EC: 3.2.2.27

uracil-DNA glycosylase (uracil-DNA deoxyribohydrolase (uracil-releasing))

enzyme lambda metabolic reaction experiment
uniprot:P13051 [uracil-DNA glycosylase(Enzyme) wildtype]

H2O + DNA -> Uracil + Apyrimidinic_site_in_DNA		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 1 mM EDTA, 50 mM NaCl, 0.1 mg/ml bovine serum albumin, 2 mM MgCl2
PH: 7.6
Temperature: 25
uniprot:P13051 [uracil-DNA glycosylase(Enzyme) wildtype]

DNA + H2O -> Uracil + Apyrimidinic_site_in_DNA		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 1 mM EDTA, 50 mM NaCl, 0.1 mg/ml bovine serum albumin, 2 mM MgCl2
PH: 7.6
Temperature: 25
uniprot:P13051 [uracil-DNA glycosylase(Enzyme) wildtype]

H2O + DNA -> Apyrimidinic_site_in_DNA + Uracil		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 1 mM EDTA, 50 mM NaCl, 0.1 mg/ml bovine serum albumin, 2 mM MgCl2
PH: 7.6
Temperature: 25
uniprot:A0A291IS31 [uracil-DNA glycosylase(Enzyme) wildtype]

DNA + H2O -> Apyrimidinic_site_in_DNA + Uracil		 (( (Vmax * S) ) / (S + Km)) buffer: 50 mM Tris hydrochloride, 100 µg/ml bovine serum albumin, 20 mM dithiothreitol, 10 mM EDTA
PH: 7.5
Temperature: 37

Pathways

pathway id name
BioCyc:MOUSE_PWY-6556 pyrimidine ribonucleosides degradation II
BioCyc:MOUSE_P1-PWY purine and pyrimidine metabolism
Reactome:R-HSA-9609690 HCMV Early Events
Reactome:R-HSA-9609646 HCMV Infection
Reactome:R-HSA-9824446 Viral Infection Pathways
Reactome:R-DDI-73894 DNA Repair
Reactome:R-DDI-110357 Displacement of DNA glycosylase by APEX1
Reactome:R-HSA-1643685 Disease
Reactome:R-MMU-73933 Resolution of Abasic Sites (AP sites)
Reactome:R-SCE-73929 Base-Excision Repair, AP Site Formation
Reactome:R-SCE-110329 Cleavage of the damaged pyrimidine
Reactome:R-SPO-73929 Base-Excision Repair, AP Site Formation
Reactome:R-SPO-110329 Cleavage of the damaged pyrimidine
Reactome:R-DDI-73933 Resolution of Abasic Sites (AP sites)
Reactome:R-MMU-73929 Base-Excision Repair, AP Site Formation
Reactome:R-MMU-110329 Cleavage of the damaged pyrimidine
Reactome:R-SCE-73884 Base Excision Repair
Reactome:R-SCE-73928 Depyrimidination
Reactome:R-SPO-73884 Base Excision Repair
Reactome:R-SPO-73928 Depyrimidination
Reactome:R-DDI-73884 Base Excision Repair
Reactome:R-DDI-73928 Depyrimidination
Reactome:R-MMU-73894 DNA Repair
Reactome:R-MMU-110357 Displacement of DNA glycosylase by APEX1
Reactome:R-DDI-73929 Base-Excision Repair, AP Site Formation
Reactome:R-DDI-110329 Cleavage of the damaged pyrimidine
Reactome:R-HSA-5663205 Infectious disease
Reactome:R-MMU-73884 Base Excision Repair
Reactome:R-MMU-73928 Depyrimidination
Reactome:R-SCE-73894 DNA Repair
Reactome:R-SPO-73894 DNA Repair