EC: 2.7.1.71

shikimate kinase (ATP:shikimate 3-phosphotransferase)

enzyme lambda metabolic reaction experiment
uniprot:P10880 [shikimate kinase(Enzyme) wildtype]

Shikimate + ATP -> Shikimate_3_phosphate + ADP		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM Triethanolamine hydrochloride/KOH, 50 mM KCl, 5 mM MgCl2, 1 mM Phosphoenolpyruvate, 0.1 mM NADH, 3 units/ml Pyruvate kinase, 2.5 units/ml Lactate dehydrogenase
PH: 7
Temperature: 25
uniprot:P10880 [shikimate kinase(Enzyme) mutant C162S]

Shikimate + ATP -> Shikimate_3_phosphate + ADP		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM Triethanolamine hydrochloride/KOH, 50 mM KCl, 5 mM MgCl2, 1 mM Phosphoenolpyruvate, 0.1 mM NADH, 3 units/ml Pyruvate kinase, 2.5 units/ml Lactate dehydrogenase
PH: 7
Temperature: 25
uniprot:P10880 [shikimate kinase(Enzyme) mutant C13S]

Shikimate + ATP -> ADP + Shikimate_3_phosphate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM Triethanolamine hydrochloride/KOH, 50 mM KCl, 5 mM MgCl2, 1 mM Phosphoenolpyruvate, 0.1 mM NADH, 3 units/ml Pyruvate kinase, 2.5 units/ml Lactate dehydrogenase
PH: 7
Temperature: 25
uniprot:P10880 [shikimate kinase(Enzyme) mutant C13S]

ATP + Shikimate -> Shikimate_3_phosphate + ADP		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM Triethanolamine hydrochloride/KOH, 50 mM KCl, 5 mM MgCl2, 1 mM Phosphoenolpyruvate, 0.1 mM NADH, 3 units/ml Pyruvate kinase, 2.5 units/ml Lactate dehydrogenase
PH: 7
Temperature: 25
uniprot:P10880 [shikimate kinase(Enzyme) wildtype]

Shikimate + ATP -> Shikimate_3_phosphate + ADP		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM Triethanolamine hydrochloride/KOH, 50 mM KCl, 5 mM MgCl2, 1 mM Phosphoenolpyruvate, 0.1 mM NADH, 3 units/ml Pyruvate kinase, 2.5 units/ml Lactate dehydrogenase
PH: 7
Temperature: 25
uniprot:P10880 [shikimate kinase(Enzyme) mutant C162S]

Shikimate + ATP -> Shikimate_3_phosphate + ADP		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM Triethanolamine hydrochloride/KOH, 50 mM KCl, 5 mM MgCl2, 1 mM Phosphoenolpyruvate, 0.1 mM NADH, 3 units/ml Pyruvate kinase, 2.5 units/ml Lactate dehydrogenase
PH: 7
Temperature: 25
uniprot:P0A4Z2 [shikimate kinase(Enzyme) wildtype]

ATP + Shikimate -> ADP + Shikimate_3_phosphate		 (( (( (( (kcat * E) ) * A) ) * B) ) / (( (( (( (KiA * KmB) ) + (KmA * B)) ) + (KmB * A)) ) + (A * B))) buffer: 100 mM Tris-HCl, 100 mM KCl, 5 mM MgCl2, 1.5 mM Phosphoenolpyruvate, 0.2 mM NADH, 40 units/ml Pyruvate kinase, 85 units/ml Lactate dehydrogenase
PH: 7.7
Temperature: 25
uniprot:P56073 [shikimate kinase(Enzyme) wildtype]

ATP + Shikimate -> Shikimate_3_phosphate + ADP		 (( (Vmax * S) ) / (Km + S)) buffer: 100 mM Tris-HCl-KOH, 50 mM KCl, 5 mM MgCl2, 1 mM phosphoenolpyruvate, 0.1 mM NADH, 2.5 U/ml pyruvate kinase, 2.7 U/ml lactate dehydrogenase
PH: 7.5
Temperature: 25
uniprot:P56073 [shikimate kinase(Enzyme) wildtype]

