EC: 2.7.2.4

aspartate kinase (ATP:L-aspartate 4-phosphotransferase)

enzyme lambda metabolic reaction experiment
uniprot:P08660 [aspartate kinase(Enzyme) mutant E119S E434V His6-tag]

L_Malate + ATP -> 4_Phospho_L_malate + ADP		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 60 mM HEPES, 60 mM KCl, 6 mM MgCl2, 0.25 mM NADH, 0.8 mM phosphoenolpyruvic acid, 2 U/ml lactate dehydrogenase. 2 U/ml pyruvate kinase
PH: 7.5
Temperature: 30
uniprot:P08660 [aspartate kinase(Enzyme) mutant V115A E119S His6-tag]

ATP + L_Malate -> ADP + 4_Phospho_L_malate		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 60 mM HEPES, 60 mM KCl, 6 mM MgCl2, 0.25 mM NADH, 0.8 mM phosphoenolpyruvic acid, 2 U/ml lactate dehydrogenase. 2 U/ml pyruvate kinase
PH: 7.5
Temperature: 30
uniprot:P08660 [aspartate kinase(Enzyme) mutant V115A E119C His6-tag]

ATP + L_Malate -> 4_Phospho_L_malate + ADP		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 60 mM HEPES, 60 mM KCl, 6 mM MgCl2, 0.25 mM NADH, 0.8 mM phosphoenolpyruvic acid, 2 U/ml lactate dehydrogenase. 2 U/ml pyruvate kinase
PH: 7.5
Temperature: 30
uniprot:P10869 [aspartate kinase(Enzyme) mutant H497A]

L_Aspartate + ATP -> ADP + 4_Phospho_L_aspartate		 (( (( (E * kcat) ) * substrate) ) / (Km + substrate)) buffer: 100 mM Hepes, 2.5 mM Phosphoenolpyruvate, 1 mM NADH, 10 mM KCl, 40 mM MgSO4
PH: 7.5
Temperature: 30
uniprot:P10869 [aspartate kinase(Enzyme) mutant E279A]

ATP + L_Aspartate -> 4_Phospho_L_aspartate + ADP		 (( (( (E * kcat) ) * substrate) ) / (Km + substrate)) buffer: 100 mM Hepes, 2.5 mM Phosphoenolpyruvate, 1 mM NADH, 10 mM KCl, 40 mM MgSO4
PH: 7.5
Temperature: 30
uniprot:P10869 [aspartate kinase(Enzyme) mutant K18Q]

ATP + L_Aspartate -> ADP + 4_Phospho_L_aspartate		 (( (( (E * kcat) ) * substrate) ) / (Km + substrate)) buffer: 100 mM Hepes, 2.5 mM Phosphoenolpyruvate, 1 mM NADH, 10 mM KCl, 40 mM MgSO4
PH: 7.5
Temperature: 30
uniprot:P10869 [aspartate kinase(Enzyme) mutant K18R]

ATP + L_Aspartate -> ADP + 4_Phospho_L_aspartate		 (( (( (E * kcat) ) * substrate) ) / (Km + substrate)) buffer: 100 mM Hepes, 2.5 mM Phosphoenolpyruvate, 1 mM NADH, 10 mM KCl, 40 mM MgSO4
PH: 7.5
Temperature: 30
uniprot:P10869 [aspartate kinase(Enzyme) mutant T22A]

L_Aspartate + ATP -> 4_Phospho_L_aspartate + ADP		 (( (( (E * kcat) ) * substrate) ) / (Km + substrate)) buffer: 100 mM Hepes, 2.5 mM Phosphoenolpyruvate, 1 mM NADH, 10 mM KCl, 40 mM MgSO4
PH: 7.5
Temperature: 30
uniprot:P10869 [aspartate kinase(Enzyme) mutant K18A]

ATP + L_Aspartate -> ADP + 4_Phospho_L_aspartate		 (( (( (E * kcat) ) * substrate) ) / (Km + substrate)) buffer: 100 mM Hepes, 2.5 mM Phosphoenolpyruvate, 1 mM NADH, 10 mM KCl, 40 mM MgSO4
PH: 7.5
Temperature: 30
uniprot:P10869 [aspartate kinase(Enzyme) mutant T295V]

L_Aspartate + ATP -> 4_Phospho_L_aspartate + ADP		 (( (( (E * kcat) ) * substrate) ) / (Km + substrate)) buffer: 100 mM Hepes, 2.5 mM Phosphoenolpyruvate, 1 mM NADH, 10 mM KCl, 40 mM MgSO4
PH: 7.5
Temperature: 30
uniprot:P10869 [aspartate kinase(Enzyme) mutant S23A]

L_Aspartate + ATP -> 4_Phospho_L_aspartate + ADP		 (( (( (E * kcat) ) * substrate) ) / (Km + substrate)) buffer: 100 mM Hepes, 2.5 mM Phosphoenolpyruvate, 1 mM NADH, 10 mM KCl, 40 mM MgSO4
PH: 7.5
Temperature: 30
uniprot:P10869 [aspartate kinase(Enzyme) wildtype]

ATP + L_Aspartate -> 4_Phospho_L_aspartate + ADP		 (( (( (E * kcat) ) * substrate) ) / (Km + substrate)) buffer: 100 mM Hepes, 2.5 mM Phosphoenolpyruvate, 1 mM NADH, 10 mM KCl, 40 mM MgSO4, 0.33 U pyruvate kinase, 0.66 U lactate dehydrogenase
PH: 7.5
Temperature: 30
uniprot:P10869 [aspartate kinase(Enzyme) mutant E254A]

