EC: 2.7.1.134

inositol-tetrakisphosphate 1-kinase (ATP:1D-myo-inositol-3,4,5,6-tetrakisphosphate 1-phosphotransferase)

enzyme lambda metabolic reaction experiment
uniprot:Q13572 [inositol-tetrakisphosphate 1-kinase(Enzyme) wildtype ITPK1]

ADP + 1D_myo_Inositol_1,3,4,5,6_pentakisphosphate -> ATP + 1D_myo_Inositol_3,4,5,6_tetrakisphosphate		 (( (Vmax * S) ) / (Km + S)) buffer: 20 mM Tris-HCl, 1 mM 2-mercaptoethanol, 1 mM MgCl2, 10% glycerol
PH: 7.6
Temperature: 37
uniprot:Q13752 [inositol-tetrakisphosphate 1-kinase(Enzyme) wildtype ITPK1]

ATP + 1D_myo_Inositol_3,4,5,6_tetrakisphosphate -> ADP + 1D_myo_Inositol_1,3,4,5,6_pentakisphosphate		 (( (Vmax * S) ) / (Km + S)) buffer: 20 mM Tris-HCl, 1 mM 2-mercaptoethanol, 1 mM MgCl2, 10% glycerol
PH: 7.6
Temperature: 37
- [D-myo-Inositol 1,4,5-trisphosphate]

ATP + 1D_myo_Inositol_3,4,5,6_tetrakisphosphate -> 1D_myo_Inositol_1,3,4,5,6_pentakisphosphate + ADP		 (( (Vmax * S) ) / (( (Km * (1 + (I / Ki))) ) + S)) buffer: 20 mM HEPES, 6 mM MgSO4, 0.4 mg/ml saponin, 100 mM KCl, 0.3 mg/ml BSA, 10 mM phosphocreatine, 25 Sigma U/ml phosphocreatine kinase
PH: 7.2
Temperature: 37
- [1D-myo-Inositol 1,3,4,6-tetrakisphosphate]

ATP + 1D_myo_Inositol_3,4,5,6_tetrakisphosphate -> ADP + 1D_myo_Inositol_1,3,4,5,6_pentakisphosphate		 (( (Vmax * S) ) / (( (Km * (1 + (I / Ki))) ) + S)) buffer: 20 mM HEPES, 6 mM MgSO4, 0.4 mg/ml saponin, 100 mM KCl, 0.3 mg/ml BSA, 10 mM phosphocreatine, 25 Sigma U/ml phosphocreatine kinase
PH: 7.2
Temperature: 37
- [1D-myo-Inositol 1,3,4,5-tetrakisphosphate]

1D_myo_Inositol_3,4,5,6_tetrakisphosphate + ATP -> 1D_myo_Inositol_1,3,4,5,6_pentakisphosphate + ADP		 (( (Vmax * S) ) / (( (Km * (1 + (I / Ki))) ) + S)) buffer: 20 mM HEPES, 6 mM MgSO4, 0.4 mg/ml saponin, 100 mM KCl, 0.3 mg/ml BSA, 10 mM phosphocreatine, 25 Sigma U/ml phosphocreatine kinase
PH: 7.2
Temperature: 37
- [1D-myo-Inositol 1,3,4-trisphosphate]

1D_myo_Inositol_3,4,5,6_tetrakisphosphate + ATP -> ADP + 1D_myo_Inositol_1,3,4,5,6_pentakisphosphate		 (( (Vmax * S) ) / (( (Km * (1 + (I / Ki))) ) + S)) buffer: 20 mM HEPES, 6 mM MgSO4, 0.4 mg/ml saponin, 100 mM KCl, 0.3 mg/ml BSA, 10 mM phosphocreatine, 25 Sigma U/ml phosphocreatine kinase
PH: 7.2
Temperature: 37
- [1D-myo-Inositol 1,3,4,5,6-pentakisphosphate]

