EC: 2.7.1.6

galactokinase (ATP:D-galactose 1-phosphotransferase)

enzyme lambda metabolic reaction experiment
- [galactokinase(Enzyme) wildtype]

ATP + D_Galactose -> ADP + alpha_D_Galactose_1_phosphate		 (( (Vmax * S) ) / (S + Km)) buffer: 200 mM Tris-hydrochloride, 5 mM NaF, 5 mM MgCl2, 10 mM Mercaptoethanol
PH: 7.1
Temperature: 37
- [galactokinase(Enzyme) wildtype]

D_Galactose + ATP -> ADP + alpha_D_Galactose_1_phosphate		 (( (Vmax * S) ) / (S + Km)) buffer: 200 mM Tris-hydrochloride, 5 mM NaF, 5 mM MgCl2, 10 mM Mercaptoethanol
PH: 7.1
Temperature: 37
- [galactokinase(Enzyme) wildtype]

ATP + D_Galactose -> ADP + alpha_D_Galactose_1_phosphate		 (( (Vmax * S) ) / (S + Km)) buffer: 200 mM Tris-hydrochloride, 5 mM NaF, 5 mM MgCl2, 10 mM Mercaptoethanol
PH: 7.1
Temperature: 37
- [galactokinase(Enzyme) wildtype]

D_Galactose + ATP -> ADP + alpha_D_Galactose_1_phosphate		 (( (Vmax * S) ) / (S + Km)) buffer: 200 mM Tris-hydrochloride, 5 mM NaF, 5 mM MgCl2, 10 mM Mercaptoethanol
PH: 7.1
Temperature: 37
- [galactokinase(Enzyme) wildtype]

D_Galactose + ATP -> ADP + alpha_D_Galactose_1_phosphate		 (( (Vmax * S) ) / (Km + S)) buffer: 0.03 M phosphate, 7.3 mg/ml BSA, 3 mM mercaptoethanol, 1.5 mM MgCl2, 3.75 mM NaF
PH: 7.3
Temperature: 25
- [galactokinase(Enzyme) wildtype]

ATP + D_Galactose -> alpha_D_Galactose_1_phosphate + ADP		 (( (Vmax * S) ) / (Km + S)) buffer: 0.03 M phosphate, 7.3 mg/ml BSA, 3 mM mercaptoethanol, 1.5 mM MgCl2, 3.75 mM NaF
PH: 7.3
Temperature: 25
- [galactokinase(Enzyme) wildtype]

D_Galactosamine + ATP -> ADP + D_Galactosamine_1_phosphate		 (( (Vmax * S) ) / (Km + S)) buffer: 0.03 M phosphate, 7.3 mg/ml BSA, 3 mM mercaptoethanol, 1.5 mM MgCl2, 3.75 mM NaF
PH: 7.3
Temperature: 25
- [galactokinase(Enzyme) wildtype]

ATP + 2_Deoxy_D_galactose -> ADP + 2_Deoxy_D_galactose_1_phosphate		 (( (Vmax * S) ) / (Km + S)) buffer: 0.03 M phosphate, 7.3 mg/ml BSA, 3 mM mercaptoethanol, 1.5 mM MgCl2, 3.75 mM NaF
PH: 7.3
Temperature: 25
uniprot:P51570 [galactokinase(Enzyme) mutant H44Y]

ATP + D_Galactose -> ADP + alpha_D_Galactose_1_phosphate		 (( (( (( (( (E * kcat) ) * B) ) / (KmB + B)) ) * A) ) / (KmA + A)) buffer: 20 mM Hepes, 150 mM NaCl, 5 mM MgCl2, 1 mM KCl, 10% (v/v) glycerol, 1 mM NADH, 1 mM dithiothreitol, 400 µM phosphoenolpyruvate, 7.5 U pyruvate kinase, 10 U lactate dehydrogenase
PH: 8
Temperature: 37
uniprot:P51570 [galactokinase(Enzyme) mutant R68C]

D_Galactose + ATP -> ADP + alpha_D_Galactose_1_phosphate		 (( (( (( (( (E * kcat) ) * B) ) / (KmB + B)) ) * A) ) / (KmA + A)) buffer: 20 mM Hepes, 150 mM NaCl, 5 mM MgCl2, 1 mM KCl, 10% (v/v) glycerol, 1 mM NADH, 1 mM dithiothreitol, 400 µM phosphoenolpyruvate, 7.5 U pyruvate kinase, 10 U lactate dehydrogenase
PH: 8
Temperature: 37
uniprot:P51570 [galactokinase(Enzyme) wildtype His-tagged]

