EC: 2.7.1.29

glycerone kinase (ATP:glycerone phosphotransferase)

enzyme lambda metabolic reaction experiment
uniprot:P45510 [glycerone kinase(Enzyme) wildtype]

Glyceraldehyde + ATP -> Glyceraldehyde_3_phosphate + ADP		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 100 mM Hepes, 5 mM DTT, 5 mM NaF, 5 mM MgCl2, 1 mg/ml BSA, 10 µM EDTA, 7 units/ml lactate dehydrogenase, 0.4 mM NADH, 3 units/ml glycerol-3-phosphate dehydrogenase
PH: 7.5
Temperature: 30
uniprot:P45510 [glycerone kinase(Enzyme) wildtype]

Glyceraldehyde + ATP -> Glyceraldehyde_3_phosphate + ADP		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 100 mM Hepes, 5 mM DTT, 5 mM NaF, 5 mM MgCl2, 1 mg/ml BSA, 10 µM EDTA, 7 units/ml lactate dehydrogenase, 0.4 mM NADH, 3 units/ml glycerol-3-phosphate dehydrogenase
PH: 7.5
Temperature: 30
uniprot:P45510 [glycerone kinase(Enzyme) wildtype]

ATP + Glyceraldehyde -> Glyceraldehyde_3_phosphate + ADP		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 100 mM Hepes, 5 mM DTT, 5 mM NaF, 5 mM MgCl2, 1 mg/ml BSA, 10 µM EDTA, 7 units/ml lactate dehydrogenase, 0.4 mM NADH, 3 units/ml glycerol-3-phosphate dehydrogenase
PH: 7.5
Temperature: 30
uniprot:P45510 [glycerone kinase(Enzyme) wildtype]

Glyceraldehyde + ATP -> Glyceraldehyde_3_phosphate + ADP		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 100 mM Hepes, 5 mM DTT, 5 mM NaF, 5 mM MgCl2, 1 mg/ml BSA, 10 µM EDTA, 7 units/ml lactate dehydrogenase, 0.4 mM NADH, 3 units/ml glycerol-3-phosphate dehydrogenase
PH: 7.5
Temperature: 30
uniprot:P45510 [glycerone kinase(Enzyme) wildtype]

ATP + Glyceraldehyde -> ADP + Glyceraldehyde_3_phosphate		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 100 mM Hepes, 5 mM DTT, 5 mM NaF, 5 mM MgCl2, 1 mg/ml BSA, 10 µM EDTA, 7 units/ml lactate dehydrogenase, 0.4 mM NADH, 3 units/ml glycerol-3-phosphate dehydrogenase
PH: 7.5
Temperature: 30
uniprot:P45510 [glycerone kinase(Enzyme) wildtype]

ATP + Glyceraldehyde -> ADP + Glyceraldehyde_3_phosphate		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 100 mM Hepes, 5 mM DTT, 5 mM NaF, 5 mM MgCl2, 1 mg/ml BSA, 10 µM EDTA, 7 units/ml lactate dehydrogenase, 0.4 mM NADH, 3 units/ml glycerol-3-phosphate dehydrogenase
PH: 7.5
Temperature: 30
uniprot:P45510 [glycerone kinase(Enzyme) wildtype]

ATP + Dihydroxyacetone -> Glycerone_phosphate + ADP		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM triethanolamine-hydrochloride, 1 mM MgCl2, 0.1 mM NADH, 2 µg/ml G3P dehydrogenase from rabbit muscle, 10 mM alpha,alpha-dipyridyl
PH: 7
Temperature: 30
- [glycerone kinase(Enzyme) wildtype]

Dihydroxyacetone + ATP -> Glycerone_phosphate + ADP		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM triethanolamine-hydrochloride, 1 mM MgCl2, 0.1 mM NADH, 2 µg/ml Glycerol 3-phosphate dehydrogenase from rabbit muscle, 10 mM alpha,alpha-dipyridyl
PH: 7
Temperature: 30
uniprot:P45510 [glycerone kinase(Enzyme) wildtype]

ATP + Dihydroxyacetone -> Glycerone_phosphate + ADP		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM triethanolamine-hydrochloride, 1 mM MgCl2, 0.1 mM NADH, 2 µg/ml G3P dehydrogenase from rabbit muscle, 10 mM alpha,alpha-dipyridyl
PH: 7
Temperature: 30

Pathways

pathway id name
BioCyc:META_P185-PWY formaldehyde assimilation III (dihydroxyacetone cycle)
BioCyc:META_GOLPDLCAT-PWY superpathway of glycerol degradation to 1,3-propanediol
BioCyc:META_PWY-6131 glycerol degradation II
BioCyc:HUMAN_PWY66-373 sucrose degradation
Reactome:R-HSA-9679506 SARS-CoV Infections
Reactome:R-HSA-9694516 SARS-CoV-2 Infection
Reactome:R-HSA-9692914 SARS-CoV-1-host interactions
Reactome:R-HSA-9705671 SARS-CoV-2 activates/modulates innate and adaptive immune responses
Reactome:R-HSA-9692916 SARS-CoV-1 activates/modulates innate immune responses
Reactome:R-HSA-9678108 SARS-CoV-1 Infection
Reactome:R-HSA-9824446 Viral Infection Pathways
Reactome:R-HSA-9705683 SARS-CoV-2-host interactions
Reactome:R-DDI-70350 Fructose catabolism
Reactome:R-HSA-1643685 Disease
Reactome:R-MMU-1430728 Metabolism
Reactome:R-RNO-70350 Fructose catabolism
Reactome:R-SCE-71387 Carbohydrate metabolism
Reactome:R-SCE-5652084 Fructose metabolism
Reactome:R-SPO-1430728 Metabolism
Reactome:R-DDI-71387 Carbohydrate metabolism
Reactome:R-HSA-71387 Carbohydrate metabolism
Reactome:R-HSA-70350 Fructose catabolism
Reactome:R-HSA-168928 DDX58/IFIH1-mediated induction of interferon-alpha/beta
Reactome:R-MMU-70350 Fructose catabolism
Reactome:R-SCE-1430728 Metabolism
Reactome:R-SPO-70350 Fructose catabolism
Reactome:R-HSA-5652084 Fructose metabolism
Reactome:R-HSA-168256 Immune System
Reactome:R-HSA-168249 Innate Immune System
Reactome:R-MMU-71387 Carbohydrate metabolism
Reactome:R-MMU-5652084 Fructose metabolism
Reactome:R-RNO-1430728 Metabolism
Reactome:R-SPO-71387 Carbohydrate metabolism
Reactome:R-SPO-5652084 Fructose metabolism
Reactome:R-DDI-1430728 Metabolism
Reactome:R-DDI-5652084 Fructose metabolism
Reactome:R-HSA-1430728 Metabolism
Reactome:R-HSA-5663205 Infectious disease
Reactome:R-RNO-71387 Carbohydrate metabolism
Reactome:R-RNO-5652084 Fructose metabolism
Reactome:R-SCE-70350 Fructose catabolism
PathBank:SMP0002328 Glycerol Metabolism