ATP + Shikimate -> Shikimate_3_phosphate + ADP		 (( (Vmax * S) ) / (Km + S)) buffer: 100 mM Tris-HCl-KOH, 50 mM KCl, 5 mM MgCl2, 1 mM phosphoenolpyruvate, 0.1 mM NADH, 2.5 U/ml pyruvate kinase, 2.7 U/ml lactate dehydrogenase
PH: 7.5
Temperature: 25
uniprot:Q58835 [shikimate kinase(Enzyme) wildtype His6-tagged]

ATP + Shikimate -> ADP + Shikimate_3_phosphate		 (( (( (( (E * kcat) ) * A) ) * B) ) / (( (( (KmA * B) ) + (KmB * A)) ) + (A * B))) buffer: 100 mM HEPES, 20 mM KCl, 10 mM MgCl2, 2 mM Phosphoenolpyruvate, 0.3 mM NADH, 0.01 U/µl Lactate dehydrogenase, 0.005 U/µl Pyruvate kinase
PH: 8
Temperature: 37
uniprot:Q5GA17 [3-Methoxy-4-{[2-({2-methoxy-4-[(4-oxo-2-thioxo-1,3-thiazolidin-5-ylidene)methyl]phenoxy}methyl)benzyl]oxy}benzaldehyde]

Shikimate + ATP -> Shikimate_3_phosphate + ADP		 (( (Vmax * S) ) / (( (S * (1 + (I / alphaKi))) ) + (Km * (1 + (I / Ki))))) buffer: 100 mM Tris-HCl, 50 mM KCl, 5 mM MgCl2, 2 mM phosphoenolpyruvate, 0.7 mM NADH, 3 U/ml Pyruvate kinase, 2.5 U/ml Lactate dehydrogenase, 0.1% (v/v) dimethylsulfoxide
PH: 8
Temperature: 25
uniprot:Q5GA17 [3-Methoxy-4-{[2-({2-methoxy-4-[(4-oxo-2-thioxo-1,3-thiazolidin-5-ylidene)methyl]phenoxy}methyl)benzyl]oxy}benzaldehyde]

ATP + Shikimate -> Shikimate_3_phosphate + ADP		 (( (Vmax * S) ) / (( (S * (1 + (I / alphaKi))) ) + (Km * (1 + (I / Ki))))) buffer: 100 mM Tris-HCl, 50 mM KCl, 5 mM MgCl2, 2 mM phosphoenolpyruvate, 0.7 mM NADH, 3 U/ml Pyruvate kinase, 2.5 U/ml Lactate dehydrogenase, 0.1% (v/v) dimethylsulfoxide
PH: 8
Temperature: 25
uniprot:Q5GA17 [5-Bromo-2-(5-{[1-(3,4-dichlorophenyl)-3,5-dioxo-4-pyrazolidinylidene]methyl}-2-furyl)benzoic acid]

ATP + Shikimate -> Shikimate_3_phosphate + ADP		 (( (Vmax * S) ) / (( (S * (1 + (I / alphaKi))) ) + (Km * (1 + (I / Ki))))) buffer: 100 mM Tris-HCl, 50 mM KCl, 5 mM MgCl2, 2 mM phosphoenolpyruvate, 0.7 mM NADH, 3 U/ml Pyruvate kinase, 2.5 U/ml Lactate dehydrogenase, 0.1% (v/v) dimethylsulfoxide
PH: 8
Temperature: 25
uniprot:Q5GA17 [5-Bromo-2-(5-{[1-(3,4-dichlorophenyl)-3,5-dioxo-4-pyrazolidinylidene]methyl}-2-furyl)benzoic acid]

Shikimate + ATP -> Shikimate_3_phosphate + ADP		 (( (Vmax * S) ) / (S + (Km * (1 + (I / Ki))))) buffer: 100 mM Tris-HCl, 50 mM KCl, 5 mM MgCl2, 2 mM phosphoenolpyruvate, 0.7 mM NADH, 3 U/ml Pyruvate kinase, 2.5 U/ml Lactate dehydrogenase, 0.1% (v/v) dimethylsulfoxide
PH: 8
Temperature: 25