L_Aspartate + ATP -> ADP + 4_Phospho_L_aspartate		 (( (( (E * kcat) ) * substrate) ) / (Km + substrate)) buffer: 100 mM Hepes, 2.5 mM Phosphoenolpyruvate, 1 mM NADH, 10 mM KCl, 40 mM MgSO4
PH: 7.5
Temperature: 30
uniprot:P10869 [aspartate kinase(Enzyme) mutant R419A]

ATP + L_Aspartate -> 4_Phospho_L_aspartate + ADP		 (( (( (E * kcat) ) * substrate) ) / (Km + substrate)) buffer: 100 mM Hepes, 2.5 mM Phosphoenolpyruvate, 1 mM NADH, 10 mM KCl, 40 mM MgSO4
PH: 7.5
Temperature: 30
uniprot:P10869 [aspartate kinase(Enzyme) mutant H292Q]

L_Aspartate + ATP -> ADP + 4_Phospho_L_aspartate		 (( (( (E * kcat) ) * substrate) ) / (Km + substrate)) buffer: 100 mM Hepes, 2.5 mM Phosphoenolpyruvate, 1 mM NADH, 10 mM KCl, 40 mM MgSO4
PH: 7.5
Temperature: 30
uniprot:P10869 [aspartate kinase(Enzyme) mutant T22A]

L_Aspartate + ATP -> ADP + 4_Phospho_L_aspartate		 (( (( (E * kcat) ) * substrate) ) / (Km + substrate)) buffer: 100 mM Hepes, 2.5 mM Phosphoenolpyruvate, 1 mM NADH, 10 mM KCl, 40 mM MgSO4
PH: 7.5
Temperature: 30
uniprot:P10869 [aspartate kinase(Enzyme) mutant H292A]

L_Aspartate + ATP -> 4_Phospho_L_aspartate + ADP		 (( (( (E * kcat) ) * substrate) ) / (Km + substrate)) buffer: 100 mM Hepes, 2.5 mM Phosphoenolpyruvate, 1 mM NADH, 10 mM KCl, 40 mM MgSO4
PH: 7.5
Temperature: 30
uniprot:P10869 [aspartate kinase(Enzyme) mutant K18R]

L_Aspartate + ATP -> 4_Phospho_L_aspartate + ADP		 (( (( (E * kcat) ) * substrate) ) / (Km + substrate)) buffer: 100 mM Hepes, 2.5 mM Phosphoenolpyruvate, 1 mM NADH, 10 mM KCl, 40 mM MgSO4
PH: 7.5
Temperature: 30
uniprot:P10869 [aspartate kinase(Enzyme) mutant K18A]

ATP + L_Aspartate -> ADP + 4_Phospho_L_aspartate		 (( (( (E * kcat) ) * substrate) ) / (Km + substrate)) buffer: 100 mM Hepes, 2.5 mM Phosphoenolpyruvate, 1 mM NADH, 10 mM KCl, 40 mM MgSO4
PH: 7.5
Temperature: 30
uniprot:P10869 [aspartate kinase(Enzyme) mutant K18Q]

ATP + L_Aspartate -> 4_Phospho_L_aspartate + ADP		 (( (( (E * kcat) ) * substrate) ) / (Km + substrate)) buffer: 100 mM Hepes, 2.5 mM Phosphoenolpyruvate, 1 mM NADH, 10 mM KCl, 40 mM MgSO4
PH: 7.5
Temperature: 30
uniprot:P10869 [aspartate kinase(Enzyme) wildtype]

ATP + L_Aspartate -> ADP + 4_Phospho_L_aspartate		 (( (( (E * kcat) ) * substrate) ) / (Km + substrate)) buffer: 100 mM Hepes, 2.5 mM Phosphoenolpyruvate, 1 mM NADH, 10 mM KCl, 40 mM MgSO4
PH: 7.5
Temperature: 30
uniprot:P10869 [aspartate kinase(Enzyme) mutant S23A]

ATP + L_Aspartate -> ADP + 4_Phospho_L_aspartate		 (( (( (E * kcat) ) * substrate) ) / (Km + substrate)) buffer: 100 mM Hepes, 2.5 mM Phosphoenolpyruvate, 1 mM NADH, 10 mM KCl, 40 mM MgSO4
PH: 7.5
Temperature: 30
uniprot:P10869 [aspartate kinase(Enzyme) mutant T295V]

ATP + L_Aspartate -> ADP + 4_Phospho_L_aspartate		 (( (( (E * kcat) ) * substrate) ) / (Km + substrate)) buffer: 100 mM Hepes, 2.5 mM Phosphoenolpyruvate, 1 mM NADH, 10 mM KCl, 40 mM MgSO4
PH: 7.5
Temperature: 30
uniprot:P10869 [aspartate kinase(Enzyme) mutant H497A]

L_Aspartate + ATP -> ADP + 4_Phospho_L_aspartate		 (( (( (E * kcat) ) * substrate) ) / (Km + substrate)) buffer: 100 mM Hepes, 2.5 mM Phosphoenolpyruvate, 1 mM NADH, 10 mM KCl, 40 mM MgSO4
PH: 7.5
Temperature: 30
uniprot:P10869 [aspartate kinase(Enzyme) mutant E279A]

ATP + L_Aspartate -> 4_Phospho_L_aspartate + ADP		 (( (( (E * kcat) ) * substrate) ) / (Km + substrate)) buffer: 100 mM Hepes, 2.5 mM Phosphoenolpyruvate, 1 mM NADH, 10 mM KCl, 40 mM MgSO4
PH: 7.5
Temperature: 30
uniprot:P10869 [aspartate kinase(Enzyme) mutant R419A]

