EC: 5.4.99.2

methylmalonyl-CoA mutase ((R)-methylmalonyl-CoA CoA-carbonylmutase)

enzyme lambda metabolic reaction experiment
uniprot:P22033 [methylmalonyl-CoA mutase(Enzyme) wildtype]

(R)_2_Methyl_3_oxopropanoyl_CoA -> Succinyl_CoA		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 97.2 mM Tris/phosphate, 6.94 µM adenosylcobalamin
PH: 7.4
Temperature: 37
uniprot:P22033 [methylmalonyl-CoA mutase(Enzyme) wildtype]

2_Methylmalonyl_CoA -> Succinyl_CoA		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 97.2 mM Tris/phosphate, 6.94 µM adenosylcobalamin
PH: 7.4
Temperature: 37
uniprot:P22033 [Coenzyme B12]

(R)_2_Methyl_3_oxopropanoyl_CoA -> Succinyl_CoA		 (( (Vmax * S) ) / (Km_app + S)) buffer: 200 mM Tris-sulfate
PH: 7.5
Temperature: 37
uniprot:P22033 [Coenzyme B12]

(R)_2_Methyl_3_oxopropanoyl_CoA -> Succinyl_CoA		 (( (Vmax * S) ) / (Km_app + S)) buffer: 200 mM Tris-sulfate
PH: 7.5
Temperature: 37
uniprot:P22033 [Coenzyme B12]

(R)_2_Methyl_3_oxopropanoyl_CoA -> Succinyl_CoA		 (( (Vmax * S) ) / (Km_app + S)) buffer: 200 mM Tris-sulfate
PH: 7.5
Temperature: 37
uniprot:P22033 [Coenzyme B12]

(R)_2_Methyl_3_oxopropanoyl_CoA -> Succinyl_CoA		 (( (Vmax * A) ) / (Km + A)) buffer: 97.2 mM Tris/phosphate
PH: 7.4
Temperature: 37
uniprot:P22033 [Coenzyme B12]

(R)_2_Methyl_3_oxopropanoyl_CoA -> Succinyl_CoA		 (( (Vmax * A) ) / (Km + A)) buffer: 97.2 mM Tris/phosphate
PH: 7.4
Temperature: 37
uniprot:P22033 [Coenzyme B12]

(R)_2_Methyl_3_oxopropanoyl_CoA -> Succinyl_CoA		 (( (Vmax * A) ) / (Km + A)) buffer: 97.2 mM Tris/phosphate
PH: 7.4
Temperature: 37
uniprot:P22033 [Coenzyme B12]

(R)_2_Methyl_3_oxopropanoyl_CoA -> Succinyl_CoA		 (( (Vmax * A) ) / (Km + A)) buffer: 97.2 mM Tris/phosphate
PH: 7.4
Temperature: 37
uniprot:P22033 [Coenzyme B12]

(R)_2_Methyl_3_oxopropanoyl_CoA -> Succinyl_CoA		 (( (Vmax * A) ) / (Km + A)) buffer: 97.2 mM Tris/phosphate
PH: 7.4
Temperature: 37
uniprot:P22033 [Coenzyme B12]

(R)_2_Methyl_3_oxopropanoyl_CoA -> Succinyl_CoA		 (( (Vmax * A) ) / (Km + A)) buffer: 97.2 mM Tris/phosphate
PH: 7.4
Temperature: 37
uniprot:P22033 [Coenzyme B12]

(R)_2_Methyl_3_oxopropanoyl_CoA -> Succinyl_CoA		 (( (Vmax * A) ) / (Km + A)) buffer: 97.2 mM Tris/phosphate
PH: 7.4
Temperature: 37
uniprot:P22033 [Ethylmalonyl-CoA]

2_Methylmalonyl_CoA -> Succinyl_CoA		 (( (Vmax * S) ) / (( (Km * (1 + (I / Ki1))) ) + (S * (1 + (I / Ki2))))) buffer: 50 mM Tris-phosphate, 10 mM MgCl2, 1 mM GDP, 0.33 U / ml Thiokinase, 50 µM DTNB, 10 µM Adenosylcobamin
PH: 7.5
Temperature: 30
uniprot:P22033 [methylmalonyl-CoA mutase(Enzyme) wildtype]

2_Methylmalonyl_CoA -> Succinyl_CoA		 (( (Vmax * S) ) / (Km_app + S)) buffer: 50 mM Tris-phosphate, 10 mM MgCl2, 1 mM GDP, 0.33 U / ml Thiokinase, 50 µM DTNB, 10 µM Adenosylcobalamin
PH: 7.5
Temperature: 37
uniprot:P22033 [methylmalonyl-CoA mutase(Enzyme) wildtype]

2_Methylmalonyl_CoA -> Succinyl_CoA		 (( (Vmax * S) ) / (Km_app + S)) buffer: 50 mM Tris-phosphate, 10 mM MgCl2, 1 mM GDP, 0.33 U / ml Thiokinase, 50 µM DTNB, 10 µM Adenosylcobalamin
PH: 7.5
Temperature: 30
uniprot:P22033 [Coenzyme B12]

