EC: 4.1.3.27

anthranilate synthase (chorismate pyruvate-lyase (amino-accepting; anthranilate-forming))

enzyme lambda metabolic reaction experiment
- [anthranilate synthase(Enzyme) wildtype]

L_Glutamine + Chorismate -> L_Glutamate + Pyruvate + Anthranilate		 (( (Vmax * B) ) / (Km + B)) buffer: 35 mM Tris-HCl, 2 mM MgCl2, 50 µM EDTA, 50 µM DTT
PH: 8
Temperature: 30
- [anthranilate synthase(Enzyme) mutant trp5-1 (D341N)]

Chorismate + L_Glutamine -> Anthranilate + Pyruvate + L_Glutamate		 (( (Vmax * B) ) / (Km + B)) buffer: 35 mM Tris-HCl, 2 mM MgCl2, 50 µM EDTA, 50 µM DTT
PH: 8
Temperature: 30
- [Mg2+]

Chorismate + L_Glutamine -> Anthranilate + Pyruvate + L_Glutamate		 (( (Vmax * B) ) / (Km + B)) buffer: 8.25 mM Tris, 0.33 mM EDTA
PH: 7.5
Temperature: 37
- [Mg2+]

L_Glutamine + Chorismate -> Anthranilate + Pyruvate + L_Glutamate		 (( (Vmax * A) ) / (Km + A)) buffer: 8.25 mM Tris, 0.33 mM EDTA
PH: 7.5
Temperature: 37
uniprot:Q9AW95 [Mg2+]

L_Glutamine + Chorismate -> Pyruvate + L_Glutamate + Anthranilate		 (( (Vmax * S) ) / (Km + S)) buffer: 0.1 M Tris/HCl
PH: 7.5
Temperature: 30
uniprot:Q9AW95 [Mg2+]

L_Glutamine + Chorismate -> Anthranilate + L_Glutamate + Pyruvate		 (( (Vmax * S) ) / (Km + S)) buffer: 0.1 M Tris/HCl
PH: 7.5
Temperature: 30
uniprot:Q9AW95 [Mg2+]

L_Glutamine + Chorismate -> L_Glutamate + Anthranilate + Pyruvate		 (( (Vmax * S) ) / (Km + S)) buffer: 0.1 M Tris/HCl
PH: 7.5
Temperature: 30
uniprot:Q9AW95 [L-Tryptophan]

L_Glutamine + Chorismate -> L_Glutamate + Pyruvate + Anthranilate		 (( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) buffer: 0.1 M Tris/HCl
PH: 7.5
Temperature: 30
uniprot:Q9AW95 [Mg2+]

NH3 + Chorismate -> Anthranilate + Pyruvate + H2O		 (( (Vmax * S) ) / (Km + S)) buffer: 20 mM triethanolamine, 10% glycerol, 1 mM EDTA
PH: 8.5
Temperature: 30
uniprot:P00899 [Mg2+]

L_Glutamine + Chorismate -> Anthranilate + L_Glutamate + Pyruvate		 (( (Vmax * A) ) / (Km + A)) buffer: 20 mM Tris-HCl, 0.1 mM EDTA
PH: 8.2
Temperature: 30
uniprot:P00899 [Mg2+]

Chorismate + L_Glutamine -> Pyruvate + Anthranilate + L_Glutamate		 (( (Vmax * B) ) / (Km + B)) buffer: 20 mM Tris-HCl, 0.1 mM EDTA
PH: 8.2
Temperature: 30
uniprot:P00899 [Mg2+]

L_Glutamine + Chorismate -> Pyruvate + Anthranilate + L_Glutamate		 (( (Vmax * C) ) / (Km + C)) buffer: 20 mM Tris-HCl, 0.1 mM EDTA
PH: 8.2
Temperature: 30
uniprot:P00899 [Mg2+]

Chorismate + NH3 -> Pyruvate + H2O + Anthranilate		 (( (Vmax * B) ) / (Km + B)) buffer: 50 mM Tris-HCl
PH: 8.55
Temperature: 36
uniprot:P00899 [Mg2+]

Chorismate + NH3 -> Pyruvate + Anthranilate + H2O		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Tris-HCl
PH: 8.55
Temperature: 36