ATP + L_Aspartate -> 4_Phospho_L_aspartate + ADP		 (( (( (E * kcat) ) * substrate) ) / (Km + substrate)) buffer: 100 mM Hepes, 2.5 mM Phosphoenolpyruvate, 1 mM NADH, 10 mM KCl, 40 mM MgSO4
PH: 7.5
Temperature: 30
uniprot:P10869 [aspartate kinase(Enzyme) mutant H292A]

ATP + L_Aspartate -> ADP + 4_Phospho_L_aspartate		 (( (( (E * kcat) ) * substrate) ) / (Km + substrate)) buffer: 100 mM Hepes, 2.5 mM Phosphoenolpyruvate, 1 mM NADH, 10 mM KCl, 40 mM MgSO4
PH: 7.5
Temperature: 30
uniprot:P10869 [aspartate kinase(Enzyme) mutant H292Q]

L_Aspartate + ATP -> ADP + 4_Phospho_L_aspartate		 (( (( (E * kcat) ) * substrate) ) / (Km + substrate)) buffer: 100 mM Hepes, 2.5 mM Phosphoenolpyruvate, 1 mM NADH, 10 mM KCl, 40 mM MgSO4
PH: 7.5
Temperature: 30
uniprot:P10869 [aspartate kinase(Enzyme) mutant E254A]

ATP + L_Aspartate -> 4_Phospho_L_aspartate + ADP		 (( (( (E * kcat) ) * substrate) ) / (Km + substrate)) buffer: 100 mM Hepes, 2.5 mM Phosphoenolpyruvate, 1 mM NADH, 10 mM KCl, 40 mM MgSO4
PH: 7.5
Temperature: 30
uniprot:P10869 [L-Threonine]

L_Aspartate + ATP -> 4_Phospho_L_aspartate + ADP		 (( (( (E * kcat) ) * substrate) ) / (( (Km * (1 + (Inhibitor / Kis))) ) + (substrate * (1 + (Inhibitor / Kic))))) buffer: 100 mM Hepes, 2.5 mM Phosphoenolpyruvate, 1 mM NADH, 10 mM KCl, 40 mM MgSO4
PH: 7.5
Temperature: 30
uniprot:P10869 [L-Threonine]

L_Aspartate + ATP -> ADP + 4_Phospho_L_aspartate		 (( (( (E * kcat) ) * substrate) ) / (( (Km * (1 + (Inhibitor / Kis))) ) + (substrate * (1 + (Inhibitor / Kic))))) buffer: 100 mM Hepes, 2.5 mM Phosphoenolpyruvate, 1 mM NADH, 10 mM KCl, 40 mM MgSO4
PH: 7.5
Temperature: 30
uniprot:P10869 [L-Threonine]

ATP + L_Aspartate -> 4_Phospho_L_aspartate + ADP		 (( (( (E * kcat) ) * substrate) ) / (( (Km * (1 + (Inhibitor / Kis))) ) + (substrate * (1 + (Inhibitor / Kic))))) buffer: 100 mM Hepes, 2.5 mM Phosphoenolpyruvate, 1 mM NADH, 10 mM KCl, 40 mM MgSO4
PH: 7.5
Temperature: 30
uniprot:P10869 [L-Threonine]

L_Aspartate + ATP -> 4_Phospho_L_aspartate + ADP		 (( (( (E * kcat) ) * substrate) ) / (Km + (substrate * (1 + (Inhibitor / Ki))))) buffer: 100 mM Hepes, 2.5 mM Phosphoenolpyruvate, 1 mM NADH, 10 mM KCl, 40 mM MgSO4
PH: 7.5
Temperature: 30
uniprot:P08660 [aspartate kinase(Enzyme) wildtype isoenzyme AKIII]

L_Aspartate + ATP -> 4_Phospho_L_aspartate + ADP		 (( (Vmax * A) ) / (( (A + Km) ) + (( (A ^ 2) ) / Ki))) buffer: 20 mM potassium phosphate, 10 mM beta-mercaptoethanol, 0.6 mM NADH, 1.5 mM phosphoenolpyruvate, 0.15 M KCl, 0.02 mg/ml pyruvate kinase, 0.01 mg/ml lactate dehydrogenase, 10 mM magnesium acetate
PH: 7.2
Temperature: 25
uniprot:P08660 [aspartate kinase(Enzyme) wildtype isoenzyme AKIII]

L_Aspartate + ATP -> ADP + 4_Phospho_L_aspartate		 (( (Vmax * A) ) / (Km + A)) buffer: 20 mM potassium phosphate, 10 mM beta-mercaptoethanol, 0.6 mM NADH, 1.5 mM phosphoenolpyruvate, 0.15 M KCl, 0.02 mg/ml pyruvate kinase, 0.01 mg/ml lactate dehydrogenase, 30 mM magnesium acetate
PH: 7.2
Temperature: 25
uniprot:P08660 [aspartate kinase(Enzyme) mutant isoenzyme AKIII K8R]

ATP + L_Aspartate -> 4_Phospho_L_aspartate + ADP		 (( (Vmax * A) ) / (Km + A)) buffer: 20 mM potassium phosphate, 10 mM beta-mercaptoethanol, 0.6 mM NADH, 1.5 mM phosphoenolpyruvate, 0.15 M KCl, 0.02 mg/ml pyruvate kinase, 0.01 mg/ml lactate dehydrogenase, 30 mM magnesium acetate
PH: 7.2
Temperature: 25
uniprot:P08660 [aspartate kinase(Enzyme) mutant isoenzyme AKIII D202E]

