EC: 1.14.18.9

4alpha-methylsterol monooxygenase (4,4-dimethyl-5alpha-cholest-7-en-3beta-ol,ferrocytochrome-b5:oxygen oxidoreductase (C4alpha-methyl-hydroxylating))

enzyme lambda metabolic reaction experiment
uniprot:O35532 [methylsterol monooxygenase(Enzyme) wildtype]

H+ + NADH + O2 + 5alpha_Cholest_7_ene_3_one_4alpha_carbaldehyde -> 5alpha_Cholest_7_en_3_one + NAD+ + H2O + CO2		 (( (Vmax * A) ) / (Km + A)) buffer: 0.1 M Potassium phosphate, 10 mM beta-Hydroxybutyrate, 0.31 units/ml beta-Hydroxybutyrate dehydrogenase
PH: 7.4
Temperature: 37
uniprot:O35532 [methylsterol monooxygenase(Enzyme) wildtype]

4alpha_Hydroxymethyl_4beta_methyl_5alpha_cholest_7_en_3beta_ol + H+ + NADPH + O2 -> NADP+ + 3beta_Hydroxy_4beta_methyl_5alpha_cholest_7_ene_4alpha_carbaldehyde + H2O		 (( (Vmax * A) ) / (Km + A)) buffer: 0.1 M Phosphate, 5.8 mM Isocitrate, 0.6 mM NADP+, 0.25 mg/ml Isocitric dehydrogenase, 2.3 mM Pyruvate, 1.3 mM NAD+, 0.05 mg/ml Lactic dehydrogenase
PH: 7.4
Temperature: 37
uniprot:O35532 [methylsterol monooxygenase(Enzyme) wildtype]

O2 + 4alpha_Hydroxymethyl_5alpha_cholest_7_en_3beta_ol + NADPH + H+ -> 3beta_Hydroxy_5alpha_cholest_7_ene_4alpha_carbaldehyde + NADP+ + H2O		 (( (Vmax * A) ) / (Km + A)) buffer: 0.1 M Phosphate, 5.8 mM Isocitrate, 0.6 mM NADP+, 0.25 mg/ml Isocitric dehydrogenase, 2.3 mM Pyruvate, 1.3 mM NAD+, 0.05 mg/ml Lactic dehydrogenase
PH: 7.4
Temperature: 37

