L-methionine salvage cycle I (bacteria and plants)

Cobzaru C, Ganas P, Mihasan M, Schleberger P, Brandsch R. Homologous gene clusters of nicotine catabolism, including a new ?-amidase for a-ketoglutaramate, in species of three genera of Gram-positive bacteria. Res Microbiol. 2011 Apr;162(3):285–91. doi: 10.1016/j.resmic.2011.01.001. PMID: 21288482.; Albers E. Metabolic characteristics and importance of the universal methionine salvage pathway recycling methionine from 5'-methylthioadenosine. IUBMB Life. 2009 Dec;61(12):1132–42. doi: 10.1002/iub.278. PMID: 19946895.

Metabolites

5'-Methylthioadenosine

Formula: C11H15N5O3S (297.089556)

CAS ID: 2457-80-9

Adenine

Formula: C5H5N5 (135.054493)

CAS ID: 73-24-5

CO2

Formula: CO2 (43.98983)

CAS ID: 124-38-9

Diphosphate

Formula: H4P2O7 (177.9432294)

CAS ID: 2466-09-3

Fe2+

Formula: Fe (55.934939)

CAS ID: 15438-31-0

H+

Formula: H (1.0078246)

CAS ID: 12408-02-5

H2O

Formula: H2O (18.0105642)

CAS ID: 7732-18-5

Magnesium cation

Formula: Mg (23.98505)

CAS ID: 22537-22-0

Oxygen

Formula: O2 (31.98983)

CAS ID: 7782-44-7

Potassium cation

Formula: K (38.963708)

CAS ID: 24203-36-9

formate

Formula: CHO2 (44.997654600000004)

CAS ID: 71-47-6

2-oxoglutarate(2-)

Formula: C5H4O5 (144.00587339999998)

CAS ID: 64-15-3

2-oxoglutaramate

Formula: C5H6NO4 (144.0296816)

CAS ID: 18465-19-5

ATP(4-)

Formula: C10H12N5O13P3 (502.9644492)

CAS ID:

ADP(3-)

Formula: C10H12N5O10P2 (424.0059412)

CAS ID:



Enzyme

EC Number name full name note
1.13.11.53 acireductone dioxygenase (Ni2+-requiring) 1,2-dihydroxy-5-(methylthio)pent-1-en-3-one:oxygen oxidoreductase (formate- and CO-forming)
1.13.11.54 acireductone dioxygenase [iron(II)-requiring] 1,2-dihydroxy-5-(methylthio)pent-1-en-3-one:oxygen oxidoreductase (formate-forming)
2.5.1.6 methionine adenosyltransferase ATP:L-methionine S-adenosyltransferase
2.6.1.117 L-glutamine-4-(methylsulfanyl)-2-oxobutanoate aminotransferase L-glutamine:4-(methylsulfanyl)-2-oxobutanoate aminotransferase
2.7.1.100 S-methyl-5-thioribose kinase ATP:S-methyl-5-thio-D-ribose 1-phosphotransferase
3.1.3.77 acireductone synthase 5-(methylthio)-2,3-dioxopentyl-phosphate phosphohydrolase (isomerizing)
3.1.3.87 2-hydroxy-3-keto-5-methylthiopentenyl-1-phosphate phosphatase 2-hydroxy-5-(methylsulfanyl)-3-oxopent-1-en-1-yl phosphate phosphohydrolase
3.2.2.9 adenosylhomocysteine nucleosidase S-adenosyl-L-homocysteine homocysteinylribohydrolase
3.5.1.111 2-oxoglutaramate amidase 5-amino-2,5-dioxopentanoate amidohydrolase
4.1.1.50 adenosylmethionine decarboxylase S-adenosyl-L-methionine carboxy-lyase [(5-deoxy-5-adenosyl)(3-aminopropyl)methylsulfonium-salt-forming]
4.2.1.109 methylthioribulose 1-phosphate dehydratase S-methyl-5-thio-D-ribulose-1-phosphate 4-hydro-lyase [5-(methylthio)-2,3-dioxopentyl-phosphate-forming]
5.3.1.23 S-methyl-5-thioribose-1-phosphate isomerase S-methyl-5-thio-alpha-D-ribose-1-phosphate aldose-ketose-isomerase
5.3.2.5 2,3-diketo-5-methylthiopentyl-1-phosphate enolase 2,3-diketo-5-methylthiopentyl-1-phosphate keto-enol-isomerase


Proteins

Protein ID name full name
B5XZW2 mtnA Methylthioribose-1-phosphate isomerase
O31664 mtnU 2-oxoglutaramate amidase
O31665 mtnE L-glutamine--4-(methylsulfanyl)-2-oxobutanoate aminotransferase
O31666 mtnW 2,3-diketo-5-methylthiopentyl-1-phosphate enolase
O31667 mtnX 2-hydroxy-3-keto-5-methylthiopentenyl-1-phosphate phosphatase
O31668 mtnB Methylthioribulose-1-phosphate dehydratase
O32028 mtnN 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase
O34426 speH S-adenosylmethionine decarboxylase proenzyme
P54419 metK S-adenosylmethionine synthase
Q48389 mtnC Enolase-phosphatase E1
Q9F0P1 mtnK Methylthioribose kinase
Q9KPI8 mtnN 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase
Q9R4A1 mtnN 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase
Q9ZFE7 mtnD Acireductone dioxygenase