L_Aspartate + ATP -> 4_Phospho_L_aspartate + ADP		 (( (Vmax * A) ) / (Km + A)) buffer: 20 mM potassium phosphate, 10 mM beta-mercaptoethanol, 0.6 mM NADH, 1.5 mM phosphoenolpyruvate, 0.15 M KCl, 0.02 mg/ml pyruvate kinase, 0.01 mg/ml lactate dehydrogenase, 30 mM magnesium acetate
PH: 7.2
Temperature: 25
uniprot:P08660 [aspartate kinase(Enzyme) mutant isoenzyme AKIII R198K]

L_Aspartate + ATP -> 4_Phospho_L_aspartate + ADP		 (( (Vmax * A) ) / (Km + A)) buffer: 20 mM potassium phosphate, 10 mM beta-mercaptoethanol, 0.6 mM NADH, 1.5 mM phosphoenolpyruvate, 0.15 M KCl, 0.02 mg/ml pyruvate kinase, 0.01 mg/ml lactate dehydrogenase, 30 mM magnesium acetate
PH: 7.2
Temperature: 25
uniprot:P08660 [aspartate kinase(Enzyme) mutant isoenzyme AKIII E119D]

ATP + L_Aspartate -> ADP + 4_Phospho_L_aspartate		 (( (Vmax * A) ) / (Km + A)) buffer: 20 mM potassium phosphate, 10 mM beta-mercaptoethanol, 0.6 mM NADH, 1.5 mM phosphoenolpyruvate, 0.15 M KCl, 0.02 mg/ml pyruvate kinase, 0.01 mg/ml lactate dehydrogenase, 30 mM magnesium acetate
PH: 7.2
Temperature: 25
uniprot:P08660 [Lysine]

L_Aspartate + ATP -> 4_Phospho_L_aspartate + ADP		 (Vmax * (1 - (( (I ^ N) ) / (( (IC50 ^ N) ) + (I ^ N))))) buffer: 20 mM potassium phosphate, 10 mM beta-mercaptoethanol, 0.6 mM NADH, 1.5 mM phosphoenolpyruvate, 0.15 M KCl, 0.02 mg/ml pyruvate kinase, 0.01 mg/ml lactate dehydrogenase, 30 mM magnesium acetate
PH: 7.2
Temperature: 25
uniprot:P08660 [Lysine]

ATP + L_Aspartate -> ADP + 4_Phospho_L_aspartate		 (Vmax * (1 - (( (I ^ N) ) / (( (IC50 ^ N) ) + (I ^ N))))) buffer: 20 mM potassium phosphate, 10 mM beta-mercaptoethanol, 0.6 mM NADH, 1.5 mM phosphoenolpyruvate, 0.15 M KCl, 0.02 mg/ml pyruvate kinase, 0.01 mg/ml lactate dehydrogenase, 30 mM magnesium acetate
PH: 7.2
Temperature: 25
uniprot:P08660 [Lysine]

L_Aspartate + ATP -> ADP + 4_Phospho_L_aspartate		 (Vmax * (1 - (( (I ^ N) ) / (( (IC50 ^ N) ) + (I ^ N))))) buffer: 20 mM potassium phosphate, 10 mM beta-mercaptoethanol, 0.6 mM NADH, 1.5 mM phosphoenolpyruvate, 0.15 M KCl, 0.02 mg/ml pyruvate kinase, 0.01 mg/ml lactate dehydrogenase, 30 mM magnesium acetate
PH: 7.2
Temperature: 25
uniprot:P08660 [aspartate kinase(Enzyme) mutant isoenzyme AKIII E119D]

L_Aspartate + ATP -> 4_Phospho_L_aspartate + ADP		 (( (Vmax * A) ) / (( (A + Km) ) + (( (A ^ 2) ) / Ki))) buffer: 20 mM potassium phosphate, 10 mM beta-mercaptoethanol, 0.6 mM NADH, 1.5 mM phosphoenolpyruvate, 0.15 M KCl, 0.02 mg/ml pyruvate kinase, 0.01 mg/ml lactate dehydrogenase, 10 mM magnesium acetate
PH: 7.2
Temperature: 25
uniprot:P08660 [aspartate kinase(Enzyme) mutant isoenzyme AKIII R198K]

L_Aspartate + ATP -> ADP + 4_Phospho_L_aspartate		 (( (Vmax * A) ) / (( (A + Km) ) + (( (A ^ 2) ) / Ki))) buffer: 20 mM potassium phosphate, 10 mM beta-mercaptoethanol, 0.6 mM NADH, 1.5 mM phosphoenolpyruvate, 0.15 M KCl, 0.02 mg/ml pyruvate kinase, 0.01 mg/ml lactate dehydrogenase, 10 mM magnesium acetate
PH: 7.2
Temperature: 25
uniprot:P08660 [aspartate kinase(Enzyme) mutant isoenzyme AKIII K8R]

L_Aspartate + ATP -> ADP + 4_Phospho_L_aspartate		 (( (Vmax * A) ) / (Km + A)) buffer: 20 mM potassium phosphate, 10 mM beta-mercaptoethanol, 0.6 mM NADH, 1.5 mM phosphoenolpyruvate, 0.15 M KCl, 0.02 mg/ml pyruvate kinase, 0.01 mg/ml lactate dehydrogenase, 10 mM magnesium acetate
PH: 7.2
Temperature: 25
uniprot:P08660 [aspartate kinase(Enzyme) mutant isoenzyme AKIII D202E]

ATP + L_Aspartate -> 4_Phospho_L_aspartate + ADP		 (( (Vmax * A) ) / (Km + A)) buffer: 20 mM potassium phosphate, 10 mM beta-mercaptoethanol, 0.6 mM NADH, 1.5 mM phosphoenolpyruvate, 0.15 M KCl, 0.02 mg/ml pyruvate kinase, 0.01 mg/ml lactate dehydrogenase, 10 mM magnesium acetate
PH: 7.2
Temperature: 25
uniprot:O81852 [L-Threonine]

