EC: 1.1.1.25

shikimate dehydrogenase (NADP+) (shikimate:NADP+ 3-oxidoreductase)

enzyme lambda metabolic reaction experiment
uniprot:Q9SQT8 [shikimate dehydrogenase(Enzyme) mutant D423N, delta1-88, C-terminal his-tag]

Shikimate + NADP+ -> H+ + 3_Dehydroshikimate + NADPH		 (( (( (kcat * E) ) * S1) ) / (Km + S1)) buffer:
PH: 8.8
Temperature: 22
uniprot:Q9SQT8 [shikimate dehydrogenase(Enzyme) mutant K385N/D423N, delta1-88, C-terminal his-tag]

Shikimate + NADP+ -> 3_Dehydroshikimate + H+ + NADPH		 (( (( (kcat * E) ) * S1) ) / (Km + S1)) buffer:
PH: 8.8
Temperature: 22
uniprot:Q9SQT8 [shikimate dehydrogenase(Enzyme) mutant K385A, delta1-88, C-terminal his-tag]

NADP+ + Shikimate -> H+ + NADPH + 3_Dehydroshikimate		 (( (( (kcat * E) ) * S1) ) / (Km + S1)) buffer:
PH: 8.8
Temperature: 22
uniprot:Q9SQT8 [shikimate dehydrogenase(Enzyme) wildtype delta1-88, C-terminal his-tag]

NADP+ + Shikimate -> NADPH + H+ + 3_Dehydroshikimate		 (( (( (kcat * E) ) * S2) ) / (Km + S2)) buffer:
PH: 8.8
Temperature: 22
uniprot:Q9SQT8 [shikimate dehydrogenase(Enzyme) mutant K385N/D423N, delta1-88, C-terminal his-tag]

NADP+ + Shikimate -> H+ + NADPH + 3_Dehydroshikimate		 (( (( (kcat * E) ) * S2) ) / (Km + S2)) buffer:
PH: 8.8
Temperature: 22
uniprot:Q9SQT8 [shikimate dehydrogenase(Enzyme) mutant K385N, delta1-88, C-terminal his-tag]

NADP+ + Shikimate -> H+ + NADPH + 3_Dehydroshikimate		 (( (( (kcat * E) ) * S2) ) / (Km + S2)) buffer:
PH: 8.8
Temperature: 22
uniprot:Q9SQT8 [shikimate dehydrogenase(Enzyme) mutant K385N, delta1-88, C-terminal his-tag]

NADP+ + Shikimate -> 3_Dehydroshikimate + NADPH + H+		 (( (( (kcat * E) ) * S1) ) / (Km + S1)) buffer:
PH: 8.8
Temperature: 22
uniprot:Q9SQT8 [shikimate dehydrogenase(Enzyme) mutant K385A, delta1-88, C-terminal his-tag]

Shikimate + NADP+ -> H+ + 3_Dehydroshikimate + NADPH		 (( (( (kcat * E) ) * S2) ) / (Km + S2)) buffer:
PH: 8.8
Temperature: 22
uniprot:Q9SQT8 [shikimate dehydrogenase(Enzyme) mutant D423N, delta1-88, C-terminal his-tag]

Shikimate + NADP+ -> NADPH + H+ + 3_Dehydroshikimate		 (( (( (kcat * E) ) * S2) ) / (Km + S2)) buffer:
PH: 8.8
Temperature: 22
uniprot:Q9SQT8 [shikimate dehydrogenase(Enzyme) mutant Y550A, delta1-88, C-terminal his-tag]

NADP+ + Shikimate -> H+ + 3_Dehydroshikimate + NADPH		 (( (( (kcat * E) ) * S2) ) / (Km + S2)) buffer:
PH: 8.8
Temperature: 22
uniprot:Q9SQT8 [shikimate dehydrogenase(Enzyme) mutant S336A, delta1-88, C-terminal his-tag]

NADP+ + Shikimate -> 3_Dehydroshikimate + NADPH + H+		 (( (( (kcat * E) ) * S2) ) / (Km + S2)) buffer:
PH: 8.8
Temperature: 22
uniprot:Q9SQT8 [shikimate dehydrogenase(Enzyme) mutant Y550F, delta1-88, C-terminal his-tag]

Shikimate + NADP+ -> NADPH + H+ + 3_Dehydroshikimate		 (( (( (kcat * E) ) * S1) ) / (Km + S1)) buffer:
PH: 8.8
Temperature: 22
uniprot:Q9SQT8 [shikimate dehydrogenase(Enzyme) mutant Y550A, delta1-88, C-terminal his-tag]

Shikimate + NADP+ -> NADPH + H+ + 3_Dehydroshikimate		 (( (( (kcat * E) ) * S1) ) / (Km + S1)) buffer:
PH: 8.8
Temperature: 22
uniprot:Q9SQT8 [shikimate dehydrogenase(Enzyme) mutant S338A, delta1-88, C-terminal his-tag]

