EC: 1.14.15.18

calcidiol 1-monooxygenase (calcidiol,adrenodoxin:oxygen oxidoreductase (1-hydroxylating))

enzyme lambda metabolic reaction experiment
uniprot:Q9XS57 [Ferredoxin]

H+ + Calcidiol + O2 + NADPH -> H2O + Calcitriol + NADP+		 (( (Vmax * S) ) / (Km + S)) buffer: 0.5 mM glucose-6-phosphate, 0.1 mM NADP+, 1 U/ml glucose-6-phosphate dehydrogenase, 0.5% normal rat serum, 60 µg/ml adrenodoxin, 280 µg/ml pig kidney ferredoxin reductase
PH: 7
Temperature: 30
uniprot:O15528 [calcidiol 1-monooxygenase(Enzyme) wildtype CYP27B1 (33-508)]

O2 + Calcidiol + NADPH + H+ -> H2O + Calcitriol + NADP+		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM Tris-HCl, 0.2 µM NADPH-adrenodoxin reductase, 2 µM adrenodoxin, 1 mM EDTA
PH: 7.4
Temperature: 37
uniprot:O35084 [calcidiol 1-monooxygenase(Enzyme) wildtype CYP27B1]

O2 + Calcidiol + H+ + NADPH -> Calcitriol + H2O + NADP+		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM Tris-HCl, 0.2 µM NADPH-adrenodoxin reductase, 2 µM adrenodoxin, 1 mM EDTA
PH: 7.4
Temperature: 37
uniprot:O15528 [calcidiol 1-monooxygenase(Enzyme) wildtype CYP27B1 (33-508)]

NADPH + H+ + O2 + Secalciferol -> Calcitetrol + NADP+ + H2O		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM Tris-HCl, 0.2 µM NADPH-adrenodoxin reductase, 2 µM adrenodoxin, 1 mM EDTA
PH: 7.4
Temperature: 37
uniprot:O35084 [calcidiol 1-monooxygenase(Enzyme) wildtype CYP27B1]

H+ + Secalciferol + O2 + NADPH -> Calcitetrol + NADP+ + H2O		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM Tris-HCl, 0.2 µM NADPH-adrenodoxin reductase, 2 µM adrenodoxin, 1 mM EDTA
PH: 7.4
Temperature: 37

Pathways

pathway id name
BioCyc:MOUSE_PWY-6076 1,25-dihydroxyvitamin D3 biosynthesis
Reactome:R-HSA-196791 Vitamin D (calciferol) metabolism
Reactome:R-HSA-211859 Biological oxidations
Reactome:R-MMU-1430728 Metabolism
Reactome:R-MMU-556833 Metabolism of lipids
Reactome:R-MMU-8957322 Metabolism of steroids
Reactome:R-MMU-211859 Biological oxidations
Reactome:R-RNO-211945 Phase I - Functionalization of compounds
Reactome:R-HSA-556833 Metabolism of lipids
Reactome:R-RNO-211859 Biological oxidations
Reactome:R-HSA-211945 Phase I - Functionalization of compounds
Reactome:R-MMU-196791 Vitamin D (calciferol) metabolism
Reactome:R-MMU-211945 Phase I - Functionalization of compounds
Reactome:R-RNO-1430728 Metabolism
Reactome:R-RNO-556833 Metabolism of lipids
Reactome:R-RNO-8957322 Metabolism of steroids
Reactome:R-RNO-211897 Cytochrome P450 - arranged by substrate type
Reactome:R-RNO-211916 Vitamins
Reactome:R-HSA-1430728 Metabolism
Reactome:R-HSA-8957322 Metabolism of steroids
Reactome:R-HSA-211897 Cytochrome P450 - arranged by substrate type
Reactome:R-HSA-211916 Vitamins
Reactome:R-MMU-211897 Cytochrome P450 - arranged by substrate type
Reactome:R-MMU-211916 Vitamins
Reactome:R-RNO-196791 Vitamin D (calciferol) metabolism