3-hydroxypropanoate/4-hydroxybutanate cycle

Berg IA, Kockelkorn D, Buckel W, Fuchs G. A 3-hydroxypropionate/4-hydroxybutyrate autotrophic carbon dioxide assimilation pathway in Archaea. Science. 2007 Dec 14;318(5857):1782–6. doi: 10.1126/science.1149976. PMID: 18079405.; Zhu X, Lapthorn AJ, Ellis EM. Crystal structure of mouse succinic semialdehyde reductase AKR7A5: structural basis for substrate specificity. Biochemistry. 2006 Feb 14;45(6):1562–70. doi: 10.1021/bi051610k. PMID: 16460003.; Murakami H, Sly WS. Purification and characterization of human salivary carbonic anhydrase. Journal of Biological Chemistry. 1987 Jan;262(3):1382–8. doi: 10.1016/s0021-9258(19)75797-4.

Metabolites

CO2

Formula: CO2 (43.98983)

CAS ID: 124-38-9

Cobalt ion

Formula: Co (58.933198)

CAS ID: 22541-53-3

Diphosphate

Formula: H4P2O7 (177.9432294)

CAS ID: 2466-09-3

Dithioerythritol

Formula: C4H10O2S2 (154.01222)

CAS ID: 6892-68-8

H+

Formula: H (1.0078246)

CAS ID: 12408-02-5

H2O

Formula: H2O (18.0105642)

CAS ID: 7732-18-5

HCO3-

Formula: HCO3 (60.9925696)

CAS ID: 71-52-3

Magnesium cation

Formula: Mg (23.98505)

CAS ID: 22537-22-0

Nickel(2+)

Formula: Ni (57.935347)

CAS ID: 14701-22-5

Zinc cation

Formula: Zn (63.929145)

CAS ID: 23713-49-7

iodoacetamide

Formula: C2H4INO (184.9337644)

CAS ID: 144-48-9

4-hydroxybutyrate

Formula: C4H7O3 (103.0395172)

CAS ID: 1320-61-2

biotinate

Formula: C10H15N2O3S (243.080334)

CAS ID:

NADPH(4-)

Formula: C21H26N7O17P3 (741.0598016)

CAS ID: 53-57-6

ATP(4-)

Formula: C10H12N5O13P3 (502.9644492)

CAS ID:

NAD(1-)

Formula: C21H26N7O14P2 (662.1012936000001)

CAS ID: 53-84-9

ADP(3-)

Formula: C10H12N5O10P2 (424.0059412)

CAS ID:

NADP(3-)

Formula: C21H25N7O17P3 (740.051977)

CAS ID: 53-59-8



Enzyme

EC Number name full name note
1.2.1.75 malonyl-CoA reductase (malonate semialdehyde-forming) malonate semialdehyde:NADP+ oxidoreductase (malonate semialdehyde-forming)
1.2.1.76 succinate-semialdehyde dehydrogenase (acylating) succinate semialdehyde:NADP+ oxidoreductase (CoA-acylating)
1.3.1.84 acrylyl-CoA reductase (NADPH) propanoyl-CoA:NADP+ oxidoreductase
4.2.1.116 3-hydroxypropionyl-CoA dehydratase 3-hydroxypropionyl-CoA hydro-lyase
6.2.1.1 acetate-CoA ligase acetate:CoA ligase (AMP-forming)
6.2.1.17 propionate-CoA ligase propanoate:CoA ligase (AMP-forming)
6.2.1.2 medium-chain acyl-CoA ligase medium-chain fatty acid:CoA ligase (AMP-forming)
6.2.1.36 3-hydroxypropionyl-CoA synthase 3-hydroxypropionate:CoA ligase (AMP-forming)
6.2.1.40 4-hydroxybutyrate-CoA ligase (AMP-forming) 4-hydroxybutyrate:CoA ligase (AMP formimg)


Proteins

Protein ID name full name
A4YDT1 Msed_0406 AMP-dependent synthetase and ligase
A4YEN2 Msed_0709 succinyl-CoA reductase (NADPH)
A4YGN0 Msed_1424 succinate semialdehyde reductase (NADPH)
A4YGR1 Msed_1456 3-hydroxypropionyl-CoA synthetase
A4YI89 Msed_2001 short chain enoyl-CoA hydratase
Q96YK1 mcr Malonyl-CoA reductase
Q975C8 ACAR_SULTO Acryloyl-coenzyme A reductase