cholesterol degradation to androstenedione II (cholesterol dehydrogenase)
Thomas ST, VanderVen BC, Sherman DR, Russell DG, Sampson NS. Pathway profiling in Mycobacterium tuberculosis: elucidation of cholesterol-derived catabolite and enzymes that catalyze its metabolism. J Biol Chem. 2011 Dec 23;286(51):43668–78. PMID: 22045806; PMCID: PMC3243565.; Kreit J, Sampson NS. Cholesterol oxidase: physiological functions. The FEBS Journal. 2009 Nov 16;276(23):6844–56. doi: 10.1111/j.1742-4658.2009.07378.x.; Yang X, Dubnau E, Smith I, Sampson NS. Rv1106c from Mycobacterium tuberculosis Is a 3ß-Hydroxysteroid Dehydrogenase. Biochemistry. 2007 Jul 14;46(31):9058–67. doi: 10.1021/bi700688x.
Metabolites
Enzyme
| EC Number | name | full name | note |
|---|---|---|---|
| 1.3.99.- | |||
| 1.1.1.145 | 3beta-hydroxy-DELTA5-steroid dehydrogenase | 3beta-hydroxy-DELTA5-steroid:NAD+ 3-oxidoreductase | |
| 2.3.1.16 | acetyl-CoA C-acyltransferase | acyl-CoA:acetyl-CoA C-acyltransferase | |
| 5.1.99.4 | alpha-methylacyl-CoA racemase | 2-Methylacyl-CoA 2-epimerase | |
| 5.3.3.1 | steroid DELTA-isomerase | 3-oxosteroid DELTA5-DELTA4-isomerase | |
| 6.2.1.2 | medium-chain acyl-CoA ligase | medium-chain fatty acid:CoA ligase (AMP-forming) | |
| 6.2.1.3 | long-chain-fatty-acid-CoA ligase | long-chain fatty acid:CoA ligase (AMP-forming) | |
| 6.2.1.42 | 3-oxocholest-4-en-26-oate-CoA ligase | 3-oxocholest-4-en-26-oate:CoA ligase (AMP-forming) |
Proteins
| Protein ID | name | full name |
|---|---|---|
| I6XHI0 | Rv3541c | Conserved protein |
| I6XHI4 | fadA5 | Probable acetyl-CoA acetyltransferase FadA5 (acetoacetyl-CoA thiolase) |
| I6Y3Q0 | fadE27 | Probable acyl-CoA dehydrogenase FadE27 |
| I6Y3T7 | ltp2 | Probable lipid transfer protein or keto acyl-CoA thiolase Ltp2 |
| I6YCA3 | fadE26 | Probable acyl-CoA dehydrogenase FadE26 |
| I6YGF8 | Rv3542c | Conserved hypothetical protein |
| O06543 | mcr | Probable alpha-methylacyl-CoA racemase Mcr (2-methylacyl-CoA racemase) (2-arylpropionyl-CoA epimerase) |
| O53561 | echA19 | Possible enoyl-CoA hydratase EchA19 (enoyl hydrase) (unsaturated acyl-CoA hydratase) (crotonase) |
| P71857 | fadE28 | Probable acyl-CoA dehydrogenase FadE28 |
| P71858 | fadE29 | Probable acyl-CoA dehydrogenase FadE29 |
| P96855 | fadE34 | Probable acyl-CoA dehydrogenase FadE34 |
| P9WQ51 | fadD19 | Fatty-acid-CoA ligase FadD19 (fatty-acid-CoA synthetase) (fatty-acid-CoA synthase) |
| P9WQP7 | Rv1106c | 3-beta-hydroxysteroid dehydrogenase |