ATP + 1D_myo_Inositol_3,4,5,6_tetrakisphosphate -> ADP + 1D_myo_Inositol_1,3,4,5,6_pentakisphosphate		 (( (Vmax * S) ) / (( (Km * (1 + (I / Ki))) ) + S)) buffer: 20 mM HEPES, 6 mM MgSO4, 0.4 mg/ml saponin, 100 mM KCl, 0.3 mg/ml BSA, 10 mM phosphocreatine, 25 Sigma U/ml phosphocreatine kinase
PH: 7.2
Temperature: 37
- [inositol-tetrakisphosphate 1-kinase(Enzyme) wildtype]

1D_myo_Inositol_3,4,5,6_tetrakisphosphate + ATP -> 1D_myo_Inositol_1,3,4,5,6_pentakisphosphate + ADP		 (( (Vmax * S) ) / (Km + S)) buffer: 20 mM HEPES, 6 mM MgSO4, 0.4 mg/ml saponin, 100 mM KCl, 0.3 mg/ml BSA, 10 mM phosphocreatine, 25 Sigma U/ml phosphocreatine kinase
PH: 7.2
Temperature: 37
- [inositol-tetrakisphosphate 1-kinase(Enzyme) wildtype]

1D_myo_Inositol_3,4,5,6_tetrakisphosphate + ATP -> ADP + 1D_myo_Inositol_1,3,4,5,6_pentakisphosphate		 (( (Vmax * S) ) / (Km + S)) buffer: 20 mM HEPES, 6 mM MgSO4, 0.4 mg/ml saponin, 100 mM KCl, 0.3 mg/ml BSA, 10 mM phosphocreatine, 25 Sigma U/ml phosphocreatine kinase
PH: 7.2
Temperature: 37
uniprot:Q13572 [inositol-tetrakisphosphate 1-kinase(Enzyme) wildtype isoenzyme ITPK1]

ATP + 1D_myo_Inositol_3,4,5,6_tetrakisphosphate -> 1D_myo_Inositol_1,3,4,5,6_pentakisphosphate + ADP		 (( (Vmax * S1) ) / (Km1 + S1)) buffer: 20 mM HEPES, 6 mM MgSO4, 100 mM KCl, 0.3 mg/ml bovine serum albumin, 0.4 mg/ml saponin, 10 mM phosphocreatine, 25 U/ml phosphocreatine kinase
PH: 7.2
Temperature: 37

