EC: 3.1.3.36

phosphoinositide 5-phosphatase (phosphatidyl-myo-inositol-4,5-bisphosphate 4-phosphohydrolase)

enzyme lambda metabolic reaction experiment
uniprot:O43001 [Mn2+]

H2O + D_myo_Inositol_1,4,5_trisphosphate -> 1D_myo_Inositol_1,4_bisphosphate + Phosphate		 (( (E * kcat) ) / (( (1 + (Km / C)) ) + (C / Ki))) buffer: 50 mM Hepes, 100 mM NaCl, 0.25 mM EDTA
PH: 7.4
Temperature: 30
uniprot:O43001 [Mg2+]

H2O + D_myo_Inositol_1,4,5_trisphosphate -> 1D_myo_Inositol_1,4_bisphosphate + Phosphate		 (( (E * kcat) ) / (( (1 + (Km / C)) ) + (C / Ki))) buffer: 50 mM Hepes, 100 mM NaCl, 0.25 mM EDTA
PH: 7.4
Temperature: 30
uniprot:O43001 [Co2+]

H2O + D_myo_Inositol_1,4,5_trisphosphate -> 1D_myo_Inositol_1,4_bisphosphate + Phosphate		 (( (E * kcat) ) / (( (1 + (Km / C)) ) + (C / Ki))) buffer: 50 mM Hepes, 100 mM NaCl, 0.25 mM EDTA
PH: 7.4
Temperature: 30
uniprot:O43001 [Mg2+]

D_myo_Inositol_1,4,5_trisphosphate + H2O -> Phosphate + 1D_myo_Inositol_1,4_bisphosphate		 (( (( (E * kcat) ) * C) ) / (Km + C)) buffer: 50 mM Hepes, 100 mM NaCl, 0.25 mM EDTA
PH: 7.4
Temperature: 30
uniprot:O43001 [Ni2+]

H2O + D_myo_Inositol_1,4,5_trisphosphate -> Phosphate + 1D_myo_Inositol_1,4_bisphosphate		 (( (E * kcat) ) / (( (1 + (Km / C)) ) + (C / Ki))) buffer: 50 mM Hepes, 100 mM NaCl, 0.25 mM EDTA
PH: 7.4
Temperature: 30
- [Mg2+]

1_Phosphatidyl_D_myo_inositol_4,5_bisphosphate + H2O -> 1_Phosphatidyl_1D_myo_inositol_4_phosphate + Phosphate		 (( (Vmax * A) ) / (Km + A)) buffer: 45 mM Tris-HCl, 0.72 mM Cetyltrimethylammonium bromide
PH: 7.2
Temperature: 37
- [Mg2+]

1_Phosphatidyl_D_myo_inositol_4,5_bisphosphate + H2O -> Phosphate + 1_Phosphatidyl_1D_myo_inositol_4_phosphate		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Hepes, 0.2% (w/v) Triton X-100, 0.1 mM EGTA, 2.5 mM CTAB
PH: 7.2
Temperature: 37
- [Mg2+]

H2O + 1_Phosphatidyl_D_myo_inositol_4,5_bisphosphate -> 1_Phosphatidyl_1D_myo_inositol_4_phosphate + Phosphate		 (( (Vmax * B) ) / (Km + B)) buffer: 50 mM Hepes, 0.2% (w/v) Triton X-100, 0.1 mM EGTA, 2.5 mM CTAB
PH: 7.2
Temperature: 37
uniprot:P32019 [phosphoinositide 5-phosphatase(Enzyme) wildtype]

1_Phosphatidyl_D_myo_inositol_4,5_bisphosphate + H2O -> 1_Phosphatidyl_1D_myo_inositol_4_phosphate + Phosphate		 (( (V * S) ) / (Km + S)) buffer: 20 mM Tris, 150 mM NaCl, 200 µg/ml bovine serum albumin, 3 mM MgCl2, 2 mM cetyltriethylammonium bromide
PH: 7.2
Temperature: 37
uniprot:P32019 [phosphoinositide 5-phosphatase(Enzyme) wildtype]

H2O + 1_Phosphatidyl_D_myo_inositol_4,5_bisphosphate -> 1_Phosphatidyl_1D_myo_inositol_4_phosphate + Phosphate		 (( (V * S) ) / (Km + S)) buffer: 20 mM Tris, 150 mM NaCl, 200 µg/ml bovine serum albumin, 3 mM MgCl2, 2 mM cetyltriethylammonium bromide
PH: 7.2
Temperature: 37
uniprot:P32019 [phosphoinositide 5-phosphatase(Enzyme) wildtype]