ATP + L_Aspartate -> 4_Phospho_L_aspartate + ADP		 (( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) buffer: 100 mM Tris-HCl, 400 mM Hydroxylamine-KOH, 20 mM MgCl2, 150 mM KCl
PH: 8
Temperature: 37
uniprot:O81852 [L-Threonine]

ATP + L_Aspartate -> 4_Phospho_L_aspartate + ADP		 (( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) buffer: 100 mM Tris-HCl, 400 mM Hydroxylamine-KOH, 20 mM MgCl2, 150 mM KCl
PH: 8
Temperature: 37
uniprot:Q9SA18 [Alanine]

ATP + L_Aspartate -> 4_Phospho_L_aspartate + ADP		 (( (( (kcat * E) ) * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) buffer: 50 mM Hepes, 150 mM KCl, 20 mM MgCl2, 200 µM NADPH, 0.8 µM Aspartate semialdehyde dehydrogenase
PH: 8
Temperature: 30
uniprot:Q9SA18 [aspartate kinase(Enzyme) wildtype AK I]

L_Aspartate + ATP -> 4_Phospho_L_aspartate + ADP		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM Hepes, 150 mM KCl, 20 mM MgCl2, 200 µM NADPH, 0.8 µM Aspartate semialdehyde dehydrogenase
PH: 8
Temperature: 30
uniprot:Q9SA18 [aspartate kinase(Enzyme) wildtype AK I]

L_Aspartate + ATP -> 4_Phospho_L_aspartate + ADP		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM Hepes, 150 mM KCl, 20 mM MgCl2, 200 µM NADPH, 0.8 µM Aspartate semialdehyde dehydrogenase
PH: 8
Temperature: 30
uniprot:Q9SA18 [Leucine]

ATP + L_Aspartate -> 4_Phospho_L_aspartate + ADP		 (( (( (kcat * E) ) * (I ^ n)) ) / (( (I_half ^ n) ) + (I ^ n))) buffer: 50 mM Hepes, 150 mM KCl, 20 mM MgCl2, 200 µM NADPH, 0.8 µM Aspartate semialdehyde dehydrogenase
PH: 8
Temperature: 30
uniprot:Q9SA18 [Threonine]

ATP + L_Aspartate -> ADP + 4_Phospho_L_aspartate		 (( (( (kcat * E) ) * (I ^ n)) ) / (( (I_half ^ n) ) + (I ^ n))) buffer: 50 mM Hepes, 150 mM KCl, 20 mM MgCl2, 200 µM NADPH, 0.8 µM Aspartate semialdehyde dehydrogenase
PH: 8
Temperature: 30
uniprot:O81852 [Alanine]

L_Aspartate + ATP -> 4_Phospho_L_aspartate + ADP		 (( (( (kcat * E) ) * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) buffer: 50 mM Hepes, 150 mM KCl, 20 mM MgCl2, 200 µM NADPH, 0.8 µM Aspartate semialdehyde dehydrogenase
PH: 8
Temperature: 30
uniprot:Q9SA18 [Alanine]

ATP + L_Aspartate -> ADP + 4_Phospho_L_aspartate		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 50 mM Hepes, 150 mM KCl, 20 mM MgCl2, 200 µM NADPH, 0.8 µM Aspartate semialdehyde dehydrogenase
PH: 8
Temperature: 30
uniprot:O81852 [Alanine]

ATP + L_Aspartate -> 4_Phospho_L_aspartate + ADP		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM Hepes, 150 mM KCl, 20 mM MgCl2, 200 µM NADPH, 0.8 µM Aspartate semialdehyde dehydrogenase
PH: 8
Temperature: 30
uniprot:Q9SA18 [Alanine]

ATP + L_Aspartate -> 4_Phospho_L_aspartate + ADP		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM Hepes, 150 mM KCl, 20 mM MgCl2, 200 µM NADPH, 0.8 µM Aspartate semialdehyde dehydrogenase
PH: 8
Temperature: 30
uniprot:Q9SA18 [Cysteine]

ATP + L_Aspartate -> ADP + 4_Phospho_L_aspartate		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 50 mM Hepes, 150 mM KCl, 20 mM MgCl2, 200 µM NADPH, 0.8 µM Aspartate semialdehyde dehydrogenase
PH: 8
Temperature: 30
uniprot:O81852 [aspartate kinase(Enzyme) wildtype AK II]

L_Aspartate + ATP -> ADP + 4_Phospho_L_aspartate		 (( (( (kcat * E) ) * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) buffer: 50 mM Hepes, 150 mM KCl, 20 mM MgCl2, 200 µM NADPH, 0.8 µM Aspartate semialdehyde dehydrogenase
PH: 8
Temperature: 30
uniprot:O81852 [aspartate kinase(Enzyme) wildtype AK II]

ATP + L_Aspartate -> ADP + 4_Phospho_L_aspartate		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM Hepes, 150 mM KCl, 20 mM MgCl2, 200 µM NADPH, 0.8 µM Aspartate semialdehyde dehydrogenase
PH: 8
Temperature: 30
uniprot:O81852 [Serine]

ATP + L_Aspartate -> ADP + 4_Phospho_L_aspartate		 (( (( (kcat * E) ) * (A ^ n)) ) / (( (A_half ^ n) ) + (A ^ n))) buffer: 50 mM Hepes, 150 mM KCl, 20 mM MgCl2, 200 µM NADPH, 0.8 µM Aspartate semialdehyde dehydrogenase
PH: 8
Temperature: 30
uniprot:O81852 [Alanine]