NADP+ + Shikimate -> NADPH + H+ + 3_Dehydroshikimate		 (( (( (kcat * E) ) * S1) ) / (Km + S1)) buffer:
PH: 8.8
Temperature: 22
uniprot:Q9SQT8 [shikimate dehydrogenase(Enzyme) mutant S338A, delta1-88, C-terminal his-tag]

Shikimate + NADP+ -> H+ + 3_Dehydroshikimate + NADPH		 (( (( (kcat * E) ) * S2) ) / (Km + S2)) buffer:
PH: 8.8
Temperature: 22
uniprot:Q9SQT8 [shikimate dehydrogenase(Enzyme) mutant S336A, delta1-88, C-terminal his-tag]

NADP+ + Shikimate -> NADPH + 3_Dehydroshikimate + H+		 (( (( (kcat * E) ) * S1) ) / (Km + S1)) buffer:
PH: 8.8
Temperature: 22
uniprot:Q9SQT8 [shikimate dehydrogenase(Enzyme) mutant Y550F, delta1-88, C-terminal his-tag]

NADP+ + Shikimate -> 3_Dehydroshikimate + NADPH + H+		 (( (( (kcat * E) ) * S2) ) / (Km + S2)) buffer:
PH: 8.8
Temperature: 22
uniprot:Q9SQT8 [shikimate dehydrogenase(Enzyme) wildtype delta1-88, C-terminal his-tag]

NADP+ + Shikimate -> 3_Dehydroshikimate + H+ + NADPH		 (( (( (kcat * E) ) * S2) ) / (Km + S2)) buffer:
PH: 8.8
Temperature: 22
uniprot:Q9SQT8 [shikimate dehydrogenase(Enzyme) wildtype delta1-88, C-terminal his-tag]

NADP+ + Shikimate -> NADPH + H+ + 3_Dehydroshikimate		 (( (( (kcat * E) ) * S1) ) / (Km + S1)) buffer:
PH: 8.8
Temperature: 22
uniprot:P43876 [shikimate dehydrogenase(Enzyme) wildtype]

NADP+ + Shikimate -> 3_Dehydroshikimate + H+ + NADPH		 (( (E * kcat) ) / (1 + (( (10 ^ (0 - pH)) ) / (10 ^ (0 - pKa))))) buffer: -
PH: 7.5
Temperature: 30
uniprot:P43876 [shikimate dehydrogenase(Enzyme) wildtype]

Shikimate + NADP+ -> 3_Dehydroshikimate + H+ + NADPH		 (( (( (E * kcat) ) * B) ) / (KmB + B)) buffer: -
PH: 8.8
Temperature: 30
uniprot:P43876 [shikimate dehydrogenase(Enzyme) wildtype]

Shikimate + NADP+ -> 3_Dehydroshikimate + NADPH + H+		 (( (( (E * kcat) ) * A) ) / (KmA + A)) buffer: -
PH: 8.8
Temperature: 30
uniprot:P15770 [shikimate dehydrogenase(Enzyme) wildtype AroE]

Shikimate + NADP+ -> H+ + NADPH + 3_Dehydroshikimate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 100 mM Tris-HCl
PH: 9
Temperature: 20
uniprot:P15770 [shikimate dehydrogenase(Enzyme) wildtype AroE]

NADP+ + Shikimate -> NADPH + H+ + 3_Dehydroshikimate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 100 mM Tris-HCl
PH: 9
Temperature: 20