Pathways

pathway id name
BioCyc:HUMAN_PWY-6366 D-myo-inositol (1,4,5,6)-tetrakisphosphate biosynthesis
BioCyc:ARA_PWY-4562 lipid-dependent phytate biosynthesis II (via Ins(1,3,4)P3)
BioCyc:MOUSE_PWY-6365 D-myo-inositol (3,4,5,6)-tetrakisphosphate biosynthesis
BioCyc:HUMAN_PWY-6365 D-myo-inositol (3,4,5,6)-tetrakisphosphate biosynthesis
BioCyc:META_PWY-6554 1D-myo-inositol hexakisphosphate biosynthesis V (from Ins(1,3,4)P3)
BioCyc:META_PWY-6362 1D-myo-inositol hexakisphosphate biosynthesis II (mammalian)
BioCyc:META_PWY-6366 D-myo-inositol (1,4,5,6)-tetrakisphosphate biosynthesis
BioCyc:ARA_PWY-6554 1D-myo-inositol hexakisphosphate biosynthesis V (from Ins(1,3,4)P3)
BioCyc:ARA_PWY-4765 superpathway of lipid-dependent phytate biosynthesis
BioCyc:HUMAN_PWY-6554 1D-myo-inositol hexakisphosphate biosynthesis V (from Ins(1,3,4)P3)
BioCyc:HUMAN_PWY-6362 1D-myo-inositol hexakisphosphate biosynthesis II (mammalian)
BioCyc:HUMAN_PWY-6371 superpathway of inositol phosphate compounds
BioCyc:META_PWY-6555 superpathway of 1D-myo-inositol hexakisphosphate biosynthesis (plants)
BioCyc:META_PWY-6365 D-myo-inositol (3,4,5,6)-tetrakisphosphate biosynthesis
BioCyc:ARA_PWY-4661 1D-myo-inositol hexakisphosphate biosynthesis III (Spirodela polyrrhiza)
BioCyc:MOUSE_PWY-6362 1D-myo-inositol hexakisphosphate biosynthesis II (mammalian)
PlantCyc:ARA_PWY-4661 1D-myo-inositol hexakisphosphate biosynthesis III (Spirodela polyrrhiza)
PlantCyc:PLANT_PWY-4765 superpathway of lipid-dependent phytate biosynthesis
PlantCyc:PLANT_PWY-6555 superpathway of 1D-myo-inositol hexakisphosphate biosynthesis (plants)
PlantCyc:PLANT_PWY-6554 1D-myo-inositol hexakisphosphate biosynthesis V (from Ins(1,3,4)P3)
PlantCyc:ARA_PWY-4562 lipid-dependent phytate biosynthesis II (via Ins(1,3,4)P3)
PlantCyc:ARA_PWY-4765 superpathway of lipid-dependent phytate biosynthesis
PlantCyc:PLANT_PWY-4661 1D-myo-inositol hexakisphosphate biosynthesis III (Spirodela polyrrhiza)
PlantCyc:PLANT_PWY-4562 lipid-dependent phytate biosynthesis II (via Ins(1,3,4)P3)
PlantCyc:ARA_PWY-6554 1D-myo-inositol hexakisphosphate biosynthesis V (from Ins(1,3,4)P3)
Reactome:R-BTA-109582 Hemostasis
Reactome:R-BTA-1430728 Metabolism
Reactome:R-GGA-1855204 Synthesis of IP3 and IP4 in the cytosol
Reactome:R-GGA-983231 Factors involved in megakaryocyte development and platelet production
Reactome:R-MMU-1430728 Metabolism
Reactome:R-BTA-1855204 Synthesis of IP3 and IP4 in the cytosol
Reactome:R-GGA-1483249 Inositol phosphate metabolism
Reactome:R-HSA-1855204 Synthesis of IP3 and IP4 in the cytosol
Reactome:R-HSA-1855167 Synthesis of pyrophosphates in the cytosol
Reactome:R-MMU-1855204 Synthesis of IP3 and IP4 in the cytosol
Reactome:R-MMU-983231 Factors involved in megakaryocyte development and platelet production
Reactome:R-BTA-983231 Factors involved in megakaryocyte development and platelet production
Reactome:R-BTA-1855167 Synthesis of pyrophosphates in the cytosol
Reactome:R-GGA-1430728 Metabolism
Reactome:R-GGA-109582 Hemostasis
Reactome:R-HSA-109582 Hemostasis
Reactome:R-MMU-1855167 Synthesis of pyrophosphates in the cytosol
Reactome:R-BTA-1483249 Inositol phosphate metabolism
Reactome:R-GGA-1855167 Synthesis of pyrophosphates in the cytosol
Reactome:R-HSA-983231 Factors involved in megakaryocyte development and platelet production
Reactome:R-HSA-1430728 Metabolism
Reactome:R-HSA-1483249 Inositol phosphate metabolism
Reactome:R-MMU-1483249 Inositol phosphate metabolism
Reactome:R-MMU-109582 Hemostasis
WikiPathways:WP5381 Smith-Magenis and Potocki-Lupski syndrome copy number variation
PathBank:SMP0087242 Inositol Metabolism
PathBank:SMP0000011 Inositol Metabolism
PathBank:SMP0000462 Inositol Phosphate Metabolism
PathBank:SMP0087256 Inositol Phosphate Metabolism
PathBank:SMP0012028 Phytate Biosynthesis