Pathways

pathway id name
BioCyc:META_PWY-6628 superpathway of L-phenylalanine biosynthesis
BioCyc:META_PWY-6165 chorismate biosynthesis II (archaea)
BioCyc:META_ALL-CHORISMATE-PWY superpathway of chorismate metabolism
BioCyc:ECO_COMPLETE-ARO-PWY superpathway of aromatic amino acid biosynthesis
BioCyc:YEAST_PWY-6164 3-dehydroquinate biosynthesis
BioCyc:YEAST_ARO-YEAST-PWY chorismate biosynthesis
BioCyc:VCHO_ARO-PWY chorismate biosynthesis I
BioCyc:MTBH37RV_PWY-6629 superpathway of L-tryptophan biosynthesis
BioCyc:MTBH37RV_ARO-PWY chorismate biosynthesis I
BioCyc:ECOO157_PWY-6630 superpathway of tyrosine biosynthesis
BioCyc:ECOL199310_PWY-6163 chorismate biosynthesis from 3-dehydroquinate
BioCyc:ECOL199310_ARO-PWY chorismate biosynthesis I
BioCyc:ECOL199310_COMPLETE-ARO-PWY superpathway of phenylalanine, tyrosine, and tryptophan biosynthesis
BioCyc:ECOL199310_PWY-6628 superpathway of phenylalanine biosynthesis
BioCyc:BSUB_PWY-6163 chorismate biosynthesis from 3-dehydroquinate
BioCyc:ECOL316407_ALL-CHORISMATE-PWY superpathway of chorismate metabolism
BioCyc:CORYNE_PWY-6629 superpathway of L-tryptophan biosynthesis
BioCyc:META_PWY-6630 superpathway of L-tyrosine biosynthesis
BioCyc:ECO_ARO-PWY chorismate biosynthesis I
BioCyc:VCHO_PWY-6628 superpathway of L-phenylalanine biosynthesis
BioCyc:VCHO_ALL-CHORISMATE-PWY superpathway of chorismate metabolism
BioCyc:VCHO_PWY-6629 superpathway of L-tryptophan biosynthesis
BioCyc:HPY_ARO-PWY chorismate biosynthesis I
BioCyc:ECOO157_COMPLETE-ARO-PWY superpathway of phenylalanine, tyrosine, and tryptophan biosynthesis
BioCyc:ECOO157_PWY-6629 superpathway of tryptophan biosynthesis
BioCyc:BSUB_ARO-PWY chorismate biosynthesis I
BioCyc:PCHR_PWY-6416 quinate degradation II
BioCyc:SYNEL_ARO-PWY chorismate biosynthesis I
BioCyc:ECOL316407_COMPLETE-ARO-PWY superpathway of phenylalanine, tyrosine, and tryptophan biosynthesis
BioCyc:ECOL316407_ARO-PWY chorismate biosynthesis I
BioCyc:CORYNE_PWY-6163 chorismate biosynthesis from 3-dehydroquinate
BioCyc:GCF_000013425_PWY-6163 chorismate biosynthesis from 3-dehydroquinate
BioCyc:META_COMPLETE-ARO-PWY superpathway of aromatic amino acid biosynthesis
BioCyc:META_ARO-PWY chorismate biosynthesis I
BioCyc:META_PWY-6164 3-dehydroquinate biosynthesis I
BioCyc:ECO_PWY-6163 chorismate biosynthesis from 3-dehydroquinate
BioCyc:YEAST_COMPLETE-ARO-YEAST-PWY superpathway of phenylalanine, tyrosine and tryptophan biosynthesis
BioCyc:VCHO_PWY-6163 chorismate biosynthesis from 3-dehydroquinate
BioCyc:MTBCDC1551_PWY-6163 chorismate biosynthesis from 3-dehydroquinate
BioCyc:ECOO157_ARO-PWY chorismate biosynthesis I
BioCyc:ECOO157_PWY-6628 superpathway of phenylalanine biosynthesis