L_Aspartate + ATP -> ADP + 4_Phospho_L_aspartate		 (( (( (kcat * E) ) * (A ^ n)) ) / (( (A_half ^ n) ) + (A ^ n))) buffer: 50 mM Hepes, 150 mM KCl, 20 mM MgCl2, 200 µM NADPH, 0.8 µM Aspartate semialdehyde dehydrogenase
PH: 8
Temperature: 30
uniprot:O81852 [Cysteine]

L_Aspartate + ATP -> ADP + 4_Phospho_L_aspartate		 (( (( (kcat * E) ) * (A ^ n)) ) / (( (A_half ^ n) ) + (A ^ n))) buffer: 50 mM Hepes, 150 mM KCl, 20 mM MgCl2, 200 µM NADPH, 0.8 µM Aspartate semialdehyde dehydrogenase
PH: 8
Temperature: 30
uniprot:O81852 [Threonine]

L_Aspartate + ATP -> 4_Phospho_L_aspartate + ADP		 (( (( (kcat * E) ) * (I ^ n)) ) / (( (I_half ^ n) ) + (I ^ n))) buffer: 50 mM Hepes, 150 mM KCl, 20 mM MgCl2, 200 µM NADPH, 0.8 µM Aspartate semialdehyde dehydrogenase
PH: 8
Temperature: 30
uniprot:O81852 [aspartate kinase(Enzyme) wildtype AK II]

L_Aspartate + ATP -> ADP + 4_Phospho_L_aspartate		 (( (( (kcat * E) ) * (A ^ n)) ) / (( (A_half ^ n) ) + (A ^ n))) buffer: 50 mM Hepes, 150 mM KCl, 20 mM MgCl2, 200 µM NADPH, 0.8 µM Aspartate semialdehyde dehydrogenase
PH: 8
Temperature: 30
uniprot:O81852 [Isoleucine]

ATP + L_Aspartate -> 4_Phospho_L_aspartate + ADP		 (( (( (kcat * E) ) * (A ^ n)) ) / (( (A_half ^ n) ) + (A ^ n))) buffer: 50 mM Hepes, 150 mM KCl, 20 mM MgCl2, 200 µM NADPH, 0.8 µM Aspartate semialdehyde dehydrogenase
PH: 8
Temperature: 30
uniprot:Q9SA18 [Serine]

ATP + L_Aspartate -> 4_Phospho_L_aspartate + ADP		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 50 mM Hepes, 150 mM KCl, 20 mM MgCl2, 200 µM NADPH, 0.8 µM Aspartate semialdehyde dehydrogenase
PH: 8
Temperature: 30
uniprot:Q9SA18 [aspartate kinase(Enzyme) wildtype AK I]

L_Aspartate + ATP -> ADP + 4_Phospho_L_aspartate		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 50 mM Hepes, 150 mM KCl, 20 mM MgCl2, 200 µM NADPH, 0.8 µM Aspartate semialdehyde dehydrogenase
PH: 8
Temperature: 30
uniprot:Q9SA18 [Isoleucine]

L_Aspartate + ATP -> ADP + 4_Phospho_L_aspartate		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 50 mM Hepes, 150 mM KCl, 20 mM MgCl2, 200 µM NADPH, 0.8 µM Aspartate semialdehyde dehydrogenase
PH: 8
Temperature: 30
uniprot:Q9SA18 [Alanine]

L_Aspartate + ATP -> ADP + 4_Phospho_L_aspartate		 (( (( (kcat * E) ) * (I ^ n)) ) / (( (I_half ^ n) ) + (I ^ n))) buffer: 50 mM Hepes, 150 mM KCl, 20 mM MgCl2, 200 µM NADPH, 0.8 µM Aspartate semialdehyde dehydrogenase
PH: 8
Temperature: 30
uniprot:Q9SA18 [Alanine]

L_Aspartate + ATP -> ADP + 4_Phospho_L_aspartate		 (( (( (kcat * E) ) * (A ^ n)) ) / (( (A_half ^ n) ) + (A ^ n))) buffer: 50 mM Hepes, 150 mM KCl, 20 mM MgCl2, 200 µM NADPH, 0.8 µM Aspartate semialdehyde dehydrogenase
PH: 8
Temperature: 30
uniprot:O81852 [Leucine]

L_Aspartate + ATP -> 4_Phospho_L_aspartate + ADP		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 50 mM Hepes, 150 mM KCl, 20 mM MgCl2, 200 µM NADPH, 0.8 µM Aspartate semialdehyde dehydrogenase
PH: 8
Temperature: 30
uniprot:O81852 [Alanine]

ATP + L_Aspartate -> 4_Phospho_L_aspartate + ADP		 (( (( (kcat * E) ) * (A ^ n)) ) / (( (A_half ^ n) ) + (A ^ n))) buffer: 50 mM Hepes, 150 mM KCl, 20 mM MgCl2, 200 µM NADPH, 0.8 µM Aspartate semialdehyde dehydrogenase
PH: 8
Temperature: 30
uniprot:O81852 [Alanine]

ATP + L_Aspartate -> ADP + 4_Phospho_L_aspartate		 (( (( (kcat * E) ) * (I ^ n)) ) / (( (I_half ^ n) ) + (I ^ n))) buffer: 50 mM Hepes, 150 mM KCl, 20 mM MgCl2, 200 µM NADPH, 0.8 µM Aspartate semialdehyde dehydrogenase
PH: 8
Temperature: 30
uniprot:Q9LYU8 [Lysine]

L_Aspartate + ATP -> ADP + 4_Phospho_L_aspartate		 (( (( (kcat * E) ) * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) buffer: 50 mM Hepes, 150 mM KCl, 20 mM MgCl2, 200 µM NADPH, 1 µM aspartate semialdehyde dehydrogenase
PH: 8
Temperature: 30
uniprot:Q9LYU8 [Lysine]