Pathways

pathway id name
BioCyc:MOUSE_PWY66-3 cholesterol biosynthesis II (via 24,25-dihydrolanosterol)
BioCyc:HUMAN_PWY66-5 superpathway of cholesterol biosynthesis
BioCyc:HUMAN_PWY66-341 cholesterol biosynthesis I
BioCyc:ARA_PWY-2541 plant sterol biosynthesis
BioCyc:MOUSE_PWY66-341 cholesterol biosynthesis I
BioCyc:HUMAN_PWY66-3 cholesterol biosynthesis II (via 24,25-dihydrolanosterol)
BioCyc:HUMAN_PWY-6074 zymosterol biosynthesis
BioCyc:ARA_PWY-6663 plant sterol biosynthesis II
BioCyc:HUMAN_PWY66-4 cholesterol biosynthesis III (via desmosterol)
BioCyc:MOUSE_PWY66-4 cholesterol biosynthesis III (via desmosterol)
PlantCyc:ARA_PWY-2541 phytosterol biosynthesis (plants)
PlantCyc:PLANT_PWY-2541 phytosterol biosynthesis (plants)
PlantCyc:ARA_PWY-6663 plant sterol biosynthesis II
Reactome:R-HSA-6807047 Cholesterol biosynthesis via desmosterol (Bloch pathway)
Reactome:R-MMU-1430728 Metabolism
Reactome:R-MMU-556833 Metabolism of lipids
Reactome:R-MMU-8957322 Metabolism of steroids
Reactome:R-DRE-6807047 Cholesterol biosynthesis via desmosterol (Bloch pathway)
Reactome:R-HSA-556833 Metabolism of lipids
Reactome:R-HSA-191273 Cholesterol biosynthesis
Reactome:R-RNO-6807047 Cholesterol biosynthesis via desmosterol (Bloch pathway)
Reactome:R-DRE-1430728 Metabolism
Reactome:R-DRE-556833 Metabolism of lipids
Reactome:R-DRE-8957322 Metabolism of steroids
Reactome:R-HSA-6807062 Zymostenol biosynthesis via lathosterol (Kandutsch-Russell pathway)
Reactome:R-MMU-191273 Cholesterol biosynthesis
Reactome:R-RNO-1430728 Metabolism
Reactome:R-RNO-556833 Metabolism of lipids
Reactome:R-RNO-8957322 Metabolism of steroids
Reactome:R-DRE-191273 Cholesterol biosynthesis
Reactome:R-HSA-1430728 Metabolism
Reactome:R-HSA-8957322 Metabolism of steroids
Reactome:R-MMU-6807047 Cholesterol biosynthesis via desmosterol (Bloch pathway)
Reactome:R-RNO-191273 Cholesterol biosynthesis
WikiPathways:WP4346 Cholesterol metabolism with Bloch and Kandutsch-Russell pathways
PathBank:SMP0120794 Mevalonic Aciduria
PathBank:SMP0121057 Bloch Pathway (Cholesterol Biosynthesis)
PathBank:SMP0000023 Steroid Biosynthesis
PathBank:SMP0000387 CHILD Syndrome
PathBank:SMP0000112 Risedronate Action Pathway
PathBank:SMP0000508 Cholesteryl Ester Storage Disease
PathBank:SMP0087326 Steroid Biosynthesis
PathBank:SMP0120685 Chondrodysplasia Punctata II, X-Linked Dominant (CDPX2)
PathBank:SMP0120769 Hypercholesterolemia
PathBank:SMP0120793 Hyper-IgD Syndrome
PathBank:SMP0000389 Smith-Lemli-Opitz Syndrome (SLOS)
PathBank:SMP0000319 Lysosomal Acid Lipase Deficiency (Wolman Disease)
PathBank:SMP0000082 Simvastatin Action Pathway
PathBank:SMP0000111 Cerivastatin Action Pathway
PathBank:SMP0000131 Atorvastatin Action Pathway
PathBank:SMP0000511 Wolman Disease
PathBank:SMP0120684 CHILD Syndrome
PathBank:SMP0120692 Desmosterolosis
PathBank:SMP0120732 Lysosomal Acid Lipase Deficiency (Wolman Disease)
PathBank:SMP0120792 Cholesteryl Ester Storage Disease
PathBank:SMP0121060 Kandutsch-Russell Pathway (Cholesterol Biosynthesis)
PathBank:SMP0000386 Desmosterolosis
PathBank:SMP0000089 Pravastatin Action Pathway
PathBank:SMP0000095 Alendronate Action Pathway
PathBank:SMP0000099 Lovastatin Action Pathway
PathBank:SMP0000117 Pamidronate Action Pathway
PathBank:SMP0000509 Hyper-IgD Syndrome
PathBank:SMP0120735 Smith-Lemli-Opitz Syndrome (SLOS)
PathBank:SMP0120795 Wolman Disease
PathBank:SMP0121236 Steroid Biosynthesis
PathBank:SMP0000388 Chondrodysplasia Punctata II, X-Linked Dominant (CDPX2)
PathBank:SMP0000079 Ibandronate Action Pathway
PathBank:SMP0000092 Rosuvastatin Action Pathway
PathBank:SMP0000209 Hypercholesterolemia
PathBank:SMP0000107 Zoledronate Action Pathway
PathBank:SMP0000119 Fluvastatin Action Pathway
PathBank:SMP0000510 Mevalonic Aciduria