BioCyc:ECOL199310_PWY-6629 superpathway of tryptophan biosynthesis
BioCyc:CAULO_PWY-6163 chorismate biosynthesis from 3-dehydroquinate
BioCyc:BSUB_PWY-6630 superpathway of L-tyrosine biosynthesis
BioCyc:PCHR_ARO-PWY chorismate biosynthesis I
BioCyc:BTHE_ARO-PWY chorismate biosynthesis I
BioCyc:CORYNE_COMPLETE-ARO-PWY superpathway of aromatic amino acid biosynthesis
BioCyc:CORYNE_PWY-6628 superpathway of L-phenylalanine biosynthesis
BioCyc:GCF_000013425_PWY-6630 superpathway of L-tyrosine biosynthesis
BioCyc:META_PWY-6163 chorismate biosynthesis from 3-dehydroquinate
BioCyc:META_PWY-6629 superpathway of L-tryptophan biosynthesis
BioCyc:ECO_ALL-CHORISMATE-PWY superpathway of chorismate metabolism
BioCyc:ARA_PWY-6163 chorismate biosynthesis from 3-dehydroquinate
BioCyc:VCHO_COMPLETE-ARO-PWY superpathway of aromatic amino acid biosynthesis
BioCyc:VCHO_PWY-6630 superpathway of L-tyrosine biosynthesis
BioCyc:MTBH37RV_PWY-6630 superpathway of L-tyrosine biosynthesis
BioCyc:MTBH37RV_PWY-6163 chorismate biosynthesis from 3-dehydroquinate
BioCyc:MTBCDC1551_ARO-PWY chorismate biosynthesis I
BioCyc:HPY_PWY-6163 chorismate biosynthesis from 3-dehydroquinate
BioCyc:ECOO157_PWY-6163 chorismate biosynthesis from 3-dehydroquinate
BioCyc:ECOL199310_PWY-6630 superpathway of tyrosine biosynthesis
BioCyc:ECOL199310_ALL-CHORISMATE-PWY superpathway of chorismate metabolism
BioCyc:CAULO_ARO-PWY chorismate biosynthesis I
BioCyc:BSUB_PWY-6629 superpathway of L-tryptophan biosynthesis
BioCyc:PCHR_PWY-6164 3-dehydroquinate biosynthesis I
BioCyc:PCHR_PWY-6163 chorismate biosynthesis from 3-dehydroquinate
BioCyc:SYNEL_PWY-6163 chorismate biosynthesis from 3-dehydroquinate
BioCyc:SYNEL_PWY-6629 superpathway of L-tryptophan biosynthesis
BioCyc:ECOL316407_PWY-6163 chorismate biosynthesis from 3-dehydroquinate
BioCyc:CAULONA1000_COMPLETE-ARO-PWY superpathway of phenylalanine, tyrosine, and tryptophan biosynthesis
BioCyc:CAULONA1000_ARO-PWY chorismate biosynthesis
BioCyc:BTHE_PWY-6163 chorismate biosynthesis from 3-dehydroquinate
BioCyc:CORYNE_ARO-PWY chorismate biosynthesis I
BioCyc:CORYNE_PWY-6630 superpathway of L-tyrosine biosynthesis
BioCyc:GCF_000013425_PWY-6629 superpathway of L-tryptophan biosynthesis
BioCyc:GCF_000013425_ARO-PWY chorismate biosynthesis I
PathBank:SMP0000836 Chorismate Biosynthesis
PlantCyc:ARA_ARO-PWY chorismate biosynthesis I
PlantCyc:PLANT_ARO-PWY chorismate biosynthesis I
PlantCyc:ARA_PWY-6163 chorismate biosynthesis from 3-dehydroquinate
Reactome:R-MTU-870392 Mycobacterium tuberculosis biological processes
Reactome:R-MTU-964903 Chorismate via Shikimate Pathway
PathBank:SMP0121291 Chorismate Biosynthesis
PathBank:SMP0012009 Shikimate Pathway (Chorismate Biosynthesis)