EC: 2.4.1.22

lactose synthase (UDP-galactose:D-glucose 4-beta-D-galactosyltransferase)

enzyme lambda metabolic reaction experiment
uniprot:P80225 [alpha-Lactalbumin]

UDP_D_galactose + D_Glucose -> UDP + alpha_Lactose		 (( (Vmax * A) ) / (Km + A)) buffer: 0.1 M MES, 0.5 % (v/v) Triton X-100
PH: 7.5
Temperature: 37
uniprot:P80225 [alpha-Lactalbumin]

D_Glucose + UDP_D_galactose -> alpha_Lactose + UDP		 (( (Vmax * M1) ) / (Ka + M1)) buffer: 0.1 M MES, 0.5 % (v/v) Triton X-100
PH: 7.5
Temperature: 37
uniprot:P80225 [alpha-Lactalbumin]

UDP_D_galactose + D_Glucose -> UDP + alpha_Lactose		 (( (Vmax * M1) ) / (Ka + M1)) buffer: 0.1 M MES, 0.5 % (v/v) Triton X-100
PH: 7.5
Temperature: 37
uniprot:P80225 [alpha-Lactalbumin]

D_Glucose + UDP_D_galactose -> alpha_Lactose + UDP		 (( (Vmax * A) ) / (Km + A)) buffer: 0.1 M MES, 0.5 % (v/v) Triton X-100
PH: 7.5
Temperature: 37
uniprot:P80225 [alpha-Lactalbumin]

D_Glucose + UDP_D_galactose -> alpha_Lactose + UDP		 (( (Vmax * A) ) / (Km + A)) buffer: 0.1 M MES, 0.5 % (v/v) Triton X-100
PH: 7.5
Temperature: 37
uniprot:P80225 [alpha-Lactalbumin]

UDP_D_galactose + D_Glucose -> UDP + alpha_Lactose		 (( (Vmax * A) ) / (Km + A)) buffer: 0.1 M MES, 0.5 % (v/v) Triton X-100
PH: 7.5
Temperature: 37
uniprot:P08037 [alpha-Lactalbumin]

UDP_D_galactose + D_Glucose -> UDP + alpha_Lactose		 (( (Vmax * M1) ) / (Ka + M1)) buffer: 0.1 M MES, 0.5 % (v/v) Triton X-100
PH: 7.5
Temperature: 37
uniprot:P08037 [alpha-Lactalbumin]

D_Glucose + UDP_D_galactose -> UDP + alpha_Lactose		 (( (Vmax * A) ) / (Km + A)) buffer: 0.1 M MES, 0.5 % (v/v) Triton X-100
PH: 7.5
Temperature: 37
uniprot:P08037 [alpha-Lactalbumin]

D_Glucose + UDP_D_galactose -> UDP + alpha_Lactose		 (( (Vmax * A) ) / (Km + A)) buffer: 0.1 M MES, 0.5 % (v/v) Triton X-100
PH: 7.5
Temperature: 37