H2O + 1_Phosphatidyl_D_myo_inositol_4,5_bisphosphate -> 1_Phosphatidyl_1D_myo_inositol_4_phosphate + Phosphate		 (( (V * S) ) / (Km + S)) buffer: 20 mM Tris, 150 mM NaCl, 200 µg/ml bovine serum albumin, 3 mM MgCl2, 2 mM cetyltriethylammonium bromide
PH: 7.2
Temperature: 37
uniprot:P32019 [phosphoinositide 5-phosphatase(Enzyme) wildtype]

1_Phosphatidyl_D_myo_inositol_4,5_bisphosphate + H2O -> Phosphate + 1_Phosphatidyl_1D_myo_inositol_4_phosphate		 (( (V * S) ) / (Km + S)) buffer: 20 mM Tris, 150 mM NaCl, 200 µg/ml bovine serum albumin, 3 mM MgCl2, 2 mM cetyltriethylammonium bromide
PH: 7.2
Temperature: 37
uniprot:Q9SKB7 [Mg2+]

H2O + 1_Phosphatidyl_D_myo_inositol_4,5_bisphosphate -> Phosphate + 1_Phosphatidyl_1D_myo_inositol_4_phosphate		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Tris-HCl, 0.25% beta-D-octylglucoside, 1 mM PMSF
PH: 7
Temperature: 30