ATP + D_Galactose -> alpha_D_Galactose_1_phosphate + ADP		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 20 mM Hepes, 150 mM NaCl, 5 mM MgCl2, 1 mM KCl, 10% (v/v) glycerol, 1 mM NADH, 1 mM dithiothreitol, 400 µM phosphoenolpyruvate, 7.5 U pyruvate kinase, 10 U lactate dehydrogenase
PH: 8
Temperature: 37
uniprot:P51570 [galactokinase(Enzyme) mutant G346S]

D_Galactose + ATP -> alpha_D_Galactose_1_phosphate + ADP		 (( (( (( (( (E * kcat) ) * B) ) / (KmB + B)) ) * A) ) / (KmA + A)) buffer: 20 mM Hepes, 150 mM NaCl, 5 mM MgCl2, 1 mM KCl, 10% (v/v) glycerol, 1 mM NADH, 1 mM dithiothreitol, 400 µM phosphoenolpyruvate, 7.5 U pyruvate kinase, 10 U lactate dehydrogenase
PH: 8
Temperature: 37
uniprot:P51570 [galactokinase(Enzyme) mutant G349S]

ATP + D_Galactose -> ADP + alpha_D_Galactose_1_phosphate		 (( (( (( (( (E * kcat) ) * B) ) / (KmB + B)) ) * A) ) / (KmA + A)) buffer: 20 mM Hepes, 150 mM NaCl, 5 mM MgCl2, 1 mM KCl, 10% (v/v) glycerol, 1 mM NADH, 1 mM dithiothreitol, 400 µM phosphoenolpyruvate, 7.5 U pyruvate kinase, 10 U lactate dehydrogenase
PH: 8
Temperature: 37
uniprot:P51570 [galactokinase(Enzyme) mutant A198V]

ATP + D_Galactose -> ADP + alpha_D_Galactose_1_phosphate		 (( (( (( (( (E * kcat) ) * B) ) / (KmB + B)) ) * A) ) / (KmA + A)) buffer: 20 mM Hepes, 150 mM NaCl, 5 mM MgCl2, 1 mM KCl, 10% (v/v) glycerol, 1 mM NADH, 1 mM dithiothreitol, 400 µM phosphoenolpyruvate, 7.5 U pyruvate kinase, 10 U lactate dehydrogenase
PH: 8
Temperature: 37
uniprot:P51570 [galactokinase(Enzyme) mutant G347S]

ATP + D_Galactose -> alpha_D_Galactose_1_phosphate + ADP		 (( (( (( (( (E * kcat) ) * B) ) / (KmB + B)) ) * A) ) / (KmA + A)) buffer: 20 mM Hepes, 150 mM NaCl, 5 mM MgCl2, 1 mM KCl, 10% (v/v) glycerol, 1 mM NADH, 1 mM dithiothreitol, 400 µM phosphoenolpyruvate, 7.5 U pyruvate kinase, 10 U lactate dehydrogenase
PH: 8
Temperature: 37
uniprot:P51570 [galactokinase(Enzyme) wildtype His-tagged]

D_Galactose + ATP -> alpha_D_Galactose_1_phosphate + ADP		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 20 mM Hepes, 150 mM NaCl, 5 mM MgCl2, 1 mM KCl, 10% (v/v) glycerol, 1 mM NADH, 1 mM dithiothreitol, 400 µM phosphoenolpyruvate, 7.5 U pyruvate kinase, 10 U lactate dehydrogenase
PH: 8
Temperature: 37
uniprot:P0A6T3 [galactokinase(Enzyme) wildtype N-His6]

D_Galactose + ATP -> ADP + alpha_D_Galactose_1_phosphate		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Sodium phosphate, 5 mM MgCl2
PH: 7.5
Temperature: 30
uniprot:P0A6T3 [galactokinase(Enzyme) wildtype N-His6]

D_Galactose + ATP -> alpha_D_Galactose_1_phosphate + ADP		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Sodium phosphate, 5 mM MgCl2
PH: 7.5
Temperature: 30
uniprot:P0A6T3 [galactokinase(Enzyme) wildtype N-His6]

