EC: 3.2.2.21

DNA-3-methyladenine glycosylase II (alkylated-DNA glycohydrolase (releasing methyladenine and methylguanine))

enzyme lambda metabolic reaction experiment
uniprot:P29372 [DNA-3-methyladenine glycosylase II(Enzyme) wildtype]

H2O + Hypoxanthine_containing_DNA -> Inosine + DNA		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 70 mM MOPS, 1 mM Dithiothreitol, 1 mM EDTA, 5% Glycerol
PH: 7.5
Temperature: 37
uniprot:P94378 [DNA-3-methyladenine glycosylase II(Enzyme) wildtype]

Hypoxanthine_containing_DNA + H2O -> Inosine + DNA		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 70 mM MOPS, 1 mM Dithiothreitol, 1 mM EDTA, 5% Glycerol
PH: 7.5
Temperature: 37
uniprot:P29372 [DNA-3-methyladenine glycosylase II(Enzyme) wildtype]

1,N6_ethenoadenine_containing_DNA + H2O -> DNA + 1,N6_ethenoadenine		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 70 mM MOPS, 1 mM Dithiothreitol, 1 mM EDTA, 5% Glycerol
PH: 7.5
Temperature: 37
uniprot:P94378 [DNA-3-methyladenine glycosylase II(Enzyme) wildtype]

H2O + 1,N6_ethenoadenine_containing_DNA -> DNA + 1,N6_ethenoadenine		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 70 mM MOPS, 1 mM Dithiothreitol, 1 mM EDTA, 5% glycerol
PH: 7.5
Temperature: 37

Pathways

pathway id name
Reactome:R-HSA-73929 Base-Excision Repair, AP Site Formation
Reactome:R-HSA-110357 Displacement of DNA glycosylase by APEX1
Reactome:R-MMU-73927 Depurination
Reactome:R-MMU-73933 Resolution of Abasic Sites (AP sites)
Reactome:R-RNO-73894 DNA Repair
Reactome:R-RNO-110331 Cleavage of the damaged purine
Reactome:R-RNO-110357 Displacement of DNA glycosylase by APEX1
Reactome:R-HSA-73884 Base Excision Repair
Reactome:R-HSA-110331 Cleavage of the damaged purine
Reactome:R-HSA-73933 Resolution of Abasic Sites (AP sites)
Reactome:R-MMU-73929 Base-Excision Repair, AP Site Formation
Reactome:R-RNO-73927 Depurination
Reactome:R-RNO-73933 Resolution of Abasic Sites (AP sites)
Reactome:R-HSA-73927 Depurination
Reactome:R-MMU-73894 DNA Repair
Reactome:R-MMU-110331 Cleavage of the damaged purine
Reactome:R-MMU-110357 Displacement of DNA glycosylase by APEX1
Reactome:R-RNO-73884 Base Excision Repair
Reactome:R-HSA-73894 DNA Repair
Reactome:R-HSA-110330 Recognition and association of DNA glycosylase with site containing an affected purine
Reactome:R-MMU-73884 Base Excision Repair
Reactome:R-RNO-73929 Base-Excision Repair, AP Site Formation