Cholesterol biosynthesis via desmosterol (Bloch pathway)

Mitsche MA, McDonald JG, Hobbs HH, Cohen JC. Flux analysis of cholesterol biosynthesis in vivo reveals multiple tissue and cell-type specific pathways. Elife. 2015 Jun 26;4():e07999. PMID: 26114596; PMCID: PMC4501332.

Metabolites

4,4-Dimethyl-5alpha-cholesta-8,14,24-trien-3beta-ol

Formula: C29H46O (410.3548466)

CAS ID: 64284-64-6

5alpha-Cholesta-7,24-dien-3beta-ol

Formula: C27H44O (384.3391974)

CAS ID: 651-54-7

7-Dehydrodesmosterol

Formula: C27H42O (382.3235482)

CAS ID: 1715-86-2

Cholesterol

Formula: C27H46O (386.3548466)

CAS ID: 57-88-5

CO2

Formula: CO2 (43.98983)

CAS ID: 124-38-9

Desmosterol

Formula: C27H44O (384.3391974)

CAS ID: 313-04-2

H+

Formula: H (1.0078246)

CAS ID: 12408-02-5

H2O

Formula: H2O (18.0105642)

CAS ID: 7732-18-5

Lanosterol

Formula: C30H50O (426.386145)

CAS ID: 79-63-0

NADP+

Formula: C21H29N7O17P3 (744.0832754)

CAS ID: 53-59-8

NADPH

Formula: C21H30N7O17P3 (745.0911)

CAS ID: 2646-71-1

Oxygen

Formula: O2 (31.98983)

CAS ID: 7782-44-7

Zymosterol

Formula: C27H44O (384.3391974)

CAS ID: 128-33-6

Zymosterone

Formula: C27H42O (382.3235482)

CAS ID: 27192-37-6

formate

Formula: CHO2 (44.997654600000004)

CAS ID: 71-47-6

NADPH(4-)

Formula: C21H26N7O17P3 (741.0598016)

CAS ID: 53-57-6

NADH(2-)

Formula: C21H27N7O14P2 (663.1091182000001)

CAS ID:

NAD(1-)

Formula: C21H26N7O14P2 (662.1012936000001)

CAS ID: 53-84-9

NADP(3-)

Formula: C21H25N7O17P3 (740.051977)

CAS ID: 53-59-8



Enzyme

EC Number name full name note
1.14.14.154 sterol 14alpha-demethylase sterol,[reduced NADPH-hemoprotein reductase]:oxygen oxidoreductase (14-methyl cleaving)
1.14.18.9 4alpha-methylsterol monooxygenase 4,4-dimethyl-5alpha-cholest-7-en-3beta-ol,ferrocytochrome-b5:oxygen oxidoreductase (C4alpha-methyl-hydroxylating)
1.3.1.21 7-dehydrocholesterol reductase cholesterol:NADP+ DELTA7-oxidoreductase


Proteins

Protein ID name full name