EC: 3.4.21.89

Signal peptidase I ()

enzyme lambda metabolic reaction experiment
uniprot:Q5JE91 [signal peptidase I(Enzyme) mutant E226A]

H2O + Ala_Ala_Phe_7_Amino_4_methylcoumarin -> Ala_Ala_Phe + 7_Amino_4_methylcoumarin		 (( (Vmax * S) ) / (( (Ks1 + S) ) + (( (1 / Ks2) ) * (S ^ 2)))) buffer: 50 mM CHES, 3% Me2SO
PH: 10
Temperature: 60
uniprot:Q5JE91 [signal peptidase I(Enzyme) mutant K209A]

H2O + Ala_Ala_Phe_7_Amino_4_methylcoumarin -> Ala_Ala_Phe + 7_Amino_4_methylcoumarin		 (( (Vmax * S) ) / (( (Ks1 + S) ) + (( (1 / Ks2) ) * (S ^ 2)))) buffer: 50 mM CHES, 3% Me2SO
PH: 10
Temperature: 60
uniprot:Q5JE91 [signal peptidase I(Enzyme) mutant R250A]

Ala_Ala_Phe_7_Amino_4_methylcoumarin + H2O -> 7_Amino_4_methylcoumarin + Ala_Ala_Phe		 (( (Vmax * S) ) / (( (Ks1 + S) ) + (( (1 / Ks2) ) * (S ^ 2)))) buffer: 50 mM CHES, 3% Me2SO
PH: 10
Temperature: 60
uniprot:Q5JE91 [signal peptidase I(Enzyme) mutant D215A]

H2O + Ala_Ala_Phe_7_Amino_4_methylcoumarin -> 7_Amino_4_methylcoumarin + Ala_Ala_Phe		 (( (Vmax * S) ) / (( (Ks1 + S) ) + (( (1 / Ks2) ) * (S ^ 2)))) buffer: 50 mM CHES, 3% Me2SO
PH: 10
Temperature: 60
uniprot:Q5JE91 [signal peptidase I(Enzyme) mutant E227A]

H2O + Ala_Ala_Phe_7_Amino_4_methylcoumarin -> Ala_Ala_Phe + 7_Amino_4_methylcoumarin		 (( (Vmax * S) ) / (( (Ks1 + S) ) + (( (1 / Ks2) ) * (S ^ 2)))) buffer: 50 mM CHES, 3% Me2SO
PH: 10
Temperature: 60
uniprot:Q5JE91 [signal peptidase I(Enzyme) mutant R221A]

H2O + Ala_Ala_Phe_7_Amino_4_methylcoumarin -> 7_Amino_4_methylcoumarin + Ala_Ala_Phe		 (( (Vmax * S) ) / (( (Ks1 + S) ) + (( (1 / Ks2) ) * (S ^ 2)))) buffer: 50 mM CHES, 3% Me2SO
PH: 10
Temperature: 60
uniprot:Q5JE91 [signal peptidase I(Enzyme) mutant D277A]

Ala_Ala_Phe_7_Amino_4_methylcoumarin + H2O -> Ala_Ala_Phe + 7_Amino_4_methylcoumarin		 (( (Vmax * S) ) / (( (Ks1 + S) ) + (( (1 / Ks2) ) * (S ^ 2)))) buffer: 50 mM CHES, 3% Me2SO
PH: 10
Temperature: 60
uniprot:Q5JE91 [signal peptidase I(Enzyme) mutant S128A]

H2O + Ala_Ala_Phe_7_Amino_4_methylcoumarin -> Ala_Ala_Phe + 7_Amino_4_methylcoumarin		 (( (Vmax * S) ) / (( (Ks1 + S) ) + (( (1 / Ks2) ) * (S ^ 2)))) buffer: 50 mM CHES, 3% Me2SO
PH: 10
Temperature: 60
uniprot:Q5JE91 [signal peptidase I(Enzyme) mutant S184A]

H2O + Ala_Ala_Phe_7_Amino_4_methylcoumarin -> 7_Amino_4_methylcoumarin + Ala_Ala_Phe		 (( (Vmax * S) ) / (( (Ks1 + S) ) + (( (1 / Ks2) ) * (S ^ 2)))) buffer: 50 mM CHES, 3% Me2SO
PH: 10
Temperature: 60
uniprot:Q5JE91 [signal peptidase I(Enzyme) mutant H191A]