Pathways

pathway id name
BioCyc:HUMAN_PWY-7835 super pathway of glycosphingolipids biosynthesis
BioCyc:HUMAN_PWY-7434 terminal O-glycans residues modification (via type 2 precursor disaccharide)
BioCyc:HUMAN_PWY-7831 biosynthesis of ABH and Lewis epitopes from type 2 precursor disaccharide
BioCyc:META_PWY-7831 ABH and Lewis epitopes biosynthesis from type 2 precursor disaccharide
BioCyc:META_PWY-7434 terminal O-glycans residues modification (via type 2 precursor disaccharide)
BioCyc:HUMAN_PWY-7841 neolacto-series glycosphingolipids biosynthesis
BioCyc:META_PWY-7835 superpathway of glycosphingolipids biosynthesis
BioCyc:META_PWY-7841 neolacto-series glycosphingolipids biosynthesis
BioCyc:META_PWY-7426 complex N-linked glycan biosynthesis (vertebrates)
BioCyc:HUMAN_PWY-7837 i antigen and I antigen biosynthesis
BioCyc:HUMAN_PWY-7426 complex N-linked glycan biosynthesis (vertebrates)
BioCyc:META_PWY-7837 i antigen and I antigen biosynthesis
Reactome:R-BTA-1430728 Metabolism
Reactome:R-BTA-5653890 Lactose synthesis
Reactome:R-BTA-2534343 Interaction With Cumulus Cells And The Zona Pellucida
Reactome:R-HSA-1474165 Reproduction
Reactome:R-HSA-5653890 Lactose synthesis
Reactome:R-HSA-1630316 Glycosaminoglycan metabolism
Reactome:R-HSA-157118 Signaling by NOTCH
Reactome:R-HSA-1912420 Pre-NOTCH Processing in Golgi
Reactome:R-HSA-392499 Metabolism of proteins
Reactome:R-HSA-597592 Post-translational protein modification
Reactome:R-HSA-975576 N-glycan antennae elongation in the medial/trans-Golgi
Reactome:R-HSA-1643685 Disease
Reactome:R-HSA-3560782 Diseases associated with glycosaminoglycan metabolism
Reactome:R-MMU-1430728 Metabolism
Reactome:R-MMU-5653890 Lactose synthesis
Reactome:R-MMU-1630316 Glycosaminoglycan metabolism
Reactome:R-MMU-975577 N-Glycan antennae elongation
Reactome:R-HSA-2534343 Interaction With Cumulus Cells And The Zona Pellucida
Reactome:R-HSA-71387 Carbohydrate metabolism
Reactome:R-HSA-975577 N-Glycan antennae elongation
Reactome:R-HSA-3781865 Diseases of glycosylation
Reactome:R-HSA-3656244 Defective B4GALT1 causes B4GALT1-CDG (CDG-2d)
Reactome:R-HSA-3781860 Diseases associated with N-glycosylation of proteins
Reactome:R-MMU-1187000 Fertilization
Reactome:R-BTA-71387 Carbohydrate metabolism
Reactome:R-BTA-1474165 Reproduction
Reactome:R-HSA-1187000 Fertilization
Reactome:R-HSA-1638074 Keratan sulfate/keratin metabolism
Reactome:R-HSA-1912422 Pre-NOTCH Expression and Processing
Reactome:R-HSA-446203 Asparagine N-linked glycosylation
Reactome:R-HSA-948021 Transport to the Golgi and subsequent modification
Reactome:R-HSA-5668914 Diseases of metabolism
Reactome:R-HSA-4793953 Defective B4GALT1 causes CDG-2d
Reactome:R-MMU-71387 Carbohydrate metabolism
Reactome:R-MMU-1638074 Keratan sulfate/keratin metabolism
Reactome:R-MMU-392499 Metabolism of proteins
Reactome:R-MMU-597592 Post-translational protein modification
Reactome:R-MMU-975576 N-glycan antennae elongation in the medial/trans-Golgi
Reactome:R-MMU-2534343 Interaction With Cumulus Cells And The Zona Pellucida
Reactome:R-BTA-1187000 Fertilization
Reactome:R-HSA-1430728 Metabolism
Reactome:R-HSA-2022854 Keratan sulfate biosynthesis
Reactome:R-HSA-162582 Signaling Pathways
Reactome:R-MMU-2022854 Keratan sulfate biosynthesis
Reactome:R-MMU-446203 Asparagine N-linked glycosylation
Reactome:R-MMU-948021 Transport to the Golgi and subsequent modification
Reactome:R-MMU-1474165 Reproduction
WikiPathways:WP4142 Metabolism of sphingolipids in ER and Golgi apparatus
WikiPathways:WP4521 Glycosylation and related congenital defects
PathBank:SMP0000444 Lactose Synthesis
PathBank:SMP0000580 GLUT-1 Deficiency Syndrome
PathBank:SMP0000043 Galactose Metabolism
PathBank:SMP0000182 Galactosemia
PathBank:SMP0000579 Congenital Disorder of Glycosylation CDG-IId