EC: 1.14.14.25

cholesterol 24-hydroxylase (cholesterol,NADPH-hemoprotein reductase:oxygen oxidoreductase (24-hydroxylating))

enzyme lambda metabolic reaction experiment
uniprot:Q9Y6A2 [cholesterol 24-hydroxylase(Enzyme) wildtype CYP46A1]

O2 + H+ + NADPH + Phenacetin -> Ethanol + Acetaminophen + NADP+ + H2O		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 0.2% sodium cholate, 40 mM potassium phosphate, 10 µM NADPH-cytochrome P450 reductase
PH: 7.2
Temperature: 37
uniprot:Q9Y6A2 [cholesterol 24-hydroxylase(Enzyme) wildtype CYP46A1]

Testosterone + NADPH + H+ + O2 -> H2O + 16beta_Hydroxytestosterone + NADP+		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 0.2% sodium cholate, 40 mM potassium phosphate, 10 µM NADPH-cytochrome P450 reductase
PH: 7.2
Temperature: 37
uniprot:Q9Y6A2 [cholesterol 24-hydroxylase(Enzyme) wildtype CYP46A1]

NADPH + H+ + O2 + Testosterone -> NADP+ + 2_Hydroxytestosterone + H2O		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 0.2% sodium cholate, 40 mM potassium phosphate, 10 µM NADPH-cytochrome P450 reductase
PH: 7.2
Temperature: 37
uniprot:Q9Y6A2 [cholesterol 24-hydroxylase(Enzyme) wildtype CYP46A1]

Testosterone + H+ + NADPH + O2 -> H2O + 6beta_Hydroxytestosterone + NADP+		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 0.2% sodium cholate, 40 mM potassium phosphate, 10 µM NADPH-cytochrome P450 reductase
PH: 7.2
Temperature: 37
uniprot:Q9Y6A2 [cholesterol 24-hydroxylase(Enzyme) wildtype CYP46A1]

O2 + Cholesterol + NADPH + H+ -> 24(S)_Hydroxycholesterol + H2O + NADP+		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 0.2% sodium cholate, 40 mM potassium phosphate, 10 µM NADPH-cytochrome P450 reductase
PH: 7.2
Temperature: 37
uniprot:Q9Y6A2 [cholesterol 24-hydroxylase(Enzyme) wildtype CYP46A1]

NADPH + 24(S)_Hydroxycholesterol + O2 + H+ -> H2O + Dihydroxycholesterol + NADP+		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 0.2% sodium cholate, 40 mM potassium phosphate, 2 µM NADPH-cytochrome P450 reductase
PH: 7.2
Temperature: 37
uniprot:Q9Y6A2 [cholesterol 24-hydroxylase(Enzyme) wildtype CYP46A1]

Progesterone + O2 + NADPH + H+ -> NADP+ + H2O + 17alpha_Hydroxyprogesterone		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 0.2% sodium cholate, 40 mM potassium phosphate, 10 µM NADPH-cytochrome P450 reductase
PH: 7.2
Temperature: 37
uniprot:Q9Y6A2 [cholesterol 24-hydroxylase(Enzyme) wildtype CYP46A1]

O2 + H+ + Cortisol + NADPH -> NADP+ + H2O + Unknown		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 0.2% sodium cholate, 40 mM potassium phosphate, 10 µM NADPH-cytochrome P450 reductase
PH: 7.2
Temperature: 37
uniprot:Q9Y6A2 [cholesterol 24-hydroxylase(Enzyme) wildtype CYP46A1]

H+ + NADPH + Diclofenac + O2 -> 4'_Hydroxydiclofenac + H2O + NADP+		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 0.2% sodium cholate, 40 mM potassium phosphate, 10 µM NADPH-cytochrome P450 reductase
PH: 7.2
Temperature: 37
uniprot:Q9Y6A2 [cholesterol 24-hydroxylase(Enzyme) wildtype CYP46A1]

H+ + O2 + Dextromethorphan + NADPH -> Dextrorphan + NADP+ + H2O		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 0.2% sodium cholate, 40 mM potassium phosphate, 10 µM NADPH-cytochrome P450 reductase
PH: 7.2
Temperature: 37
uniprot:Q9Y6A2 [cholesterol 24-hydroxylase(Enzyme) wildtype CYP46A1]

O2 + NADPH + Dextromethorphan + H+ -> NADP+ + H2O + 3_Methoxydextrorphanin		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 0.2% sodium cholate, 40 mM potassium phosphate, 10 µM NADPH-cytochrome P450 reductase
PH: 7.2
Temperature: 37
uniprot:Q9Y6A2 [cholesterol 24-hydroxylase(Enzyme) wildtype CYP46A1]

H+ + NADPH + O2 + Bufuralol -> NADP+ + 1_Hydroxy_bufuralol + H2O		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 0.2% sodium cholate, 40 mM potassium phosphate, 10 µM NADPH-cytochrome P450 reductase
PH: 7.2
Temperature: 37

Pathways

pathway id name
Reactome:R-HSA-194068 Bile acid and bile salt metabolism
Reactome:R-HSA-211859 Biological oxidations
Reactome:R-MMU-1430728 Metabolism
Reactome:R-MMU-556833 Metabolism of lipids
Reactome:R-MMU-8957322 Metabolism of steroids
Reactome:R-MMU-193775 Synthesis of bile acids and bile salts via 24-hydroxycholesterol
Reactome:R-MMU-211859 Biological oxidations
Reactome:R-HSA-556833 Metabolism of lipids
Reactome:R-HSA-193775 Synthesis of bile acids and bile salts via 24-hydroxycholesterol
Reactome:R-HSA-211976 Endogenous sterols
Reactome:R-MMU-211976 Endogenous sterols
Reactome:R-HSA-192105 Synthesis of bile acids and bile salts
Reactome:R-HSA-211945 Phase I - Functionalization of compounds
Reactome:R-MMU-194068 Bile acid and bile salt metabolism
Reactome:R-MMU-211945 Phase I - Functionalization of compounds
Reactome:R-HSA-1430728 Metabolism
Reactome:R-HSA-8957322 Metabolism of steroids
Reactome:R-HSA-211897 Cytochrome P450 - arranged by substrate type
Reactome:R-MMU-192105 Synthesis of bile acids and bile salts
Reactome:R-MMU-211897 Cytochrome P450 - arranged by substrate type
WikiPathways:WP4545 Oxysterols derived from cholesterol
PathBank:SMP0120468 Congenital Bile Acid Synthesis Defect Type III
PathBank:SMP0000318 Congenital Bile Acid Synthesis Defect Type III
PathBank:SMP0120463 Cerebrotendinous Xanthomatosis (CTX)
PathBank:SMP0120467 Congenital Bile Acid Synthesis Defect Type II
PathBank:SMP0120495 Zellweger Syndrome
PathBank:SMP0000035 Bile Acid Biosynthesis
PathBank:SMP0000314 Congenital Bile Acid Synthesis Defect Type II
PathBank:SMP0000315 Cerebrotendinous Xanthomatosis (CTX)
PathBank:SMP0120652 27-Hydroxylase Deficiency
PathBank:SMP0120477 Familial Hypercholanemia (FHCA)
PathBank:SMP0000317 Familial Hypercholanemia (FHCA)
PathBank:SMP0000316 Zellweger Syndrome
PathBank:SMP0000720 27-Hydroxylase Deficiency
PathBank:SMP0063601 Bile Acid Biosynthesis