H2O + Ala_Ala_Phe_7_Amino_4_methylcoumarin -> Ala_Ala_Phe + 7_Amino_4_methylcoumarin		 (( (Vmax * S) ) / (( (Ks1 + S) ) + (( (1 / Ks2) ) * (S ^ 2)))) buffer: 50 mM CHES, 3% Me2SO
PH: 10
Temperature: 60
uniprot:Q5JE91 [signal peptidase I(Enzyme) mutant Y165A]

H2O + Ala_Ala_Phe_7_Amino_4_methylcoumarin -> 7_Amino_4_methylcoumarin + Ala_Ala_Phe		 (( (Vmax * S) ) / (( (Ks1 + S) ) + (( (1 / Ks2) ) * (S ^ 2)))) buffer: 50 mM CHES, 3% Me2SO
PH: 10
Temperature: 60
uniprot:Q5JE91 [signal peptidase I(Enzyme) wildtype]

Ala_Ala_Phe_7_Amino_4_methylcoumarin + H2O -> Ala_Ala_Phe + 7_Amino_4_methylcoumarin		 (( (Vmax * S) ) / (( (Ks1 + S) ) + (( (1 / Ks2) ) * (S ^ 2)))) buffer: 50 mM CHES, 3% Me2SO
PH: 10
Temperature: 60
uniprot:Q5JE91 [signal peptidase I(Enzyme) mutant K150A]

Ala_Ala_Phe_7_Amino_4_methylcoumarin + H2O -> 7_Amino_4_methylcoumarin + Ala_Ala_Phe		 (( (Vmax * S) ) / (( (Ks1 + S) ) + (( (1 / Ks2) ) * (S ^ 2)))) buffer: 50 mM CHES, 3% Me2SO
PH: 10
Temperature: 60
uniprot:Q5JE91 [signal peptidase I(Enzyme) mutant S130A]

Ala_Ala_Phe_7_Amino_4_methylcoumarin + H2O -> Ala_Ala_Phe + 7_Amino_4_methylcoumarin		 (( (Vmax * S) ) / (( (Ks1 + S) ) + (( (1 / Ks2) ) * (S ^ 2)))) buffer: 50 mM CHES, 3% Me2SO
PH: 10
Temperature: 60
uniprot:Q5JE91 [signal peptidase I(Enzyme) mutant S131A]

Ala_Ala_Phe_7_Amino_4_methylcoumarin + H2O -> 7_Amino_4_methylcoumarin + Ala_Ala_Phe		 (( (Vmax * S) ) / (( (Ks1 + S) ) + (( (1 / Ks2) ) * (S ^ 2)))) buffer: 50 mM CHES, 3% Me2SO
PH: 10
Temperature: 60