Pathways

pathway id name
BioCyc:META_ALL-CHORISMATE-PWY superpathway of chorismate metabolism
BioCyc:ECO_COMPLETE-ARO-PWY superpathway of aromatic amino acid biosynthesis
BioCyc:YEAST_TRPSYN-PWY tryptophan biosynthesis
BioCyc:MTBH37RV_PWY-6629 superpathway of L-tryptophan biosynthesis
BioCyc:MTBCDC1551_PWY-6543 4-aminobenzoate biosynthesis
BioCyc:MTBCDC1551_TRPSYN-PWY L-tryptophan biosynthesis
BioCyc:BSUB_TRPSYN-PWY L-tryptophan biosynthesis
BioCyc:ECOL316407_TRPSYN-PWY tryptophan biosynthesis
BioCyc:ECOL316407_ALL-CHORISMATE-PWY superpathway of chorismate metabolism
BioCyc:CORYNE_PWY-6629 superpathway of L-tryptophan biosynthesis
BioCyc:VCHO_ALL-CHORISMATE-PWY superpathway of chorismate metabolism
BioCyc:VCHO_PWY-6629 superpathway of L-tryptophan biosynthesis
BioCyc:HPY_TRPSYN-PWY L-tryptophan biosynthesis
BioCyc:BSUB_PWY-6612 superpathway of tetrahydrofolate biosynthesis
BioCyc:ECOL316407_COMPLETE-ARO-PWY superpathway of phenylalanine, tyrosine, and tryptophan biosynthesis
BioCyc:CORYNE_TRPSYN-PWY L-tryptophan biosynthesis
BioCyc:META_PWY-6661 4-hydroxy-2(1H)-quinolone biosynthesis
BioCyc:META_PWY-6662 superpathway of quinolone and alkylquinolone biosynthesis
BioCyc:META_PWY-5770 phenazine-1-carboxylate biosynthesis
BioCyc:META_COMPLETE-ARO-PWY superpathway of aromatic amino acid biosynthesis
BioCyc:YEAST_COMPLETE-ARO-YEAST-PWY superpathway of phenylalanine, tyrosine and tryptophan biosynthesis
BioCyc:VCHO_TRPSYN-PWY L-tryptophan biosynthesis
BioCyc:MTBH37RV_TRPSYN-PWY L-tryptophan biosynthesis
BioCyc:CORYNE_COMPLETE-ARO-PWY superpathway of aromatic amino acid biosynthesis
BioCyc:META_TRPSYN-PWY L-tryptophan biosynthesis
BioCyc:META_PWY-6629 superpathway of L-tryptophan biosynthesis
BioCyc:ECO_ALL-CHORISMATE-PWY superpathway of chorismate metabolism
BioCyc:ECO_TRPSYN-PWY L-tryptophan biosynthesis
BioCyc:ARA_COMPLETE-ARO-ARA-PWY superpathway of phenylalanine, tyrosine and tryptophan biosynthesis
BioCyc:ARA_TRPSYN-PWY L-tryptophan biosynthesis
BioCyc:VCHO_COMPLETE-ARO-PWY superpathway of aromatic amino acid biosynthesis
BioCyc:MTBCDC1551_PWY-6612 superpathway of tetrahydrofolate biosynthesis
BioCyc:MTBCDC1551_FOLSYN-PWY superpathway of tetrahydrofolate biosynthesis and salvage
BioCyc:BSUB_PWY-6629 superpathway of L-tryptophan biosynthesis
BioCyc:BSUB_PWY-6543 4-aminobenzoate biosynthesis
BioCyc:BSUB_FOLSYN-PWY superpathway of tetrahydrofolate biosynthesis and salvage
PathBank:SMP0000835 Tryptophan Metabolism
PathBank:SMP0000981 Trp Operon
PathBank:SMP0001930 Tryptophan Metabolism II
Plant Reactome:R-OSA-9030769 Growth and developmental processes
Plant Reactome:R-OSA-9623902 Seed development
Plant Reactome:R-OSA-9031669 Reproductive structure development
Plant Reactome:R-OSA-9631623 Regulation of embryo development
PlantCyc:ARA_COMPLETE-ARO-ARA-PWY superpathway of phenylalanine, tyrosine and tryptophan biosynthesis
PlantCyc:ARA_TRPSYN-PWY L-tryptophan biosynthesis
PlantCyc:PLANT_TRPSYN-PWY L-tryptophan biosynthesis
PathBank:SMP0002353 Tryptophan Metabolism
PathBank:SMP0002445 Tryptophan Metabolism