Pathways

pathway id name
BioCyc:HUMAN_PWY-6352 3-phosphoinositide biosynthesis
BioCyc:META_PWY-6352 3-phosphoinositide biosynthesis
BioCyc:META_PWY-6358 superpathway of D-myo-inositol (1,4,5)-trisphosphate metabolism
BioCyc:ARA_PWY-6363 D-myo-inositol (1,4,5)-trisphosphate degradation
BioCyc:ARA_PWY-6352 3-phosphoinositide biosynthesis
BioCyc:MOUSE_PWY3DJ-219 PIP metabolism
BioCyc:HUMAN_PWY-6364 D-myo-inositol (1,3,4)-trisphosphate biosynthesis
BioCyc:META_PWY-6362 1D-myo-inositol hexakisphosphate biosynthesis II (mammalian)
BioCyc:META_PWY-6368 3-phosphoinositide degradation
BioCyc:META_PWY-6363 D-myo-inositol (1,4,5)-trisphosphate degradation
BioCyc:META_PWY-6364 D-myo-inositol (1,3,4)-trisphosphate biosynthesis
BioCyc:ARA_PWY-6364 D-myo-inositol (1,3,4)-trisphosphate biosynthesis
BioCyc:MOUSE_PWY-6352 3-phosphoinositide biosynthesis
BioCyc:MOUSE_PWY-6358 superpathway of D-myo-inositol (1,4,5)-trisphosphate metabolism
BioCyc:HUMAN_PWY-6368 3-phosphoinositide degradation
BioCyc:HUMAN_PWY-6362 1D-myo-inositol hexakisphosphate biosynthesis II (mammalian)
BioCyc:HUMAN_PWY-6363 D-myo-inositol (1,4,5)-trisphosphate degradation
BioCyc:HUMAN_PWY-6358 superpathway of D-myo-inositol (1,4,5)-trisphosphate metabolism
BioCyc:YEAST_PWY-6352 3-phosphoinositide biosynthesis
BioCyc:MOUSE_PWY-6363 D-myo-inositol (1,4,5)-trisphosphate degradation
BioCyc:HUMAN_PWY-6371 superpathway of inositol phosphate compounds
BioCyc:YEAST_PWY-6364 D-myo-inositol (1,3,4)-trisphosphate biosynthesis
BioCyc:YEAST_PWY3O-242 phosphatidylinositol phosphate biosynthesis
BioCyc:MOUSE_PWY-6362 1D-myo-inositol hexakisphosphate biosynthesis II (mammalian)
BioCyc:MOUSE_PWY-6364 D-myo-inositol (1,3,4)-trisphosphate biosynthesis
PlantCyc:ARA_PWY-6352 3-phosphoinositide biosynthesis
PlantCyc:ARA_PWY-6363 D-myo-inositol (1,4,5)-trisphosphate degradation
PlantCyc:PLANT_PWY-6363 D-myo-inositol (1,4,5)-trisphosphate degradation
PlantCyc:ARA_PWY-6364 D-myo-inositol (1,3,4)-trisphosphate biosynthesis
Reactome:R-HSA-9680350 Signaling by CSF1 (M-CSF) in myeloid cells
Reactome:R-RNO-210990 PECAM1 interactions
Reactome:R-HSA-210990 PECAM1 interactions
Reactome:R-MMU-168256 Immune System
Reactome:R-MMU-1430728 Metabolism
Reactome:R-MMU-556833 Metabolism of lipids
Reactome:R-MMU-1483255 PI Metabolism
Reactome:R-MMU-1660516 Synthesis of PIPs at the early endosome membrane
Reactome:R-RNO-5624958 ARL13B-mediated ciliary trafficking of INPP5E
Reactome:R-RNO-109582 Hemostasis
Reactome:R-RNO-202733 Cell surface interactions at the vascular wall
Reactome:R-RNO-1280218 Adaptive Immune System
Reactome:R-SCE-1660499 Synthesis of PIPs at the plasma membrane
Reactome:R-HSA-5617833 Cilium Assembly
Reactome:R-HSA-556833 Metabolism of lipids
Reactome:R-HSA-1660514 Synthesis of PIPs at the Golgi membrane
Reactome:R-HSA-1660516 Synthesis of PIPs at the early endosome membrane
Reactome:R-HSA-202403 TCR signaling
Reactome:R-HSA-202424 Downstream TCR signaling
Reactome:R-MMU-202733 Cell surface interactions at the vascular wall
Reactome:R-MMU-5620920 Cargo trafficking to the periciliary membrane
Reactome:R-MMU-5624958 ARL13B-mediated ciliary trafficking of INPP5E
Reactome:R-RNO-1852241 Organelle biogenesis and maintenance
Reactome:R-RNO-5617833 Cilium Assembly
Reactome:R-RNO-168256 Immune System
Reactome:R-SCE-1430728 Metabolism
Reactome:R-HSA-5620920 Cargo trafficking to the periciliary membrane
Reactome:R-HSA-5624958 ARL13B-mediated ciliary trafficking of INPP5E
Reactome:R-HSA-109582 Hemostasis
Reactome:R-HSA-1483257 Phospholipid metabolism
Reactome:R-HSA-1483255 PI Metabolism
Reactome:R-HSA-168256 Immune System
Reactome:R-MMU-1280218 Adaptive Immune System
Reactome:R-MMU-1483257 Phospholipid metabolism
Reactome:R-MMU-1660517 Synthesis of PIPs at the late endosome membrane
Reactome:R-MMU-210990 PECAM1 interactions
Reactome:R-MMU-1852241 Organelle biogenesis and maintenance
Reactome:R-MMU-5617833 Cilium Assembly
Reactome:R-RNO-5620920 Cargo trafficking to the periciliary membrane
Reactome:R-RNO-202403 TCR signaling
Reactome:R-RNO-202424 Downstream TCR signaling
Reactome:R-SCE-556833 Metabolism of lipids
Reactome:R-SCE-1483255 PI Metabolism
Reactome:R-HSA-1852241 Organelle biogenesis and maintenance
Reactome:R-HSA-202733 Cell surface interactions at the vascular wall
Reactome:R-HSA-1430728 Metabolism
Reactome:R-HSA-1660517 Synthesis of PIPs at the late endosome membrane
Reactome:R-HSA-1280218 Adaptive Immune System
Reactome:R-HSA-1280215 Cytokine Signaling in Immune system
Reactome:R-MMU-202403 TCR signaling
Reactome:R-MMU-202424 Downstream TCR signaling
Reactome:R-MMU-1660514 Synthesis of PIPs at the Golgi membrane
Reactome:R-MMU-109582 Hemostasis
Reactome:R-SCE-1483257 Phospholipid metabolism
WikiPathways:WP4971 Phosphoinositides metabolism
WikiPathways:WP5199 PtdIns(4,5)P2 in cytokinesis pathway
PathBank:SMP0002397 Inositol Metabolism
PathBank:SMP0087242 Inositol Metabolism
PathBank:SMP0087258 Phosphatidylinositol Phosphate Metabolism
PathBank:SMP0087410 Phosphatidylinositol Phosphate Metabolism
PathBank:SMP0120865 Joubert Syndrome
PathBank:SMP0108224 Fc Epsilon Receptor I Signaling in Mast Cells
PathBank:SMP0000011 Inositol Metabolism
PathBank:SMP0000462 Inositol Phosphate Metabolism
PathBank:SMP0000463 Phosphatidylinositol Phosphate Metabolism
PathBank:SMP0002394 Inositol Phosphate Metabolism
PathBank:SMP0087349 Inositol Phosphate Metabolism
PathBank:SMP0063651 Phosphatidylinositol Phosphate Metabolism
PathBank:SMP0108186 Fc Epsilon Receptor I Signaling in Mast Cells
PathBank:SMP0120646 Joubert Syndrome
PathBank:SMP0000358 Fc Epsilon Receptor I Signaling in Mast Cells
PathBank:SMP0087256 Inositol Phosphate Metabolism
PathBank:SMP0087396 Inositol Metabolism
PathBank:SMP0087408 Inositol Phosphate Metabolism
PathBank:SMP0000582 Joubert Syndrome
PathBank:SMP0087335 Inositol Metabolism
PathBank:SMP0087351 Phosphatidylinositol Phosphate Metabolism