ATP + L_Aspartate -> ADP + 4_Phospho_L_aspartate		 (( (( (kcat * E) ) * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) buffer: 50 mM Hepes, 150 mM KCl, 20 mM MgCl2, 200 µM NADPH, 1 µM aspartate semialdehyde dehydrogenase
PH: 8
Temperature: 30
uniprot:Q9LYU8 [Lysine]

ATP + L_Aspartate -> ADP + 4_Phospho_L_aspartate		 (( (( (kcat * E) ) * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) buffer: 50 mM Hepes, 150 mM KCl, 20 mM MgCl2, 200 µM NADPH, 1 µM aspartate semialdehyde dehydrogenase
PH: 8
Temperature: 30
uniprot:Q9LYU8 [Lysine]

ATP + L_Aspartate -> ADP + 4_Phospho_L_aspartate		 (( (( (kcat * E) ) * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) buffer: 50 mM Hepes, 150 mM KCl, 20 mM MgCl2, 200 µM NADPH, 1 µM aspartate semialdehyde dehydrogenase
PH: 8
Temperature: 30
uniprot:O23653 [Lysine]

L_Aspartate + ATP -> 4_Phospho_L_aspartate + ADP		 (( (( (kcat * E) ) * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) buffer: 50 mM Hepes, 150 mM KCl, 20 mM MgCl2, 200 µM NADPH, 1 µM aspartate semialdehyde dehydrogenase
PH: 8
Temperature: 30
uniprot:Q9S702 [Lysine]

ATP + L_Aspartate -> ADP + 4_Phospho_L_aspartate		 (( (( (kcat * E) ) * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) buffer: 50 mM Hepes, 150 mM KCl, 20 mM MgCl2, 200 µM NADPH, 1 µM aspartate semialdehyde dehydrogenase
PH: 8
Temperature: 30
uniprot:Q9LYU8 [Lysine]

L_Aspartate + ATP -> ADP + 4_Phospho_L_aspartate		 (( (( (kcat * E) ) * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) buffer: 50 mM Hepes, 150 mM KCl, 20 mM MgCl2, 200 µM NADPH, 1 µM aspartate semialdehyde dehydrogenase
PH: 8
Temperature: 30
uniprot:O81852 [Threonine]

L_Aspartate + ATP -> ADP + 4_Phospho_L_aspartate		 (( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) buffer: 100 mM Tris-HCl, 400 mM Hydroxylamine-KOH, 20 mM MgCl2, 150 mM KCl
PH: 8
Temperature: 37
uniprot:O81852 [Threonine]

L_Aspartate + ATP -> 4_Phospho_L_aspartate + ADP		 (( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) buffer: 100 mM Tris-HCl, 400 mM Hydroxylamine-KOH, 20 mM MgCl2, 150 mM KCl
PH: 8
Temperature: 37
- [aspartate kinase(Enzyme) wildtype]

ATP + L_Aspartate -> 4_Phospho_L_aspartate + ADP		 (( (Vmax * B) ) / (Km + B)) buffer: 93.1 mM tris(hydroxymethyl)aminoethane (Tris)-hydrochloride, 3.1 mM MgSO4, 0.8 M NH2OH-HCl
PH: 8.1
Temperature: 29
- [aspartate kinase(Enzyme) wildtype]

L_Aspartate + ATP -> ADP + 4_Phospho_L_aspartate		 (( (Vmax * A) ) / (Km + A)) buffer: 93.1 mM tris(hydroxymethyl)aminoethane (Tris)-hydrochloride, 3.1 mM MgSO4, 0.8 M NH2OH-HCl
PH: 8.1
Temperature: 29