Pathways

pathway id name
BioCyc:META_PWY-6628 superpathway of L-phenylalanine biosynthesis
BioCyc:META_PWY-6165 chorismate biosynthesis II (archaea)
BioCyc:META_ALL-CHORISMATE-PWY superpathway of chorismate metabolism
BioCyc:ECO_COMPLETE-ARO-PWY superpathway of aromatic amino acid biosynthesis
BioCyc:YEAST_PWY-6164 3-dehydroquinate biosynthesis
BioCyc:YEAST_ARO-YEAST-PWY chorismate biosynthesis
BioCyc:VCHO_ARO-PWY chorismate biosynthesis I
BioCyc:ECOO157_PWY-6630 superpathway of tyrosine biosynthesis
BioCyc:ECOL199310_PWY-6163 chorismate biosynthesis from 3-dehydroquinate
BioCyc:ECOL199310_ARO-PWY chorismate biosynthesis I
BioCyc:ECOL199310_COMPLETE-ARO-PWY superpathway of phenylalanine, tyrosine, and tryptophan biosynthesis
BioCyc:ECOL199310_PWY-6628 superpathway of phenylalanine biosynthesis
BioCyc:BSUB_PWY-6163 chorismate biosynthesis from 3-dehydroquinate
BioCyc:ECOL316407_ALL-CHORISMATE-PWY superpathway of chorismate metabolism
BioCyc:META_PWY-6630 superpathway of L-tyrosine biosynthesis
BioCyc:ECO_ARO-PWY chorismate biosynthesis I
BioCyc:ARA_PWY-6707 gallate biosynthesis
BioCyc:VCHO_PWY-6628 superpathway of L-phenylalanine biosynthesis
BioCyc:VCHO_ALL-CHORISMATE-PWY superpathway of chorismate metabolism
BioCyc:VCHO_PWY-6629 superpathway of L-tryptophan biosynthesis
BioCyc:HPY_ARO-PWY chorismate biosynthesis I
BioCyc:ECOO157_COMPLETE-ARO-PWY superpathway of phenylalanine, tyrosine, and tryptophan biosynthesis
BioCyc:ECOO157_PWY-6629 superpathway of tryptophan biosynthesis
BioCyc:AGRO_COMPLETE-ARO-PWY superpathway of aromatic amino acid biosynthesis
BioCyc:BSUB_ARO-PWY chorismate biosynthesis I
BioCyc:PCHR_PWY-6416 quinate degradation II
BioCyc:ECOL316407_COMPLETE-ARO-PWY superpathway of phenylalanine, tyrosine, and tryptophan biosynthesis
BioCyc:ECOL316407_ARO-PWY chorismate biosynthesis I
BioCyc:GCF_000013425_PWY-6163 chorismate biosynthesis from 3-dehydroquinate
BioCyc:META_COMPLETE-ARO-PWY superpathway of aromatic amino acid biosynthesis
BioCyc:META_ARO-PWY chorismate biosynthesis I
BioCyc:META_PWY-6164 3-dehydroquinate biosynthesis I
BioCyc:META_PWY-6707 gallate biosynthesis
BioCyc:ECO_PWY-6163 chorismate biosynthesis from 3-dehydroquinate
BioCyc:YEAST_COMPLETE-ARO-YEAST-PWY superpathway of phenylalanine, tyrosine and tryptophan biosynthesis
BioCyc:VCHO_PWY-6163 chorismate biosynthesis from 3-dehydroquinate
BioCyc:ECOO157_ARO-PWY chorismate biosynthesis I
BioCyc:ECOO157_PWY-6628 superpathway of phenylalanine biosynthesis
BioCyc:ECOL199310_PWY-6629 superpathway of tryptophan biosynthesis
BioCyc:CAULO_PWY-6163 chorismate biosynthesis from 3-dehydroquinate
BioCyc:BSUB_PWY-6630 superpathway of L-tyrosine biosynthesis
BioCyc:PCHR_ARO-PWY chorismate biosynthesis I
BioCyc:GCF_000013425_PWY-6630 superpathway of L-tyrosine biosynthesis
BioCyc:META_PWY-6163 chorismate biosynthesis from 3-dehydroquinate
BioCyc:META_PWY-6629 superpathway of L-tryptophan biosynthesis
BioCyc:ECO_ALL-CHORISMATE-PWY superpathway of chorismate metabolism
BioCyc:ARA_PWY-6163 chorismate biosynthesis from 3-dehydroquinate
BioCyc:VCHO_COMPLETE-ARO-PWY superpathway of aromatic amino acid biosynthesis
BioCyc:VCHO_PWY-6630 superpathway of L-tyrosine biosynthesis
BioCyc:HPY_PWY-6163 chorismate biosynthesis from 3-dehydroquinate
BioCyc:ECOO157_PWY-6163 chorismate biosynthesis from 3-dehydroquinate
BioCyc:ECOL199310_PWY-6630 superpathway of tyrosine biosynthesis
BioCyc:ECOL199310_ALL-CHORISMATE-PWY superpathway of chorismate metabolism
BioCyc:CAULO_ARO-PWY chorismate biosynthesis I
BioCyc:AGRO_PWY-6163 chorismate biosynthesis from 3-dehydroquinate
BioCyc:AGRO_ARO-PWY chorismate biosynthesis I
BioCyc:BSUB_PWY-6629 superpathway of L-tryptophan biosynthesis
BioCyc:PCHR_PWY-6164 3-dehydroquinate biosynthesis I
BioCyc:PCHR_PWY-6163 chorismate biosynthesis from 3-dehydroquinate
BioCyc:ECOL316407_PWY-6163 chorismate biosynthesis from 3-dehydroquinate
BioCyc:CAULONA1000_COMPLETE-ARO-PWY superpathway of phenylalanine, tyrosine, and tryptophan biosynthesis
BioCyc:CAULONA1000_ARO-PWY chorismate biosynthesis
BioCyc:GCF_000013425_PWY-6629 superpathway of L-tryptophan biosynthesis
BioCyc:GCF_000013425_ARO-PWY chorismate biosynthesis I
PathBank:SMP0000836 Chorismate Biosynthesis
PathBank:SMP0001001 Secondary Metabolites: Shikimate Pathway
PlantCyc:ARA_ARO-PWY chorismate biosynthesis I
PlantCyc:PLANT_PWY-6707 gallate biosynthesis
PlantCyc:PLANT_ARO-PWY chorismate biosynthesis I
PlantCyc:ARA_PWY-6707 gallate biosynthesis
PlantCyc:ARA_PWY-6163 chorismate biosynthesis from 3-dehydroquinate
PathBank:SMP0121291 Chorismate Biosynthesis
PathBank:SMP0121335 Secondary Metabolites: Shikimate Pathway
PathBank:SMP0012009 Shikimate Pathway (Chorismate Biosynthesis)