D_Galactosamine + ATP -> D_Galactosamine_1_phosphate + ADP		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 50 mM Sodium phosphate, 5 mM MgCl2
PH: 7.5
Temperature: 30
uniprot:P0A6T3 [galactokinase(Enzyme) wildtype N-His6]

3_Deoxy_D_galactose + ATP -> ADP + 3_Deoxy_D_galactose_1_phosphate		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 50 mM Sodium phosphate, 5 mM MgCl2
PH: 7.5
Temperature: 30
uniprot:P0A6T3 [galactokinase(Enzyme) wildtype N-His6]

2_Deoxy_D_galactose + ATP -> 2_Deoxy_D_galactose_1_phosphate + ADP		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 50 mM Sodium phosphate, 5 mM MgCl2
PH: 7.5
Temperature: 30
uniprot:P0A6T3 [galactokinase(Enzyme) wildtype N-His6]

D_Fucose + ATP -> ADP + 6_Deoxy_D_galactose_1_phosphate		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 50 mM Sodium phosphate, 5 mM MgCl2
PH: 7.5
Temperature: 30
uniprot:P51570 [galactokinase(Enzyme) wildtype His-tagged]

ATP + D_Galactose -> ADP + alpha_D_Galactose_1_phosphate		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 20 mM HEPES, 150 mM NaCl, 5 mM MgCl2, 1 mM KCl, 10%(v/v) glycerol, 1 mM NADH, 1 mM DTT, 400 µM PEP, 50 U/ml pyruvate kinase, 66.7 U/ml lactate dehydrogenase
PH: 8
Temperature: 37
uniprot:P51570 [galactokinase(Enzyme) mutant E43A His-tagged]

D_Galactose + ATP -> alpha_D_Galactose_1_phosphate + ADP		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 20 mM HEPES, 150 mM NaCl, 5 mM MgCl2, 1 mM KCl, 10%(v/v) glycerol, 1 mM NADH, 1 mM DTT, 400 µM PEP, 50 U/ml pyruvate kinase, 66.7 U/ml lactate dehydrogenase
PH: 8
Temperature: 37
uniprot:P51570 [galactokinase(Enzyme) wildtype His-tagged]

D_Galactose + ATP -> ADP + alpha_D_Galactose_1_phosphate		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 20 mM HEPES, 150 mM NaCl, 5 mM MgCl2, 1 mM KCl, 10%(v/v) glycerol, 1 mM NADH, 1 mM DTT, 400 µM PEP, 50 U/ml pyruvate kinase, 66.7 U/ml lactate dehydrogenase
PH: 8
Temperature: 37
uniprot:P51570 [galactokinase(Enzyme) mutant E43G His-tagged]

D_Galactose + ATP -> alpha_D_Galactose_1_phosphate + ADP		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 20 mM HEPES, 150 mM NaCl, 5 mM MgCl2, 1 mM KCl, 10%(v/v) glycerol, 1 mM NADH, 1 mM DTT, 400 µM PEP, 50 U/ml pyruvate kinase, 66.7 U/ml lactate dehydrogenase
PH: 8
Temperature: 37
uniprot:P51570 [galactokinase(Enzyme) mutant E43G His-tagged]

D_Galactose + ATP -> ADP + alpha_D_Galactose_1_phosphate		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 20 mM HEPES, 150 mM NaCl, 5 mM MgCl2, 1 mM KCl, 10%(v/v) glycerol, 1 mM NADH, 1 mM DTT, 400 µM PEP, 50 U/ml pyruvate kinase, 66.7 U/ml lactate dehydrogenase
PH: 8
Temperature: 37
uniprot:P51570 [galactokinase(Enzyme) mutant E43A His-tagged]

ATP + D_Galactose -> alpha_D_Galactose_1_phosphate + ADP		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 20 mM HEPES, 150 mM NaCl, 5 mM MgCl2, 1 mM KCl, 10%(v/v) glycerol, 1 mM NADH, 1 mM DTT, 400 µM PEP, 50 U/ml pyruvate kinase, 66.7 U/ml lactate dehydrogenase
PH: 8
Temperature: 37
uniprot:P51570 [galactokinase(Enzyme) wildtype His-tagged]

2_Deoxy_D_galactose + ATP -> ADP + 2_Deoxy_D_galactose_1_phosphate		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 20 mM HEPES, 150 mM NaCl, 5 mM MgCl2, 1 mM KCl, 10%(v/v) glycerol, 1 mM NADH, 1 mM DTT, 400 µM PEP, 50 U/ml pyruvate kinase, 66.7 U/ml lactate dehydrogenase
PH: 8
Temperature: 37
uniprot:P51570 [galactokinase(Enzyme) wildtype His-tagged]

