ) * (A ^ n)) ) / (( (Km ^ n) ) + (A ^ n))) buffer: 50 mM sodium phosphate, 3 mM MgCl2, 300 mM hydroxylamine
PH: 7
Temperature: 37
uniprot:Q8XZ86 [propionate---CoA ligase(Enzyme) wildtype]

Acrylate + ATP + Coenzyme_A -> Acryloyl_CoA + AMP + Diphosphate		 (( (( (E * kcat) ) * (A ^ n)) ) / (( (Km ^ n) ) + (A ^ n))) buffer: 50 mM Tris-HCl, 3 mM MgCl2, 0.5 mM dithiothreitol, 300 mM hydroxylamine
PH: 8
Temperature: 37
- [propionate---CoA ligase(Enzyme) wildtype]

Coenzyme_A + Acrylate + ATP -> Acryloyl_CoA + AMP + Diphosphate		 (( (( (E * kcat) ) * (A ^ n)) ) / (( (Km ^ n) ) + (A ^ n))) buffer: 50 mM sodium phosphate, 3 mM MgCl2, 300 mM hydroxylamine
PH: 7
Temperature: 37
- [propionate---CoA ligase(Enzyme) wildtype]

Propanoate + Coenzyme_A + ATP -> Diphosphate + AMP + Propanoyl_CoA		 (( (Vmax * S) ) / (Km + S)) buffer: 0.05 M Tris, 16.7 mM Mg2+
PH: 8.6
Temperature: 37
- [propionate---CoA ligase(Enzyme) wildtype]

Propanoate + ATP + Coenzyme_A -> AMP + Diphosphate + Propanoyl_CoA		 (( (Vmax * S) ) / (Km + S)) buffer: 0.05 M Tris, 16.7 mM Mg2+
PH: 8.6
Temperature: 37
- [propionate---CoA ligase(Enzyme) wildtype]

ATP + Coenzyme_A + Propanoate -> Diphosphate + AMP + Propanoyl_CoA		 (( (Vmax * S) ) / (Km + S)) buffer: 0.05 M Tris, 16.7 mM Mg2+
PH: 8.6
Temperature: 37

Pathways

pathway id name
BioCyc:HUMAN_PWY0-1313 acetate conversion to acetyl-CoA
BioCyc:HUMAN_PWY66-161 oxidative ethanol degradation III
BioCyc:HUMAN_PWY-7592 arachidonate biosynthesis III (metazoa)
BioCyc:META_PWY66-21 ethanol degradation II
BioCyc:META_PWY66-161 ethanol degradation III
BioCyc:META_PWY66-162 ethanol degradation IV
BioCyc:META_PWY-7857 adlupulone and adhumulone biosynthesis
BioCyc:HUMAN_PWY66-162 ethanol degradation IV
BioCyc:HUMAN_PWY66-21 ethanol degradation II
BioCyc:META_PWY0-42 2-methylcitrate cycle I
BioCyc:ECO_PWY0-42 2-methylcitrate cycle I
BioCyc:MOUSE_PWY66-21 ethanol degradation II (cytosol)
BioCyc:HUMAN_PWY-7049 icosapentaenoate biosynthesis II (metazoa)
BioCyc:HUMAN_PWY-6000 γ-linolenate biosynthesis
BioCyc:META_PWY0-1313 acetate conversion to acetyl-CoA
BioCyc:META_PWY-5133 colupulone and cohumulone biosynthesis
BioCyc:ECOL316407_PWY0-42 2-methylcitrate cycle I
BioCyc:META_PWY-5789 3-hydroxypropanoate/4-hydroxybutanate cycle
BioCyc:META_PWY-5134 superpathway of bitter acids biosynthesis
BioCyc:MOUSE_PWY0-1313 acetate conversion to acetyl-CoA
PathBank:SMP0000957 Propanoate Metabolism
PlantCyc:HOP_PWY-7857 adlupulone and adhumulone biosynthesis
PlantCyc:HOP_PWY-5134 superpathway of bitter acids biosynthesis
PlantCyc:HOP_PWY-5133 colupulone and cohumulone biosynthesis
PlantCyc:PLANT_PWY-7857 adlupulone and adhumulone biosynthesis
PlantCyc:PLANT_PWY-5134 superpathway of bitter acids biosynthesis
PlantCyc:PLANT_PWY-5133 colupulone and cohumulone biosynthesis
Reactome:R-HSA-9818564 Epigenetic regulation of gene expression by MLL3 and MLL4 complexes
Reactome:R-HSA-9917777 Epigenetic regulation by WDR5-containing histone modifying complexes
Reactome:R-HSA-9851695 Epigenetic regulation of adipogenesis genes by MLL3 and MLL4 complexes
Reactome:R-HSA-9841922 MLL4 and MLL3 complexes regulate expression of PPARG target genes in adipogenesis and hepatic steatosis
Reactome:R-BTA-1430728 Metabolism
Reactome:R-BTA-556833 Metabolism of lipids
Reactome:R-HSA-211859 Biological oxidations
Reactome:R-HSA-74160 Gene expression (Transcription)
Reactome:R-MMU-1430728 Metabolism
Reactome:R-MMU-556833 Metabolism of lipids
Reactome:R-MMU-77111 Synthesis of Ketone Bodies
Reactome:R-MMU-211859 Biological oxidations
Reactome:R-RNO-74182 Ketone body metabolism
Reactome:R-BTA-77111 Synthesis of Ketone Bodies
Reactome:R-HSA-556833 Metabolism of lipids
Reactome:R-HSA-77111 Synthesis of Ketone Bodies
Reactome:R-HSA-212165 Epigenetic regulation of gene expression
Reactome:R-MMU-74182 Ketone body metabolism
Reactome:R-HSA-211945 Phase I - Functionalization of compounds
Reactome:R-HSA-71384 Ethanol oxidation
Reactome:R-MMU-211945 Phase I - Functionalization of compounds
Reactome:R-MMU-71384 Ethanol oxidation
Reactome:R-RNO-1430728 Metabolism
Reactome:R-RNO-556833 Metabolism of lipids
Reactome:R-RNO-77111 Synthesis of Ketone Bodies
Reactome:R-BTA-74182 Ketone body metabolism
Reactome:R-HSA-1430728 Metabolism
Reactome:R-HSA-74182 Ketone body metabolism
PathBank:SMP0000016 Propanoate Metabolism
PathBank:SMP0000334 Pyruvate Decarboxylase E1 Component Deficiency (PDHE1 Deficiency)
PathBank:SMP0000568 Short-Chain 3-Hydroxyacyl-CoA Dehydrogenase Deficiency (SCHAD)
PathBank:SMP0000196 Leigh Syndrome
PathBank:SMP0000201 Methylmalonic Aciduria Due to Cobalamin-Related Disorders
PathBank:SMP0000429 Disulfiram Action Pathway
PathBank:SMP0000559 Pyruvate Kinase Deficiency
PathBank:SMP0087265 Mitochondrial Beta-Oxidation of Short Chain Saturated Fatty Acids
PathBank:SMP0000212 Pyruvate Dehydrogenase Complex Deficiency
PathBank:SMP0087248 Propanoate Metabolism
PathBank:SMP0000060 Pyruvate Metabolism
PathBank:SMP0000449 Ethanol Degradation
PathBank:SMP0000480 Mitochondrial Beta-Oxidation of Short Chain Saturated Fatty Acids
PathBank:SMP0000198 Malonic Aciduria
PathBank:SMP0000502 Malonyl-CoA Decarboxylase Deficiency
PathBank:SMP0000558 Primary Hyperoxaluria II, PH2