Pathways

pathway id name
BioCyc:META_PWY-7977 L-methionine biosynthesis IV (archaea)
BioCyc:META_PWY-5345 superpathway of L-methionine biosynthesis (by sulfhydrylation)
BioCyc:META_PWY-6165 chorismate biosynthesis II (archaea)
BioCyc:META_PWY0-781 aspartate superpathway
BioCyc:META_PWY-6160 3-dehydroquinate biosynthesis II (archaea)
BioCyc:ARA_THRESYN-PWY superpathway of L-threonine biosynthesis
BioCyc:ARA_HOMOSERSYN-PWY L-homoserine biosynthesis
BioCyc:MTBH37RV_PWY-5345 superpathway of L-methionine biosynthesis (by sulfhydrylation)
BioCyc:MTBH37RV_PWY-3001 superpathway of L-isoleucine biosynthesis I
BioCyc:HPY_DAPLYSINESYN-PWY L-lysine biosynthesis I
BioCyc:BSUB_METSYN-PWY superpathway of L-homoserine and L-methionine biosynthesis
BioCyc:ECOL316407_P4-PWY superpathway of lysine, threonine and methionine biosynthesis I
BioCyc:ECOL316407_METSYN-PWY homoserine and methionine biosynthesis
BioCyc:ECOL316407_PWY0-781 aspartate superpathway
BioCyc:CORYNE_PWY0-781 aspartate superpathway
BioCyc:CORYNE_MET-SAM-PWY superpathway of S-adenosyl-L-methionine biosynthesis
BioCyc:META_HOMOSERSYN-PWY L-homoserine biosynthesis
BioCyc:META_DAPLYSINESYN-PWY L-lysine biosynthesis I
BioCyc:META_PWY-724 superpathway of L-lysine, L-threonine and L-methionine biosynthesis II
BioCyc:ECO_P4-PWY superpathway of L-lysine, L-threonine and L-methionine biosynthesis I
BioCyc:ECO_DAPLYSINESYN-PWY L-lysine biosynthesis I
BioCyc:ARA_PWY-5097 L-lysine biosynthesis VI
BioCyc:MTBH37RV_HOMOSERSYN-PWY L-homoserine biosynthesis
BioCyc:MTBH37RV_THRESYN-PWY superpathway of L-threonine biosynthesis
BioCyc:ECOL316407_DAPLYSINESYN-PWY lysine biosynthesis I
BioCyc:ECOL316407_MET-SAM-PWY superpathway of S-adenosyl-L-methionine biosynthesis
BioCyc:CORYNE_METSYN-PWY L-homoserine and L-methionine biosynthesis
BioCyc:CORYNE_PWY-5345 superpathway of L-methionine biosynthesis (by sulfhydrylation)
BioCyc:CORYNE_THRESYN-PWY superpathway of L-threonine biosynthesis
BioCyc:CORYNE_PWY-2942 L-lysine biosynthesis III
BioCyc:CORYNE_DAPLYSINESYN-PWY L-lysine biosynthesis I
BioCyc:META_PWY-5097 L-lysine biosynthesis VI
BioCyc:META_PWY-2941 L-lysine biosynthesis II
BioCyc:META_PWY-5347 superpathway of L-methionine biosynthesis (transsulfuration)
BioCyc:ECO_MET-SAM-PWY superpathway of S-adenosyl-L-methionine biosynthesis
BioCyc:ECO_THRESYN-PWY superpathway of L-threonine biosynthesis
BioCyc:YEAST_HOMOSERSYN-PWY homoserine biosynthesis
BioCyc:ARA_PWY-724 superpathway of L-lysine, L-threonine and L-methionine biosynthesis II
BioCyc:MTBCDC1551_DAPLYSINESYN-PWY L-lysine biosynthesis I
BioCyc:MTBCDC1551_MET-SAM-PWY superpathway of S-adenosyl-L-methionine biosynthesis
BioCyc:BSUB_PWY-2941 L-lysine biosynthesis II
BioCyc:ECOL316407_HOMOSERSYN-PWY homoserine biosynthesis
BioCyc:ECOL316407_THRESYN-PWY threonine biosynthesis
BioCyc:CORYNE_PWY-5347 superpathway of L-methionine biosynthesis (transsulfuration)
BioCyc:CORYNE_HOMOSERSYN-PWY L-homoserine biosynthesis
BioCyc:META_METSYN-PWY superpathway of L-homoserine and L-methionine biosynthesis
BioCyc:META_P4-PWY superpathway of L-lysine, L-threonine and L-methionine biosynthesis I
BioCyc:META_THRESYN-PWY superpathway of L-threonine biosynthesis
BioCyc:META_MET-SAM-PWY superpathway of S-adenosyl-L-methionine biosynthesis
BioCyc:META_PWY-3001 superpathway of L-isoleucine biosynthesis I
BioCyc:ECO_HOMOSERSYN-PWY L-homoserine biosynthesis
BioCyc:ECO_PWY0-781 aspartate superpathway
BioCyc:ECO_METSYN-PWY superpathway of L-homoserine and L-methionine biosynthesis
BioCyc:ARA_PWY-3001 superpathway of L-isoleucine biosynthesis I
BioCyc:MTBH37RV_DAPLYSINESYN-PWY L-lysine biosynthesis I
BioCyc:MTBCDC1551_METSYN-PWY L-homoserine and L-methionine biosynthesis
BioCyc:MTBCDC1551_HOMOSERSYN-PWY L-homoserine biosynthesis
BioCyc:HPY_HOMOSERSYN-PWY L-homoserine biosynthesis
BioCyc:BSUB_MET-SAM-PWY superpathway of S-adenosyl-L-methionine biosynthesis
BioCyc:BSUB_HOMOSERSYN-PWY L-homoserine biosynthesis
BioCyc:CORYNE_PWY-3001 superpathway of L-isoleucine biosynthesis I
BioCyc:CORYNE_P4-PWY superpathway of L-lysine, L-threonine and L-methionine biosynthesis I
PathBank:SMP0000794 Lysine Biosynthesis
PathBank:SMP0000834 Methionine Biosynthesis
PathBank:SMP0000837 Threonine Biosynthesis
PathBank:SMP0000992 Secondary Metabolites: Threonine Biosynthesis from Aspartate
PlantCyc:ARA_HOMOSERSYN-PWY L-homoserine biosynthesis
PlantCyc:PLANT_PWY-724 superpathway of L-lysine, L-threonine and L-methionine biosynthesis II
PlantCyc:ARA_PWY-3001 superpathway of L-isoleucine biosynthesis I
PlantCyc:ARA_PWY-5097 L-lysine biosynthesis VI
PlantCyc:ARA_PWY-724 superpathway of L-lysine, L-threonine and L-methionine biosynthesis II
PlantCyc:PLANT_PWY-5097 L-lysine biosynthesis VI
PlantCyc:PLANT_PWY-3001 superpathway of L-isoleucine biosynthesis I
PlantCyc:PLANT_THRESYN-PWY superpathway of L-threonine biosynthesis
PlantCyc:ARA_THRESYN-PWY superpathway of L-threonine biosynthesis
PlantCyc:PLANT_HOMOSERSYN-PWY L-homoserine biosynthesis
PathBank:SMP0063480 Monobactam Biosynthesis
PathBank:SMP0002309 Cysteine Metabolism
PathBank:SMP0002434 Lysine Metabolism
PathBank:SMP0002439 Methionine Metabolism
PathBank:SMP0002310 Methionine Metabolism and Salvage
PathBank:SMP0002322 Threonine Metabolism
PathBank:SMP0002444 Threonine Metabolism