ATP + 2_Deoxy_D_galactose -> ADP + 2_Deoxy_D_galactose_1_phosphate		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 20 mM HEPES, 150 mM NaCl, 5 mM MgCl2, 1 mM KCl, 10%(v/v) glycerol, 1 mM NADH, 1 mM DTT, 400 µM PEP, 50 U/ml pyruvate kinase, 66.7 U/ml lactate dehydrogenase
PH: 8
Temperature: 37

Pathways

pathway id name
BioCyc:META_PWY-3821 D-galactose detoxification
BioCyc:META_PWY66-422 D-galactose degradation V (Leloir pathway)
BioCyc:META_PWY-6317 D-galactose degradation I (Leloir pathway)
BioCyc:ARA_PWY-7344 UDP-D-galactose biosynthesis
BioCyc:ARA_PWY-5114 UDP-sugars interconversion
BioCyc:ARA_PWY-3821 D-galactose degradation III
BioCyc:MTBH37RV_PWY-6317 D-galactose degradation I (Leloir pathway)
BioCyc:MOUSE_PWY-6317 galactose degradation I (Leloir pathway)
BioCyc:BSUB_PWY-6317 D-galactose degradation I (Leloir pathway)
BioCyc:ECOL316407_GALACTMETAB-PWY galactose degradation I (Leloir pathway)
BioCyc:HUMAN_PWY66-422 D-galactose degradation V (Leloir pathway)
BioCyc:YEAST_PWY66-422 D-galactose degradation V (Leloir pathway)
BioCyc:ARA_PWY-63 UDP-L-arabinose biosynthesis I (from UDP-xylose)
BioCyc:ECOO157_PWY-6317 galactose degradation I (Leloir pathway)
BioCyc:MOUSE_PWY3DJ-193 Leloir pathway
BioCyc:MOUSE_COLANSYN-PWY colanic acid building blocks biosynthesis
BioCyc:ARA_PWY-6527 stachyose degradation
BioCyc:MOUSE_GALACTMETAB-PWY UDP-galactose biosynthesis (salvage pathway from galactose using UDP-glucose)
BioCyc:ECO_GALACTMETAB-PWY galactose degradation I (Leloir pathway)
BioCyc:ARA_PWY-6317 D-galactose degradation I (Leloir pathway)
BioCyc:ECOL199310_PWY-6317 galactose degradation I (Leloir pathway)
PathBank:SMP0000841 Galactose Metabolism
PathBank:SMP0000903 Galactose Degradation/Leloir Pathway
PlantCyc:ARA_PWY-5114 UDP-sugars interconversion
PlantCyc:ARA_PWY-6527 stachyose degradation
PlantCyc:ARA_PWY-7344 UDP-α-D-galactose biosynthesis
PlantCyc:ARA_PWY-6317 D-galactose degradation I (Leloir pathway)
PlantCyc:PLANT_PWY-7344 UDP-α-D-galactose biosynthesis
PlantCyc:ARA_PWY-3821 D-galactose detoxification
PlantCyc:ARA_PWY-63 UDP-β-L-arabinose biosynthesis I (from UDP-α-D-xylose)
PlantCyc:PLANT_PWY-6317 D-galactose degradation I (Leloir pathway)
PlantCyc:PLANT_PWY-3821 D-galactose detoxification
Reactome:R-BTA-1430728 Metabolism
Reactome:R-MMU-1430728 Metabolism
Reactome:R-HSA-71387 Carbohydrate metabolism
Reactome:R-BTA-71387 Carbohydrate metabolism
Reactome:R-BTA-70370 Galactose catabolism
Reactome:R-HSA-70370 Galactose catabolism
Reactome:R-MMU-71387 Carbohydrate metabolism
Reactome:R-MMU-70370 Galactose catabolism
Reactome:R-HSA-1430728 Metabolism
PathBank:SMP0000496 Galactosemia III
PathBank:SMP0002319 Leloir Pathway
PathBank:SMP0002331 Amino Sugar and Nucleotide Sugar Metabolism
PathBank:SMP0000495 Galactosemia II (GALK)
PathBank:SMP0000010 Nucleotide Sugars Metabolism
PathBank:SMP0012059 D-Galactose Degradation (Leloir pathway)