Pathways

pathway id name
Reactome:R-HSA-9824439 Bacterial Infection Pathways
Reactome:R-HSA-9820965 Respiratory syncytial virus (RSV) genome replication, transcription and translation
Reactome:R-HSA-9828806 Maturation of hRSV A proteins
Reactome:R-HSA-9824446 Viral Infection Pathways
Reactome:R-BTA-9759476 Regulation of Homotypic Cell-Cell Adhesion
Reactome:R-HSA-9759476 Regulation of Homotypic Cell-Cell Adhesion
Reactome:R-RNO-9759476 Regulation of Homotypic Cell-Cell Adhesion
Reactome:R-CFA-9759476 Regulation of Homotypic Cell-Cell Adhesion
Reactome:R-MMU-9759476 Regulation of Homotypic Cell-Cell Adhesion
Reactome:R-HSA-9820952 Respiratory Syncytial Virus Infection Pathway
Reactome:R-CFA-9764274 Regulation of Expression and Function of Type I Classical Cadherins
Reactome:R-CFA-9768727 Regulation of CDH1 posttranslational processing and trafficking to plasma membrane
Reactome:R-HSA-9764265 Regulation of CDH1 Expression and Function
Reactome:R-HSA-9760173 Secretion of toxins
Reactome:R-MMU-9764265 Regulation of CDH1 Expression and Function
Reactome:R-HSA-9918432 Maturation of DENV proteins
Reactome:R-HSA-9764274 Regulation of Expression and Function of Type I Classical Cadherins
Reactome:R-HSA-9640148 Infection with Enterobacteria
Reactome:R-MMU-9764274 Regulation of Expression and Function of Type I Classical Cadherins
Reactome:R-MMU-9768727 Regulation of CDH1 posttranslational processing and trafficking to plasma membrane
Reactome:R-HSA-9918487 Dengue Virus Genome Translation and Replication
Reactome:R-BTA-9764274 Regulation of Expression and Function of Type I Classical Cadherins
Reactome:R-BTA-9768727 Regulation of CDH1 posttranslational processing and trafficking to plasma membrane
Reactome:R-CFA-9764265 Regulation of CDH1 Expression and Function
Reactome:R-HSA-9768727 Regulation of CDH1 posttranslational processing and trafficking to plasma membrane
Reactome:R-RNO-9764274 Regulation of Expression and Function of Type I Classical Cadherins
Reactome:R-RNO-9768727 Regulation of CDH1 posttranslational processing and trafficking to plasma membrane
Reactome:R-HSA-9839923 Dengue Virus Infection
Reactome:R-BTA-9764265 Regulation of CDH1 Expression and Function
Reactome:R-RNO-9764265 Regulation of CDH1 Expression and Function
Reactome:R-BTA-1500931 Cell-Cell communication
Reactome:R-CFA-421270 Cell-cell junction organization
Reactome:R-HSA-418990 Adherens junctions interactions
Reactome:R-HSA-392499 Metabolism of proteins
Reactome:R-HSA-381771 Synthesis, secretion, and inactivation of Glucagon-like Peptide-1 (GLP-1)
Reactome:R-HSA-1643685 Disease
Reactome:R-MMU-418990 Adherens junctions interactions
Reactome:R-MMU-2980736 Peptide hormone metabolism
Reactome:R-RNO-421270 Cell-cell junction organization
Reactome:R-BTA-2980736 Peptide hormone metabolism
Reactome:R-BTA-418990 Adherens junctions interactions
Reactome:R-CFA-446728 Cell junction organization
Reactome:R-CFA-2980736 Peptide hormone metabolism
Reactome:R-HSA-421270 Cell-cell junction organization
Reactome:R-HSA-1799339 SRP-dependent cotranslational protein targeting to membrane
Reactome:R-HSA-2980736 Peptide hormone metabolism
Reactome:R-HSA-400508 Incretin synthesis, secretion, and inactivation
Reactome:R-MMU-421270 Cell-cell junction organization
Reactome:R-RNO-392499 Metabolism of proteins
Reactome:R-RNO-2980736 Peptide hormone metabolism
Reactome:R-RNO-446728 Cell junction organization
Reactome:R-BTA-392499 Metabolism of proteins
Reactome:R-BTA-422085 Synthesis, secretion, and deacylation of Ghrelin
Reactome:R-BTA-446728 Cell junction organization
Reactome:R-CFA-418990 Adherens junctions interactions
Reactome:R-CFA-392499 Metabolism of proteins
Reactome:R-CFA-422085 Synthesis, secretion, and deacylation of Ghrelin
Reactome:R-HSA-1500931 Cell-Cell communication
Reactome:R-HSA-72766 Translation
Reactome:R-HSA-422085 Synthesis, secretion, and deacylation of Ghrelin
Reactome:R-HSA-400511 Synthesis, secretion, and inactivation of Glucose-dependent Insulinotropic Polypeptide (GIP)
Reactome:R-MMU-1500931 Cell-Cell communication
Reactome:R-MMU-392499 Metabolism of proteins
Reactome:R-RNO-422085 Synthesis, secretion, and deacylation of Ghrelin
Reactome:R-RNO-418990 Adherens junctions interactions
Reactome:R-BTA-421270 Cell-cell junction organization
Reactome:R-CFA-1500931 Cell-Cell communication
Reactome:R-HSA-446728 Cell junction organization
Reactome:R-HSA-5663205 Infectious disease
Reactome:R-MMU-446728 Cell junction organization
Reactome:R-MMU-422085 Synthesis, secretion, and deacylation of Ghrelin
Reactome:R-RNO-1500931 Cell-Cell communication