2_Methylmalonyl_CoA -> Succinyl_CoA		 (( (Vmax * S) ) / (( (Km * (1 + (I / Ki1))) ) + (S * (1 + (I / Ki2))))) buffer: 50 mM Tris-phosphate, 10 mM MgCl2, 1 mM GDP, 0.33 U / ml Thiokinase, 50 µM DTNB, 10 µM Adenosylcobalamin
PH: 7.5
Temperature: 30
uniprot:P22033 [Cyclopropylcarbonyl-CoA carboxylate]

2_Methylmalonyl_CoA -> Succinyl_CoA		 (( (Vmax * S) ) / (( (Km * (1 + (I / Ki1))) ) + (S * (1 + (I / Ki2))))) buffer: 50 mM Tris-phosphate, 10 mM MgCl2, 1 mM GDP, 0.33 U / ml Thiokinase, 50 µM DTNB, 10 µM Adenosylcobalamin
PH: 7.5
Temperature: 30

Pathways

pathway id name
BioCyc:HUMAN_PWY-5328 superpathway of methionine degradation
BioCyc:META_P108-PWY pyruvate fermentation to propanoate I
BioCyc:META_PWY0-43 conversion of succinate to propanoate
BioCyc:META_PWY-5328 superpathway of L-methionine salvage and degradation
BioCyc:MTBCDC1551_PROPIONMET-PWY propionyl CoA degradation
BioCyc:META_PWY-5130 2-oxobutanoate degradation I
BioCyc:ECO_PROPIONMET-PWY propanoyl CoA degradation I
BioCyc:MOUSE_PWY-5328 superpathway of methionine degradation
BioCyc:MOUSE_PWY3DJ-0 isoleucine degradation
BioCyc:HUMAN_PROPIONMET-PWY propionyl-CoA degradation
BioCyc:MTBH37RV_PROPIONMET-PWY propanoyl CoA degradation I
BioCyc:MOUSE_PROPIONMET-PWY methylmalonyl pathway
BioCyc:HUMAN_PWY-5130 2-oxobutanoate degradation
BioCyc:META_PWY-7854 crotonyl-CoA/ethylmalonyl-CoA/hydroxybutyryl-CoA cycle (engineered)
BioCyc:META_PROPIONMET-PWY propanoyl CoA degradation I
BioCyc:ECO_PWY0-43 conversion of succinate to propanoate
BioCyc:MOUSE_PWY3DJ-86 valine degradation
BioCyc:ECOL316407_PWY0-43 conversion of succinate to propionate
PathBank:SMP0000957 Propanoate Metabolism
PathBank:SMP0002069 Propanoyl-CoA Degradation
PathBank:SMP0002070 Conversion of Succinate to Propanoate
Reactome:R-CEL-9759218 Cobalamin (Cbl) metabolism
Reactome:R-HSA-9759218 Cobalamin (Cbl) metabolism
Reactome:R-MMU-9759218 Cobalamin (Cbl) metabolism
Reactome:R-HSA-196849 Metabolism of water-soluble vitamins and cofactors
Reactome:R-HSA-1643685 Disease
Reactome:R-HSA-3296469 Defects in cobalamin (B12) metabolism
Reactome:R-MMU-1430728 Metabolism
Reactome:R-MMU-196849 Metabolism of water-soluble vitamins and cofactors
Reactome:R-CEL-196849 Metabolism of water-soluble vitamins and cofactors
Reactome:R-HSA-196854 Metabolism of vitamins and cofactors
Reactome:R-HSA-196741 Cobalamin (Cbl, vitamin B12) transport and metabolism
Reactome:R-HSA-3296482 Defects in vitamin and cofactor metabolism
Reactome:R-HSA-3359475 Defective MMAA causes MMA, cblA type
Reactome:R-MMU-196854 Metabolism of vitamins and cofactors
Reactome:R-MMU-196741 Cobalamin (Cbl, vitamin B12) transport and metabolism
Reactome:R-HSA-5668914 Diseases of metabolism
Reactome:R-CEL-1430728 Metabolism
Reactome:R-CEL-196854 Metabolism of vitamins and cofactors
Reactome:R-CEL-196741 Cobalamin (Cbl, vitamin B12) transport and metabolism
Reactome:R-HSA-1430728 Metabolism
WikiPathways:WP4686 Leucine, isoleucine and valine metabolism
WikiPathways:WP4271 Vitamin B12 disorders
PathBank:SMP0087234 Valine, Leucine, and Isoleucine Degradation
PathBank:SMP0087262 Threonine and 2-Oxobutanoate Degradation
PathBank:SMP0087248 Propanoate Metabolism
PathBank:SMP0087480 Propanoate Metabolism
PathBank:SMP0087516 Threonine and 2-Oxobutanoate Degradation
PathBank:SMP0000452 Threonine and 2-Oxobutanoate Degradation