EC: 1.1.1.30

3-hydroxybutyrate dehydrogenase ((R)-3-hydroxybutanoate:NAD+ oxidoreductase)

enzyme lambda metabolic reaction experiment
uniprot:Q9BUT1 [3-hydroxybutyrate dehydrogenase(Enzyme) wildtype DHRS6]

NAD+ + (R)_3_Hydroxybutanoate -> NADH + Acetoacetate + H+		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM HEPES
PH: 7.5
Temperature: 25
uniprot:Q9BUT1 [3-hydroxybutyrate dehydrogenase(Enzyme) wildtype DHRS6]

(R)_3_Hydroxybutanoate + NAD+ -> NADH + Acetoacetate + H+		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Tris/Cl
PH: 9
Temperature: 30
uniprot:Q9BUT1 [3-hydroxybutyrate dehydrogenase(Enzyme) wildtype DHRS6]

NAD+ + (R)_3_Hydroxybutanoate -> H+ + NADH + Acetoacetate		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Tris/Cl
PH: 9
Temperature: 25
uniprot:Q9BUT1 [3-hydroxybutyrate dehydrogenase(Enzyme) wildtype DHRS6]

(R)_3_Hydroxybutanoate + NAD+ -> Acetoacetate + NADH + H+		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Tris/Cl
PH: 9
Temperature: 25
- [2-Mercaptoethanol]

(R)_3_Hydroxybutanoate + NAD+ -> Acetoacetate + H+ + NADH		 (( (Vmax * A) ) / (Km + A)) buffer: 37.5 mM Potassium phosphate
PH: 8
Temperature: 30
- [2-Mercaptoethanol]

(R)_3_Hydroxybutanoate + NAD+ -> NADH + H+ + Acetoacetate		 (( (Vmax * A) ) / (Km + A)) buffer: 37.5 mM Potassium phosphate
PH: 8
Temperature: 30
- [2-Mercaptoethanol]

NADH + Acetoacetate + H+ -> NAD+ + (R)_3_Hydroxybutanoate		 (( (Vmax * A) ) / (Km + A)) buffer: 37.5 mM Potassium phosphate
PH: 6.5
Temperature: 30
- [2-Mercaptoethanol]

NADH + Acetoacetate + H+ -> (R)_3_Hydroxybutanoate + NAD+		 (( (Vmax * A) ) / (Km + A)) buffer: 37.5 mM Potassium phosphate
PH: 6.5
Temperature: 30
- [3-hydroxybutyrate dehydrogenase(Enzyme) wildtype]

NAD+ + (R)_3_Hydroxybutanoate -> Acetoacetate + NADH + H+		 (( (Vmax * A) ) / (Km + A)) buffer: 15 mM Tris-HCl, 5 mM EDTA, 0.04 % BSA, 1.3 % Ethanol, 0.3 mM Dithiothreitol, 2.5 µg/ml Antimycin
PH: 8.1
Temperature: 37
- [3-hydroxybutyrate dehydrogenase(Enzyme) wildtype]

Acetoacetate + NADH + H+ -> (R)_3_Hydroxybutanoate + NAD+		 (( (Vmax * A) ) / (Km + A)) buffer: 20 mM Potassium phosphate, 0.04 % BSA, 1.3 % Ethanol, 0.3 mM Dithiothreitol, 2.5 µg/ml Antimycin
PH: 7
Temperature: 37
- [3-hydroxybutyrate dehydrogenase(Enzyme) wildtype]

(R)_3_Hydroxybutanoate + NAD+ -> Acetoacetate + H+ + NADH		 (( (Vmax * A) ) / (Km + A)) buffer: 15 mM Tris-HCl, 5 mM EDTA, 0.04 % BSA, 1.3 % Ethanol, 0.3 mM Dithiothreitol, 2.5 µg/ml Antimycin
PH: 8.1
Temperature: 37
- [3-hydroxybutyrate dehydrogenase(Enzyme) wildtype]

H+ + NADH + Acetoacetate -> (R)_3_Hydroxybutanoate + NAD+		 (( (Vmax * A) ) / (Km + A)) buffer: 20 mM Potassium phosphate, 0.04 % BSA, 1.3 % Ethanol, 0.3 mM Dithiothreitol, 2.5 µg/ml Antimycin
PH: 7
Temperature: 37

Pathways

pathway id name
BioCyc:HUMAN_PWY66-367 ketogenesis
BioCyc:MOUSE_PWY66-368 ketolysis
BioCyc:MOUSE_PWY3FA-1022 ketone oxidation
BioCyc:MOUSE_PWY66-367 ketogenesis
BioCyc:META_PWY66-368 ketolysis
BioCyc:HUMAN_PWY66-368 ketolysis
BioCyc:META_PWY66-367 ketogenesis
BioCyc:MOUSE_PWY3FA-941 ketogenesis
Reactome:R-BTA-1430728 Metabolism
Reactome:R-BTA-556833 Metabolism of lipids
Reactome:R-BTA-77108 Ketone body catabolism
Reactome:R-DRE-74182 Ketone body metabolism
Reactome:R-HSA-77108 Ketone body catabolism
Reactome:R-MMU-1430728 Metabolism
Reactome:R-MMU-556833 Metabolism of lipids
Reactome:R-MMU-77111 Synthesis of Ketone Bodies
Reactome:R-RNO-74182 Ketone body metabolism
Reactome:R-BTA-77111 Synthesis of Ketone Bodies
Reactome:R-HSA-556833 Metabolism of lipids
Reactome:R-HSA-77111 Synthesis of Ketone Bodies
Reactome:R-MMU-74182 Ketone body metabolism
Reactome:R-DRE-1430728 Metabolism
Reactome:R-DRE-556833 Metabolism of lipids
Reactome:R-DRE-77111 Synthesis of Ketone Bodies
Reactome:R-MMU-77108 Ketone body catabolism
Reactome:R-RNO-1430728 Metabolism
Reactome:R-RNO-556833 Metabolism of lipids
Reactome:R-RNO-77111 Synthesis of Ketone Bodies
Reactome:R-BTA-74182 Ketone body metabolism
Reactome:R-HSA-1430728 Metabolism
Reactome:R-HSA-74182 Ketone body metabolism
Reactome:R-RNO-77108 Ketone body catabolism
WikiPathways:WP5175 Disorders in ketone body synthesis
WikiPathways:WP5195 Disorders in ketolysis
WikiPathways:WP4742 Ketogenesis and ketolysis
PathBank:SMP0000071 Ketone Body Metabolism
PathBank:SMP0000569 Succinyl CoA: 3-Ketoacid CoA Transferase Deficiency